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GE Healthcare ci mmol
Ci Mmol, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ci mmol/product/GE Healthcare
Average 94 stars, based on 57 article reviews
Price from $9.99 to $1999.99
ci mmol - by Bioz Stars, 2020-04
94/100 stars

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Related Articles

Clone Assay:

Article Title: Electrophoretic Mobility Shift Assays for Protein–DNA Complexes Involved in DNA Repair *
Article Snippet: DDB1 plasmid: cloned into pCDNA3 vector (Invitrogen, Carlsbad, CA). .. 35 S-labeled methionine: 1,000 Ci/mmol, 50 mCi/mL (GE Healthcare Life Sciences, Piscataway, NJ).

Centrifugation:

Article Title: Deletion of the PAT1 Gene Affects Translation Initiation and Suppresses a PAB1 Gene Deletion in Yeast
Article Snippet: Polysomes were pelleted by centrifugation through a 20% sucrose pad at 100,000 × g for 30 min. RNA was purified from the pellet by phenol extraction. .. To measure the amounts of poly(A)- and cap-containing RNA, 1 ml of yeast culture was labeled for 5 min with 5 μCi of [8-3 H]adenine at a concentration of 24 Ci/mmol (Amersham).

Positive Control:

Article Title: Identification of a Dual-Specific T Cell Epitope of the Hemagglutinin Antigen of an H5 Avian Influenza Virus in Chickens
Article Snippet: Spleen cells were also treated with concanavalin A (ConA) (Sigma Aldrich, St. Louis, Mo) at 20 µg/ml (positive control), or with peptide solvent or medium as negative controls. .. Eighteen hours before the end of each timepoint, 1 µCi of methyl-3H-thymidine, 25 Ci/mmol (GE Healthcare, Baie d'Urfe, Quebec) was added to each well.

Synthesized:

Article Title: The Quorum-Sensing Molecule Farnesol Is a Modulator of Drug Efflux Mediated by ABC Multidrug Transporters and Synergizes with Drugs in Candida albicans ▿ ▿ †
Article Snippet: .. [3 H]fluconazole ([3 H]FLC; specific activity, 19 Ci/mmol) was custom synthesized from Amersham Biosciences, United Kingdom, and [3 H]methotrexate ([3 H]MTX; specific activity, 8.60 Ci/mmol) was procured from Amersham Biosciences, United Kingdom. .. 2′,7′-Dichlorofluorescin diacetate (DCFH-DA), ascorbic acid (AA), and other molecular-grade chemicals were obtained from Sigma Chemicals Co. (St. Louis, MO).

Article Title: A new pathway for vacuolar cadmium sequestration in Saccharomyces cerevisiae: YCF1-catalyzed transport of bis(glutathionato)cadmium
Article Snippet: [3 H]GSH [( glycine -2-3 H)- l -Glu-Cys-Gly; 4.4 Ci/mmol] was from DuPont/NEN, and 109 CdSO4 (78.44 Ci/mmol) was from Amersham. .. All other reagents were of analytical grade and purchased from Fisher, Fluka, Research Organics, or Sigma or synthesized as described ( ).

Article Title: Comparative metabolism of benzo[a]pyrene by human keratinocytes infected with high-risk human papillomavirus types 16 and 18 as episomal or integrated genomes
Article Snippet: [3 H]B[a ]P, specific activity = 83.0 Ci/mmol, was obtained from Amersham Life Science, Buckinghamshire, England. .. The following chemicals were purchased from the National Cancer Institute's Chemical Carcinogen Reference Standard Repository at the Midwest Research Institute, Kansas City, MO, USA: trans -4,5-dihydroxy-4,5-dihydro-benzo[a]pyrene (B[a ]P-4,5-diol), B[a ]P-7,8-diol, B[a ]P-9,10-diol and 3-OH-B[a ]P. trans,anti -B[a ]P-tetraol was synthesized as previously described.

Plasmid Purification:

Article Title: Ability of Thermophilic Lactic Acid Bacteria To Produce Aroma Compounds from Amino Acids
Article Snippet: The basic reaction mixture consisted of 70 mM potassium phosphate buffer (pH 5.5), 3 mM unlabeled amino acid (Phe, Leu, or Met), and 0.05 μM radiolabeled amino acid ( l -[2,3,4,5,6-3 H]phenylalanine, 100 to 140 Ci/mmol; l -[4,5-3 H]leucine, 120 to 190 Ci/mmol; or l -[methyl-3 H]methionine, 70 to 85 Ci/mmol) (Amersham Pharmacia Biotech). .. In some experiments, 0.05 mM pyridoxal phosphate (PLP) and 10 mM α-ketoglutarate (α-KG) or 10 mM glutamate (Glu) were added to the reaction medium.

