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Merck & Co chromatographic conditions chromatography
Chromatographic Conditions Chromatography, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chromatographic conditions chromatography/product/Merck & Co
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
chromatographic conditions chromatography - by Bioz Stars, 2021-07
86/100 stars

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Related Articles

Expressing:

Article Title: Increased plasma phenylacetic acid in patients with end-stage renal failure inhibits iNOS expression
Article Snippet: After each chromatographic step, the influence of the fractions on the iNOS expression was assayed. .. Next, the fractions decreasing the iNOS expression were fractionated (flow: 0.5 ml/min) on a C18 reversed-phase HPLC column (LiChrosorb RP C18, 250 × 4.6 mm; Merck) with 0.1% trifluoroacetic acid (TFA) in H2 O as eluent A and 0.1% TFA in CH3 CN as eluent B under the following gradient condition: 0–5 minutes, 0–20% eluent B; 5 to 10 minutes, 20% eluent B; 10–30 minutes, 20–40% eluent B; 30–40 minutes, 40–60% eluent B; 40–45 minutes, 60–100% eluent B; 45–50 minutes, 100% eluent B. .. Subsequently, the fractions decreasing the iNOS expression were chromatographed (flow: 0.5 ml/min) on an additional reversed-phase column (Supersphere RP C18 endcapped, 250 × 4.6 mm; Merck) with the following gradient: 0–10 minutes, 100% eluent A (20 mmol/l TEAA in water); 10–20 minutes, 0–20% eluent B (20 mmol/l TEAA in CH3 CN); 20–40 minutes, 20–40% eluent B; 40–50 minutes, 40–60% eluent B; 50–55 minutes, 60–100% eluent B; 55–60 minutes, 100% eluent B).

Flow Cytometry:

Article Title: Increased plasma phenylacetic acid in patients with end-stage renal failure inhibits iNOS expression
Article Snippet: After each chromatographic step, the influence of the fractions on the iNOS expression was assayed. .. Next, the fractions decreasing the iNOS expression were fractionated (flow: 0.5 ml/min) on a C18 reversed-phase HPLC column (LiChrosorb RP C18, 250 × 4.6 mm; Merck) with 0.1% trifluoroacetic acid (TFA) in H2 O as eluent A and 0.1% TFA in CH3 CN as eluent B under the following gradient condition: 0–5 minutes, 0–20% eluent B; 5 to 10 minutes, 20% eluent B; 10–30 minutes, 20–40% eluent B; 30–40 minutes, 40–60% eluent B; 40–45 minutes, 60–100% eluent B; 45–50 minutes, 100% eluent B. .. Subsequently, the fractions decreasing the iNOS expression were chromatographed (flow: 0.5 ml/min) on an additional reversed-phase column (Supersphere RP C18 endcapped, 250 × 4.6 mm; Merck) with the following gradient: 0–10 minutes, 100% eluent A (20 mmol/l TEAA in water); 10–20 minutes, 0–20% eluent B (20 mmol/l TEAA in CH3 CN); 20–40 minutes, 20–40% eluent B; 40–50 minutes, 40–60% eluent B; 50–55 minutes, 60–100% eluent B; 55–60 minutes, 100% eluent B).

Article Title: Activity of daptomycin- and vancomycin-loaded poly-epsilon-caprolactone microparticles against mature staphylococcal biofilms
Article Snippet: Released antibiotics were quantified by previously optimized high-performance liquid chromatography methods (Beckman Coulter System Gold with 126 solvent module and 166 UV-Vis detector coupled with a Stark Holland Midas autosampler). .. Briefly, quantification of daptomycin was performed using a Merck LiChospher 125-4, RP18 5 µm, LiChroCART 100 column and the following chromatographic conditions: 0.7 mL/min flow; injection volume of 20 µL; mobile phase of 35% acetonitrile and 65% PBS (pH 7.4), and detection at 230 nm. .. As for vancomycin, the same type of column was used and the chromatographic conditions were as follows: 1.2 mL/min flow; injection volume of 40 µL; mobile phase of 10% acetonitrile and 90% H2 KPO4 (pH 2.75), and detection at 280 nm.

Article Title: Kinetics of Antigen Expression and Epitope Presentation during Virus Infection
Article Snippet: .. 1 nmol of each peptide was spiked individually onto a 4.6 mm internal diameter×50 mm long reversed-phase C18 HPLC column (Chromolith Speed Rod, Merck) using an ÄKTAmicro HPLC system (GE Healthcare) running on a mobile phase buffer A of 0.1% trifluoroacetic acid (TFA) and buffer B of 80% acetonitrile/0.1% TFA and at a flow rate of 1 ml/min. ..

High Performance Liquid Chromatography:

Article Title: Increased plasma phenylacetic acid in patients with end-stage renal failure inhibits iNOS expression
Article Snippet: After each chromatographic step, the influence of the fractions on the iNOS expression was assayed. .. Next, the fractions decreasing the iNOS expression were fractionated (flow: 0.5 ml/min) on a C18 reversed-phase HPLC column (LiChrosorb RP C18, 250 × 4.6 mm; Merck) with 0.1% trifluoroacetic acid (TFA) in H2 O as eluent A and 0.1% TFA in CH3 CN as eluent B under the following gradient condition: 0–5 minutes, 0–20% eluent B; 5 to 10 minutes, 20% eluent B; 10–30 minutes, 20–40% eluent B; 30–40 minutes, 40–60% eluent B; 40–45 minutes, 60–100% eluent B; 45–50 minutes, 100% eluent B. .. Subsequently, the fractions decreasing the iNOS expression were chromatographed (flow: 0.5 ml/min) on an additional reversed-phase column (Supersphere RP C18 endcapped, 250 × 4.6 mm; Merck) with the following gradient: 0–10 minutes, 100% eluent A (20 mmol/l TEAA in water); 10–20 minutes, 0–20% eluent B (20 mmol/l TEAA in CH3 CN); 20–40 minutes, 20–40% eluent B; 40–50 minutes, 40–60% eluent B; 50–55 minutes, 60–100% eluent B; 55–60 minutes, 100% eluent B).

