Structured Review

Bio-Rad chemidoc xrs system
Selective labeling of sfGFPs that contained site-specifically incorporated NCAAs with strained alkene/alkyne functionalities with (A) FITC-TZ and (B) HZCL. In A and B, the top panels show denaturing SDS-PAGE analysis of proteins stained with Coomassie blue and the bottom panels are from fluorescent imaging of the same gels before Coomassie blue staining. In A, the fluorescent image was captured by a digital camera and displayed was real color of the emitting light. In B, the fluorescent image was captured by a Bio-Rad <t>ChemiDoc</t> <t>XRS</t> system.
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Images

1) Product Images from "Two Rapid Catalyst-Free Click Reactions for In Vivo Protein Labeling of Genetically Encoded Strained Alkene/Alkyne Functionalities"

Article Title: Two Rapid Catalyst-Free Click Reactions for In Vivo Protein Labeling of Genetically Encoded Strained Alkene/Alkyne Functionalities

Journal: Bioconjugate Chemistry

doi: 10.1021/bc500361d

Selective labeling of sfGFPs that contained site-specifically incorporated NCAAs with strained alkene/alkyne functionalities with (A) FITC-TZ and (B) HZCL. In A and B, the top panels show denaturing SDS-PAGE analysis of proteins stained with Coomassie blue and the bottom panels are from fluorescent imaging of the same gels before Coomassie blue staining. In A, the fluorescent image was captured by a digital camera and displayed was real color of the emitting light. In B, the fluorescent image was captured by a Bio-Rad ChemiDoc XRS system.
Figure Legend Snippet: Selective labeling of sfGFPs that contained site-specifically incorporated NCAAs with strained alkene/alkyne functionalities with (A) FITC-TZ and (B) HZCL. In A and B, the top panels show denaturing SDS-PAGE analysis of proteins stained with Coomassie blue and the bottom panels are from fluorescent imaging of the same gels before Coomassie blue staining. In A, the fluorescent image was captured by a digital camera and displayed was real color of the emitting light. In B, the fluorescent image was captured by a Bio-Rad ChemiDoc XRS system.

Techniques Used: Labeling, SDS Page, Staining, Imaging

2) Product Images from "Viral fusion efficacy of specific H3N2 influenza virus reassortant combinations at single-particle level"

Article Title: Viral fusion efficacy of specific H3N2 influenza virus reassortant combinations at single-particle level

Journal: Scientific Reports

doi: 10.1038/srep35537

Pseudotyped particle protein density analysis. Quantification of intensities of HA, NA and VSV M protein by Western Blot ( A ). The HA 1 ( B ) and NA ( C ) band intensities were directly detected by Chemidoc XRS+ imager and normalized to corresponding M band intensity to obtain the relative HA and NA expression levels on different VSV pseudotyped particles. Each error bar represents the mean ± SD of three independent experiments. NA protein was also detected except for HA only particles.
Figure Legend Snippet: Pseudotyped particle protein density analysis. Quantification of intensities of HA, NA and VSV M protein by Western Blot ( A ). The HA 1 ( B ) and NA ( C ) band intensities were directly detected by Chemidoc XRS+ imager and normalized to corresponding M band intensity to obtain the relative HA and NA expression levels on different VSV pseudotyped particles. Each error bar represents the mean ± SD of three independent experiments. NA protein was also detected except for HA only particles.

Techniques Used: Western Blot, Expressing

3) Product Images from "Muscle Tissue Damage Induced by the Venom of Bothrops asper: Identification of Early and Late Pathological Events through Proteomic Analysis"

Article Title: Muscle Tissue Damage Induced by the Venom of Bothrops asper: Identification of Early and Late Pathological Events through Proteomic Analysis