Incubation:

Article Title: Multiple Roles of Myd88 in the Immune Response to the Plague F1-V Vaccine and in Protection against an Aerosol Challenge of Yersinia pestis CO92 in Mice
Article Snippet: .. Incubation was continued for 18–24 h with 1 μ Ci of [3H] thymidine at a specific activity of 5 Ci/mmol (Amersham Life Sciences, Arlington, IL) before collecting the cells and counting the amount of radioactivity incorporated. .. Histochemical/Immunohistochemical Analysis of Spleens Spleens of mice in the Myd88 -deficient vaccine study were removed as soon as we found the nonsurviving mouse early in the morning or through the work day up to 21 days after challenge.

Article Title: Ability of Thermophilic Lactic Acid Bacteria To Produce Aroma Compounds from Amino Acids
Article Snippet: The basic reaction mixture consisted of 70 mM potassium phosphate buffer (pH 5.5), 3 mM unlabeled amino acid (Phe, Leu, or Met), and 0.05 μM radiolabeled amino acid ( l -[2,3,4,5,6-3 H]phenylalanine, 100 to 140 Ci/mmol; l -[4,5-3 H]leucine, 120 to 190 Ci/mmol; or l -[methyl-3 H]methionine, 70 to 85 Ci/mmol) (Amersham Pharmacia Biotech). .. Resting cell suspension (0.05 ml) was added to 0.45 ml of the different reaction mixtures, and the mixtures were incubated for 40 h at 37°C.

Activity Assay:

Article Title: The Quorum-Sensing Molecule Farnesol Is a Modulator of Drug Efflux Mediated by ABC Multidrug Transporters and Synergizes with Drugs in Candida albicans ▿ ▿ †
Article Snippet: .. [3 H]fluconazole ([3 H]FLC; specific activity, 19 Ci/mmol) was custom synthesized from Amersham Biosciences, United Kingdom, and [3 H]methotrexate ([3 H]MTX; specific activity, 8.60 Ci/mmol) was procured from Amersham Biosciences, United Kingdom. .. 2′,7′-Dichlorofluorescin diacetate (DCFH-DA), ascorbic acid (AA), and other molecular-grade chemicals were obtained from Sigma Chemicals Co. (St. Louis, MO).

Article Title: Interaction between complement receptor gC1qR and hepatitis C virus core protein inhibits T-lymphocyte proliferation
Article Snippet: .. At the 60-hour time point, 1 μCi/well of 3 (H)-thymidine was added (specific activity of 6.7 Ci/mmol; Amersham International, Oakville, Ontario, Canada). ..

Article Title: Detoxification of aristolochic acid I by O-demethylation: Less nephrotoxicity and genotoxicity of aristolochic acid la in rodents
Article Snippet: .. [γ-32 P]-ATP specific activity, > 6,000 Ci/mmol) was obtained from GE Healthcare Bio-sciences (Piscataway, NJ). .. Pure AA I was separated from a mixture of AA I and AA II (Fisher Scientific, Fairlawn, NJ) using preparative reverse-phase HPLC on a Varian automated ProStar System (Palo Alto, CA), as described previously.

Article Title: ADP-Ribosylation Factors Modulate the Cell Surface Transport of G Protein-Coupled Receptors S⃞
Article Snippet: .. [3 H]CGP-12177 (specific activity = 51 Ci/mmol), [3 H]RX 821002 (41 Ci/mmol), l -[ N -methyl-3 H]scopolamine methyl chloride ([3 H]NMS, 80 Ci/mmol), and (3-(125 I)-iodotyrosyl 4) Ang II (125 I-Ang II) (2000 Ci/mmol) were purchased from GE Healthcare (Piscataway, NJ). .. Alexa Fluor 594-labeled secondary antibodies and 4,6-diamidino-2-phenylindole were from Invitrogen.

