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Agilent technologies chromatographic conditions uhplc
The identification of the active binding fraction of RP by CMC. ( a ) The experimental flow of CMC. PC-12 cells were incubated with RP (TEE) for 1 to 6 h. After incubation, chemical components without binding affinity to the cell membrane were washed away by PBS, while those components that bind on cell membrane were retained for analysis. The cells were then disrupted by citric acid buffer with ultrasound sonication. The lysate solution was then centrifuged, dried and re-dissolved in methanol. Cell lysate without RP incubation was collected as control. Finally, all the collected samples were analyzed using <t>UHPLC-TOF-MS.</t> ( b ) The Total Ion Chromatogram (TIC) of the 5 different batches of RP (TEE). ( c ) The TIC of the CMC samples. S1: The final PBS wash solution; S2: PC-12 lysate solution without RP (TEE) treatments; S3: RP (TEE) solution diluted with PBS; S4: PC-12 cell lysate solution collected after RP (TEE) treatments. The cluster of peaks (C5) indicated the chemical components that bind on the cell membrane of PC-12 cells. ( d ) The TIC of the CMC samples collected from 5 different batches of RP (TEE) treatments. A: PC-12 lysate solution without RP (TEE) treatments; B: The final PBS wash solution; C, E, G, I, K: 5 different batches of RP (TEE) solution diluted with PBS; D, F, H, J, L: PC-12 cell lysate solution collected after treatments of 5 different batches of RP (TEE). ( e ) The TIC of PC-12 cell lysate collected after 1 to 6 h of RP (TEE) treatments (500 μg/mL). ( f ) The TIC of PC-12 cell lysate collected after RP (TEE) treatments (125, 250, 500 or 750 μg/mL) for 4 h. All the samples were analyzed by UHPLC-TOF-MS on an <t>Agilent</t> Zorbax Eclipse Plus C-18 50 mm × 2.1 mm column (particle size: 1.8 μm) at a flow rate of 0.35 mL/min. The data was acquired in the scan mode from m/z 100 to 3200 Da with 2.0 spectra/s.
Chromatographic Conditions Uhplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 88 stars, based on 2 article reviews
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chromatographic conditions uhplc - by Bioz Stars, 2020-08
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1) Product Images from "Identification of novel autophagic Radix Polygalae fraction by cell membrane chromatography and UHPLC-(Q)TOF-MS for degradation of neurodegenerative disease proteins"

Article Title: Identification of novel autophagic Radix Polygalae fraction by cell membrane chromatography and UHPLC-(Q)TOF-MS for degradation of neurodegenerative disease proteins

