cls iii collagenase  (Worthington Biochemical)


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  • 99
    Name:
    Collagenase Type 3
    Description:
    Lower in secondary proteolytic contaminant activities but with typical collagenase activity Suggested for mammary primary cell isolation A dialyzed lyophilized powder
    Catalog Number:
    LS004180
    Price:
    35
    Source:
    Clostridium histolyticum
    Size:
    100 mg
    Cas Number:
    9001.12.1
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    Structured Review

    Worthington Biochemical cls iii collagenase
    Lower in secondary proteolytic contaminant activities but with typical collagenase activity Suggested for mammary primary cell isolation A dialyzed lyophilized powder
    https://www.bioz.com/result/cls iii collagenase/product/Worthington Biochemical
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cls iii collagenase - by Bioz Stars, 2021-09
    99/100 stars

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    Related Articles

    other:

    Article Title: CD147 receptor is essential for TFF3-mediated signaling regulating colorectal cancer progression
    Article Snippet: The pieces were digested in DMEM supplemented with 2 mg/mL collagenase III (Worthington Biochemical Corp.) at 37 °C and with 5% CO2 for 5 h with occasional shaking.

    Article Title: Integrated light and electron microscopy continuum resolution imaging of 3D cell cultures
    Article Snippet: For dissociation of the tissue, the mammary glands were digested overnight at 37 °C and 5% CO2 in a loosely capped 50 ml falcon with 5 ml of DMEM/F12 (Lonza) supplemented with 25 mM HEPES, 1% Penicillin Streptomycin solution (ThermoFisher), 750 units of Collagenase Type III (Worthington Biochemical Corporation) and 20 µg of Liberase TM (Roche).

    Article Title: Acid-base transporters and pH dynamics in human breast carcinomas predict proliferative activity, metastasis, and survival
    Article Snippet: The tissue samples were first cut into 1 mm pieces in phosphate-buffered saline and then transferred to Tissue Culture Flat Tubes (Techno Plastic Products AG, Switzerland) containing advanced DMEM/F12 culture medium (Life Technologies, Denmark) added 10% fetal bovine serum (Biochrom AG, Germany), 1% GlutaMAX (Thermo Fisher Scientific, Denmark) and 450 IU/mL collagenase type 3 (Worthington Biochemical Corporation, Lakewood, NJ).

    Article Title: Human endometrium-derived stem cell improves cardiac function after myocardial ischemic injury by enhancing angiogenesis and myocardial metabolism
    Article Snippet: Briefly, the endometrium was minced and suspended in PBS, containing collagenase type 3 (300 μg/ml; Worthington Biochemical Corp, Freehold, NJ) and 40 μg/ml of deoxyribonuclease type I (Worthington Biochemical Corp.).

    Article Title: Baicalin Promotes Mammary Gland Development via Steroid-Like Activities
    Article Snippet: Minced tissue was placed in culture medium [RPMI 1640 with 25 mM HEPES, 5% fetal bovine serum (FBS), 1% penicillin-streptomycin-glutamine, 300 U/ml collagenase III (Worthington)] and digested for 2 h at 37°C.

    Mouse Assay:

    Article Title: Arthritis Flares Mediated by Tissue Resident Memory T Cells in the Joint
    Article Snippet: .. Murine synovium processingSynovium was dissected from the knees and ankles of mice and digested for 30 minutes with DNase I 0.1mg/mL (Sigma-Aldrich) and Collagenase type IV 1mg/mL (Worthington) in RPMI at 37°C. ..

    Isolation:

    Article Title: The dual distinct role of telomerase in repression of senescence and myofibroblast differentiation
    Article Snippet: .. Mouse lung fibroblasts (MLF) were isolated with a digestion cocktail containing collagenase III and DNase I (Worthington Biochemical Crop., Lakewood, NJ, USA), and maintained in DMEM supplemented with 10% plasma-derived fetal bovine serum (PDS; Animal Technologies, Tyler, TX, USA), 10 ng/ml of EGF and 5 ng/ml of PDGF (R & D systems, Inc. Minneapolis, MN, USA) as before [ ]. ..

    Incubation:

    Article Title: Relationships of Alzheimer’s disease and apolipoprotein E genotypes with small RNA and protein cargo of brain tissue extracellular vesicles
    Article Snippet: .. The remaining frozen tissue was weighed and briefly sliced on dry ice and then incubated in 75 U/ml collagenase type 3 (Worthington #CLS-3, S8P18814) in Hibernate-E solution for 20 min at 37°C. ..

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