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cluster of differentiation 31 (cd31)  (Cytelligen Inc)
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Cytelligen Inc cluster of differentiation 31 (cd31)
Cluster Of Differentiation 31 (Cd31), supplied by Cytelligen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cluster differentiation 31 cd31  (Proteintech)
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Fig. 3. MS37452 and P16KD restrain inflammatory angiogenesis in inflammatory microenvironment of TMJ. (A) A diagram showing how to collect conditioned medium (CM) from M0. M0 cells were divided into three groups: Ctrl, P16KD, and MS37452. M0 were stimulated with or without TNFα ± GP during culture for 24 h. Next, medium was changed to fresh RPMI-1640 + 10 % FBS for 24 h and M0 CM was collected. At last, M0 CM was co-cultured with HUVECs. Then, HUVECs were used for subsequent transwell assay, scratch assay, tube formation assay, western blot and RT-qPCR. (B) Representative images and quantification of HUVECs transwell assay (Ctrl: M0 VS TNFα+GP: t = 66.86, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 77.36, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 65.01, df = 4). (C) Representative images and quantification of HUVECs scratch assay (Ctrl: M0 VS TNFα+GP: t = 23.12, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 23.14, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 22.50, df = 4). (D) Representative images and quantification of HUVECs tube formation assay (Ctrl: M0 VS TNFα+GP: t = 139.4, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 115.5, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 116.8, df = 4). (E) Western blot analysis of <t>CD31</t> and VEGFA in HUVECs from different groups. (F) RT-qPCR analysis of CD31 and VEGFA in HUVECs from different groups (Relative expression of CD31 mRNA: Ctrl: M0 VS TNFα+GP: t = 66.96, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 80.36, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 86.18, df = 4. Relative expression of VEGFA mRNA: Ctrl: M0 VS TNFα+GP: t = 61.40, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 75.08, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 72.31, df = 4). Each experiment was performed three to five times. The data are presented as mean ± SD. P16KD, p16 knockdown; Ctrl, control; M0, human THP-1 derived macrophages; GP, high glucose and high palmitoleic acid. *P < 0.05 represent significant differences between the indi cated columns.
Cluster Differentiation 31 Cd31, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cluster differentiation 31 cd31 - by Bioz Stars, 2026-02
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primary mouse monoclonal antibodies against cluster of differentiation (cd) 31  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology primary mouse monoclonal antibodies against cluster of differentiation (cd) 31
Fig. 3. MS37452 and P16KD restrain inflammatory angiogenesis in inflammatory microenvironment of TMJ. (A) A diagram showing how to collect conditioned medium (CM) from M0. M0 cells were divided into three groups: Ctrl, P16KD, and MS37452. M0 were stimulated with or without TNFα ± GP during culture for 24 h. Next, medium was changed to fresh RPMI-1640 + 10 % FBS for 24 h and M0 CM was collected. At last, M0 CM was co-cultured with HUVECs. Then, HUVECs were used for subsequent transwell assay, scratch assay, tube formation assay, western blot and RT-qPCR. (B) Representative images and quantification of HUVECs transwell assay (Ctrl: M0 VS TNFα+GP: t = 66.86, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 77.36, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 65.01, df = 4). (C) Representative images and quantification of HUVECs scratch assay (Ctrl: M0 VS TNFα+GP: t = 23.12, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 23.14, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 22.50, df = 4). (D) Representative images and quantification of HUVECs tube formation assay (Ctrl: M0 VS TNFα+GP: t = 139.4, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 115.5, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 116.8, df = 4). (E) Western blot analysis of <t>CD31</t> and VEGFA in HUVECs from different groups. (F) RT-qPCR analysis of CD31 and VEGFA in HUVECs from different groups (Relative expression of CD31 mRNA: Ctrl: M0 VS TNFα+GP: t = 66.96, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 80.36, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 86.18, df = 4. Relative expression of VEGFA mRNA: Ctrl: M0 VS TNFα+GP: t = 61.40, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 75.08, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 72.31, df = 4). Each experiment was performed three to five times. The data are presented as mean ± SD. P16KD, p16 knockdown; Ctrl, control; M0, human THP-1 derived macrophages; GP, high glucose and high palmitoleic acid. *P < 0.05 represent significant differences between the indi cated columns.
