enad  (Jena Bioscience)


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    Name:
    2 Ethynyl Adenosine NAD
    Description:

    Catalog Number:
    CLK-043
    Price:
    278.1
    Category:
    Click Chemistry
    Size:
    20 µl
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    Structured Review

    Jena Bioscience enad
    In vitro chemical proteomics analysis suggests that EspJ can <t>ADP-ribosylate</t> multiple tyrosine kinases. Lysates from HeLa (a), A549 (b), polarized Caco2 (c), and differentiated Thp1 (d) cells were incubated with EspJ EHEC and <t>eNAD.</t> ADP-ribosylated proteins were tagged with biotin by click chemistry before enrichment with NeutrAvidin resin and analysis by LFQ MS. Red dots represent proteins that were significantly enriched when abundances in the presence versus the absence of EspJ were compared. Data sets were subjected to a two-sided t test, and black lines display the significance cutoff with an FDR-corrected P value of

    https://www.bioz.com/result/enad/product/Jena Bioscience
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    enad - by Bioz Stars, 2021-06
    92/100 stars

    Images

    1) Product Images from "Broad-Spectrum Regulation of Nonreceptor Tyrosine Kinases by the Bacterial ADP-Ribosyltransferase EspJ"

    Article Title: Broad-Spectrum Regulation of Nonreceptor Tyrosine Kinases by the Bacterial ADP-Ribosyltransferase EspJ

    Journal: mBio

    doi: 10.1128/mBio.00170-18

    In vitro chemical proteomics analysis suggests that EspJ can ADP-ribosylate multiple tyrosine kinases. Lysates from HeLa (a), A549 (b), polarized Caco2 (c), and differentiated Thp1 (d) cells were incubated with EspJ EHEC and eNAD. ADP-ribosylated proteins were tagged with biotin by click chemistry before enrichment with NeutrAvidin resin and analysis by LFQ MS. Red dots represent proteins that were significantly enriched when abundances in the presence versus the absence of EspJ were compared. Data sets were subjected to a two-sided t test, and black lines display the significance cutoff with an FDR-corrected P value of
    Figure Legend Snippet: In vitro chemical proteomics analysis suggests that EspJ can ADP-ribosylate multiple tyrosine kinases. Lysates from HeLa (a), A549 (b), polarized Caco2 (c), and differentiated Thp1 (d) cells were incubated with EspJ EHEC and eNAD. ADP-ribosylated proteins were tagged with biotin by click chemistry before enrichment with NeutrAvidin resin and analysis by LFQ MS. Red dots represent proteins that were significantly enriched when abundances in the presence versus the absence of EspJ were compared. Data sets were subjected to a two-sided t test, and black lines display the significance cutoff with an FDR-corrected P value of

    Techniques Used: In Vitro, Incubation, Mass Spectrometry

    EspJ ADP-ribosylation of NRTKs is dependent on a functional Δ ART domain. Lysate from differentiated Thp1 cells was incubated with EspJ EHEC (WT or D187A mutant) and eNAD. ADP-ribosylated proteins were tagged with biotin by click chemistry before enrichment with NeutrAvidin resin and analysis by LFQ MS. (A) Volcano plot after two-sided t test with a significance cutoff of an FDR-corrected P value of
    Figure Legend Snippet: EspJ ADP-ribosylation of NRTKs is dependent on a functional Δ ART domain. Lysate from differentiated Thp1 cells was incubated with EspJ EHEC (WT or D187A mutant) and eNAD. ADP-ribosylated proteins were tagged with biotin by click chemistry before enrichment with NeutrAvidin resin and analysis by LFQ MS. (A) Volcano plot after two-sided t test with a significance cutoff of an FDR-corrected P value of

    Techniques Used: Functional Assay, Incubation, Mutagenesis, Mass Spectrometry

    2) Product Images from "Broad-Spectrum Regulation of Nonreceptor Tyrosine Kinases by the Bacterial ADP-Ribosyltransferase EspJ"

    Article Title: Broad-Spectrum Regulation of Nonreceptor Tyrosine Kinases by the Bacterial ADP-Ribosyltransferase EspJ

