Review



aiptasia ( sensu exaiptasia diaphana )  (Carolina Biological)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Carolina Biological aiptasia ( sensu exaiptasia diaphana )
    Anemones in two separate CBASS systems show highly similar thermal tolerance thresholds. (A) Schematic representation of the short-term acute heat stress assay using the Coral Bleaching Automated Stress System (CBASS; adapted from ). The 18 h CBASS profile was run at 30 °C (control) and with a 3 h heat-hold at 34 °C (control +4 °C, medium), 36 °C (control +6 °C, high), and 39 °C (control +9 °C, extreme). Dark-acclimated photosynthetic efficiency was measured using a PAM fluorometer 1 h after temperature ramp down and accompanying dark acclimation (7 h, PAM 1). (B) Photosynthetic efficiencies (F v /F m ) across replicated CBASS assays of <t>Aiptasia</t> strains F003 (dark and light purple) and H2 (yellow and orange) using two independent CBASS systems (system A and system B). Population ED50 thermal tolerance thresholds (n = 10 anemones per temperature profile) are denoted as vertical lines. Population ED50s are based on log-logistic regression curves of F v /F m measurements across experimental temperatures and provide a standardized proxy for bleaching susceptibility . ED50s and regression curves are largely identical and reproducible between the replicated CBASS runs.
    Aiptasia ( Sensu Exaiptasia Diaphana ), supplied by Carolina Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aiptasia ( sensu exaiptasia diaphana )/product/Carolina Biological
    Average 90 stars, based on 1 article reviews
    aiptasia ( sensu exaiptasia diaphana ) - by Bioz Stars, 2025-11
    90/100 stars

    Images

    1) Product Images from "Short-term heat stress assays provide a standardized screening tool to assess impacts of microbiome manipulation on Aiptasia thermal stress tolerance"

    Article Title: Short-term heat stress assays provide a standardized screening tool to assess impacts of microbiome manipulation on Aiptasia thermal stress tolerance

    Journal: bioRxiv

    doi: 10.1101/2023.06.01.543236

    Anemones in two separate CBASS systems show highly similar thermal tolerance thresholds. (A) Schematic representation of the short-term acute heat stress assay using the Coral Bleaching Automated Stress System (CBASS; adapted from ). The 18 h CBASS profile was run at 30 °C (control) and with a 3 h heat-hold at 34 °C (control +4 °C, medium), 36 °C (control +6 °C, high), and 39 °C (control +9 °C, extreme). Dark-acclimated photosynthetic efficiency was measured using a PAM fluorometer 1 h after temperature ramp down and accompanying dark acclimation (7 h, PAM 1). (B) Photosynthetic efficiencies (F v /F m ) across replicated CBASS assays of Aiptasia strains F003 (dark and light purple) and H2 (yellow and orange) using two independent CBASS systems (system A and system B). Population ED50 thermal tolerance thresholds (n = 10 anemones per temperature profile) are denoted as vertical lines. Population ED50s are based on log-logistic regression curves of F v /F m measurements across experimental temperatures and provide a standardized proxy for bleaching susceptibility . ED50s and regression curves are largely identical and reproducible between the replicated CBASS runs.
    Figure Legend Snippet: Anemones in two separate CBASS systems show highly similar thermal tolerance thresholds. (A) Schematic representation of the short-term acute heat stress assay using the Coral Bleaching Automated Stress System (CBASS; adapted from ). The 18 h CBASS profile was run at 30 °C (control) and with a 3 h heat-hold at 34 °C (control +4 °C, medium), 36 °C (control +6 °C, high), and 39 °C (control +9 °C, extreme). Dark-acclimated photosynthetic efficiency was measured using a PAM fluorometer 1 h after temperature ramp down and accompanying dark acclimation (7 h, PAM 1). (B) Photosynthetic efficiencies (F v /F m ) across replicated CBASS assays of Aiptasia strains F003 (dark and light purple) and H2 (yellow and orange) using two independent CBASS systems (system A and system B). Population ED50 thermal tolerance thresholds (n = 10 anemones per temperature profile) are denoted as vertical lines. Population ED50s are based on log-logistic regression curves of F v /F m measurements across experimental temperatures and provide a standardized proxy for bleaching susceptibility . ED50s and regression curves are largely identical and reproducible between the replicated CBASS runs.

