chikv antigen  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Name:
    Chikungunya virus immune ascitic fluid V 548 701 562
    Description:
    Source Mouse
    Catalog Number:
    VR-1241AF
    Price:
    None
    Source:
    Mouse
    Buy from Supplier


    Structured Review

    ATCC chikv antigen
    Source Mouse
    https://www.bioz.com/result/chikv antigen/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chikv antigen - by Bioz Stars, 2021-05
    94/100 stars

    Images

    Related Articles

    other:

    Article Title: Fragmentation Study, Dual Anti-Bactericidal and Anti-Viral Effects and Molecular Docking of Cobalt(III) Complexes
    Article Snippet: Thus, this study is expected to provide new structural information and biological insights of cobalt(III) thiosemicarbazone-based compounds, encouraging the investigation of this substance as an alternative treatment for dental bacteria and chikungunya virus.

    Mouse Assay:

    Article Title: A VLP vaccine for epidemic Chikungunya virus protects non-human primates against infection
    Article Snippet: .. Next, we examined a commercially available immune serum from mice infected with an established CHIKV strain (ATCC). .. Incubation of immune sera with the CHIKV pseudotyped lentiviral vector, but not a VSV-G pseudotyped vector, inhibited entry ( ).

    Infection:

    Article Title: A VLP vaccine for epidemic Chikungunya virus protects non-human primates against infection
    Article Snippet: .. Next, we examined a commercially available immune serum from mice infected with an established CHIKV strain (ATCC). .. Incubation of immune sera with the CHIKV pseudotyped lentiviral vector, but not a VSV-G pseudotyped vector, inhibited entry ( ).

    Staining:

    Article Title: DDX56 Binds to Chikungunya Virus RNA To Control Infection
    Article Snippet: At the indicated time points, cells were washed once with phosphate-buffered saline (PBS) and collected in TRIzol (15596; Thermo) for RNA extraction according to the manufacturer’s instructions or fixed with 4% formaldehyde for 10 min for quantification by microscopy. .. Cells were stained for immunofluorescence imaging using anti-CHIKV ascites V-548-701-562 (ATCC VR-1241AF) at 1:300 or CHK-265 (gift from M. Diamond) at 1:500 and anti-mouse 488 and Hoechst 33342 (B2261; Sigma-Aldrich) at a final concentration of 5 μg/ml. ..

    Article Title: Chikungunya Virus Strains from Each Genetic Clade Bind Sulfated Glycosaminoglycans as Attachment Factors
    Article Snippet: .. Blocking buffer (PBS+/+ supplemented to contain 5% FBS and 0.1% TX-100) was added to the plate at room temperature and left for 1 h. Cells were stained with anti-CHIKV ascites fluid (1:1,500; ATCC VR-1241AF) at room temperature for 1 h and with goat anti-mouse Alexa Fluor 488 IgG (1:1,000; Invitrogen A11029 ) with 4′,6-diamidino-2-phenylindole (DAPI) (1:1,000; Thermo Fisher Scientific) at room temperature for 1 h. Cells were washed with PBS−/− three times at room temperature for 5 min per wash between each staining step. .. Infectivity was quantified by indirect immunofluorescence using the Lionheart FX automated microscope and Gen5 software (BioTek).

    Article Title: A Single-Amino-Acid Polymorphism in Chikungunya Virus E2 Glycoprotein Influences Glycosaminoglycan Utilization
    Article Snippet: Infected cells were visualized using indirect immunofluorescence. .. Cells were incubated in blocking buffer (phosphate-buffered saline [PBS; Gibco] containing 5% FBS and 0.1% Triton X-100) at room temperature for 1 h and stained with precleared anti-CHIKV immune ascetic fluid (ATCC VR-1241AF) diluted 1:1,500 and secondary Alexa 488 goat anti-mouse antibody diluted 1:1,000 (Invitrogen), followed by addition of DAPI (4′,6-diamidino-2-phenylindole) to visualize cell nuclei. .. For some experiments, cells were visualized using an Axiovert 200 fluorescence microscope (Zeiss).

    Immunofluorescence:

    Article Title: DDX56 Binds to Chikungunya Virus RNA To Control Infection
    Article Snippet: At the indicated time points, cells were washed once with phosphate-buffered saline (PBS) and collected in TRIzol (15596; Thermo) for RNA extraction according to the manufacturer’s instructions or fixed with 4% formaldehyde for 10 min for quantification by microscopy. .. Cells were stained for immunofluorescence imaging using anti-CHIKV ascites V-548-701-562 (ATCC VR-1241AF) at 1:300 or CHK-265 (gift from M. Diamond) at 1:500 and anti-mouse 488 and Hoechst 33342 (B2261; Sigma-Aldrich) at a final concentration of 5 μg/ml. ..

    Article Title: DNA Vaccine Initiates Replication of Live Attenuated Chikungunya Virus In Vitro and Elicits Protective Immune Response in Mice
    Article Snippet: As controls, cells were incubated with 102 –105 plaque-forming units (PFUs) of CHIKV 181/25 vaccine virus. .. Expression of CHIKV antigens in iDNA-transfected and virus-infected cells was detected by immunofluorescence assay (IFA) and Western blot, using CHIKV hyperimmune mouse ascitic fluid (HMAF) VR-1241AF (ATCC). .. CHIKV antigens were also confirmed by Western blot, using convalescent human antiserum (UTMB; courtesy of Dr Robert Tesh).

