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Merck KGaA chromatographic conditions chromatography
Chromatographic Conditions Chromatography, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chromatographic conditions chromatography/product/Merck KGaA
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
chromatographic conditions chromatography - by Bioz Stars, 2021-07
86/100 stars

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High Performance Liquid Chromatography:

Article Title: Ensuring Homogeneity in Powder Mixtures for Pharmaceuticals and Dietary Supplements: Evaluation of a 3-Axis Mixing Equipment
Article Snippet: HPLC mobile phases were filtered through a 0.45 μm filter membrane (RC-45/15 MS; Chromafil, Düren, Germany) and degassed using an ultrasonic apparatus (Model 1600A; Unique, Indaiatuba, Brazil) for 30 min immediately before use. .. HPLC analyses for finasteride, folic acid, and vitamin B12 were performed on a qualified and calibrated chromatography system (Young Lin, Anyang, Korea) composed of a quaternary gradient pump (YL 9110), a photodiode array (PDA) detector (YL 9160), a 96-vial programmable autosampler (YL 9150), a column oven compartment (YL 9130), a variable sample loop up to 200 mL, and a software controller (Clarity). .. Chromatographic conditions for finasteride quantification were as follows: column—C18 4.6 × 100 mm, at 45 °C; mobile phase—phosphoric acid 2.5mM and acetonitrile (1:1); flux—1.5 mL/min; volume of injection—20 µL; and UV detection at 240 nm.

Article Title: Assessment of xenoestrogenic exposure by a biomarker approach: application of the E-Screen bioassay to determine estrogenic response of serum extracts
Article Snippet: An aliquot of 100 μL was injected into the HPLC system. .. Apparatus and chromatographic conditions (HPLC and GC) High performance liquid chromatography separation of the extracted compounds was performed using a LaChrom HPLC system consisting of a L-7100 HPLC pump, a L-7200 Auto Sampler, a L-7400 UV-detector, a L-7450 Diode-array Detector, a L-7350 Column Thermostat and a D-7000 Interface Module (Merck, Darmstadt, Germany). .. Defined fractions of the mobile phase were collected by an L-7650 Fraction Collector (Merck, Darmstadt, Germany).

Article Title: Processing ‘Ataulfo’ Mango into Juice Preserves the Bioavailability and Antioxidant Capacity of Its Phenolic Compounds
Article Snippet: Briefly, 20 µL of vitamin C-EDTA (100 mg ascorbic acid plus 1 mg EDTA in 1.0 mL of 0.4 M NaH2 PO4 , pH 3.6), 20 µL of 10 µg/mL internal standard (2′,3′,4′-trihydroxyacetophenone), and 20 µL β-glucuronidase/sulfatase (98,000 kU/L β-glucuronidase and 2400 kU/L sulfatase) were added to 500 µL of plasma, and the mixture was incubated at 37 °C for 60 min. .. After centrifugation, the supernatant was removed, dried with nitrogen gas, and reconstituted in the aqueous HPLC mobile phase, filtered using Ultrafree Durapore Centrifugal filter devices (0.22, Merck Millipore, Billerica, MA, USA), and injected into the HPLC- ECD system, using previously optimized chromatographic conditions [ ]. ..

Chromatography:

Article Title: Ensuring Homogeneity in Powder Mixtures for Pharmaceuticals and Dietary Supplements: Evaluation of a 3-Axis Mixing Equipment
Article Snippet: HPLC mobile phases were filtered through a 0.45 μm filter membrane (RC-45/15 MS; Chromafil, Düren, Germany) and degassed using an ultrasonic apparatus (Model 1600A; Unique, Indaiatuba, Brazil) for 30 min immediately before use. .. HPLC analyses for finasteride, folic acid, and vitamin B12 were performed on a qualified and calibrated chromatography system (Young Lin, Anyang, Korea) composed of a quaternary gradient pump (YL 9110), a photodiode array (PDA) detector (YL 9160), a 96-vial programmable autosampler (YL 9150), a column oven compartment (YL 9130), a variable sample loop up to 200 mL, and a software controller (Clarity). .. Chromatographic conditions for finasteride quantification were as follows: column—C18 4.6 × 100 mm, at 45 °C; mobile phase—phosphoric acid 2.5mM and acetonitrile (1:1); flux—1.5 mL/min; volume of injection—20 µL; and UV detection at 240 nm.

Article Title: Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis
Article Snippet: Protein visualization was performed with Luminata Forte Western HRP substrate (Millipore, Billerica, MA, USA) on X-ray film (CL-Xposure™ film, Thermo Scientific, Rockford, IL, USA). .. Two-step purification method: cationic exchange chromatography and reverse phase-high performance liquid chromatography (RP-HPLC) A tangential filtration of the conditioned media was previously employed via a Labscale™ TFF System (Merck Millipore, Burlington, MA, USA) with a 5 kDa of molecular weight cutoff to remove any contaminants or reagents that may interfere in the purification. .. The first purification step consisted of a concentration and purification using an ion exchange chromatographic resin, cationic type, SP-Sepharose Fast Flow (GE Healthcare, Uppsala, Sweden) (Arthuso et al. ; Heller et al. ; Soares et al. ).