Primary Mouse Monoclonal Antibodies Against Cluster Of Differentiation (Cd) 31, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary mouse monoclonal antibodies against cluster of differentiation (cd) 31/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews
primary mouse monoclonal antibodies against cluster of differentiation (cd) 31 - by Bioz Stars, 2026-02
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primary antibodies against cluster of differentiation 31  (Millipore)
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Millipore primary antibodies against cluster of differentiation 31
Fig. 3. MS37452 and P16KD restrain inflammatory angiogenesis in inflammatory microenvironment of TMJ. (A) A diagram showing how to collect conditioned medium (CM) from M0. M0 cells were divided into three groups: Ctrl, P16KD, and MS37452. M0 were stimulated with or without TNFα ± GP during culture for 24 h. Next, medium was changed to fresh RPMI-1640 + 10 % FBS for 24 h and M0 CM was collected. At last, M0 CM was co-cultured with HUVECs. Then, HUVECs were used for subsequent transwell assay, scratch assay, tube formation assay, western blot and RT-qPCR. (B) Representative images and quantification of HUVECs transwell assay (Ctrl: M0 VS TNFα+GP: t = 66.86, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 77.36, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 65.01, df = 4). (C) Representative images and quantification of HUVECs scratch assay (Ctrl: M0 VS TNFα+GP: t = 23.12, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 23.14, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 22.50, df = 4). (D) Representative images and quantification of HUVECs tube formation assay (Ctrl: M0 VS TNFα+GP: t = 139.4, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 115.5, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 116.8, df = 4). (E) Western blot analysis of <t>CD31</t> and VEGFA in HUVECs from different groups. (F) RT-qPCR analysis of CD31 and VEGFA in HUVECs from different groups (Relative expression of CD31 mRNA: Ctrl: M0 VS TNFα+GP: t = 66.96, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 80.36, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 86.18, df = 4. Relative expression of VEGFA mRNA: Ctrl: M0 VS TNFα+GP: t = 61.40, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 75.08, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 72.31, df = 4). Each experiment was performed three to five times. The data are presented as mean ± SD. P16KD, p16 knockdown; Ctrl, control; M0, human THP-1 derived macrophages; GP, high glucose and high palmitoleic acid. *P < 0.05 represent significant differences between the indi cated columns.
Primary Antibodies Against Cluster Of Differentiation 31, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary antibodies against cluster of differentiation 31/product/Millipore
Average 90 stars, based on 1 article reviews
primary antibodies against cluster of differentiation 31 - by Bioz Stars, 2026-02
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cluster of differentiation 31 cd31 antibody  (Proteintech)
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Proteintech cluster of differentiation 31 cd31 antibody
Fig. 3. MS37452 and P16KD restrain inflammatory angiogenesis in inflammatory microenvironment of TMJ. (A) A diagram showing how to collect conditioned medium (CM) from M0. M0 cells were divided into three groups: Ctrl, P16KD, and MS37452. M0 were stimulated with or without TNFα ± GP during culture for 24 h. Next, medium was changed to fresh RPMI-1640 + 10 % FBS for 24 h and M0 CM was collected. At last, M0 CM was co-cultured with HUVECs. Then, HUVECs were used for subsequent transwell assay, scratch assay, tube formation assay, western blot and RT-qPCR. (B) Representative images and quantification of HUVECs transwell assay (Ctrl: M0 VS TNFα+GP: t = 66.86, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 77.36, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 65.01, df = 4). (C) Representative images and quantification of HUVECs scratch assay (Ctrl: M0 VS TNFα+GP: t = 23.12, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 23.14, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 22.50, df = 4). (D) Representative images and quantification of HUVECs tube formation assay (Ctrl: M0 VS TNFα+GP: t = 139.4, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 115.5, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 116.8, df = 4). (E) Western blot analysis of <t>CD31</t> and VEGFA in HUVECs from different groups. (F) RT-qPCR analysis of CD31 and VEGFA in HUVECs from different groups (Relative expression of CD31 mRNA: Ctrl: M0 VS TNFα+GP: t = 66.96, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 80.36, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 86.18, df = 4. Relative expression of VEGFA mRNA: Ctrl: M0 VS TNFα+GP: t = 61.40, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 75.08, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 72.31, df = 4). Each experiment was performed three to five times. The data are presented as mean ± SD. P16KD, p16 knockdown; Ctrl, control; M0, human THP-1 derived macrophages; GP, high glucose and high palmitoleic acid. *P < 0.05 represent significant differences between the indi cated columns.
