clear c shaped immuno nonsterile 96 well plates  (Thermo Fisher)


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    Clear C Shaped Immuno Nonsterile 96 Well Plates
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    Perform quantitative and qualitative solid phase immunoassays with Thermo Scientific Clear C Shaped Immuno Nonsterile 96 Well Plates which include Nunc Immulon and Microtiter plates Choose from a variety of binding surfaces for reliable and reproducible results in colormetric assays
    Catalog Number:
    430341
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    Applications:
    Clinical|Diagnostic Development
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    Structured Review

    Thermo Fisher clear c shaped immuno nonsterile 96 well plates
    Perform quantitative and qualitative solid phase immunoassays with Thermo Scientific Clear C Shaped Immuno Nonsterile 96 Well Plates which include Nunc Immulon and Microtiter plates Choose from a variety of binding surfaces for reliable and reproducible results in colormetric assays
    https://www.bioz.com/result/clear c shaped immuno nonsterile 96 well plates/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    clear c shaped immuno nonsterile 96 well plates - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: The prophylactic effects of human IgG derived from sera containing high anti-PcrV titers against pneumonia-causing Pseudomonas aeruginosa
    Article Snippet: Quantification of the anti-PcrV titers was performed using an enzyme-linked immunosorbent assay (ELISA). .. Micro-well plates (Nunc C96 Maxisorp, Thermo Fisher Scientific Inc., #430341) were coated for 2 h at 4°C with recombinant PcrV suspended in coating buffer (1.5 μg/mL in coating solution, 0.05M NaHCO3 , pH 9.6).

    Article Title: Single-cell reconstruction of follicular remodeling in the human adult ovary
    Article Snippet: Paragraph title: C1Q and C3 ELISA on human ovarian tissue ... Briefly, Nunc Maxisorp plates (430341, Thermo Scientific) were either coated with a solution (1 µg/ml) of mouse anti-human C1Q or rabbit anti-human anti-C3c (A0062, DAKO) diluted in coating buffer (0.1 M Na2 CO3 , 0.1 M NaHCO3 ) overnight at RT.

    Article Title: An abundant dysfunctional apolipoprotein A1 in human atheroma
    Article Snippet: .. Indirect ELISA Nunc MaxSorp ELISA plates (#446612, Thermo Fisher Scientific, Waltham, MA) were coated with the indicated purified native or oxidized protein (0.2 μg ml−1 ) in Carbonate and Bicarbonate buffer, pH 9.6. .. Purified human apoA1, rh-apoA1 and mutant apoA1’s were modified by either the MPO/H2 O2 /Cl− system, the MPO/H2 O2 /NO2 − system or by multiple other oxidation systems as indicated at 10:1 molar ratio of oxidants/apoA1, unless otherwise specified, details of oxidation conditions are described in reference .

    Article Title: An abundant dysfunctional apolipoprotein A1 in human atheroma
    Article Snippet: .. Competitive ELISA Nunc MaxSorp ELISA plates (#446612, Thermo Fisher Scientific, Waltham, MA) were coated with 0.5 μg ml−1 Cl.apoA1 in Carbonate and Biocarbonate buffer, pH 9.6. (human apoA1 modified by MPO/H2 O2 /Cl− complete oxidative system at 10:1 molar ratio of H2 O2 /apoA1). r8B5.2 (25 ng ml−1 ) and peptides were added into the 5% BSA blocked ELISA wells and incubated for 1 hr at room temperature. .. The signal was detected by Peroxidase AffiniPure Goat Anti-Mouse IgG, F(ab’)2 Fragment Specific (1:10,000 dilution, Code # 115-036-006, Jackson ImmunoResearch Laboratories, Inc., West Grove, PA).