Software:

Article Title: Ensuring Homogeneity in Powder Mixtures for Pharmaceuticals and Dietary Supplements: Evaluation of a 3-Axis Mixing Equipment
Article Snippet: HPLC mobile phases were filtered through a 0.45 μm filter membrane (RC-45/15 MS; Chromafil, Düren, Germany) and degassed using an ultrasonic apparatus (Model 1600A; Unique, Indaiatuba, Brazil) for 30 min immediately before use. .. HPLC analyses for finasteride, folic acid, and vitamin B12 were performed on a qualified and calibrated chromatography system (Young Lin, Anyang, Korea) composed of a quaternary gradient pump (YL 9110), a photodiode array (PDA) detector (YL 9160), a 96-vial programmable autosampler (YL 9150), a column oven compartment (YL 9130), a variable sample loop up to 200 mL, and a software controller (Clarity). .. Chromatographic conditions for finasteride quantification were as follows: column—C18 4.6 × 100 mm, at 45 °C; mobile phase—phosphoric acid 2.5mM and acetonitrile (1:1); flux—1.5 mL/min; volume of injection—20 µL; and UV detection at 240 nm.

Thin Layer Chromatography:

Article Title: Development and Validation of a HPTLC Method for Simultaneous Quantitation of Flunarizine Dihydrochloride and Propranolol Hydrochloride in Capsule Dosage Form
Article Snippet: A Camag TLC system (Muttens, Switzerland) comprising of Camang automatic TLC sampler 4 (ATS4) applicator, syringe capacity 25 μl, Camang TLC scanner 4, Camag WinCATS software version 1.4.6.2002, Camag twin trough chambers (10×10 cm2 and 20×10 cm2 ), Camag TLC visualizer, ultrasonicator and centrifuge was used during the study. .. TLC plates used were precoated silica gel aluminium plate 60 F254 , 20×10 cm2 with 0.2 mm thickness (E. Merck, Mumbai, India). .. The chromatographic conditions maintained were precoated silica gel on aluminum plate 60 F254 , (20×10 cm2 and 10×10 cm2 , prewashed by methanol and activated at 60° for 5 min prior to chromatography) as stationary phase, ethyl acetate:methanol:glacial acetic acid in the proportion of 8:1:1, (v/v/v) as mobile phase for the FLU and PRO.

Article Title: Validation of a Thin-Layer Chromatography for the Determination of Hydrocortisone Acetate and Lidocaine in a Pharmaceutical Preparation
Article Snippet: 5 μ L of sample solution for the TLC-densitometric analysis and quantitative determination of examined substances, hydrocortisone acetate and lidocaine hydrochloride, was used in each case. .. Apparatus and Chromatographic Conditions A thin-layer chromatography in normal phase system (NP-TLC) was performed on 10 cm × 20 cm aluminum plates precoated with silica gel 60F254 from Merck KGaA (number 1.05570, Darmstadt, Germany). .. Next, 5 μ L of sample was spotted by means of a Camag (Muttenz, Switzerland) micropipette (5 μ L).

Purification:

Article Title: Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis
Article Snippet: Protein visualization was performed with Luminata Forte Western HRP substrate (Millipore, Billerica, MA, USA) on X-ray film (CL-Xposure™ film, Thermo Scientific, Rockford, IL, USA). .. Two-step purification method: cationic exchange chromatography and reverse phase-high performance liquid chromatography (RP-HPLC) A tangential filtration of the conditioned media was previously employed via a Labscale™ TFF System (Merck Millipore, Burlington, MA, USA) with a 5 kDa of molecular weight cutoff to remove any contaminants or reagents that may interfere in the purification. .. The first purification step consisted of a concentration and purification using an ion exchange chromatographic resin, cationic type, SP-Sepharose Fast Flow (GE Healthcare, Uppsala, Sweden) (Arthuso et al. ; Heller et al. ; Soares et al. ).

Liquid Chromatography:

Article Title: Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis
Article Snippet: Protein visualization was performed with Luminata Forte Western HRP substrate (Millipore, Billerica, MA, USA) on X-ray film (CL-Xposure™ film, Thermo Scientific, Rockford, IL, USA). .. Two-step purification method: cationic exchange chromatography and reverse phase-high performance liquid chromatography (RP-HPLC) A tangential filtration of the conditioned media was previously employed via a Labscale™ TFF System (Merck Millipore, Burlington, MA, USA) with a 5 kDa of molecular weight cutoff to remove any contaminants or reagents that may interfere in the purification. .. The first purification step consisted of a concentration and purification using an ion exchange chromatographic resin, cationic type, SP-Sepharose Fast Flow (GE Healthcare, Uppsala, Sweden) (Arthuso et al. ; Heller et al. ; Soares et al. ).

