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Effect of the insertion of a neo marker knockout cassette upstream of Bag1 on head-to-head gene <t>Chmp5</t> expression. (a) Chromosomal region of Bag1 (exon 1–7)-Chmp5 (exon 1–8), including the predicted regulatory future from the Ensemble regulation resources ( https://oct2022.archive.ensembl.org/Mus_musculus/Gene/Summary?db=core;g=ENSMUSG00000028419;r = 4:40945802–40951599;t = ENSMUST00000030128;gene_summary=regulatory_build=normal ). Constructs of Chmp5-fused nLuc reporter genes carrying 8.4 kbp (F1-cLuc) and 5.0 kbp (F3-cLuc) are shown. The upstream region was replaced with the reporters, with the neo cassettes illustrated (F1-neo-nLuc and F3-neo-nLuc; see text for detail). (b) nLuc activity was observed by 3 different experiments (n = 3). Normalized activity of F1-nLuc (F1), F1-neo-nLuc (F1neo), F3-nLuc (F3), and F3-neo-nLuc (F3neo) is shown. The values are shown as the means ± SEM. Significance was determined using the Student’s t-test. Abbreviation used: SEM, standard error of the mean.
Chmp5, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bag1 chmp5 mouse genome regions  (TaKaRa)
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TaKaRa bag1 chmp5 mouse genome regions
Effect of the insertion of a neo marker knockout cassette upstream of Bag1 on head-to-head gene <t>Chmp5</t> expression. (a) Chromosomal region of Bag1 (exon 1–7)-Chmp5 (exon 1–8), including the predicted regulatory future from the Ensemble regulation resources ( https://oct2022.archive.ensembl.org/Mus_musculus/Gene/Summary?db=core;g=ENSMUSG00000028419;r = 4:40945802–40951599;t = ENSMUST00000030128;gene_summary=regulatory_build=normal ). Constructs of Chmp5-fused nLuc reporter genes carrying 8.4 kbp (F1-cLuc) and 5.0 kbp (F3-cLuc) are shown. The upstream region was replaced with the reporters, with the neo cassettes illustrated (F1-neo-nLuc and F3-neo-nLuc; see text for detail). (b) nLuc activity was observed by 3 different experiments (n = 3). Normalized activity of F1-nLuc (F1), F1-neo-nLuc (F1neo), F3-nLuc (F3), and F3-neo-nLuc (F3neo) is shown. The values are shown as the means ± SEM. Significance was determined using the Student’s t-test. Abbreviation used: SEM, standard error of the mean.
Bag1 Chmp5 Mouse Genome Regions, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chmp5 expression  (Promega)
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Promega chmp5 expression
Effect of the insertion of a neo marker knockout cassette upstream of Bag1 on head-to-head gene <t>Chmp5</t> expression. (a) Chromosomal region of Bag1 (exon 1–7)-Chmp5 (exon 1–8), including the predicted regulatory future from the Ensemble regulation resources ( https://oct2022.archive.ensembl.org/Mus_musculus/Gene/Summary?db=core;g=ENSMUSG00000028419;r = 4:40945802–40951599;t = ENSMUST00000030128;gene_summary=regulatory_build=normal ). Constructs of Chmp5-fused nLuc reporter genes carrying 8.4 kbp (F1-cLuc) and 5.0 kbp (F3-cLuc) are shown. The upstream region was replaced with the reporters, with the neo cassettes illustrated (F1-neo-nLuc and F3-neo-nLuc; see text for detail). (b) nLuc activity was observed by 3 different experiments (n = 3). Normalized activity of F1-nLuc (F1), F1-neo-nLuc (F1neo), F3-nLuc (F3), and F3-neo-nLuc (F3neo) is shown. The values are shown as the means ± SEM. Significance was determined using the Student’s t-test. Abbreviation used: SEM, standard error of the mean.
Chmp5 Expression, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chmp5 gene expression  (Promega)
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Promega chmp5 gene expression
Effect of the insertion of a neo marker knockout cassette upstream of Bag1 on head-to-head gene <t>Chmp5</t> expression. (a) Chromosomal region of Bag1 (exon 1–7)-Chmp5 (exon 1–8), including the predicted regulatory future from the Ensemble regulation resources ( https://oct2022.archive.ensembl.org/Mus_musculus/Gene/Summary?db=core;g=ENSMUSG00000028419;r = 4:40945802–40951599;t = ENSMUST00000030128;gene_summary=regulatory_build=normal ). Constructs of Chmp5-fused nLuc reporter genes carrying 8.4 kbp (F1-cLuc) and 5.0 kbp (F3-cLuc) are shown. The upstream region was replaced with the reporters, with the neo cassettes illustrated (F1-neo-nLuc and F3-neo-nLuc; see text for detail). (b) nLuc activity was observed by 3 different experiments (n = 3). Normalized activity of F1-nLuc (F1), F1-neo-nLuc (F1neo), F3-nLuc (F3), and F3-neo-nLuc (F3neo) is shown. The values are shown as the means ± SEM. Significance was determined using the Student’s t-test. Abbreviation used: SEM, standard error of the mean.
Chmp5 Gene Expression, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chmp5 transcription  (TaKaRa)
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TaKaRa chmp5 transcription
Effect of the insertion of a neo marker knockout cassette upstream of Bag1 on head-to-head gene <t>Chmp5</t> expression. (a) Chromosomal region of Bag1 (exon 1–7)-Chmp5 (exon 1–8), including the predicted regulatory future from the Ensemble regulation resources ( https://oct2022.archive.ensembl.org/Mus_musculus/Gene/Summary?db=core;g=ENSMUSG00000028419;r = 4:40945802–40951599;t = ENSMUST00000030128;gene_summary=regulatory_build=normal ). Constructs of Chmp5-fused nLuc reporter genes carrying 8.4 kbp (F1-cLuc) and 5.0 kbp (F3-cLuc) are shown. The upstream region was replaced with the reporters, with the neo cassettes illustrated (F1-neo-nLuc and F3-neo-nLuc; see text for detail). (b) nLuc activity was observed by 3 different experiments (n = 3). Normalized activity of F1-nLuc (F1), F1-neo-nLuc (F1neo), F3-nLuc (F3), and F3-neo-nLuc (F3neo) is shown. The values are shown as the means ± SEM. Significance was determined using the Student’s t-test. Abbreviation used: SEM, standard error of the mean.
Chmp5 Transcription, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibodies against chmp5  (Affinity Biosciences)
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Affinity Biosciences antibodies against chmp5
Effect of the insertion of a neo marker knockout cassette upstream of Bag1 on head-to-head gene <t>Chmp5</t> expression. (a) Chromosomal region of Bag1 (exon 1–7)-Chmp5 (exon 1–8), including the predicted regulatory future from the Ensemble regulation resources ( https://oct2022.archive.ensembl.org/Mus_musculus/Gene/Summary?db=core;g=ENSMUSG00000028419;r = 4:40945802–40951599;t = ENSMUST00000030128;gene_summary=regulatory_build=normal ). Constructs of Chmp5-fused nLuc reporter genes carrying 8.4 kbp (F1-cLuc) and 5.0 kbp (F3-cLuc) are shown. The upstream region was replaced with the reporters, with the neo cassettes illustrated (F1-neo-nLuc and F3-neo-nLuc; see text for detail). (b) nLuc activity was observed by 3 different experiments (n = 3). Normalized activity of F1-nLuc (F1), F1-neo-nLuc (F1neo), F3-nLuc (F3), and F3-neo-nLuc (F3neo) is shown. The values are shown as the means ± SEM. Significance was determined using the Student’s t-test. Abbreviation used: SEM, standard error of the mean.
Antibodies Against Chmp5, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effect of the insertion of a neo marker knockout cassette upstream of Bag1 on head-to-head gene Chmp5 expression. (a) Chromosomal region of Bag1 (exon 1–7)-Chmp5 (exon 1–8), including the predicted regulatory future from the Ensemble regulation resources ( https://oct2022.archive.ensembl.org/Mus_musculus/Gene/Summary?db=core;g=ENSMUSG00000028419;r = 4:40945802–40951599;t = ENSMUST00000030128;gene_summary=regulatory_build=normal ). Constructs of Chmp5-fused nLuc reporter genes carrying 8.4 kbp (F1-cLuc) and 5.0 kbp (F3-cLuc) are shown. The upstream region was replaced with the reporters, with the neo cassettes illustrated (F1-neo-nLuc and F3-neo-nLuc; see text for detail). (b) nLuc activity was observed by 3 different experiments (n = 3). Normalized activity of F1-nLuc (F1), F1-neo-nLuc (F1neo), F3-nLuc (F3), and F3-neo-nLuc (F3neo) is shown. The values are shown as the means ± SEM. Significance was determined using the Student’s t-test. Abbreviation used: SEM, standard error of the mean.