Cluster Of Differentiation 31 Cd31 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cluster of differentiation 31 cd31 antibody/product/Proteintech
Average 90 stars, based on 1 article reviews
cluster of differentiation 31 cd31 antibody - by Bioz Stars, 2026-02
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cluster of differentiation (cd)31 antibody  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc cluster of differentiation (cd)31 antibody
Fig. 3. MS37452 and P16KD restrain inflammatory angiogenesis in inflammatory microenvironment of TMJ. (A) A diagram showing how to collect conditioned medium (CM) from M0. M0 cells were divided into three groups: Ctrl, P16KD, and MS37452. M0 were stimulated with or without TNFα ± GP during culture for 24 h. Next, medium was changed to fresh RPMI-1640 + 10 % FBS for 24 h and M0 CM was collected. At last, M0 CM was co-cultured with HUVECs. Then, HUVECs were used for subsequent transwell assay, scratch assay, tube formation assay, western blot and RT-qPCR. (B) Representative images and quantification of HUVECs transwell assay (Ctrl: M0 VS TNFα+GP: t = 66.86, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 77.36, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 65.01, df = 4). (C) Representative images and quantification of HUVECs scratch assay (Ctrl: M0 VS TNFα+GP: t = 23.12, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 23.14, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 22.50, df = 4). (D) Representative images and quantification of HUVECs tube formation assay (Ctrl: M0 VS TNFα+GP: t = 139.4, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 115.5, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 116.8, df = 4). (E) Western blot analysis of <t>CD31</t> and VEGFA in HUVECs from different groups. (F) RT-qPCR analysis of CD31 and VEGFA in HUVECs from different groups (Relative expression of CD31 mRNA: Ctrl: M0 VS TNFα+GP: t = 66.96, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 80.36, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 86.18, df = 4. Relative expression of VEGFA mRNA: Ctrl: M0 VS TNFα+GP: t = 61.40, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 75.08, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 72.31, df = 4). Each experiment was performed three to five times. The data are presented as mean ± SD. P16KD, p16 knockdown; Ctrl, control; M0, human THP-1 derived macrophages; GP, high glucose and high palmitoleic acid. *P < 0.05 represent significant differences between the indi cated columns.
Cluster Of Differentiation (Cd)31 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cluster of differentiation (cd)31 antibody/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
cluster of differentiation (cd)31 antibody - by Bioz Stars, 2026-02
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antibodies detecting cluster of differentiation (cd) 31  (Cell Marque)
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Cell Marque antibodies detecting cluster of differentiation (cd) 31
Fig. 3. MS37452 and P16KD restrain inflammatory angiogenesis in inflammatory microenvironment of TMJ. (A) A diagram showing how to collect conditioned medium (CM) from M0. M0 cells were divided into three groups: Ctrl, P16KD, and MS37452. M0 were stimulated with or without TNFα ± GP during culture for 24 h. Next, medium was changed to fresh RPMI-1640 + 10 % FBS for 24 h and M0 CM was collected. At last, M0 CM was co-cultured with HUVECs. Then, HUVECs were used for subsequent transwell assay, scratch assay, tube formation assay, western blot and RT-qPCR. (B) Representative images and quantification of HUVECs transwell assay (Ctrl: M0 VS TNFα+GP: t = 66.86, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 77.36, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 65.01, df = 4). (C) Representative images and quantification of HUVECs scratch assay (Ctrl: M0 VS TNFα+GP: t = 23.12, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 23.14, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 22.50, df = 4). (D) Representative images and quantification of HUVECs tube formation assay (Ctrl: M0 VS TNFα+GP: t = 139.4, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 115.5, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 116.8, df = 4). (E) Western blot analysis of <t>CD31</t> and VEGFA in HUVECs from different groups. (F) RT-qPCR analysis of CD31 and VEGFA in HUVECs from different groups (Relative expression of CD31 mRNA: Ctrl: M0 VS TNFα+GP: t = 66.96, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 80.36, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 86.18, df = 4. Relative expression of VEGFA mRNA: Ctrl: M0 VS TNFα+GP: t = 61.40, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 75.08, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 72.31, df = 4). Each experiment was performed three to five times. The data are presented as mean ± SD. P16KD, p16 knockdown; Ctrl, control; M0, human THP-1 derived macrophages; GP, high glucose and high palmitoleic acid. *P < 0.05 represent significant differences between the indi cated columns.