Article Title: Multiple Roles of Myd88 in the Immune Response to the Plague F1-V Vaccine and in Protection against an Aerosol Challenge of Yersinia pestis CO92 in Mice
Article Snippet: .. Incubation was continued for 18–24 h with 1 μ Ci of [3H] thymidine at a specific activity of 5 Ci/mmol (Amersham Life Sciences, Arlington, IL) before collecting the cells and counting the amount of radioactivity incorporated. .. Histochemical/Immunohistochemical Analysis of Spleens Spleens of mice in the Myd88 -deficient vaccine study were removed as soon as we found the nonsurviving mouse early in the morning or through the work day up to 21 days after challenge.

Article Title: Comparative metabolism of benzo[a]pyrene by human keratinocytes infected with high-risk human papillomavirus types 16 and 18 as episomal or integrated genomes
Article Snippet: .. [3 H]B[a ]P, specific activity = 83.0 Ci/mmol, was obtained from Amersham Life Science, Buckinghamshire, England. .. The following chemicals were purchased from the National Cancer Institute's Chemical Carcinogen Reference Standard Repository at the Midwest Research Institute, Kansas City, MO, USA: trans -4,5-dihydroxy-4,5-dihydro-benzo[a]pyrene (B[a ]P-4,5-diol), B[a ]P-7,8-diol, B[a ]P-9,10-diol and 3-OH-B[a ]P. trans,anti -B[a ]P-tetraol was synthesized as previously described.

High Performance Liquid Chromatography:

Article Title: Detoxification of aristolochic acid I by O-demethylation: Less nephrotoxicity and genotoxicity of aristolochic acid la in rodents
Article Snippet: [γ-32 P]-ATP specific activity, > 6,000 Ci/mmol) was obtained from GE Healthcare Bio-sciences (Piscataway, NJ). .. Pure AA I was separated from a mixture of AA I and AA II (Fisher Scientific, Fairlawn, NJ) using preparative reverse-phase HPLC on a Varian automated ProStar System (Palo Alto, CA), as described previously.

Article Title: Ability of Thermophilic Lactic Acid Bacteria To Produce Aroma Compounds from Amino Acids
Article Snippet: Paragraph title: Analysis of amino acid catabolism by HPLC. ... The basic reaction mixture consisted of 70 mM potassium phosphate buffer (pH 5.5), 3 mM unlabeled amino acid (Phe, Leu, or Met), and 0.05 μM radiolabeled amino acid ( l -[2,3,4,5,6-3 H]phenylalanine, 100 to 140 Ci/mmol; l -[4,5-3 H]leucine, 120 to 190 Ci/mmol; or l -[methyl-3 H]methionine, 70 to 85 Ci/mmol) (Amersham Pharmacia Biotech).

Chromatography:

Article Title: Deletion of the PAT1 Gene Affects Translation Initiation and Suppresses a PAB1 Gene Deletion in Yeast
Article Snippet: To measure the amounts of poly(A)- and cap-containing RNA, 1 ml of yeast culture was labeled for 5 min with 5 μCi of [8-3 H]adenine at a concentration of 24 Ci/mmol (Amersham). .. Poly(A)+ RNA was purified by batch chromatography on oligo(dT) cellulose ( ).

Concentration Assay:

Article Title: Identification of a Dual-Specific T Cell Epitope of the Hemagglutinin Antigen of an H5 Avian Influenza Virus in Chickens
Article Snippet: Cells were suspended in cell culture medium and peptide pools were added to a final concentration of 1.0 or 10 µM (per peptide). .. Eighteen hours before the end of each timepoint, 1 µCi of methyl-3H-thymidine, 25 Ci/mmol (GE Healthcare, Baie d'Urfe, Quebec) was added to each well.

Article Title: Deletion of the PAT1 Gene Affects Translation Initiation and Suppresses a PAB1 Gene Deletion in Yeast
Article Snippet: .. To measure the amounts of poly(A)- and cap-containing RNA, 1 ml of yeast culture was labeled for 5 min with 5 μCi of [8-3 H]adenine at a concentration of 24 Ci/mmol (Amersham). .. Poly(A)+ RNA was purified by batch chromatography on oligo(dT) cellulose ( ).

Northern Blot:

Article Title: Deletion of the PAT1 Gene Affects Translation Initiation and Suppresses a PAB1 Gene Deletion in Yeast
Article Snippet: Total yeast RNA was isolated and mRNAs were analyzed on Northern blots by standard methods ( ). .. To measure the amounts of poly(A)- and cap-containing RNA, 1 ml of yeast culture was labeled for 5 min with 5 μCi of [8-3 H]adenine at a concentration of 24 Ci/mmol (Amersham).

