materials 2 ethynyl adenosine  (Jena Bioscience)


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    Name:
    2 Ethynyl adenosine
    Description:
    2 Ethynyl labeled adenosine 2 EA can be used to measure de novo mRNA poly A tail synthesis in proliferating cells 2 EA is cell permeable and incorporates into nascent mRNA transcripts both transcriptionally by RNA polymerase I II and III and posttranscriptionally by poly A polymerase instead of their natural analolg adenosine The resulting ethynyl functionalized RNA can subsequently be detected via Cu I catalyzed click chemistry that offers the choice to introduce a Biotin group via Azides of Biotin for subsequent purification tasks or a fluorescent group via Azides of fluorescent dyes for subsequent microscopic imaging 1 Presolski et al 2 and Hong et al 3 provide a general protocol for Cu I catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays
    Catalog Number:
    clk-n005-1
    Molecular Weight:
    291.26 g/mol
    Price:
    75.54
    Applications:
    mRNA poly(A) tail synthesis monitoring[1]
    Purity:
    ≥ 95 % (HPLC)
    Category:
    Click Chemistry
    Format:
    solid
    Formula:
    C12H13N5O4
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    Structured Review

    Jena Bioscience materials 2 ethynyl adenosine
    2 Ethynyl labeled adenosine 2 EA can be used to measure de novo mRNA poly A tail synthesis in proliferating cells 2 EA is cell permeable and incorporates into nascent mRNA transcripts both transcriptionally by RNA polymerase I II and III and posttranscriptionally by poly A polymerase instead of their natural analolg adenosine The resulting ethynyl functionalized RNA can subsequently be detected via Cu I catalyzed click chemistry that offers the choice to introduce a Biotin group via Azides of Biotin for subsequent purification tasks or a fluorescent group via Azides of fluorescent dyes for subsequent microscopic imaging 1 Presolski et al 2 and Hong et al 3 provide a general protocol for Cu I catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays
    https://www.bioz.com/result/materials 2 ethynyl adenosine/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    materials 2 ethynyl adenosine - by Bioz Stars, 2020-07
    93/100 stars

    Related Products / Commonly Used Together

    2-ethynyl-atp

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    other:

    Article Title: Click-encoded rolling FISH for visualizing single-cell RNA polyadenylation and structures
    Article Snippet: Materials 2-Ethynyl-adenosine (2-EA) and 2-Ethynyl-ATP (2-EATP) were purchased from Jena Bioscience (Jena, Germany).

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  • 93
    Jena Bioscience materials 2 ethynyl adenosine
    Materials 2 Ethynyl Adenosine, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/materials 2 ethynyl adenosine/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    materials 2 ethynyl adenosine - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

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