    Journal: mBio

    doi: 10.1128/mBio.00170-18

    In vitro chemical proteomics analysis suggests that EspJ can ADP-ribosylate multiple tyrosine kinases. Lysates from HeLa (a), A549 (b), polarized Caco2 (c), and differentiated Thp1 (d) cells were incubated with EspJ EHEC and eNAD. ADP-ribosylated proteins were tagged with biotin by click chemistry before enrichment with NeutrAvidin resin and analysis by LFQ MS. Red dots represent proteins that were significantly enriched when abundances in the presence versus the absence of EspJ were compared. Data sets were subjected to a two-sided t test, and black lines display the significance cutoff with an FDR-corrected P value of
    Figure Legend Snippet: In vitro chemical proteomics analysis suggests that EspJ can ADP-ribosylate multiple tyrosine kinases. Lysates from HeLa (a), A549 (b), polarized Caco2 (c), and differentiated Thp1 (d) cells were incubated with EspJ EHEC and eNAD. ADP-ribosylated proteins were tagged with biotin by click chemistry before enrichment with NeutrAvidin resin and analysis by LFQ MS. Red dots represent proteins that were significantly enriched when abundances in the presence versus the absence of EspJ were compared. Data sets were subjected to a two-sided t test, and black lines display the significance cutoff with an FDR-corrected P value of

    Techniques Used: In Vitro, Incubation, Mass Spectrometry

    EspJ ADP-ribosylation of NRTKs is dependent on a functional Δ ART domain. Lysate from differentiated Thp1 cells was incubated with EspJ EHEC (WT or D187A mutant) and eNAD. ADP-ribosylated proteins were tagged with biotin by click chemistry before enrichment with NeutrAvidin resin and analysis by LFQ MS. (A) Volcano plot after two-sided t test with a significance cutoff of an FDR-corrected P value of
    Figure Legend Snippet: EspJ ADP-ribosylation of NRTKs is dependent on a functional Δ ART domain. Lysate from differentiated Thp1 cells was incubated with EspJ EHEC (WT or D187A mutant) and eNAD. ADP-ribosylated proteins were tagged with biotin by click chemistry before enrichment with NeutrAvidin resin and analysis by LFQ MS. (A) Volcano plot after two-sided t test with a significance cutoff of an FDR-corrected P value of

    Techniques Used: Functional Assay, Incubation, Mutagenesis, Mass Spectrometry

    Related Articles

    Incubation:

    Article Title: Broad-Spectrum Regulation of Nonreceptor Tyrosine Kinases by the Bacterial ADP-Ribosyltransferase EspJ
    Article Snippet: For testing of Csk inhibition, 1 mM MBP-EspJWT , -EspJD187A , or -TssF1 was incubated with 10 µM His-Csk in ADP-ribosylation buffer. .. After 2 h, glutathione S -transferase (GST)-Src250–533 K295M/Y416A/E310A or poly(Glu4 , Tyr) (Sigma) was added to 1 mM with 10× kinase reaction buffer and the mixture was incubated for 1, 5, 15, or 30 min. For proteomics screening of EspJ targets, 400 μg of HeLa, A549, Caco2, or Thp1 cell lysate in ADP-ribosylation buffer was incubated with 20 μg of MBP-EspJEHEC and eNAD (Jena Biosciences CLK-043) for 3 h at room temperature. .. Click chemistry and pulldown of ADP-ribosylated proteins for LFQ liquid chromatography (LC)-MS.

    Article Title: Broad-Spectrum Regulation of Nonreceptor Tyrosine Kinases by the Bacterial ADP-Ribosyltransferase EspJ
    Article Snippet: For testing of Csk inhibition, 1 mM MBP-EspJWT , -EspJD187A , or -TssF1 was incubated with 10 µM His-Csk in ADP-ribosylation buffer. .. After 2 h, glutathione S -transferase (GST)-Src250–533 K295M/Y416A/E310A or poly(Glu4 , Tyr) (Sigma) was added to 1 mM with 10× kinase reaction buffer and the mixture was incubated for 1, 5, 15, or 30 min. For proteomics screening of EspJ targets, 400 μg of HeLa, A549, Caco2, or Thp1 cell lysate in ADP-ribosylation buffer was incubated with 20 μg of MBP-EspJEHEC and eNAD (Jena Biosciences CLK-043) for 3 h at room temperature. ..