Article Title: HPLC-DAD Determination of Nitrite and Nitrate in Human Saliva Utilizing a Phosphatidylcholine Column
Article Snippet: .. HPLC Conditions Chromatographic measurements were made on a LaChrom HPLC Merck Hitachi (E. Merck, Darmstadt, Germany) equipped with diode array detector (L-7455), pump (L-7100), interface (D-7000) and solvent degasser (L-7612). .. The chromatographic column IAM.PC.DD2 Regis HPLC (4.6 × 150 mm, 10 µm, pore size: 300 Å) was purchased from Agilent Technologies (Santa Clara, CA, USA).

Article Title: β-Sitosterol Loaded Nanostructured Lipid Carrier: Physical and Oxidative Stability, In Vitro Simulated Digestion and Hypocholesterolemic Activity
Article Snippet: .. Determination of β-Sitosterol ContentThe β-sitosterol was extracted by saponification with ethanolic KOH and analyzed using the reverse-phase HPLC (Merck Hitachi, Darmstadt, Germany) method. ..

Article Title: EROD and MROD as Markers of Cytochrome P450 1A Activities in Hepatic Microsomes from Entire and Castrated Male Pigs
Article Snippet: Instrumentation and chromatographic conditions Resorufin quantification by HPLC was based on a previously described method [ ]. .. Chromatography was carried out with a pumping system (L-6200A), autosampler (AS 2000), fluorescence detector (L-7480) and D-6000 HPLC Manager software (Merck, Hitachi, Tokyo, Japan). .. The samples (5 μL) were injected onto a Hypersil ODS column (3 μm, 60 × 4.6 mm, Hewlett–Packard) equipped with a guard column.

Article Title: Kinetics of Antigen Expression and Epitope Presentation during Virus Infection
Article Snippet: .. 1 nmol of each peptide was spiked individually onto a 4.6 mm internal diameter×50 mm long reversed-phase C18 HPLC column (Chromolith Speed Rod, Merck) using an ÄKTAmicro HPLC system (GE Healthcare) running on a mobile phase buffer A of 0.1% trifluoroacetic acid (TFA) and buffer B of 80% acetonitrile/0.1% TFA and at a flow rate of 1 ml/min. ..

Injection:

Article Title: Activity of daptomycin- and vancomycin-loaded poly-epsilon-caprolactone microparticles against mature staphylococcal biofilms
Article Snippet: Released antibiotics were quantified by previously optimized high-performance liquid chromatography methods (Beckman Coulter System Gold with 126 solvent module and 166 UV-Vis detector coupled with a Stark Holland Midas autosampler). .. Briefly, quantification of daptomycin was performed using a Merck LiChospher 125-4, RP18 5 µm, LiChroCART 100 column and the following chromatographic conditions: 0.7 mL/min flow; injection volume of 20 µL; mobile phase of 35% acetonitrile and 65% PBS (pH 7.4), and detection at 230 nm. .. As for vancomycin, the same type of column was used and the chromatographic conditions were as follows: 1.2 mL/min flow; injection volume of 40 µL; mobile phase of 10% acetonitrile and 90% H2 KPO4 (pH 2.75), and detection at 280 nm.

Chromatography:

Article Title: EROD and MROD as Markers of Cytochrome P450 1A Activities in Hepatic Microsomes from Entire and Castrated Male Pigs
Article Snippet: Instrumentation and chromatographic conditions Resorufin quantification by HPLC was based on a previously described method [ ]. .. Chromatography was carried out with a pumping system (L-6200A), autosampler (AS 2000), fluorescence detector (L-7480) and D-6000 HPLC Manager software (Merck, Hitachi, Tokyo, Japan). .. The samples (5 μL) were injected onto a Hypersil ODS column (3 μm, 60 × 4.6 mm, Hewlett–Packard) equipped with a guard column.

Fluorescence:

Article Title: EROD and MROD as Markers of Cytochrome P450 1A Activities in Hepatic Microsomes from Entire and Castrated Male Pigs
Article Snippet: Instrumentation and chromatographic conditions Resorufin quantification by HPLC was based on a previously described method [ ]. .. Chromatography was carried out with a pumping system (L-6200A), autosampler (AS 2000), fluorescence detector (L-7480) and D-6000 HPLC Manager software (Merck, Hitachi, Tokyo, Japan). .. The samples (5 μL) were injected onto a Hypersil ODS column (3 μm, 60 × 4.6 mm, Hewlett–Packard) equipped with a guard column.

Software:

Article Title: EROD and MROD as Markers of Cytochrome P450 1A Activities in Hepatic Microsomes from Entire and Castrated Male Pigs
Article Snippet: Instrumentation and chromatographic conditions Resorufin quantification by HPLC was based on a previously described method [ ]. .. Chromatography was carried out with a pumping system (L-6200A), autosampler (AS 2000), fluorescence detector (L-7480) and D-6000 HPLC Manager software (Merck, Hitachi, Tokyo, Japan). .. The samples (5 μL) were injected onto a Hypersil ODS column (3 μm, 60 × 4.6 mm, Hewlett–Packard) equipped with a guard column.

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