Filtration:

Article Title: Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis
Article Snippet: Protein visualization was performed with Luminata Forte Western HRP substrate (Millipore, Billerica, MA, USA) on X-ray film (CL-Xposure™ film, Thermo Scientific, Rockford, IL, USA). .. Two-step purification method: cationic exchange chromatography and reverse phase-high performance liquid chromatography (RP-HPLC) A tangential filtration of the conditioned media was previously employed via a Labscale™ TFF System (Merck Millipore, Burlington, MA, USA) with a 5 kDa of molecular weight cutoff to remove any contaminants or reagents that may interfere in the purification. .. The first purification step consisted of a concentration and purification using an ion exchange chromatographic resin, cationic type, SP-Sepharose Fast Flow (GE Healthcare, Uppsala, Sweden) (Arthuso et al. ; Heller et al. ; Soares et al. ).

Molecular Weight:

Article Title: Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis
Article Snippet: Protein visualization was performed with Luminata Forte Western HRP substrate (Millipore, Billerica, MA, USA) on X-ray film (CL-Xposure™ film, Thermo Scientific, Rockford, IL, USA). .. Two-step purification method: cationic exchange chromatography and reverse phase-high performance liquid chromatography (RP-HPLC) A tangential filtration of the conditioned media was previously employed via a Labscale™ TFF System (Merck Millipore, Burlington, MA, USA) with a 5 kDa of molecular weight cutoff to remove any contaminants or reagents that may interfere in the purification. .. The first purification step consisted of a concentration and purification using an ion exchange chromatographic resin, cationic type, SP-Sepharose Fast Flow (GE Healthcare, Uppsala, Sweden) (Arthuso et al. ; Heller et al. ; Soares et al. ).

Centrifugation:

Article Title: Processing ‘Ataulfo’ Mango into Juice Preserves the Bioavailability and Antioxidant Capacity of Its Phenolic Compounds
Article Snippet: Briefly, 20 µL of vitamin C-EDTA (100 mg ascorbic acid plus 1 mg EDTA in 1.0 mL of 0.4 M NaH2 PO4 , pH 3.6), 20 µL of 10 µg/mL internal standard (2′,3′,4′-trihydroxyacetophenone), and 20 µL β-glucuronidase/sulfatase (98,000 kU/L β-glucuronidase and 2400 kU/L sulfatase) were added to 500 µL of plasma, and the mixture was incubated at 37 °C for 60 min. .. After centrifugation, the supernatant was removed, dried with nitrogen gas, and reconstituted in the aqueous HPLC mobile phase, filtered using Ultrafree Durapore Centrifugal filter devices (0.22, Merck Millipore, Billerica, MA, USA), and injected into the HPLC- ECD system, using previously optimized chromatographic conditions [ ]. ..

Injection:

Article Title: Processing ‘Ataulfo’ Mango into Juice Preserves the Bioavailability and Antioxidant Capacity of Its Phenolic Compounds
Article Snippet: Briefly, 20 µL of vitamin C-EDTA (100 mg ascorbic acid plus 1 mg EDTA in 1.0 mL of 0.4 M NaH2 PO4 , pH 3.6), 20 µL of 10 µg/mL internal standard (2′,3′,4′-trihydroxyacetophenone), and 20 µL β-glucuronidase/sulfatase (98,000 kU/L β-glucuronidase and 2400 kU/L sulfatase) were added to 500 µL of plasma, and the mixture was incubated at 37 °C for 60 min. .. After centrifugation, the supernatant was removed, dried with nitrogen gas, and reconstituted in the aqueous HPLC mobile phase, filtered using Ultrafree Durapore Centrifugal filter devices (0.22, Merck Millipore, Billerica, MA, USA), and injected into the HPLC- ECD system, using previously optimized chromatographic conditions [ ]. ..

High Performance Thin Layer Chromatography:

Article Title: Pharmacognostical and high performance thin layer chromatography studies on leaves of Clerodendrum infortunatum L.
Article Snippet: .. Instrumentation and chromatographic conditions High-performance thin layer chromatography was performed on 10 cm × 10 cm aluminum backed plates coated with Silica gel GF254 (Merck, Mumbai, India). .. Standard solution of gallic acid and sample solution and tyrosine and sample solution was applied to the separate plates maintaining a distance of 10 mm from the edge of plate and 10.0 mm from the bottom edge of the same chromatographic plate by use of a Camag (Muttenz, Switzerland) Linomat V sample applicator equipped with a 2 μL Hamilton (USA) syringe.

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    Merck KGaA chromatographic conditions chromatography
    Chromatographic Conditions Chromatography, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chromatographic conditions chromatography/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chromatographic conditions chromatography - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    86
    Merck KGaA aluminum plates silica gel 60f254
    Aluminum Plates Silica Gel 60f254, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aluminum plates silica gel 60f254/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aluminum plates silica gel 60f254 - by Bioz Stars, 2021-07
    86/100 stars
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    86
    Merck KGaA thin layer chromatography
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    https://www.bioz.com/result/thin layer chromatography/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thin layer chromatography - by Bioz Stars, 2021-07
    86/100 stars
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    86
    Merck KGaA chromatographic conditions high performance liquid chromatography grade acetonitrile
    Chromatographic Conditions High Performance Liquid Chromatography Grade Acetonitrile, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chromatographic conditions high performance liquid chromatography grade acetonitrile/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chromatographic conditions high performance liquid chromatography grade acetonitrile - by Bioz Stars, 2021-07
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