    Mutagenesis:

    Article Title: An abundant dysfunctional apolipoprotein A1 in human atheroma
    Article Snippet: Indirect ELISA Nunc MaxSorp ELISA plates (#446612, Thermo Fisher Scientific, Waltham, MA) were coated with the indicated purified native or oxidized protein (0.2 μg ml−1 ) in Carbonate and Bicarbonate buffer, pH 9.6. .. Purified human apoA1, rh-apoA1 and mutant apoA1’s were modified by either the MPO/H2 O2 /Cl− system, the MPO/H2 O2 /NO2 − system or by multiple other oxidation systems as indicated at 10:1 molar ratio of oxidants/apoA1, unless otherwise specified, details of oxidation conditions are described in reference .

    Cell Culture:

    Article Title: Single-cell reconstruction of follicular remodeling in the human adult ovary
    Article Snippet: C1Q and C3 ELISA on human ovarian tissue Small pieces (2 × 2 × 2 mm) of ovarian stroma (N = 3) and atretic follicle walls (N = 6) from one human adult ovary were cultured individually in 96-wells plates (655180, Cellstar) with 120 µl McCoy’s 5A (Modified) Medium (22330-021, Life Technologies) supplemented with 5% fetal bovine serum (FBS, FB1001, Biosera), l -Glutamine (2 mM, 25030081, Thermo Scientific) and Penicillin–Streptomycin (50 U/ml, 15070063, Thermo Scientific). .. Briefly, Nunc Maxisorp plates (430341, Thermo Scientific) were either coated with a solution (1 µg/ml) of mouse anti-human C1Q or rabbit anti-human anti-C3c (A0062, DAKO) diluted in coating buffer (0.1 M Na2 CO3 , 0.1 M NaHCO3 ) overnight at RT.

    Purification:

    Article Title: Engineering and Application of LOV2-based Photoswitches
    Article Snippet: .. 96-well Maxisorp immunoplates (Nunc, cat. no. 430341) M13K07 helper phage (Invitrogen) SS320 E. coli cells (Invitrogen) BSA (Sigma, cat. No. A3059) Sodium Bicarbonate buffer pH 9.6 Purified target protein 2YT media PBS-Tween20 0.1% (PBS-T) ..

    Article Title: An abundant dysfunctional apolipoprotein A1 in human atheroma
    Article Snippet: .. Indirect ELISA Nunc MaxSorp ELISA plates (#446612, Thermo Fisher Scientific, Waltham, MA) were coated with the indicated purified native or oxidized protein (0.2 μg ml−1 ) in Carbonate and Bicarbonate buffer, pH 9.6. .. Purified human apoA1, rh-apoA1 and mutant apoA1’s were modified by either the MPO/H2 O2 /Cl− system, the MPO/H2 O2 /NO2 − system or by multiple other oxidation systems as indicated at 10:1 molar ratio of oxidants/apoA1, unless otherwise specified, details of oxidation conditions are described in reference .

    Indirect ELISA:

    Article Title: An abundant dysfunctional apolipoprotein A1 in human atheroma
    Article Snippet: .. Indirect ELISA Nunc MaxSorp ELISA plates (#446612, Thermo Fisher Scientific, Waltham, MA) were coated with the indicated purified native or oxidized protein (0.2 μg ml−1 ) in Carbonate and Bicarbonate buffer, pH 9.6. .. Purified human apoA1, rh-apoA1 and mutant apoA1’s were modified by either the MPO/H2 O2 /Cl− system, the MPO/H2 O2 /NO2 − system or by multiple other oxidation systems as indicated at 10:1 molar ratio of oxidants/apoA1, unless otherwise specified, details of oxidation conditions are described in reference .

    Concentration Assay:

    Article Title: Single-cell reconstruction of follicular remodeling in the human adult ovary
    Article Snippet: The concentration of C1Q and C3 in the culture medium was determined by an in house-made ELISA . .. Briefly, Nunc Maxisorp plates (430341, Thermo Scientific) were either coated with a solution (1 µg/ml) of mouse anti-human C1Q or rabbit anti-human anti-C3c (A0062, DAKO) diluted in coating buffer (0.1 M Na2 CO3 , 0.1 M NaHCO3 ) overnight at RT.

