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ZGW promotes remyelination in CPZ-induced demyelinated mice. ( A ) Representative LFB staining of the corpus callosum. ( B ) TEM image of the corpus callosum ultrathin sections. ( C ) Quantification of the LFB-positive area in the corpus callosum. ( D ) Myelin g-ratio (axon diameter/total fiber diameter) measured from TEM images. ( E ) Representative Western blots <t>for</t> <t>MOG,</t> Olig2, <t>CNPase,</t> and β-actin (loading control). ( F – H ) Quantification of MOG, Olig2, and CNPase normalized to β-actin. *P < 0.05, **P < 0.01, ***P < 0.001 versus CPZ, # P < 0.05, ### P < 0.001 versus NC. Data are presented as mean ± SD. (n =3 per group).
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ZGW promotes remyelination in CPZ-induced demyelinated mice. ( A ) Representative LFB staining of the corpus callosum. ( B ) TEM image of the corpus callosum ultrathin sections. ( C ) Quantification of the LFB-positive area in the corpus callosum. ( D ) Myelin g-ratio (axon diameter/total fiber diameter) measured from TEM images. ( E ) Representative Western blots <t>for</t> <t>MOG,</t> Olig2, <t>CNPase,</t> and β-actin (loading control). ( F – H ) Quantification of MOG, Olig2, and CNPase normalized to β-actin. *P < 0.05, **P < 0.01, ***P < 0.001 versus CPZ, # P < 0.05, ### P < 0.001 versus NC. Data are presented as mean ± SD. (n =3 per group).
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ZGW promotes remyelination in CPZ-induced demyelinated mice. ( A ) Representative LFB staining of the corpus callosum. ( B ) TEM image of the corpus callosum ultrathin sections. ( C ) Quantification of the LFB-positive area in the corpus callosum. ( D ) Myelin g-ratio (axon diameter/total fiber diameter) measured from TEM images. ( E ) Representative Western blots <t>for</t> <t>MOG,</t> Olig2, <t>CNPase,</t> and β-actin (loading control). ( F – H ) Quantification of MOG, Olig2, and CNPase normalized to β-actin. *P < 0.05, **P < 0.01, ***P < 0.001 versus CPZ, # P < 0.05, ### P < 0.001 versus NC. Data are presented as mean ± SD. (n =3 per group).
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ZGW promotes remyelination in CPZ-induced demyelinated mice. ( A ) Representative LFB staining of the corpus callosum. ( B ) TEM image of the corpus callosum ultrathin sections. ( C ) Quantification of the LFB-positive area in the corpus callosum. ( D ) Myelin g-ratio (axon diameter/total fiber diameter) measured from TEM images. ( E ) Representative Western blots <t>for</t> <t>MOG,</t> Olig2, <t>CNPase,</t> and β-actin (loading control). ( F – H ) Quantification of MOG, Olig2, and CNPase normalized to β-actin. *P < 0.05, **P < 0.01, ***P < 0.001 versus CPZ, # P < 0.05, ### P < 0.001 versus NC. Data are presented as mean ± SD. (n =3 per group).
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Image Search Results


ZGW promotes remyelination in CPZ-induced demyelinated mice. ( A ) Representative LFB staining of the corpus callosum. ( B ) TEM image of the corpus callosum ultrathin sections. ( C ) Quantification of the LFB-positive area in the corpus callosum. ( D ) Myelin g-ratio (axon diameter/total fiber diameter) measured from TEM images. ( E ) Representative Western blots for MOG, Olig2, CNPase, and β-actin (loading control). ( F – H ) Quantification of MOG, Olig2, and CNPase normalized to β-actin. *P < 0.05, **P < 0.01, ***P < 0.001 versus CPZ, # P < 0.05, ### P < 0.001 versus NC. Data are presented as mean ± SD. (n =3 per group).

Journal: Journal of Inflammation Research

Article Title: Zuo Gui Wan Promotes Remyelination in Multiple Sclerosis by Attenuating MAPK-Mediated Microglial M1 Polarization, an Integrated Network Pharmacology and Experimental Validation Study

doi: 10.2147/JIR.S572873

Figure Lengend Snippet: ZGW promotes remyelination in CPZ-induced demyelinated mice. ( A ) Representative LFB staining of the corpus callosum. ( B ) TEM image of the corpus callosum ultrathin sections. ( C ) Quantification of the LFB-positive area in the corpus callosum. ( D ) Myelin g-ratio (axon diameter/total fiber diameter) measured from TEM images. ( E ) Representative Western blots for MOG, Olig2, CNPase, and β-actin (loading control). ( F – H ) Quantification of MOG, Olig2, and CNPase normalized to β-actin. *P < 0.05, **P < 0.01, ***P < 0.001 versus CPZ, # P < 0.05, ### P < 0.001 versus NC. Data are presented as mean ± SD. (n =3 per group).

Article Snippet: Primary antibodies were as follows, MOG (Abcam, AB242374 ), CNPase (Santa Cruz, sc-166558), Olig2 (Merck, AB9610), Iba-1 (Hunan Aifang, AFW12391 ), β-actin (Proteintech, 66009-1-Ig), iNOS (Proteintech, 22226-1-AP), CD86 (Proteintech, 26903-1-AP), Arg-1 (Proteintech, 16001-1-AP), CD206 (Proteintech, 18704-1-AP), ERK1/2 (Proteintech, 16443-1-AP), p-ERK1/2 (Proteintech, 28733-1-AP), GAPDH (Servicebio, GB11002-100), p38 (CST, 9212S), p-p38 (CST, 4511S), JNK (CST, 9252S), and p-JNK (CST, 4668S).

Techniques: Staining, Western Blot, Control