Antibodies Detecting Cluster Of Differentiation (Cd) 31, supplied by Cell Marque, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibodies detecting cluster of differentiation (cd) 31 - by Bioz Stars, 2026-02
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cluster of differentiation 31 (cd31) antibody  (Huabio Inc)
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Huabio Inc cluster of differentiation 31 (cd31) antibody
Fig. 3. MS37452 and P16KD restrain inflammatory angiogenesis in inflammatory microenvironment of TMJ. (A) A diagram showing how to collect conditioned medium (CM) from M0. M0 cells were divided into three groups: Ctrl, P16KD, and MS37452. M0 were stimulated with or without TNFα ± GP during culture for 24 h. Next, medium was changed to fresh RPMI-1640 + 10 % FBS for 24 h and M0 CM was collected. At last, M0 CM was co-cultured with HUVECs. Then, HUVECs were used for subsequent transwell assay, scratch assay, tube formation assay, western blot and RT-qPCR. (B) Representative images and quantification of HUVECs transwell assay (Ctrl: M0 VS TNFα+GP: t = 66.86, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 77.36, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 65.01, df = 4). (C) Representative images and quantification of HUVECs scratch assay (Ctrl: M0 VS TNFα+GP: t = 23.12, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 23.14, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 22.50, df = 4). (D) Representative images and quantification of HUVECs tube formation assay (Ctrl: M0 VS TNFα+GP: t = 139.4, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 115.5, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 116.8, df = 4). (E) Western blot analysis of <t>CD31</t> and VEGFA in HUVECs from different groups. (F) RT-qPCR analysis of CD31 and VEGFA in HUVECs from different groups (Relative expression of CD31 mRNA: Ctrl: M0 VS TNFα+GP: t = 66.96, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 80.36, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 86.18, df = 4. Relative expression of VEGFA mRNA: Ctrl: M0 VS TNFα+GP: t = 61.40, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 75.08, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 72.31, df = 4). Each experiment was performed three to five times. The data are presented as mean ± SD. P16KD, p16 knockdown; Ctrl, control; M0, human THP-1 derived macrophages; GP, high glucose and high palmitoleic acid. *P < 0.05 represent significant differences between the indi cated columns.
Cluster Of Differentiation 31 (Cd31) Antibody, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cluster of differentiation 31 (cd31) antibody/product/Huabio Inc
Average 90 stars, based on 1 article reviews
cluster of differentiation 31 (cd31) antibody - by Bioz Stars, 2026-02
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cluster differentiation 31 (cd31  (Biozol Diagnostica Vertrieb GmbH)
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Biozol Diagnostica Vertrieb GmbH cluster differentiation 31 (cd31
Fig. 3. MS37452 and P16KD restrain inflammatory angiogenesis in inflammatory microenvironment of TMJ. (A) A diagram showing how to collect conditioned medium (CM) from M0. M0 cells were divided into three groups: Ctrl, P16KD, and MS37452. M0 were stimulated with or without TNFα ± GP during culture for 24 h. Next, medium was changed to fresh RPMI-1640 + 10 % FBS for 24 h and M0 CM was collected. At last, M0 CM was co-cultured with HUVECs. Then, HUVECs were used for subsequent transwell assay, scratch assay, tube formation assay, western blot and RT-qPCR. (B) Representative images and quantification of HUVECs transwell assay (Ctrl: M0 VS TNFα+GP: t = 66.86, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 77.36, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 65.01, df = 4). (C) Representative images and quantification of HUVECs scratch assay (Ctrl: M0 VS TNFα+GP: t = 23.12, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 23.14, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 22.50, df = 4). (D) Representative images and quantification of HUVECs tube formation assay (Ctrl: M0 VS TNFα+GP: t = 139.4, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 115.5, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 116.8, df = 4). (E) Western blot analysis of <t>CD31</t> and VEGFA in HUVECs from different groups. (F) RT-qPCR analysis of CD31 and VEGFA in HUVECs from different groups (Relative expression of CD31 mRNA: Ctrl: M0 VS TNFα+GP: t = 66.96, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 80.36, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 86.18, df = 4. Relative expression of VEGFA mRNA: Ctrl: M0 VS TNFα+GP: t = 61.40, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 75.08, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 72.31, df = 4). Each experiment was performed three to five times. The data are presented as mean ± SD. P16KD, p16 knockdown; Ctrl, control; M0, human THP-1 derived macrophages; GP, high glucose and high palmitoleic acid. *P < 0.05 represent significant differences between the indi cated columns.