Cell Culture:

Article Title: Identification of a Dual-Specific T Cell Epitope of the Hemagglutinin Antigen of an H5 Avian Influenza Virus in Chickens
Article Snippet: Cells were suspended in cell culture medium and peptide pools were added to a final concentration of 1.0 or 10 µM (per peptide). .. Eighteen hours before the end of each timepoint, 1 µCi of methyl-3H-thymidine, 25 Ci/mmol (GE Healthcare, Baie d'Urfe, Quebec) was added to each well.

Recombinant:

Article Title: Interaction between complement receptor gC1qR and hepatitis C virus core protein inhibits T-lymphocyte proliferation
Article Snippet: Recombinant HCV core and NS3 proteins were purified from bacteria purchased from ViroGen (Watertown, Massachusetts, USA). .. At the 60-hour time point, 1 μCi/well of 3 (H)-thymidine was added (specific activity of 6.7 Ci/mmol; Amersham International, Oakville, Ontario, Canada).

MTT Assay:

Article Title: The Quorum-Sensing Molecule Farnesol Is a Modulator of Drug Efflux Mediated by ABC Multidrug Transporters and Synergizes with Drugs in Candida albicans ▿ ▿ †
Article Snippet: Rhodamine 6G (R6G), 2,4-dinitrophenol (DNP), 2-deoxy- d -glucose (DOG), oligomycin, 3-(4,5-dimethyl thiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT), Nile red (NR), and other molecular-grade chemicals were obtained from Sigma Chemicals Co. (St. Louis, MO). .. [3 H]fluconazole ([3 H]FLC; specific activity, 19 Ci/mmol) was custom synthesized from Amersham Biosciences, United Kingdom, and [3 H]methotrexate ([3 H]MTX; specific activity, 8.60 Ci/mmol) was procured from Amersham Biosciences, United Kingdom.

Radioactivity:

Article Title: Multiple Roles of Myd88 in the Immune Response to the Plague F1-V Vaccine and in Protection against an Aerosol Challenge of Yersinia pestis CO92 in Mice
Article Snippet: .. Incubation was continued for 18–24 h with 1 μ Ci of [3H] thymidine at a specific activity of 5 Ci/mmol (Amersham Life Sciences, Arlington, IL) before collecting the cells and counting the amount of radioactivity incorporated. .. Histochemical/Immunohistochemical Analysis of Spleens Spleens of mice in the Myd88 -deficient vaccine study were removed as soon as we found the nonsurviving mouse early in the morning or through the work day up to 21 days after challenge.

Isolation:

Article Title: Deletion of the PAT1 Gene Affects Translation Initiation and Suppresses a PAB1 Gene Deletion in Yeast
Article Snippet: Paragraph title: RNA isolation, poly(A) and cap selection, and analysis of mRNA poly(A) tail lengths. ... To measure the amounts of poly(A)- and cap-containing RNA, 1 ml of yeast culture was labeled for 5 min with 5 μCi of [8-3 H]adenine at a concentration of 24 Ci/mmol (Amersham).

Labeling:

Article Title: Deletion of the PAT1 Gene Affects Translation Initiation and Suppresses a PAB1 Gene Deletion in Yeast
Article Snippet: .. To measure the amounts of poly(A)- and cap-containing RNA, 1 ml of yeast culture was labeled for 5 min with 5 μCi of [8-3 H]adenine at a concentration of 24 Ci/mmol (Amersham). .. Poly(A)+ RNA was purified by batch chromatography on oligo(dT) cellulose ( ).

Purification:

Article Title: Interaction between complement receptor gC1qR and hepatitis C virus core protein inhibits T-lymphocyte proliferation
Article Snippet: Recombinant HCV core and NS3 proteins were purified from bacteria purchased from ViroGen (Watertown, Massachusetts, USA). .. At the 60-hour time point, 1 μCi/well of 3 (H)-thymidine was added (specific activity of 6.7 Ci/mmol; Amersham International, Oakville, Ontario, Canada).

Article Title: Deletion of the PAT1 Gene Affects Translation Initiation and Suppresses a PAB1 Gene Deletion in Yeast
Article Snippet: Polysomes were pelleted by centrifugation through a 20% sucrose pad at 100,000 × g for 30 min. RNA was purified from the pellet by phenol extraction. .. To measure the amounts of poly(A)- and cap-containing RNA, 1 ml of yeast culture was labeled for 5 min with 5 μCi of [8-3 H]adenine at a concentration of 24 Ci/mmol (Amersham).

