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Effects of gomisin E on the cholinergic pathway in D-gal/PCPA–induced aging-related insomnia rats. (A – F) ACh content and activities of ChAT and AChE in the hippocampus and ileum determined by commercial assay kits. (G) Protein expression levels of <t>CHRM1</t> and CHRM2 in the hippocampus detected by WB and corresponding quantitative analysis. ** p < 0.01,**** p < 0.0001 vs. NC group. # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 vs. model group.
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Effects of gomisin E on the cholinergic pathway in D-gal/PCPA–induced aging-related insomnia rats. (A – F) ACh content and activities of ChAT and AChE in the hippocampus and ileum determined by commercial assay kits. (G) Protein expression levels of <t>CHRM1</t> and CHRM2 in the hippocampus detected by WB and corresponding quantitative analysis. ** p < 0.01,**** p < 0.0001 vs. NC group. # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 vs. model group.
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Effects of gomisin E on the cholinergic pathway in D-gal/PCPA–induced aging-related insomnia rats. (A – F) ACh content and activities of ChAT and AChE in the hippocampus and ileum determined by commercial assay kits. (G) Protein expression levels of <t>CHRM1</t> and CHRM2 in the hippocampus detected by WB and corresponding quantitative analysis. ** p < 0.01,**** p < 0.0001 vs. NC group. # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 vs. model group.
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Effects of gomisin E on the cholinergic pathway in D-gal/PCPA–induced aging-related insomnia rats. (A – F) ACh content and activities of ChAT and AChE in the hippocampus and ileum determined by commercial assay kits. (G) Protein expression levels of <t>CHRM1</t> and CHRM2 in the hippocampus detected by WB and corresponding quantitative analysis. ** p < 0.01,**** p < 0.0001 vs. NC group. # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 vs. model group.
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Effects of gomisin E on the cholinergic pathway in D-gal/PCPA–induced aging-related insomnia rats. (A – F) ACh content and activities of ChAT and AChE in the hippocampus and ileum determined by commercial assay kits. (G) Protein expression levels of <t>CHRM1</t> and CHRM2 in the hippocampus detected by WB and corresponding quantitative analysis. ** p < 0.01,**** p < 0.0001 vs. NC group. # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 vs. model group.
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Effects of gomisin E on the cholinergic pathway in D-gal/PCPA–induced aging-related insomnia rats. (A – F) ACh content and activities of ChAT and AChE in the hippocampus and ileum determined by commercial assay kits. (G) Protein expression levels of <t>CHRM1</t> and CHRM2 in the hippocampus detected by WB and corresponding quantitative analysis. ** p < 0.01,**** p < 0.0001 vs. NC group. # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 vs. model group.
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Effects of gomisin E on the cholinergic pathway in D-gal/PCPA–induced aging-related insomnia rats. (A – F) ACh content and activities of ChAT and AChE in the hippocampus and ileum determined by commercial assay kits. (G) Protein expression levels of <t>CHRM1</t> and CHRM2 in the hippocampus detected by WB and corresponding quantitative analysis. ** p < 0.01,**** p < 0.0001 vs. NC group. # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 vs. model group.
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Effects of gomisin E on the cholinergic pathway in D-gal/PCPA–induced aging-related insomnia rats. (A – F) ACh content and activities of ChAT and AChE in the hippocampus and ileum determined by commercial assay kits. (G) Protein expression levels of CHRM1 and CHRM2 in the hippocampus detected by WB and corresponding quantitative analysis. ** p < 0.01,**** p < 0.0001 vs. NC group. # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 vs. model group.

Journal: Frontiers in Psychology

Article Title: Gomisin E attenuates pentobarbital-induced sleep deficits in aging-related insomnia: an in vivo and in silico study implicating cholinergic signaling

doi: 10.3389/fpsyg.2026.1821889

Figure Lengend Snippet: Effects of gomisin E on the cholinergic pathway in D-gal/PCPA–induced aging-related insomnia rats. (A – F) ACh content and activities of ChAT and AChE in the hippocampus and ileum determined by commercial assay kits. (G) Protein expression levels of CHRM1 and CHRM2 in the hippocampus detected by WB and corresponding quantitative analysis. ** p < 0.01,**** p < 0.0001 vs. NC group. # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 vs. model group.

Article Snippet: Primary antibodies against CHRM1 (A1598), CHRM2 (DF4908) and GAPDH (A19056) were obtained from Abclonal, Affinity and Abclonal.

Techniques: Expressing