anti sheep cd31 antibody  (Novus Biologicals)

Journal: Animal Models and Experimental Medicine

Article Title: Bilateral carotid‐jugular arteriovenous graft implantation in an ovine model is safe and durable for facilitation of arteriovenous graft innovation

doi: 10.1002/ame2.70096

Figure Lengend Snippet: Representative imaging of explanted AV grafts. The images include scanning electron microscopy (SEM) in the first column, immunohistochemisty (IHC) in the second column, and hematoxylin and eosin (H&E) staining in the third column. The IHC imaging includes DAPI staining (dark blue) for cell nuclei and CD31 staining (magenta) for endothelial cell junctions, which when observed in the characteristic circular ring‐like staining pattern as seen here, suggests the presence of endothelial cells. Column 3 demonstrates patent sections of graft on the left and occluded sections of the graft on the right. The image on the far‐right side of the figure demonstrates the graft sectioning technique relative to its orientation with “1” corresponding to the venous anastomosis, “2” corresponding to graft mid‐segment, and “3” corresponding to the arterial anastomosis.

Article Snippet: Samples were then incubated with mouse anti‐sheep CD31 antibody (NB100‐65900, Novus Biologicals; 1:50 in 1% BSA/PBS) at 4°C overnight.

Techniques: Imaging, Electron Microscopy, Staining

Journal: Scientific Reports

Article Title: Regional and organ-level responses to local lung irradiation in sheep

doi: 10.1038/s41598-021-88863-8

Figure Lengend Snippet: Boxplot illustrating CD31 stain expressed as a percentage of tissue area. The derivation of CD31 measurements was as described for Fig. . The data illustrates that, relative to levels seen in the control lungs of sheep undergoing an acute radiation response (CON, Acutes), there was increased expression of endothelial cell marker throughout the lungs of sheep undergoing a chronic radiation response. With the exception of one sheep (ED982), radiation exposure was associated with a decrease in expression of CD31 in the radio-exposed lung (Rx) relative to the contralateral control lung (CON).

Article Snippet: Primary antibodies CD31 (PECAM-1) clone CO.3E-1D4 (Novus Biologicals NB100-65900) and Normal Mouse IgG (Sigma M5284) were diluted to 10 μg/ml in 1:10 blocking solution (10% NDS/BSA/PBS-T diluted in BSA/PBS-T) and incubated overnight at 4 °C.

Techniques: Staining, Control, Expressing, Marker

Journal: Scientific Reports

Article Title: Regional and organ-level responses to local lung irradiation in sheep

doi: 10.1038/s41598-021-88863-8

Figure Lengend Snippet: Relationship between collagen and CD31 expression in sheep during the chronic response to radiation. For each sheep the average values for collagen (Picrosirius Red) and CD31 stain area were calculated for each radio-exposed (⊗) and contralateral control (Ο) lung and plotted against each other. A significant negative association existed between these variables (Pearson correlation = − 0.829; P-Value = 0.011).

Article Snippet: Primary antibodies CD31 (PECAM-1) clone CO.3E-1D4 (Novus Biologicals NB100-65900) and Normal Mouse IgG (Sigma M5284) were diluted to 10 μg/ml in 1:10 blocking solution (10% NDS/BSA/PBS-T diluted in BSA/PBS-T) and incubated overnight at 4 °C.

Techniques: Expressing, Staining, Control

Journal: Frontiers in Cardiovascular Medicine

Article Title: Sheep-Specific Immunohistochemical Panel for the Evaluation of Regenerative and Inflammatory Processes in Tissue-Engineered Heart Valves

doi: 10.3389/fcvm.2018.00105

Figure Lengend Snippet: Antibodies used for immunohistochemistry.

Article Snippet: CD31 (PECAM-1) , Mouse , CO.3E-1D4, IgG2a , Novus Biologicals , NB100-65900 , Pepsin , 100.

Techniques: Immunohistochemistry

Journal: Frontiers in Cardiovascular Medicine

Article Title: Sheep-Specific Immunohistochemical Panel for the Evaluation of Regenerative and Inflammatory Processes in Tissue-Engineered Heart Valves

doi: 10.3389/fcvm.2018.00105

Figure Lengend Snippet: Antibodies used for immunohistochemistry.

Article Snippet: CD31 (PECAM-1) , Mouse , CO.3E-1D4, IgG2a , Novus Biologicals , NB100-65900 , Pepsin , 100.

Techniques: Immunohistochemistry

Journal: Frontiers in Cardiovascular Medicine

Article Title: Sheep-Specific Immunohistochemical Panel for the Evaluation of Regenerative and Inflammatory Processes in Tissue-Engineered Heart Valves

doi: 10.3389/fcvm.2018.00105

Figure Lengend Snippet: Valvular endothelial cell (VEC) markers. Sections of the sheep aortic valve leaflet stained with antibodies against the endothelial cell markers CD31, CD34, VE-Cadherin (CD144), or von Willebrand Factor (vWF). Represented are the aortic wall and a section of the valvular leaflet (near the free edge), with “a” and “v” indicating the aortic and ventricular sides of the leaflet, respectively. Scale bars, 100 μm.

Article Snippet: CD31 (PECAM-1) , Mouse , CO.3E-1D4, IgG2a , Novus Biologicals , NB100-65900 , Pepsin , 100.

Techniques: Staining