chikv antigen  (ATCC)


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    Name:
    Chikungunya virus immune ascitic fluid V 548 701 562
    Description:

    Catalog Number:
    vr-1241af
    Price:
    None
    Source:
    Mouse
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    Structured Review

    ATCC chikv antigen

    https://www.bioz.com/result/chikv antigen/product/ATCC
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chikv antigen - by Bioz Stars, 2020-08
    90/100 stars

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    Related Articles

    Transfection:

    Article Title: Next Generation Sequencing of DNA-Launched Chikungunya Vaccine Virus
    Article Snippet: .. Aliquots of transfected cell suspension were taken for CHIKV expression tests by immunofluorescence assay (IFA), infectious center assay (ICA) and western blot using CHIKV hyperimmune mouse ascitic fluid (HMAF) VR-64 (ATCC VR-1241AF) prepared against Chikungunya strain S-27. .. For ICA, electroporated cells were seeded into 6-well plates, allowed to adhere for 4 h, covered with 1% low-melting agarose in complete medium (aMEM containing 10% FBS and 10 μg/ml gentamicin), and incubated for 72 h. To visualize plaques, monolayers were stained using neutral red and counted the following day.

    Blocking Assay:

    Article Title: A Single-Amino-Acid Polymorphism in Chikungunya Virus E2 Glycoprotein Influences Glycosaminoglycan Utilization
    Article Snippet: .. Cells were incubated in blocking buffer (phosphate-buffered saline [PBS; Gibco] containing 5% FBS and 0.1% Triton X-100) at room temperature for 1 h and stained with precleared anti-CHIKV immune ascetic fluid (ATCC VR-1241AF) diluted 1:1,500 and secondary Alexa 488 goat anti-mouse antibody diluted 1:1,000 (Invitrogen), followed by addition of DAPI (4′,6-diamidino-2-phenylindole) to visualize cell nuclei. .. For some experiments, cells were visualized using an Axiovert 200 fluorescence microscope (Zeiss).

    Incubation:

    Article Title: A Single-Amino-Acid Polymorphism in Chikungunya Virus E2 Glycoprotein Influences Glycosaminoglycan Utilization
    Article Snippet: .. Cells were incubated in blocking buffer (phosphate-buffered saline [PBS; Gibco] containing 5% FBS and 0.1% Triton X-100) at room temperature for 1 h and stained with precleared anti-CHIKV immune ascetic fluid (ATCC VR-1241AF) diluted 1:1,500 and secondary Alexa 488 goat anti-mouse antibody diluted 1:1,000 (Invitrogen), followed by addition of DAPI (4′,6-diamidino-2-phenylindole) to visualize cell nuclei. .. For some experiments, cells were visualized using an Axiovert 200 fluorescence microscope (Zeiss).

    Expressing:

    Article Title: DNA Vaccine Initiates Replication of Live Attenuated Chikungunya Virus In Vitro and Elicits Protective Immune Response in Mice
    Article Snippet: .. Expression of CHIKV antigens in iDNA-transfected and virus-infected cells was detected by immunofluorescence assay (IFA) and Western blot, using CHIKV hyperimmune mouse ascitic fluid (HMAF) VR-1241AF (ATCC). .. CHIKV antigens were also confirmed by Western blot, using convalescent human antiserum (UTMB; courtesy of Dr Robert Tesh).

    Article Title: Next Generation Sequencing of DNA-Launched Chikungunya Vaccine Virus
    Article Snippet: .. Aliquots of transfected cell suspension were taken for CHIKV expression tests by immunofluorescence assay (IFA), infectious center assay (ICA) and western blot using CHIKV hyperimmune mouse ascitic fluid (HMAF) VR-64 (ATCC VR-1241AF) prepared against Chikungunya strain S-27. .. For ICA, electroporated cells were seeded into 6-well plates, allowed to adhere for 4 h, covered with 1% low-melting agarose in complete medium (aMEM containing 10% FBS and 10 μg/ml gentamicin), and incubated for 72 h. To visualize plaques, monolayers were stained using neutral red and counted the following day.