    Incubation:

    Article Title: Single-cell reconstruction of follicular remodeling in the human adult ovary
    Article Snippet: Briefly, Nunc Maxisorp plates (430341, Thermo Scientific) were either coated with a solution (1 µg/ml) of mouse anti-human C1Q or rabbit anti-human anti-C3c (A0062, DAKO) diluted in coating buffer (0.1 M Na2 CO3 , 0.1 M NaHCO3 ) overnight at RT. .. After three times washing with 0.05% Tween-20 in PBS, the plates were incubated with either rabbit anti-human C1q (1:1000, A0136, DAKO) or goat anti-human C3 (1:5000, A213, Complement technology), followed by goat anti-rabbit Ig-HRP (1:5000, P0448, DAKO), or rabbit anti-goat Ig-HRP (1:5000, P0449, DAKO), respectively, all diluted in PBT buffer.

    Article Title: An abundant dysfunctional apolipoprotein A1 in human atheroma
    Article Snippet: Indirect ELISA Nunc MaxSorp ELISA plates (#446612, Thermo Fisher Scientific, Waltham, MA) were coated with the indicated purified native or oxidized protein (0.2 μg ml−1 ) in Carbonate and Bicarbonate buffer, pH 9.6. .. Primary antibody r8B5.2 (50 ng ml−1 ) ( and ) was added into the 5% BSA blocked ELISA wells and incubated for 1 hr at room temperature.

    Article Title: An abundant dysfunctional apolipoprotein A1 in human atheroma
    Article Snippet: .. Competitive ELISA Nunc MaxSorp ELISA plates (#446612, Thermo Fisher Scientific, Waltham, MA) were coated with 0.5 μg ml−1 Cl.apoA1 in Carbonate and Biocarbonate buffer, pH 9.6. (human apoA1 modified by MPO/H2 O2 /Cl− complete oxidative system at 10:1 molar ratio of H2 O2 /apoA1). r8B5.2 (25 ng ml−1 ) and peptides were added into the 5% BSA blocked ELISA wells and incubated for 1 hr at room temperature. .. The signal was detected by Peroxidase AffiniPure Goat Anti-Mouse IgG, F(ab’)2 Fragment Specific (1:10,000 dilution, Code # 115-036-006, Jackson ImmunoResearch Laboratories, Inc., West Grove, PA).

    Activity Assay:

    Article Title: Single-cell reconstruction of follicular remodeling in the human adult ovary
    Article Snippet: Briefly, Nunc Maxisorp plates (430341, Thermo Scientific) were either coated with a solution (1 µg/ml) of mouse anti-human C1Q or rabbit anti-human anti-C3c (A0062, DAKO) diluted in coating buffer (0.1 M Na2 CO3 , 0.1 M NaHCO3 ) overnight at RT. .. The enzymatic activity of HRP was measured after incubation with ABTS (A1888-5G, Merck) and H2 O2 (1072090250, Merck) at absorbance 415 nm on a microplate reader (BioRad).

    Blocking Assay:

    Article Title: Single-cell reconstruction of follicular remodeling in the human adult ovary
    Article Snippet: Briefly, Nunc Maxisorp plates (430341, Thermo Scientific) were either coated with a solution (1 µg/ml) of mouse anti-human C1Q or rabbit anti-human anti-C3c (A0062, DAKO) diluted in coating buffer (0.1 M Na2 CO3 , 0.1 M NaHCO3 ) overnight at RT. .. After three times washing with 0.05% Tween-20 in PBS and blocking with 1% BSA in PBS for 1 h at 37 °C, the culture media diluted in PBT buffer (1% BSA, 0.05% Tween-20 in PBS) was added (1:1 dilution for C1Q and 1:12 dilution for C3) to the plates for 1 h at 37 °C.

