rabbit polyclonal anti cleaved caspase 3  (Cell Signaling Technology Inc)

Journal: Frontiers in Nutrition

Article Title: Immunomodulatory effects of short-chain fatty acids and immune-supporting nutrients on slice cultures of head and neck tumors

doi: 10.3389/fnut.2026.1731077

Figure Lengend Snippet: Means of CC3 intensity in four different culturing conditions (ns, not significant; ** p < 0.01). Means were compared by Kruskal-Wallis test.

Article Snippet: Immunostaining for cleaved caspase-3 (CC3) was conducted on a Ventana Discovery Ultra immunostainer (Ventana Roche, USA) using a polyclonal rabbit CC3 antibody (diluted at 1:400, #9661, Cell Signalling Technology).

Techniques:

Journal: Frontiers in Nutrition

Article Title: Immunomodulatory effects of short-chain fatty acids and immune-supporting nutrients on slice cultures of head and neck tumors

doi: 10.3389/fnut.2026.1731077

Figure Lengend Snippet: EMM of relative CC3 intensity in 9 patients. Patient 6 and patient 9 (highest intensities) significantly differed from all other patients (Error bars: SEM; ** p < 0.01; *** p < 0.001).

Article Snippet: Immunostaining for cleaved caspase-3 (CC3) was conducted on a Ventana Discovery Ultra immunostainer (Ventana Roche, USA) using a polyclonal rabbit CC3 antibody (diluted at 1:400, #9661, Cell Signalling Technology).

Techniques:

Journal: Frontiers in Nutrition

Article Title: Immunomodulatory effects of short-chain fatty acids and immune-supporting nutrients on slice cultures of head and neck tumors

doi: 10.3389/fnut.2026.1731077

Figure Lengend Snippet: Estimated marginal means (EMMs) of relative CC3 intensity across treatment conditions control, immunonutrition (IN), short-chain fatty acids (SCFAs), and combined IN + SCFAs (Error bars: SEM; ** p < 0.01, and *** p < 0.001).

Article Snippet: Immunostaining for cleaved caspase-3 (CC3) was conducted on a Ventana Discovery Ultra immunostainer (Ventana Roche, USA) using a polyclonal rabbit CC3 antibody (diluted at 1:400, #9661, Cell Signalling Technology).

Techniques: Control

Journal: Frontiers in Nutrition

Article Title: Immunomodulatory effects of short-chain fatty acids and immune-supporting nutrients on slice cultures of head and neck tumors

doi: 10.3389/fnut.2026.1731077

Figure Lengend Snippet: CC3 staining comparing control condition with immunonutrition and immunonutrition combined with SCFAs (blue: CC3 negative cells; brown: CC3 positive cells). (A1) Control condition patient 3; CC3 positive cells in stroma and tumor regions. (A2) Immunonutrition patient 3; lower amount of CC3 positive cells compared to control. (A3) SCFA condition patient 3; CC3 positive cells mostly in tumor regions. (B1) Control condition patient 6; CC3 positive cells in stroma and tumor regions. (B2) Immunonutrition patient 6; lower amount of CC3 positive cells compared to control. (B3) Immunonutrition + SCFAs patient 6; CC3 positive cells mostly in tumor regions (Scale bars: 50 μm).

Article Snippet: Immunostaining for cleaved caspase-3 (CC3) was conducted on a Ventana Discovery Ultra immunostainer (Ventana Roche, USA) using a polyclonal rabbit CC3 antibody (diluted at 1:400, #9661, Cell Signalling Technology).

Techniques: Staining, Control