    Western Blot:

    Article Title: DNA Vaccine Initiates Replication of Live Attenuated Chikungunya Virus In Vitro and Elicits Protective Immune Response in Mice
    Article Snippet: .. Expression of CHIKV antigens in iDNA-transfected and virus-infected cells was detected by immunofluorescence assay (IFA) and Western blot, using CHIKV hyperimmune mouse ascitic fluid (HMAF) VR-1241AF (ATCC). .. CHIKV antigens were also confirmed by Western blot, using convalescent human antiserum (UTMB; courtesy of Dr Robert Tesh).

    Article Title: Next Generation Sequencing of DNA-Launched Chikungunya Vaccine Virus
    Article Snippet: .. Aliquots of transfected cell suspension were taken for CHIKV expression tests by immunofluorescence assay (IFA), infectious center assay (ICA) and western blot using CHIKV hyperimmune mouse ascitic fluid (HMAF) VR-64 (ATCC VR-1241AF) prepared against Chikungunya strain S-27. .. For ICA, electroporated cells were seeded into 6-well plates, allowed to adhere for 4 h, covered with 1% low-melting agarose in complete medium (aMEM containing 10% FBS and 10 μg/ml gentamicin), and incubated for 72 h. To visualize plaques, monolayers were stained using neutral red and counted the following day.

    Staining:

    Article Title: A Single-Amino-Acid Polymorphism in Chikungunya Virus E2 Glycoprotein Influences Glycosaminoglycan Utilization
    Article Snippet: .. Cells were incubated in blocking buffer (phosphate-buffered saline [PBS; Gibco] containing 5% FBS and 0.1% Triton X-100) at room temperature for 1 h and stained with precleared anti-CHIKV immune ascetic fluid (ATCC VR-1241AF) diluted 1:1,500 and secondary Alexa 488 goat anti-mouse antibody diluted 1:1,000 (Invitrogen), followed by addition of DAPI (4′,6-diamidino-2-phenylindole) to visualize cell nuclei. .. For some experiments, cells were visualized using an Axiovert 200 fluorescence microscope (Zeiss).

    Immunofluorescence:

    Article Title: DNA Vaccine Initiates Replication of Live Attenuated Chikungunya Virus In Vitro and Elicits Protective Immune Response in Mice
    Article Snippet: .. Expression of CHIKV antigens in iDNA-transfected and virus-infected cells was detected by immunofluorescence assay (IFA) and Western blot, using CHIKV hyperimmune mouse ascitic fluid (HMAF) VR-1241AF (ATCC). .. CHIKV antigens were also confirmed by Western blot, using convalescent human antiserum (UTMB; courtesy of Dr Robert Tesh).

    Article Title: Next Generation Sequencing of DNA-Launched Chikungunya Vaccine Virus
    Article Snippet: .. Aliquots of transfected cell suspension were taken for CHIKV expression tests by immunofluorescence assay (IFA), infectious center assay (ICA) and western blot using CHIKV hyperimmune mouse ascitic fluid (HMAF) VR-64 (ATCC VR-1241AF) prepared against Chikungunya strain S-27. .. For ICA, electroporated cells were seeded into 6-well plates, allowed to adhere for 4 h, covered with 1% low-melting agarose in complete medium (aMEM containing 10% FBS and 10 μg/ml gentamicin), and incubated for 72 h. To visualize plaques, monolayers were stained using neutral red and counted the following day.