Journal: PLoS Neglected Tropical Diseases

doi: 10.1371/journal.pntd.0004599

Western blot analysis of extracellular matrix components in wound exudates collected from mice after injection of B . asper venom. Groups of five mice were injected in the gastrocnemius with 50 μg of B . asper venom. After 1, 6 and 24 h of injection mice were sacrificed and samples of exudates were collected, pooled and lyophilized. Afterwards, 100 μg of protein of each sample were separated under reducing conditions on 4–15% Tris–HCl SDS-PAGE, and transferred to nitrocellulose membranes. Immunodetection was performed with (A) anti-type IV collagen (Col IV), (B) anti-laminin, (C) anti-nidogen 1, (D) anti-type I collagen (Col I), (E) anti-type VI collagen (Col VI), and (F) anti-fibronectin. The reaction was detected using an anti-rabbit peroxidase antibody and a chemiluminescent substrate. Images were obtained with the ChemiDoc XRS+ System (BioRad).
Figure Legend Snippet: Western blot analysis of extracellular matrix components in wound exudates collected from mice after injection of B . asper venom. Groups of five mice were injected in the gastrocnemius with 50 μg of B . asper venom. After 1, 6 and 24 h of injection mice were sacrificed and samples of exudates were collected, pooled and lyophilized. Afterwards, 100 μg of protein of each sample were separated under reducing conditions on 4–15% Tris–HCl SDS-PAGE, and transferred to nitrocellulose membranes. Immunodetection was performed with (A) anti-type IV collagen (Col IV), (B) anti-laminin, (C) anti-nidogen 1, (D) anti-type I collagen (Col I), (E) anti-type VI collagen (Col VI), and (F) anti-fibronectin. The reaction was detected using an anti-rabbit peroxidase antibody and a chemiluminescent substrate. Images were obtained with the ChemiDoc XRS+ System (BioRad).

Techniques Used: Western Blot, Mouse Assay, Injection, SDS Page, Immunodetection

4) Product Images from "Bacterial periplasmic nitrate and trimethylamine-N-oxide respiration coupled to menaquinol-cytochrome c reductase (Qcr): Implications for electrogenic reduction of alternative electron acceptors"

Article Title: Bacterial periplasmic nitrate and trimethylamine-N-oxide respiration coupled to menaquinol-cytochrome c reductase (Qcr): Implications for electrogenic reduction of alternative electron acceptors

Journal: Scientific Reports

doi: 10.1038/s41598-018-33857-2

Construction and verification of a qcrABC deletion mutant. ( A ) Mutagenesis strategy. The majority of the coding regions of the qcrA-C genes were replaced with a kanamycin resistance cassette with its own promoter. The downstream cfa gene has its own promoter, but the cassette has no terminator and so should be non-polar on cfa . ( B ) RT-PCR to verify expression of cfa gene and absence of qcr gene transcription in the qcrABC mutant. Fold-change in the mutant is shown relative to the wild type strain, using gyrA gene as a control. The difference between expression of the cfa gene in mutant and wild-type was not significant by t-test. ( C ) shows a comparison of the membrane associated c -type cytochromes revealed after a total membrane preparation was subjected to SDS-PAGE and electroblotting to a nitrocellulose membrane, followed by staining for haem-associated peroxidase activity using the chemiluminescence technique described in Methods. 20 μg total protein was run per lane. The Image shown is a cropped version of the full-size blot that can viewed in Supplementary Fig. 1 , and was obtained using a ChemiDoc XRS system (BioRad Inc) with an exposure time of 2 min. A band corresponding to the expected size of QcrC is missing in the qcrABC deletion mutant.
Figure Legend Snippet: Construction and verification of a qcrABC deletion mutant. ( A ) Mutagenesis strategy. The majority of the coding regions of the qcrA-C genes were replaced with a kanamycin resistance cassette with its own promoter. The downstream cfa gene has its own promoter, but the cassette has no terminator and so should be non-polar on cfa . ( B ) RT-PCR to verify expression of cfa gene and absence of qcr gene transcription in the qcrABC mutant. Fold-change in the mutant is shown relative to the wild type strain, using gyrA gene as a control. The difference between expression of the cfa gene in mutant and wild-type was not significant by t-test. ( C ) shows a comparison of the membrane associated c -type cytochromes revealed after a total membrane preparation was subjected to SDS-PAGE and electroblotting to a nitrocellulose membrane, followed by staining for haem-associated peroxidase activity using the chemiluminescence technique described in Methods. 20 μg total protein was run per lane. The Image shown is a cropped version of the full-size blot that can viewed in Supplementary Fig. 1 , and was obtained using a ChemiDoc XRS system (BioRad Inc) with an exposure time of 2 min. A band corresponding to the expected size of QcrC is missing in the qcrABC deletion mutant.

