reactions clickable fluorophores 3 azido 7 hydroxycoumarin  (Jena Bioscience)


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    Name:
    3 Azido 7 hydroxycoumarin
    Description:

    Catalog Number:
    clk-fa047-1
    Price:
    110.2
    Category:
    Click Chemistry
    Size:
    1 mg
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    Jena Bioscience reactions clickable fluorophores 3 azido 7 hydroxycoumarin

    https://www.bioz.com/result/reactions clickable fluorophores 3 azido 7 hydroxycoumarin/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    reactions clickable fluorophores 3 azido 7 hydroxycoumarin - by Bioz Stars, 2021-03
    93/100 stars

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    Article Title: Single Particle Automated Raman Trapping Analysis
    Article Snippet: Click reaction monitoring by UV-Vis analysis 3-Azido-7-hydroxycoumarin (Jena Bioscience GmbH, Germany) was used to validate whether a CuAAC reaction would occur on the alkyne functionalised PS nanoparticles.

    Article Title: Amniotic mesenchymal cells from pre‐eclamptic placentae maintain immunomodulatory features as healthy controls
    Article Snippet: Incorporated EdU was detected by the Cu‐catalysed alkyne‐azide cycloaddition (CuAAC or ‘click') reaction of the ethynyl group with 2.5 μM 3‐azido‐7‐hydroxycoumarin (Jena Biosciences, Jena, Germany), in buffer solution (100 mM Tris‐HCl pH 8.0, 10 mM L‐ascorbic acid, 2 mM CuSO4 ) at RT for 30 min.

    Article Title: Single Particle Automated Raman Trapping Analysis
    Article Snippet: As controls, the measurements were performed at the same time with exclusion of 3-Azido-7-hydroxycoumarin or copper sulphate.

    Article Title: CLICK-17, a DNA enzyme that harnesses ultra-low concentrations of either Cu+ or Cu2+ to catalyze the azide-alkyne ‘click’ reaction in water
    Article Snippet: 50 μM 3-azido-7-hydroxycoumarin (‘azide-coumarin’, Jena Bioscience, Germany), from a fresh stock made in dimethylsulfoxide, was then added followed by a mixture of appropriate concentrations of CuSO4 and sodium ascorbate in 10 mM Li-HEPES, pH 7.4 (for the Cu(I) reactions).

    Article Title: The host-cell restriction factor SERINC5 restricts HIV-1 infectivity without altering the lipid composition and organization of viral particles
    Article Snippet: Lipids were redissolved in 7 μl of CHCl3 and 30 μl of freshly prepared click mixture containing 3 μl of 44.5 m m 3-azido-7-hydroxycoumarin (Jena Bioscience, CLK-FA047-1) in DMSO, 250 μl of 10 m m Tetrakis(acetonitrile)copper(I) tetrafluoroborate (Sigma-Aldrich, 677892) in MeCN, and 1 ml of EtOH.

    Article Title: Sphingosine-1-Phosphate Lyase Deficient Cells as a Tool to Study Protein Lipid Interactions
    Article Snippet: 3-azido-7-hydroxycoumarin (coumarin azide CLK-FA047-1) was purchased from Jena Bioscience.

    Purification:

    Article Title: Single Particle Automated Raman Trapping Analysis
    Article Snippet: To do this the fluorescence of the resulting triazole product (Absorption/Emission = 404/477 nm) was monitored by UV-Vis spectroscopy. .. In a 96 well plate 200 µl of a 1000 times dilution of the purified alkyne functionalised PS particles in PBS was combined with 2.88 µl 100 mM sodium ascorbate, 1.80 µl 100 mM copper sulphate and 5 µl 3-Azido-7-hydroxycoumarin 1 μM in water. .. As controls, the measurements were performed at the same time with exclusion of 3-Azido-7-hydroxycoumarin or copper sulphate.

    Concentration Assay:

    Article Title: Optical manipulation of sphingolipid biosynthesis using photoswitchable ceramides
    Article Snippet: After vigorous vortexing for 5 min at RT and subsequent centrifugation (5 min, 21,000 xg , RT), the organic phase (1.8 vol) was collected and used for derivatization with clickable fluorophores. .. Click reactions Clickable fluorophores 3-azido-7-hydroxycoumarin (Jena Bioscience) and Alexa-647-azide (Thermo Fisher) were dissolved in CH3 CN to a final concentration of 10 mM and 2 mM, respectively, and stored at −20 ˚C. .. Lipid extracts were transferred to Eppendorf Protein LoBind tubes, dried down in a speedvac and dissolved in 10 μl CHCl3 (initial experiments) or directly dissolved in click mix (later experiments), as indicated.

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  • 93
    Jena Bioscience reactions clickable fluorophores 3 azido 7 hydroxycoumarin
    Reactions Clickable Fluorophores 3 Azido 7 Hydroxycoumarin, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reactions clickable fluorophores 3 azido 7 hydroxycoumarin/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    reactions clickable fluorophores 3 azido 7 hydroxycoumarin - by Bioz Stars, 2021-03
    93/100 stars
      Buy from Supplier

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