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    Jena Bioscience enad
    In vitro chemical proteomics analysis suggests that EspJ can <t>ADP-ribosylate</t> multiple tyrosine kinases. Lysates from HeLa (a), A549 (b), polarized Caco2 (c), and differentiated Thp1 (d) cells were incubated with EspJ EHEC and <t>eNAD.</t> ADP-ribosylated proteins were tagged with biotin by click chemistry before enrichment with NeutrAvidin resin and analysis by LFQ MS. Red dots represent proteins that were significantly enriched when abundances in the presence versus the absence of EspJ were compared. Data sets were subjected to a two-sided t test, and black lines display the significance cutoff with an FDR-corrected P value of
    Enad, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enad/product/Jena Bioscience
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    enad - by Bioz Stars, 2021-06
    92/100 stars
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    In vitro chemical proteomics analysis suggests that EspJ can ADP-ribosylate multiple tyrosine kinases. Lysates from HeLa (a), A549 (b), polarized Caco2 (c), and differentiated Thp1 (d) cells were incubated with EspJ EHEC and eNAD. ADP-ribosylated proteins were tagged with biotin by click chemistry before enrichment with NeutrAvidin resin and analysis by LFQ MS. Red dots represent proteins that were significantly enriched when abundances in the presence versus the absence of EspJ were compared. Data sets were subjected to a two-sided t test, and black lines display the significance cutoff with an FDR-corrected P value of

    Journal: mBio

    Article Title: Broad-Spectrum Regulation of Nonreceptor Tyrosine Kinases by the Bacterial ADP-Ribosyltransferase EspJ

    doi: 10.1128/mBio.00170-18

    Figure Lengend Snippet: In vitro chemical proteomics analysis suggests that EspJ can ADP-ribosylate multiple tyrosine kinases. Lysates from HeLa (a), A549 (b), polarized Caco2 (c), and differentiated Thp1 (d) cells were incubated with EspJ EHEC and eNAD. ADP-ribosylated proteins were tagged with biotin by click chemistry before enrichment with NeutrAvidin resin and analysis by LFQ MS. Red dots represent proteins that were significantly enriched when abundances in the presence versus the absence of EspJ were compared. Data sets were subjected to a two-sided t test, and black lines display the significance cutoff with an FDR-corrected P value of

    Article Snippet: After 2 h, glutathione S -transferase (GST)-Src250–533 K295M/Y416A/E310A or poly(Glu4 , Tyr) (Sigma) was added to 1 mM with 10× kinase reaction buffer and the mixture was incubated for 1, 5, 15, or 30 min. For proteomics screening of EspJ targets, 400 μg of HeLa, A549, Caco2, or Thp1 cell lysate in ADP-ribosylation buffer was incubated with 20 μg of MBP-EspJEHEC and eNAD (Jena Biosciences CLK-043) for 3 h at room temperature.

    Techniques: In Vitro, Incubation, Mass Spectrometry

    EspJ ADP-ribosylation of NRTKs is dependent on a functional Δ ART domain. Lysate from differentiated Thp1 cells was incubated with EspJ EHEC (WT or D187A mutant) and eNAD. ADP-ribosylated proteins were tagged with biotin by click chemistry before enrichment with NeutrAvidin resin and analysis by LFQ MS. (A) Volcano plot after two-sided t test with a significance cutoff of an FDR-corrected P value of

    Journal: mBio

    Article Title: Broad-Spectrum Regulation of Nonreceptor Tyrosine Kinases by the Bacterial ADP-Ribosyltransferase EspJ

    doi: 10.1128/mBio.00170-18

    Figure Lengend Snippet: EspJ ADP-ribosylation of NRTKs is dependent on a functional Δ ART domain. Lysate from differentiated Thp1 cells was incubated with EspJ EHEC (WT or D187A mutant) and eNAD. ADP-ribosylated proteins were tagged with biotin by click chemistry before enrichment with NeutrAvidin resin and analysis by LFQ MS. (A) Volcano plot after two-sided t test with a significance cutoff of an FDR-corrected P value of

    Article Snippet: After 2 h, glutathione S -transferase (GST)-Src250–533 K295M/Y416A/E310A or poly(Glu4 , Tyr) (Sigma) was added to 1 mM with 10× kinase reaction buffer and the mixture was incubated for 1, 5, 15, or 30 min. For proteomics screening of EspJ targets, 400 μg of HeLa, A549, Caco2, or Thp1 cell lysate in ADP-ribosylation buffer was incubated with 20 μg of MBP-EspJEHEC and eNAD (Jena Biosciences CLK-043) for 3 h at room temperature.