Journal: Cell Stress & Chaperones

Article Title: Bag1 protein loss sensitizes mouse embryonic fibroblasts to glutathione depletion

doi: 10.1016/j.cstres.2024.05.003

Figure Lengend Snippet: Effect of the insertion of a neo marker knockout cassette upstream of Bag1 on head-to-head gene Chmp5 expression. (a) Chromosomal region of Bag1 (exon 1–7)-Chmp5 (exon 1–8), including the predicted regulatory future from the Ensemble regulation resources ( https://oct2022.archive.ensembl.org/Mus_musculus/Gene/Summary?db=core;g=ENSMUSG00000028419;r = 4:40945802–40951599;t = ENSMUST00000030128;gene_summary=regulatory_build=normal ). Constructs of Chmp5-fused nLuc reporter genes carrying 8.4 kbp (F1-cLuc) and 5.0 kbp (F3-cLuc) are shown. The upstream region was replaced with the reporters, with the neo cassettes illustrated (F1-neo-nLuc and F3-neo-nLuc; see text for detail). (b) nLuc activity was observed by 3 different experiments (n = 3). Normalized activity of F1-nLuc (F1), F1-neo-nLuc (F1neo), F3-nLuc (F3), and F3-neo-nLuc (F3neo) is shown. The values are shown as the means ± SEM. Significance was determined using the Student’s t-test. Abbreviation used: SEM, standard error of the mean.

Article Snippet: Then, to examine whether gene replacement affects the expression of Chmp5 gene expression, we constructed nanoLuc luciferase reporter (nLuc; Promega) genes for Chmp5 expression, in which the second exon of Chmp5 was fused in-frame with the N-terminus of nLuc.

Techniques: Marker, Knock-Out, Expressing, Construct, Activity Assay