biotin linkers biotin peg3 azide  (Jena Bioscience)


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  • 93
    Name:
    Azide PEG3 Biotin Conjugate
    Description:

    Catalog Number:
    CLK-AZ104P4-100
    Price:
    308.2
    Category:
    Click Chemistry
    Size:
    100 mg
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    Structured Review

    Jena Bioscience biotin linkers biotin peg3 azide

    https://www.bioz.com/result/biotin linkers biotin peg3 azide/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin linkers biotin peg3 azide - by Bioz Stars, 2021-04
    93/100 stars

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    Related Articles

    Isolation:

    Article Title: High-resolution mapping of mitotic DNA synthesis regions and common fragile sites in the human genome through direct sequencing
    Article Snippet: Because a small proportion of the cells had not yet reached G2 phase at the time of the EdU treatment, 2 mM hydroxyurea (Sigma-Aldrich, H8627) was also added to the cells for the last 1.5 h of the RO3306 treatment and for the entire time of the EdU and nocodazole treatment, to suppress the EdU signal from any S phase cells that could potentially detach during the mitotic shake-off. .. Biotinylation and isolation of EdU-labeled DNA was then performed as described previously., Briefly, after fixation, the cells were permeabilized with 0.2% Triton-X in PBS, and EdU was attached to a cleavable biotin linker (Azide-PEG(3 + 3)-S-S-biotin) (Jena Biosciences, Cat. No. CLK-A2112-10) using the reagents of the Click-it Kit (Invitrogen, Cat. No. C-10424). .. The DNA was isolated by phenol-chloroform extraction and ethanol precipitation and then sonicated to a size of 100–500 bp.

    Article Title: High-resolution mapping of mitotic DNA synthesis regions and common fragile sites in the human genome through direct sequencing
    Article Snippet: Two days later, nocodazole (Tocris, Cat. No. 1228) was added to the cells at a concentration of 100 ng/mL for 8 h. Mitotic cells were shaken-off, washed with PBS and released into warm medium with 2 mM hydroxyurea (Sigma, Cat. No. H8627) and 25 µM EdU (Invitrogen Cat. No. A10044). .. EdU-labeled DNA was attached to a cleavable biotin linker (Azide-PEG(3 + 3)-S-S-biotin) (Jena Biosciences, Cat. No. CLK-A2112-10), isolated and subjected to high-throughput sequencing as described above. .. Sequencing libraries were made by the Genomics Platform of the University of Geneva using the TruSeq ChIP Sample Prep Kit (Illumina, Cat. No. IP-202-1012).

    High Throughput Screening Assay:

    Article Title: High-resolution mapping of mitotic DNA synthesis regions and common fragile sites in the human genome through direct sequencing
    Article Snippet: Two days later, nocodazole (Tocris, Cat. No. 1228) was added to the cells at a concentration of 100 ng/mL for 8 h. Mitotic cells were shaken-off, washed with PBS and released into warm medium with 2 mM hydroxyurea (Sigma, Cat. No. H8627) and 25 µM EdU (Invitrogen Cat. No. A10044). .. EdU-labeled DNA was attached to a cleavable biotin linker (Azide-PEG(3 + 3)-S-S-biotin) (Jena Biosciences, Cat. No. CLK-A2112-10), isolated and subjected to high-throughput sequencing as described above. .. Sequencing libraries were made by the Genomics Platform of the University of Geneva using the TruSeq ChIP Sample Prep Kit (Illumina, Cat. No. IP-202-1012).

    Sequencing:

    Article Title: High-resolution mapping of mitotic DNA synthesis regions and common fragile sites in the human genome through direct sequencing
    Article Snippet: Two days later, nocodazole (Tocris, Cat. No. 1228) was added to the cells at a concentration of 100 ng/mL for 8 h. Mitotic cells were shaken-off, washed with PBS and released into warm medium with 2 mM hydroxyurea (Sigma, Cat. No. H8627) and 25 µM EdU (Invitrogen Cat. No. A10044). .. EdU-labeled DNA was attached to a cleavable biotin linker (Azide-PEG(3 + 3)-S-S-biotin) (Jena Biosciences, Cat. No. CLK-A2112-10), isolated and subjected to high-throughput sequencing as described above. .. Sequencing libraries were made by the Genomics Platform of the University of Geneva using the TruSeq ChIP Sample Prep Kit (Illumina, Cat. No. IP-202-1012).

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  • 96
    Jena Bioscience azide peg3 biotin conjugate
    Azide Peg3 Biotin Conjugate, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/azide peg3 biotin conjugate/product/Jena Bioscience
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    azide peg3 biotin conjugate - by Bioz Stars, 2021-04
    96/100 stars
      Buy from Supplier

    93
    Jena Bioscience biotin linkers biotin peg3 azide
    Biotin Linkers Biotin Peg3 Azide, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin linkers biotin peg3 azide/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin linkers biotin peg3 azide - by Bioz Stars, 2021-04
    93/100 stars
      Buy from Supplier

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