Techniques Used: Mutagenesis, Reverse Transcription Polymerase Chain Reaction, Expressing, SDS Page, Staining, Activity Assay

5) Product Images from "Rapid Regeneration and Reuse of Silica Columns from PCR Purification and Gel Extraction Kits"

Article Title: Rapid Regeneration and Reuse of Silica Columns from PCR Purification and Gel Extraction Kits

Journal: Scientific Reports

doi: 10.1038/s41598-018-30316-w

Regenerated PCR purification kit columns have a comparable capacity for DNA purification as fresh columns. ( A ) A scheme showing the workflow and duration for the published regeneration methods and for the 1 M phosphoric acid method. PM: published method. ( B ) Comparison of DNA elimination using 1 M phosphoric acid and the published methods. The used columns were cleaned with the protocol as shown in A. Thirty microlitres of eluate was obtained from the regenerated columns, 1 μL of which was used for qPCR. The data were processed as shown in Fig. 1C . ( C ) Comparison of DNA purification efficacy between the regenerated and fresh columns. A 50 μL Tbox 5 PCR reaction was purified with the regenerated or fresh columns, and the DNA concentrations were measured with a Nanophotometer (Thermo Scientific) and then subjected to a statistical analysis. The DNA quality was verified by agarose gel electrophoresis and imaged using a Bio-Rad ChemiDoc XRS + System. Data acquisition and settings are described as in the materials and methods section. The full-length image is presented in Supplementary Fig. S2 . The DNA concentration for each sample is shown beneath the gel picture. ( D ) The regenerated columns showed a similar capacity for purifying different sizes of DNA as the fresh columns. DNA from a 50 μL PCR reaction was purified and quantified as in C. Luc shRNA, Luciferase shRNA cDNA, 70 bp; Hand2, 654 bp, Tbox 5, 1557 bp; pLV-FLNB, 17360 bp. ( E ) Comparison of DNA purification efficacy between the regenerated and fresh columns from different PCR purification kits. DNA from a 50 μL LIF PCR reaction was purified using regenerated or fresh columns, and the data were processed and presented as in C. Each column in B and D represents the mean ± SD of three independent experiments. * p
Figure Legend Snippet: Regenerated PCR purification kit columns have a comparable capacity for DNA purification as fresh columns. ( A ) A scheme showing the workflow and duration for the published regeneration methods and for the 1 M phosphoric acid method. PM: published method. ( B ) Comparison of DNA elimination using 1 M phosphoric acid and the published methods. The used columns were cleaned with the protocol as shown in A. Thirty microlitres of eluate was obtained from the regenerated columns, 1 μL of which was used for qPCR. The data were processed as shown in Fig. 1C . ( C ) Comparison of DNA purification efficacy between the regenerated and fresh columns. A 50 μL Tbox 5 PCR reaction was purified with the regenerated or fresh columns, and the DNA concentrations were measured with a Nanophotometer (Thermo Scientific) and then subjected to a statistical analysis. The DNA quality was verified by agarose gel electrophoresis and imaged using a Bio-Rad ChemiDoc XRS + System. Data acquisition and settings are described as in the materials and methods section. The full-length image is presented in Supplementary Fig. S2 . The DNA concentration for each sample is shown beneath the gel picture. ( D ) The regenerated columns showed a similar capacity for purifying different sizes of DNA as the fresh columns. DNA from a 50 μL PCR reaction was purified and quantified as in C. Luc shRNA, Luciferase shRNA cDNA, 70 bp; Hand2, 654 bp, Tbox 5, 1557 bp; pLV-FLNB, 17360 bp. ( E ) Comparison of DNA purification efficacy between the regenerated and fresh columns from different PCR purification kits. DNA from a 50 μL LIF PCR reaction was purified using regenerated or fresh columns, and the data were processed and presented as in C. Each column in B and D represents the mean ± SD of three independent experiments. * p

Techniques Used: Polymerase Chain Reaction, Purification, DNA Purification, Real-time Polymerase Chain Reaction, Agarose Gel Electrophoresis, Concentration Assay, shRNA, Luciferase

6) Product Images from "Abrogation of Neuraminidase Reduces Biofilm Formation, Capsule Biosynthesis, and Virulence of Porphyromonas gingivalis"