    Imaging:

    Article Title: DDX56 Binds to Chikungunya Virus RNA To Control Infection
    Article Snippet: At the indicated time points, cells were washed once with phosphate-buffered saline (PBS) and collected in TRIzol (15596; Thermo) for RNA extraction according to the manufacturer’s instructions or fixed with 4% formaldehyde for 10 min for quantification by microscopy. .. Cells were stained for immunofluorescence imaging using anti-CHIKV ascites V-548-701-562 (ATCC VR-1241AF) at 1:300 or CHK-265 (gift from M. Diamond) at 1:500 and anti-mouse 488 and Hoechst 33342 (B2261; Sigma-Aldrich) at a final concentration of 5 μg/ml. ..

    Concentration Assay:

    Article Title: DDX56 Binds to Chikungunya Virus RNA To Control Infection
    Article Snippet: At the indicated time points, cells were washed once with phosphate-buffered saline (PBS) and collected in TRIzol (15596; Thermo) for RNA extraction according to the manufacturer’s instructions or fixed with 4% formaldehyde for 10 min for quantification by microscopy. .. Cells were stained for immunofluorescence imaging using anti-CHIKV ascites V-548-701-562 (ATCC VR-1241AF) at 1:300 or CHK-265 (gift from M. Diamond) at 1:500 and anti-mouse 488 and Hoechst 33342 (B2261; Sigma-Aldrich) at a final concentration of 5 μg/ml. ..

    Blocking Assay:

    Article Title: Chikungunya Virus Strains from Each Genetic Clade Bind Sulfated Glycosaminoglycans as Attachment Factors
    Article Snippet: .. Blocking buffer (PBS+/+ supplemented to contain 5% FBS and 0.1% TX-100) was added to the plate at room temperature and left for 1 h. Cells were stained with anti-CHIKV ascites fluid (1:1,500; ATCC VR-1241AF) at room temperature for 1 h and with goat anti-mouse Alexa Fluor 488 IgG (1:1,000; Invitrogen A11029 ) with 4′,6-diamidino-2-phenylindole (DAPI) (1:1,000; Thermo Fisher Scientific) at room temperature for 1 h. Cells were washed with PBS−/− three times at room temperature for 5 min per wash between each staining step. .. Infectivity was quantified by indirect immunofluorescence using the Lionheart FX automated microscope and Gen5 software (BioTek).

    Article Title: A Single-Amino-Acid Polymorphism in Chikungunya Virus E2 Glycoprotein Influences Glycosaminoglycan Utilization
    Article Snippet: Infected cells were visualized using indirect immunofluorescence. .. Cells were incubated in blocking buffer (phosphate-buffered saline [PBS; Gibco] containing 5% FBS and 0.1% Triton X-100) at room temperature for 1 h and stained with precleared anti-CHIKV immune ascetic fluid (ATCC VR-1241AF) diluted 1:1,500 and secondary Alexa 488 goat anti-mouse antibody diluted 1:1,000 (Invitrogen), followed by addition of DAPI (4′,6-diamidino-2-phenylindole) to visualize cell nuclei. .. For some experiments, cells were visualized using an Axiovert 200 fluorescence microscope (Zeiss).

    Expressing:

    Article Title: DNA Vaccine Initiates Replication of Live Attenuated Chikungunya Virus In Vitro and Elicits Protective Immune Response in Mice
    Article Snippet: As controls, cells were incubated with 102 –105 plaque-forming units (PFUs) of CHIKV 181/25 vaccine virus. .. Expression of CHIKV antigens in iDNA-transfected and virus-infected cells was detected by immunofluorescence assay (IFA) and Western blot, using CHIKV hyperimmune mouse ascitic fluid (HMAF) VR-1241AF (ATCC). .. CHIKV antigens were also confirmed by Western blot, using convalescent human antiserum (UTMB; courtesy of Dr Robert Tesh).

    Western Blot:

    Article Title: DNA Vaccine Initiates Replication of Live Attenuated Chikungunya Virus In Vitro and Elicits Protective Immune Response in Mice
    Article Snippet: As controls, cells were incubated with 102 –105 plaque-forming units (PFUs) of CHIKV 181/25 vaccine virus. .. Expression of CHIKV antigens in iDNA-transfected and virus-infected cells was detected by immunofluorescence assay (IFA) and Western blot, using CHIKV hyperimmune mouse ascitic fluid (HMAF) VR-1241AF (ATCC). .. CHIKV antigens were also confirmed by Western blot, using convalescent human antiserum (UTMB; courtesy of Dr Robert Tesh).

    Incubation:

    Article Title: A Single-Amino-Acid Polymorphism in Chikungunya Virus E2 Glycoprotein Influences Glycosaminoglycan Utilization
    Article Snippet: Infected cells were visualized using indirect immunofluorescence. .. Cells were incubated in blocking buffer (phosphate-buffered saline [PBS; Gibco] containing 5% FBS and 0.1% Triton X-100) at room temperature for 1 h and stained with precleared anti-CHIKV immune ascetic fluid (ATCC VR-1241AF) diluted 1:1,500 and secondary Alexa 488 goat anti-mouse antibody diluted 1:1,000 (Invitrogen), followed by addition of DAPI (4′,6-diamidino-2-phenylindole) to visualize cell nuclei. .. For some experiments, cells were visualized using an Axiovert 200 fluorescence microscope (Zeiss).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    ATCC chikv antigen
    Chikv Antigen, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chikv antigen/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chikv antigen - by Bioz Stars, 2021-05
    94/100 stars
      Buy from Supplier

    Image Search Results