materials 2 ethynyl adenosine (Jena Bioscience)
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Name:
2 Ethynyl adenosine
Description:
2 Ethynyl labeled adenosine 2 EA can be used to measure de novo mRNA poly A tail synthesis in proliferating cells 2 EA is cell permeable and incorporates into nascent mRNA transcripts both transcriptionally by RNA polymerase I II and III and posttranscriptionally by poly A polymerase instead of their natural analolg adenosine The resulting ethynyl functionalized RNA can subsequently be detected via Cu I catalyzed click chemistry that offers the choice to introduce a Biotin group via Azides of Biotin for subsequent purification tasks or a fluorescent group via Azides of fluorescent dyes for subsequent microscopic imaging 1 Presolski et al 2 and Hong et al 3 provide a general protocol for Cu I catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays
Catalog Number:
clk-n005-1
Molecular Weight:
291.26 g/mol
Price:
75.54
Applications:
mRNA poly(A) tail synthesis monitoring[1]
Purity:
≥ 95 % (HPLC)
Category:
Click Chemistry
Format:
solid
Formula:
C12H13N5O4
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Jena Bioscience
materials 2 ethynyl adenosine
2 Ethynyl labeled adenosine 2 EA can be used to measure de novo mRNA poly A tail synthesis in proliferating cells 2 EA is cell permeable and incorporates into nascent mRNA transcripts both transcriptionally by RNA polymerase I II and III and posttranscriptionally by poly A polymerase instead of their natural analolg adenosine The resulting ethynyl functionalized RNA can subsequently be detected via Cu I catalyzed click chemistry that offers the choice to introduce a Biotin group via Azides of Biotin for subsequent purification tasks or a fluorescent group via Azides of fluorescent dyes for subsequent microscopic imaging 1 Presolski et al 2 and Hong et al 3 provide a general protocol for Cu I catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays
https://www.bioz.com/result/materials 2 ethynyl adenosine/product/Jena Bioscience
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
2 Ethynyl labeled adenosine 2 EA can be used to measure de novo mRNA poly A tail synthesis in proliferating cells 2 EA is cell permeable and incorporates into nascent mRNA transcripts both transcriptionally by RNA polymerase I II and III and posttranscriptionally by poly A polymerase instead of their natural analolg adenosine The resulting ethynyl functionalized RNA can subsequently be detected via Cu I catalyzed click chemistry that offers the choice to introduce a Biotin group via Azides of Biotin for subsequent purification tasks or a fluorescent group via Azides of fluorescent dyes for subsequent microscopic imaging 1 Presolski et al 2 and Hong et al 3 provide a general protocol for Cu I catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays
https://www.bioz.com/result/materials 2 ethynyl adenosine/product/Jena Bioscience
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
materials 2 ethynyl adenosine - by Bioz Stars,
2020-07
93/100 stars
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other:Article Title: Click-encoded rolling FISH for visualizing single-cell RNA polyadenylation and structures Article Snippet: Materials 2-Ethynyl-adenosine (2-EA) and |