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  • 86
    ATCC chikv antigens
    Transfection of immunization DNA <t>(iDNA)</t> plasmid p181/25-7 into CHO cells. A , The immunization DNA (iDNA) approach for launching chikungunya virus <t>(CHIKV)</t> live attenuated virus in eukaryotic cells is shown on the left. Indicated are the cytomegalovirus (CMV) promoter (open arrow), cell nucleus, and progeny virus. Expression of CHIKV antigens after transfection of iDNA plasmid is shown on the right, detected by immunofluorescence assay (IFA) 48 and 96 hours after transfection. Aliquots of transfected cells were seeded in 8-well chamber slides, fixed at indicated times in cold acetone, and processed by IFA, using mouse CHIKV-specific antibody, followed by fluorescein isothiocyanate–conjugated secondary antibody. B , Detection of CHIKV antigens in transfected CHO cells by Western blot (left) and in the growth medium by plaque assay 48 hours after transfection (middle). For comparison, plaque assay for the 181/25 virus vaccine (passage 1 in CHO cells) is shown. Right panel shows growth curve of p181/25-7 iDNA-derived virus (average of 3 experiments). Western blot was done using human convalescent-phase CHIKV-specific serum (lane 1) and CHIKV HMAF (lane 2). The PE2, E2, E1, and C antigens are indicated.
    Chikv Antigens, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chikv antigens/product/ATCC
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    chikv antigens - by Bioz Stars, 2020-08
    86/100 stars
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    Transfection of immunization DNA (iDNA) plasmid p181/25-7 into CHO cells. A , The immunization DNA (iDNA) approach for launching chikungunya virus (CHIKV) live attenuated virus in eukaryotic cells is shown on the left. Indicated are the cytomegalovirus (CMV) promoter (open arrow), cell nucleus, and progeny virus. Expression of CHIKV antigens after transfection of iDNA plasmid is shown on the right, detected by immunofluorescence assay (IFA) 48 and 96 hours after transfection. Aliquots of transfected cells were seeded in 8-well chamber slides, fixed at indicated times in cold acetone, and processed by IFA, using mouse CHIKV-specific antibody, followed by fluorescein isothiocyanate–conjugated secondary antibody. B , Detection of CHIKV antigens in transfected CHO cells by Western blot (left) and in the growth medium by plaque assay 48 hours after transfection (middle). For comparison, plaque assay for the 181/25 virus vaccine (passage 1 in CHO cells) is shown. Right panel shows growth curve of p181/25-7 iDNA-derived virus (average of 3 experiments). Western blot was done using human convalescent-phase CHIKV-specific serum (lane 1) and CHIKV HMAF (lane 2). The PE2, E2, E1, and C antigens are indicated.

    Journal: The Journal of Infectious Diseases

    Article Title: DNA Vaccine Initiates Replication of Live Attenuated Chikungunya Virus In Vitro and Elicits Protective Immune Response in Mice

    doi: 10.1093/infdis/jiu114

    Figure Lengend Snippet: Transfection of immunization DNA (iDNA) plasmid p181/25-7 into CHO cells. A , The immunization DNA (iDNA) approach for launching chikungunya virus (CHIKV) live attenuated virus in eukaryotic cells is shown on the left. Indicated are the cytomegalovirus (CMV) promoter (open arrow), cell nucleus, and progeny virus. Expression of CHIKV antigens after transfection of iDNA plasmid is shown on the right, detected by immunofluorescence assay (IFA) 48 and 96 hours after transfection. Aliquots of transfected cells were seeded in 8-well chamber slides, fixed at indicated times in cold acetone, and processed by IFA, using mouse CHIKV-specific antibody, followed by fluorescein isothiocyanate–conjugated secondary antibody. B , Detection of CHIKV antigens in transfected CHO cells by Western blot (left) and in the growth medium by plaque assay 48 hours after transfection (middle). For comparison, plaque assay for the 181/25 virus vaccine (passage 1 in CHO cells) is shown. Right panel shows growth curve of p181/25-7 iDNA-derived virus (average of 3 experiments). Western blot was done using human convalescent-phase CHIKV-specific serum (lane 1) and CHIKV HMAF (lane 2). The PE2, E2, E1, and C antigens are indicated.

    Article Snippet: Expression of CHIKV antigens in iDNA-transfected and virus-infected cells was detected by immunofluorescence assay (IFA) and Western blot, using CHIKV hyperimmune mouse ascitic fluid (HMAF) VR-1241AF (ATCC).

    Techniques: Transfection, Plasmid Preparation, Expressing, Immunofluorescence, Western Blot, Plaque Assay, Derivative Assay