3 azido 7 hydroxycoumarin (Jena Bioscience)

Jena Bioscience is a verified supplier

Jena Bioscience manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Name:

3 Azido 7 hydroxycoumarin

Description:

Catalog Number:

clk-fa047-1

Molecular Weight:

203.15 g/mol

Price:

105.03

Purity:

> 95 % (HPLC, NMR)

Category:

Click Chemistry

Format:

brown solid

Formula:

C9H5N3O3

Buy from Supplier

Structured Review

Jena Bioscience 3 azido 7 hydroxycoumarin

https://www.bioz.com/result/3 azido 7 hydroxycoumarin/product/Jena Bioscience
Average 93 stars, based on 6 article reviews
Price from $9.99 to $1999.99

3 azido 7 hydroxycoumarin - by Bioz Stars, 2020-07

93/100 stars

Related Products / Commonly Used Together

meoh
pbs
ch3 oh
h2 o
etoh
nacl
dmso
rna
etoac
silica gel column chromatography
acoh
ch2 cl2
icam-1 deficient mice
silybin-phosphatidylcholine
tissue triglyceride content liver tissues

Images

Concentration Assay:

Article Title: Optical manipulation of sphingolipid biosynthesis using photoswitchable ceramides
Article Snippet: .. Click reactions Clickable fluorophores 3-azido-7-hydroxycoumarin (Jena Bioscience) and Alexa-647-azide (Thermo Fisher) were dissolved in CH3 CN to a final concentration of 10 mM and 2 mM, respectively, and stored at −20 ˚C. .. Lipid extracts were transferred to Eppendorf Protein LoBind tubes, dried down in a speedvac and dissolved in 10 μl CHCl3 (initial experiments) or directly dissolved in click mix (later experiments), as indicated.

other:

Article Title: Sphingosine-1-Phosphate Lyase Deficient Cells as a Tool to Study Protein Lipid Interactions
Article Snippet: Lipids were resuspended in 10 μl CHCl3 and 30 μl of a freshly prepared click mix: 2.5 μl of 44.5 mM 3-azido-7-hydroxycoumarin (coumarin azide, Jena Bioscience, CLK-FA047-1), 250 μl of a 10 mM Tetrakis(acetonitrile)copper(I) tetrafluoroborate (Sigma, #677892) in acetonitrile and 1 ml ethanol).

Article Title: Single Particle Automated Raman Trapping Analysis
Article Snippet: Click reaction monitoring by UV-Vis analysis 3-Azido-7-hydroxycoumarin (Jena Bioscience GmbH, Germany) was used to validate whether a CuAAC reaction would occur on the alkyne functionalised PS nanoparticles.

Article Title: Amniotic mesenchymal cells from pre‐eclamptic placentae maintain immunomodulatory features as healthy controls
Article Snippet: Incorporated EdU was detected by the Cu‐catalysed alkyne‐azide cycloaddition (CuAAC or ‘click') reaction of the ethynyl group with 2.5 μM 3‐azido‐7‐hydroxycoumarin (Jena Biosciences, Jena, Germany), in buffer solution (100 mM Tris‐HCl pH 8.0, 10 mM L‐ascorbic acid, 2 mM CuSO4 ) at RT for 30 min.

Article Title: Single Particle Automated Raman Trapping Analysis
Article Snippet: As controls, the measurements were performed at the same time with exclusion of 3-Azido-7-hydroxycoumarin or copper sulphate.

Article Title: The host-cell restriction factor SERINC5 restricts HIV-1 infectivity without altering the lipid composition and organization of viral particles
Article Snippet: Lipids were redissolved in 7 μl of CHCl3 and 30 μl of freshly prepared click mixture containing 3 μl of 44.5 m m 3-azido-7-hydroxycoumarin (Jena Bioscience, CLK-FA047-1) in DMSO, 250 μl of 10 m m Tetrakis(acetonitrile)copper(I) tetrafluoroborate (Sigma-Aldrich, 677892) in MeCN, and 1 ml of EtOH.

Article Title: Sphingosine-1-Phosphate Lyase Deficient Cells as a Tool to Study Protein Lipid Interactions
Article Snippet: 3-azido-7-hydroxycoumarin (coumarin azide CLK-FA047-1) was purchased from Jena Bioscience.

Purification:

Article Title: Single Particle Automated Raman Trapping Analysis
Article Snippet: .. In a 96 well plate 200 µl of a 1000 times dilution of the purified alkyne functionalised PS particles in PBS was combined with 2.88 µl 100 mM sodium ascorbate, 1.80 µl 100 mM copper sulphate and 5 µl 3-Azido-7-hydroxycoumarin 1 μM in water. .. As controls, the measurements were performed at the same time with exclusion of 3-Azido-7-hydroxycoumarin or copper sulphate.