    Techniques: Functional Assay, Incubation, Mutagenesis, Mass Spectrometry

    In vitro chemical proteomics analysis suggests that EspJ can ADP-ribosylate multiple tyrosine kinases. Lysates from HeLa (a), A549 (b), polarized Caco2 (c), and differentiated Thp1 (d) cells were incubated with EspJ EHEC and eNAD. ADP-ribosylated proteins were tagged with biotin by click chemistry before enrichment with NeutrAvidin resin and analysis by LFQ MS. Red dots represent proteins that were significantly enriched when abundances in the presence versus the absence of EspJ were compared. Data sets were subjected to a two-sided t test, and black lines display the significance cutoff with an FDR-corrected P value of

    Journal: mBio

    Article Title: Broad-Spectrum Regulation of Nonreceptor Tyrosine Kinases by the Bacterial ADP-Ribosyltransferase EspJ

    doi: 10.1128/mBio.00170-18

    Figure Lengend Snippet: In vitro chemical proteomics analysis suggests that EspJ can ADP-ribosylate multiple tyrosine kinases. Lysates from HeLa (a), A549 (b), polarized Caco2 (c), and differentiated Thp1 (d) cells were incubated with EspJ EHEC and eNAD. ADP-ribosylated proteins were tagged with biotin by click chemistry before enrichment with NeutrAvidin resin and analysis by LFQ MS. Red dots represent proteins that were significantly enriched when abundances in the presence versus the absence of EspJ were compared. Data sets were subjected to a two-sided t test, and black lines display the significance cutoff with an FDR-corrected P value of

    Article Snippet: After 2 h, glutathione S -transferase (GST)-Src250–533 K295M/Y416A/E310A or poly(Glu4 , Tyr) (Sigma) was added to 1 mM with 10× kinase reaction buffer and the mixture was incubated for 1, 5, 15, or 30 min. For proteomics screening of EspJ targets, 400 μg of HeLa, A549, Caco2, or Thp1 cell lysate in ADP-ribosylation buffer was incubated with 20 μg of MBP-EspJEHEC and eNAD (Jena Biosciences CLK-043) for 3 h at room temperature.

    Techniques: In Vitro, Incubation, Mass Spectrometry

    EspJ ADP-ribosylation of NRTKs is dependent on a functional Δ ART domain. Lysate from differentiated Thp1 cells was incubated with EspJ EHEC (WT or D187A mutant) and eNAD. ADP-ribosylated proteins were tagged with biotin by click chemistry before enrichment with NeutrAvidin resin and analysis by LFQ MS. (A) Volcano plot after two-sided t test with a significance cutoff of an FDR-corrected P value of

    Journal: mBio

    Article Title: Broad-Spectrum Regulation of Nonreceptor Tyrosine Kinases by the Bacterial ADP-Ribosyltransferase EspJ

    doi: 10.1128/mBio.00170-18

    Figure Lengend Snippet: EspJ ADP-ribosylation of NRTKs is dependent on a functional Δ ART domain. Lysate from differentiated Thp1 cells was incubated with EspJ EHEC (WT or D187A mutant) and eNAD. ADP-ribosylated proteins were tagged with biotin by click chemistry before enrichment with NeutrAvidin resin and analysis by LFQ MS. (A) Volcano plot after two-sided t test with a significance cutoff of an FDR-corrected P value of

    Article Snippet: After 2 h, glutathione S -transferase (GST)-Src250–533 K295M/Y416A/E310A or poly(Glu4 , Tyr) (Sigma) was added to 1 mM with 10× kinase reaction buffer and the mixture was incubated for 1, 5, 15, or 30 min. For proteomics screening of EspJ targets, 400 μg of HeLa, A549, Caco2, or Thp1 cell lysate in ADP-ribosylation buffer was incubated with 20 μg of MBP-EspJEHEC and eNAD (Jena Biosciences CLK-043) for 3 h at room temperature.

    Techniques: Functional Assay, Incubation, Mutagenesis, Mass Spectrometry