Journal: Scientific Reports

doi: 10.1038/srep17199

The identification of the active binding fraction of RP by CMC. ( a ) The experimental flow of CMC. PC-12 cells were incubated with RP (TEE) for 1 to 6 h. After incubation, chemical components without binding affinity to the cell membrane were washed away by PBS, while those components that bind on cell membrane were retained for analysis. The cells were then disrupted by citric acid buffer with ultrasound sonication. The lysate solution was then centrifuged, dried and re-dissolved in methanol. Cell lysate without RP incubation was collected as control. Finally, all the collected samples were analyzed using UHPLC-TOF-MS. ( b ) The Total Ion Chromatogram (TIC) of the 5 different batches of RP (TEE). ( c ) The TIC of the CMC samples. S1: The final PBS wash solution; S2: PC-12 lysate solution without RP (TEE) treatments; S3: RP (TEE) solution diluted with PBS; S4: PC-12 cell lysate solution collected after RP (TEE) treatments. The cluster of peaks (C5) indicated the chemical components that bind on the cell membrane of PC-12 cells. ( d ) The TIC of the CMC samples collected from 5 different batches of RP (TEE) treatments. A: PC-12 lysate solution without RP (TEE) treatments; B: The final PBS wash solution; C, E, G, I, K: 5 different batches of RP (TEE) solution diluted with PBS; D, F, H, J, L: PC-12 cell lysate solution collected after treatments of 5 different batches of RP (TEE). ( e ) The TIC of PC-12 cell lysate collected after 1 to 6 h of RP (TEE) treatments (500 μg/mL). ( f ) The TIC of PC-12 cell lysate collected after RP (TEE) treatments (125, 250, 500 or 750 μg/mL) for 4 h. All the samples were analyzed by UHPLC-TOF-MS on an Agilent Zorbax Eclipse Plus C-18 50 mm × 2.1 mm column (particle size: 1.8 μm) at a flow rate of 0.35 mL/min. The data was acquired in the scan mode from m/z 100 to 3200 Da with 2.0 spectra/s.
Figure Legend Snippet: The identification of the active binding fraction of RP by CMC. ( a ) The experimental flow of CMC. PC-12 cells were incubated with RP (TEE) for 1 to 6 h. After incubation, chemical components without binding affinity to the cell membrane were washed away by PBS, while those components that bind on cell membrane were retained for analysis. The cells were then disrupted by citric acid buffer with ultrasound sonication. The lysate solution was then centrifuged, dried and re-dissolved in methanol. Cell lysate without RP incubation was collected as control. Finally, all the collected samples were analyzed using UHPLC-TOF-MS. ( b ) The Total Ion Chromatogram (TIC) of the 5 different batches of RP (TEE). ( c ) The TIC of the CMC samples. S1: The final PBS wash solution; S2: PC-12 lysate solution without RP (TEE) treatments; S3: RP (TEE) solution diluted with PBS; S4: PC-12 cell lysate solution collected after RP (TEE) treatments. The cluster of peaks (C5) indicated the chemical components that bind on the cell membrane of PC-12 cells. ( d ) The TIC of the CMC samples collected from 5 different batches of RP (TEE) treatments. A: PC-12 lysate solution without RP (TEE) treatments; B: The final PBS wash solution; C, E, G, I, K: 5 different batches of RP (TEE) solution diluted with PBS; D, F, H, J, L: PC-12 cell lysate solution collected after treatments of 5 different batches of RP (TEE). ( e ) The TIC of PC-12 cell lysate collected after 1 to 6 h of RP (TEE) treatments (500 μg/mL). ( f ) The TIC of PC-12 cell lysate collected after RP (TEE) treatments (125, 250, 500 or 750 μg/mL) for 4 h. All the samples were analyzed by UHPLC-TOF-MS on an Agilent Zorbax Eclipse Plus C-18 50 mm × 2.1 mm column (particle size: 1.8 μm) at a flow rate of 0.35 mL/min. The data was acquired in the scan mode from m/z 100 to 3200 Da with 2.0 spectra/s.

Techniques Used: Binding Assay, Flow Cytometry, Incubation, Sonication, Mass Spectrometry

2) Product Images from "The New Application of UHPLC-DAD-TOF/MS in Identification of Inhibitors on β-Amyloid Fibrillation From Scutellaria baicalensis"

Article Title: The New Application of UHPLC-DAD-TOF/MS in Identification of Inhibitors on β-Amyloid Fibrillation From Scutellaria baicalensis

Journal: Frontiers in Pharmacology

doi: 10.3389/fphar.2019.00194

Schematic diagram for the rationale, design and characterization of Aβ fibril inhibitors from herbal medicines. The incubation of Aβ (1–42) solution with TCMs extract. Analysis of the pre-incubated Aβ (1–42)-TCM extract mixture using Agilent 6230 UHPLC-DAD-TOF/MS instrument. Chemical analytical prediction on the binding propensity of the compounds by UHPLC-DAD-TOF/MS. Identification of the Aβ (1–42) binding chemical components from TCMs with their accurate mass identified and confirmed from literatures. Bioassay-validation of the Aβ (1–42) peptide-binding propensity of the identified compounds.
Figure Legend Snippet: Schematic diagram for the rationale, design and characterization of Aβ fibril inhibitors from herbal medicines. The incubation of Aβ (1–42) solution with TCMs extract. Analysis of the pre-incubated Aβ (1–42)-TCM extract mixture using Agilent 6230 UHPLC-DAD-TOF/MS instrument. Chemical analytical prediction on the binding propensity of the compounds by UHPLC-DAD-TOF/MS. Identification of the Aβ (1–42) binding chemical components from TCMs with their accurate mass identified and confirmed from literatures. Bioassay-validation of the Aβ (1–42) peptide-binding propensity of the identified compounds.