Cluster Differentiation 31 (Cd31, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cluster differentiation 31 (cd31/product/Biozol Diagnostica Vertrieb GmbH
Average 90 stars, based on 1 article reviews
cluster differentiation 31 (cd31 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

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Fig. 3. MS37452 and P16KD restrain inflammatory angiogenesis in inflammatory microenvironment of TMJ. (A) A diagram showing how to collect conditioned medium (CM) from M0. M0 cells were divided into three groups: Ctrl, P16KD, and MS37452. M0 were stimulated with or without TNFα ± GP during culture for 24 h. Next, medium was changed to fresh RPMI-1640 + 10 % FBS for 24 h and M0 CM was collected. At last, M0 CM was co-cultured with HUVECs. Then, HUVECs were used for subsequent transwell assay, scratch assay, tube formation assay, western blot and RT-qPCR. (B) Representative images and quantification of HUVECs transwell assay (Ctrl: M0 VS TNFα+GP: t = 66.86, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 77.36, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 65.01, df = 4). (C) Representative images and quantification of HUVECs scratch assay (Ctrl: M0 VS TNFα+GP: t = 23.12, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 23.14, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 22.50, df = 4). (D) Representative images and quantification of HUVECs tube formation assay (Ctrl: M0 VS TNFα+GP: t = 139.4, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 115.5, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 116.8, df = 4). (E) Western blot analysis of CD31 and VEGFA in HUVECs from different groups. (F) RT-qPCR analysis of CD31 and VEGFA in HUVECs from different groups (Relative expression of CD31 mRNA: Ctrl: M0 VS TNFα+GP: t = 66.96, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 80.36, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 86.18, df = 4. Relative expression of VEGFA mRNA: Ctrl: M0 VS TNFα+GP: t = 61.40, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 75.08, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 72.31, df = 4). Each experiment was performed three to five times. The data are presented as mean ± SD. P16KD, p16 knockdown; Ctrl, control; M0, human THP-1 derived macrophages; GP, high glucose and high palmitoleic acid. *P < 0.05 represent significant differences between the indi cated columns.

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: p16INK4a downregulation alleviates temporomandibular joint osteoarthritis combined with type 2 diabetes by driving M2 polarization.

doi: 10.1016/j.biopha.2025.118172

Figure Lengend Snippet: Fig. 3. MS37452 and P16KD restrain inflammatory angiogenesis in inflammatory microenvironment of TMJ. (A) A diagram showing how to collect conditioned medium (CM) from M0. M0 cells were divided into three groups: Ctrl, P16KD, and MS37452. M0 were stimulated with or without TNFα ± GP during culture for 24 h. Next, medium was changed to fresh RPMI-1640 + 10 % FBS for 24 h and M0 CM was collected. At last, M0 CM was co-cultured with HUVECs. Then, HUVECs were used for subsequent transwell assay, scratch assay, tube formation assay, western blot and RT-qPCR. (B) Representative images and quantification of HUVECs transwell assay (Ctrl: M0 VS TNFα+GP: t = 66.86, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 77.36, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 65.01, df = 4). (C) Representative images and quantification of HUVECs scratch assay (Ctrl: M0 VS TNFα+GP: t = 23.12, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 23.14, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 22.50, df = 4). (D) Representative images and quantification of HUVECs tube formation assay (Ctrl: M0 VS TNFα+GP: t = 139.4, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 115.5, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 116.8, df = 4). (E) Western blot analysis of CD31 and VEGFA in HUVECs from different groups. (F) RT-qPCR analysis of CD31 and VEGFA in HUVECs from different groups (Relative expression of CD31 mRNA: Ctrl: M0 VS TNFα+GP: t = 66.96, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 80.36, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 86.18, df = 4. Relative expression of VEGFA mRNA: Ctrl: M0 VS TNFα+GP: t = 61.40, df = 4. Ctrl-TNFα+GP VS P16KD-TNFα+GP: t = 75.08, df = 4. Ctrl-TNFα+GP VS MS37452-TNFα+GP: t = 72.31, df = 4). Each experiment was performed three to five times. The data are presented as mean ± SD. P16KD, p16 knockdown; Ctrl, control; M0, human THP-1 derived macrophages; GP, high glucose and high palmitoleic acid. *P < 0.05 represent significant differences between the indi cated columns.

Article Snippet: After that, membranes were incubated at 4 ◦C overnight with primary antibodies against inducible nitric oxide synthase (INOS) (Proteintech Group, Rosemont, IL, USA), arginase 1 (Arg1) (Proteintech Group, Rosemont, IL, USA), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech Group, Rosemont, IL, USA), transferrin receptor (TFRC) (Proteintech Group, Rosemont, IL, USA), vascular endothelial growth factor A (VEGFA) (Proteintech Group, Rosemont, IL, USA), alkaline phosphatase (ALP) (Proteintech Group, Rosemont, IL, USA), runt-related transcription factor 2 (RUNX2) (Proteintech Group, Rosemont, IL, USA), cluster differentiation 31 (CD31) (Proteintech Group, Rosemont, IL, USA), ferritin heavy chain (FTH1) (Proteintech Group, Rosemont, IL, USA).

Techniques: Cell Culture, Transwell Assay, Wound Healing Assay, Tube Formation Assay, Western Blot, Quantitative RT-PCR, Expressing, Knockdown, Control, Derivative Assay