Article Title: Lobeline effects on tonic and methamphetamine-induced dopamine release
Article Snippet: [3 H]dopamine (3,4-[7-3 H]dihydroxyphenylethylamine, 5.8–9.7 Ci/mmol) was purchased from Amersham Biosciences (Piscataway, NJ, U.S.A.). .. All water used in these experiments was purified by a Milli-Q system (Millipore Corp., Bedford, MA, U.S.A.).

Plasmid Preparation:

Article Title: Electrophoretic Mobility Shift Assays for Protein–DNA Complexes Involved in DNA Repair *
Article Snippet: DDB1 plasmid: cloned into pCDNA3 vector (Invitrogen, Carlsbad, CA). .. 35 S-labeled methionine: 1,000 Ci/mmol, 50 mCi/mL (GE Healthcare Life Sciences, Piscataway, NJ).

Selection:

Article Title: Deletion of the PAT1 Gene Affects Translation Initiation and Suppresses a PAB1 Gene Deletion in Yeast
Article Snippet: Paragraph title: RNA isolation, poly(A) and cap selection, and analysis of mRNA poly(A) tail lengths. ... To measure the amounts of poly(A)- and cap-containing RNA, 1 ml of yeast culture was labeled for 5 min with 5 μCi of [8-3 H]adenine at a concentration of 24 Ci/mmol (Amersham).

In Vitro:

Article Title: Identification of a Dual-Specific T Cell Epitope of the Hemagglutinin Antigen of an H5 Avian Influenza Virus in Chickens
Article Snippet: Paragraph title: In Vitro Stimulation of Spleen Cells Using Peptide Pools and Individual Peptides ... Eighteen hours before the end of each timepoint, 1 µCi of methyl-3H-thymidine, 25 Ci/mmol (GE Healthcare, Baie d'Urfe, Quebec) was added to each well.

Article Title: Electrophoretic Mobility Shift Assays for Protein–DNA Complexes Involved in DNA Repair *
Article Snippet: Paragraph title: 2.4.1. In Vitro Synthesis of 35 S-Labeled DDB1 ... 35 S-labeled methionine: 1,000 Ci/mmol, 50 mCi/mL (GE Healthcare Life Sciences, Piscataway, NJ).

Immunoprecipitation:

Article Title: Deletion of the PAT1 Gene Affects Translation Initiation and Suppresses a PAB1 Gene Deletion in Yeast
Article Snippet: To measure the amounts of poly(A)- and cap-containing RNA, 1 ml of yeast culture was labeled for 5 min with 5 μCi of [8-3 H]adenine at a concentration of 24 Ci/mmol (Amersham). .. Cap+ RNA was isolated by immunoprecipitation with anti-cap antibodies as described by Muhlrad et al. ( ).

Fractionation:

Article Title: Deletion of the PAT1 Gene Affects Translation Initiation and Suppresses a PAB1 Gene Deletion in Yeast
Article Snippet: To measure the amounts of poly(A)- and cap-containing RNA, 1 ml of yeast culture was labeled for 5 min with 5 μCi of [8-3 H]adenine at a concentration of 24 Ci/mmol (Amersham). .. Poly(A) tail length was analyzed by RNA 3′-end labeling with [32 P]pCp (Amersham) and T4 RNA ligase, RNase digestion, and poly(A) fractionation on a denaturing polyacrylamide gel as previously described ( ).

Proliferation Assay:

Article Title: Interaction between complement receptor gC1qR and hepatitis C virus core protein inhibits T-lymphocyte proliferation
Article Snippet: Paragraph title: T-cell proliferation assay. ... At the 60-hour time point, 1 μCi/well of 3 (H)-thymidine was added (specific activity of 6.7 Ci/mmol; Amersham International, Oakville, Ontario, Canada).

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    Ci Mmol 2 Deoxy D, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ci mmol 2 deoxy d/product/GE Healthcare
    Average 84 stars, based on 1 article reviews
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    ci mmol 2 deoxy d - by Bioz Stars, 2020-04
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    Activity 67 Ci Mmol, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    activity 67 ci mmol - by Bioz Stars, 2020-04
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    GE Healthcare ci mmol
    Ci Mmol, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ci mmol/product/GE Healthcare
    Average 94 stars, based on 57 article reviews
    Price from $9.99 to $1999.99
    ci mmol - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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