Techniques Used: Incubation, Mass Spectrometry, Binding Assay

The prediction of Aβ-binding propensity of chemical components in SB using Agilent 6230 UHPLC-DAD-TOF/MS . (A) The TIC of SB-TEE with or without the incubation with 20 and 200 μM Aβ (1–42). S1: SB-TEE; S2: The incubation of SB-TEE with 20 μM Aβ (1–42); S3: The incubation of SB-TEE with 200 μM Aβ (1–42). (B) The bar chart shows the percentage of reduction (%) in the peak area of each SB component in TIC after their incubation with 20 or 200 μM Aβ (1–42). Columns means of 3 independent experiments; bars, SEM. ∗ p
Figure Legend Snippet: The prediction of Aβ-binding propensity of chemical components in SB using Agilent 6230 UHPLC-DAD-TOF/MS . (A) The TIC of SB-TEE with or without the incubation with 20 and 200 μM Aβ (1–42). S1: SB-TEE; S2: The incubation of SB-TEE with 20 μM Aβ (1–42); S3: The incubation of SB-TEE with 200 μM Aβ (1–42). (B) The bar chart shows the percentage of reduction (%) in the peak area of each SB component in TIC after their incubation with 20 or 200 μM Aβ (1–42). Columns means of 3 independent experiments; bars, SEM. ∗ p

Techniques Used: Binding Assay, Mass Spectrometry, Incubation

The identification of the chemical components in SB using Agilent 6230 UHPLC-DAD-TOF/MS. (A) TIC (total ion chromatogram) and the corresponding UV chromatogram of SB-TEE and its main components (scutellarin, baicalin, oroxyloside, wogonoside, baicalein, wogonin and oroxylin A). (B) The chemical structures of the identified components in SB.
Figure Legend Snippet: The identification of the chemical components in SB using Agilent 6230 UHPLC-DAD-TOF/MS. (A) TIC (total ion chromatogram) and the corresponding UV chromatogram of SB-TEE and its main components (scutellarin, baicalin, oroxyloside, wogonoside, baicalein, wogonin and oroxylin A). (B) The chemical structures of the identified components in SB.

Techniques Used: Mass Spectrometry

Related Articles

Mass Spectrometry:

Article Title: The New Application of UHPLC-DAD-TOF/MS in Identification of Inhibitors on β-Amyloid Fibrillation From Scutellaria baicalensis
Article Snippet: .. Instrument and Chromatographic Conditions UHPLC (Agilent Technologies 1290 Series), equipped with the time of flight (TOF) MS (Agilent Technologies 6230) with a jet stream ion source, was operated in negative and positive ion modes during the UHPLC analysis. .. All samples were analyzed using the Agilent Eclipse Plus C-18 column (100 × 2.1 mm) with a particle size of 1.8 μm at a flow rate of 0.35 mL/min.

Article Title: Identification of novel autophagic Radix Polygalae fraction by cell membrane chromatography and UHPLC-(Q)TOF-MS for degradation of neurodegenerative disease proteins
Article Snippet: .. Instruments and chromatographic conditions UHPLC (Agilent Technologies 1290 Series) equipped with the time of flight MS (Agilent Technologies 6230) with a jet stream ion source was operated in negative ion mode during the UHPLC analysis. .. The samples were analyzed by using the Agilent Zorbax Eclipse Plus C-18 column with a particle size of 1.8 μm (flow rate: 0.35 mL/min).

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    Agilent technologies chromatographic conditionsan agilent uhplc system
    Chromatographic Conditionsan Agilent Uhplc System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chromatographic conditionsan agilent uhplc system/product/Agilent technologies
    Average 84 stars, based on 1 article reviews
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