    Modification:

    Article Title: Single-cell reconstruction of follicular remodeling in the human adult ovary
    Article Snippet: C1Q and C3 ELISA on human ovarian tissue Small pieces (2 × 2 × 2 mm) of ovarian stroma (N = 3) and atretic follicle walls (N = 6) from one human adult ovary were cultured individually in 96-wells plates (655180, Cellstar) with 120 µl McCoy’s 5A (Modified) Medium (22330-021, Life Technologies) supplemented with 5% fetal bovine serum (FBS, FB1001, Biosera), l -Glutamine (2 mM, 25030081, Thermo Scientific) and Penicillin–Streptomycin (50 U/ml, 15070063, Thermo Scientific). .. Briefly, Nunc Maxisorp plates (430341, Thermo Scientific) were either coated with a solution (1 µg/ml) of mouse anti-human C1Q or rabbit anti-human anti-C3c (A0062, DAKO) diluted in coating buffer (0.1 M Na2 CO3 , 0.1 M NaHCO3 ) overnight at RT.

    Article Title: An abundant dysfunctional apolipoprotein A1 in human atheroma
    Article Snippet: Indirect ELISA Nunc MaxSorp ELISA plates (#446612, Thermo Fisher Scientific, Waltham, MA) were coated with the indicated purified native or oxidized protein (0.2 μg ml−1 ) in Carbonate and Bicarbonate buffer, pH 9.6. .. Purified human apoA1, rh-apoA1 and mutant apoA1’s were modified by either the MPO/H2 O2 /Cl− system, the MPO/H2 O2 /NO2 − system or by multiple other oxidation systems as indicated at 10:1 molar ratio of oxidants/apoA1, unless otherwise specified, details of oxidation conditions are described in reference .

    Article Title: An abundant dysfunctional apolipoprotein A1 in human atheroma
    Article Snippet: .. Competitive ELISA Nunc MaxSorp ELISA plates (#446612, Thermo Fisher Scientific, Waltham, MA) were coated with 0.5 μg ml−1 Cl.apoA1 in Carbonate and Biocarbonate buffer, pH 9.6. (human apoA1 modified by MPO/H2 O2 /Cl− complete oxidative system at 10:1 molar ratio of H2 O2 /apoA1). r8B5.2 (25 ng ml−1 ) and peptides were added into the 5% BSA blocked ELISA wells and incubated for 1 hr at room temperature. .. The signal was detected by Peroxidase AffiniPure Goat Anti-Mouse IgG, F(ab’)2 Fragment Specific (1:10,000 dilution, Code # 115-036-006, Jackson ImmunoResearch Laboratories, Inc., West Grove, PA).

    Competitive ELISA:

    Article Title: An abundant dysfunctional apolipoprotein A1 in human atheroma
    Article Snippet: .. Competitive ELISA Nunc MaxSorp ELISA plates (#446612, Thermo Fisher Scientific, Waltham, MA) were coated with 0.5 μg ml−1 Cl.apoA1 in Carbonate and Biocarbonate buffer, pH 9.6. (human apoA1 modified by MPO/H2 O2 /Cl− complete oxidative system at 10:1 molar ratio of H2 O2 /apoA1). r8B5.2 (25 ng ml−1 ) and peptides were added into the 5% BSA blocked ELISA wells and incubated for 1 hr at room temperature. .. The signal was detected by Peroxidase AffiniPure Goat Anti-Mouse IgG, F(ab’)2 Fragment Specific (1:10,000 dilution, Code # 115-036-006, Jackson ImmunoResearch Laboratories, Inc., West Grove, PA).

    Recombinant:

    Article Title: The prophylactic effects of human IgG derived from sera containing high anti-PcrV titers against pneumonia-causing Pseudomonas aeruginosa
    Article Snippet: .. Micro-well plates (Nunc C96 Maxisorp, Thermo Fisher Scientific Inc., #430341) were coated for 2 h at 4°C with recombinant PcrV suspended in coating buffer (1.5 μg/mL in coating solution, 0.05M NaHCO3 , pH 9.6). .. Plates were washed twice with phosphate-buffered saline (PBS) with 0.05% Tween-20 (Sigma-Aldrich Co., P9416), and then blocked with 200 μL of 1% bovine serum albumin/PBS overnight at 4°C.

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  • 99
    Thermo Fisher clear c shaped immuno nonsterile 96 well plates
    Clear C Shaped Immuno Nonsterile 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/clear c shaped immuno nonsterile 96 well plates/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    clear c shaped immuno nonsterile 96 well plates - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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