    Techniques Used:

    (A) Schematic representation of repeat CBASS runs. The same set of F003 and H2 anemones (n = 10 per temperature profile) were subjected to repeat CBASS runs on consecutive days (CBASS 1 and CBASS 2) as well as after one month under ambient rearing conditions (CBASS 3). PAM fluorometry measurement time points and CBASS cycles are indicated on the x-axis. (B) Population ED50 thermal tolerance thresholds based on photosynthetic efficiency (F v /F m ) measures of Aiptasia strains F003 and H2 across three time points. ED50 thermal tolerance thresholds decrease with repeated stress testing but increase again after subsequent rearing under ambient conditions, although not to the initial level. Error bars represent the standard error of the population ED50s. (C) Photosynthetic efficiencies (F v /F m ) across treatment temperatures for the three CBASS runs for F003 and H2 anemones. Please refer to (B) for an overview of the time points. Population ED50 thermal tolerance thresholds based on log-logistic regression curves of F v /F m measurements are denoted as vertical lines.
    Figure Legend Snippet: (A) Schematic representation of repeat CBASS runs. The same set of F003 and H2 anemones (n = 10 per temperature profile) were subjected to repeat CBASS runs on consecutive days (CBASS 1 and CBASS 2) as well as after one month under ambient rearing conditions (CBASS 3). PAM fluorometry measurement time points and CBASS cycles are indicated on the x-axis. (B) Population ED50 thermal tolerance thresholds based on photosynthetic efficiency (F v /F m ) measures of Aiptasia strains F003 and H2 across three time points. ED50 thermal tolerance thresholds decrease with repeated stress testing but increase again after subsequent rearing under ambient conditions, although not to the initial level. Error bars represent the standard error of the population ED50s. (C) Photosynthetic efficiencies (F v /F m ) across treatment temperatures for the three CBASS runs for F003 and H2 anemones. Please refer to (B) for an overview of the time points. Population ED50 thermal tolerance thresholds based on log-logistic regression curves of F v /F m measurements are denoted as vertical lines.

    Techniques Used:

    (A) Schematic representation of microbiome manipulation through bacterial inoculation with V. coralliilyticus and subsequent CBASS testing. Inoculation was started 24 h prior to the CBASS assay and continued for the duration of the run. The PAM fluorometry measurement time point and CBASS profile are indicated on the x-axis. (B) Population ED50 thermal tolerance thresholds based on photosynthetic efficiency (F v /F m ) measures of untreated control and bacteria-inoculated F003 and H2 anemones. Population ED50 thermal tolerance thresholds of both Aiptasia strains decrease drastically after heat stress subsequent to Vibrio inoculation. Error bars represent the standard error of the population ED50s (Control, n = 10 anemones per temperature profile; Vibrio , n = 5 anemones per temperature profile). (C) Photosynthetic efficiencies (F v /F m ) of untreated control and Vibrio -inoculated anemones across CBASS assays of F003 and H2 anemones. Population ED50 thermal tolerance thresholds based on log-logistic regression curves of F v /F m measurements are denoted as vertical lines.
    Figure Legend Snippet: (A) Schematic representation of microbiome manipulation through bacterial inoculation with V. coralliilyticus and subsequent CBASS testing. Inoculation was started 24 h prior to the CBASS assay and continued for the duration of the run. The PAM fluorometry measurement time point and CBASS profile are indicated on the x-axis. (B) Population ED50 thermal tolerance thresholds based on photosynthetic efficiency (F v /F m ) measures of untreated control and bacteria-inoculated F003 and H2 anemones. Population ED50 thermal tolerance thresholds of both Aiptasia strains decrease drastically after heat stress subsequent to Vibrio inoculation. Error bars represent the standard error of the population ED50s (Control, n = 10 anemones per temperature profile; Vibrio , n = 5 anemones per temperature profile). (C) Photosynthetic efficiencies (F v /F m ) of untreated control and Vibrio -inoculated anemones across CBASS assays of F003 and H2 anemones. Population ED50 thermal tolerance thresholds based on log-logistic regression curves of F v /F m measurements are denoted as vertical lines.

    Techniques Used:



    Similar Products

    Image Search Results