Article Title: Abrogation of Neuraminidase Reduces Biofilm Formation, Capsule Biosynthesis, and Virulence of Porphyromonas gingivalis

Journal: Infection and Immunity

doi: 10.1128/IAI.05773-11

PG0352 (Sia Pg ) exhibits neuraminidase activity. (A) Preparation of recombinant PG0352 protein (rSia Pg ). (B) Filter paper spot test of rSia Pg . The assay was conducted using 4-MUNANA as the substrate, and the images were processed using the ChemiDoc XRS
Figure Legend Snippet: PG0352 (Sia Pg ) exhibits neuraminidase activity. (A) Preparation of recombinant PG0352 protein (rSia Pg ). (B) Filter paper spot test of rSia Pg . The assay was conducted using 4-MUNANA as the substrate, and the images were processed using the ChemiDoc XRS

Techniques Used: Activity Assay, Recombinant, Spot Test

7) Product Images from "Development of viral nanoparticles for efficient intracellular delivery †"

Article Title: Development of viral nanoparticles for efficient intracellular delivery †

Journal: Nanoscale

doi: 10.1039/c2nr30366c

Characterization of CPMV labeling with the biotinylated R5 peptide. (A) Size exclusion chromatography of wild-type CPMV, CPMV–R5L and CPMV–R5Hat 280 nm. (B) ECL dot blot of purified CPMV particles. The number of biotin labels per particle was determined using standardized biotin concentrations and Chemidoc XRS software. (C) Native gel electrophoresis of intact CPMV particles (10 µg) using a 0.8% (w/v) agarose gel. Particles were visualized under UV light. Lane 1 = CPMV, 2 = CPMV–4FB, 3 = CPMV–R5H, 4 = CPMV–PFB, 5 = CPMV–R5L. (D) SDS–PAGE of CPMV particles (10 µg) using a 4–12% Bis-Tris gel and western blotting using streptavidin–alkaline phosphatase to detect the N-terminal biotin tag of the R5 peptide. (E) Zeta potential of CPMV wild type, CPMV–R5L and CPMV–R5H formulations.
Figure Legend Snippet: Characterization of CPMV labeling with the biotinylated R5 peptide. (A) Size exclusion chromatography of wild-type CPMV, CPMV–R5L and CPMV–R5Hat 280 nm. (B) ECL dot blot of purified CPMV particles. The number of biotin labels per particle was determined using standardized biotin concentrations and Chemidoc XRS software. (C) Native gel electrophoresis of intact CPMV particles (10 µg) using a 0.8% (w/v) agarose gel. Particles were visualized under UV light. Lane 1 = CPMV, 2 = CPMV–4FB, 3 = CPMV–R5H, 4 = CPMV–PFB, 5 = CPMV–R5L. (D) SDS–PAGE of CPMV particles (10 µg) using a 4–12% Bis-Tris gel and western blotting using streptavidin–alkaline phosphatase to detect the N-terminal biotin tag of the R5 peptide. (E) Zeta potential of CPMV wild type, CPMV–R5L and CPMV–R5H formulations.

Techniques Used: Labeling, Size-exclusion Chromatography, Dot Blot, Purification, Software, Nucleic Acid Electrophoresis, Agarose Gel Electrophoresis, SDS Page, Western Blot

8) Product Images from "Identification of aminosulfonylarylisoxazole as microRNA-31 regulators"

Article Title: Identification of aminosulfonylarylisoxazole as microRNA-31 regulators

Journal: PLoS ONE

doi: 10.1371/journal.pone.0182331

Protein expression of miR-31 targets following compound treatment. A549 cells were treated with compounds 2, 3, and 4 and lysed with RIPA buffer. Total protein concentration was calculated using a BCA assay kit, and 20 μg of total protein was used for western blot analyses of PPP2R2A (A), E2F2 (B), and STK40 (C). The intensity of each blot was normalized against that of β-tubulin. Bands were detected using the ChemiDoc™ XRS+ System. (* represents non-specific band).
Figure Legend Snippet: Protein expression of miR-31 targets following compound treatment. A549 cells were treated with compounds 2, 3, and 4 and lysed with RIPA buffer. Total protein concentration was calculated using a BCA assay kit, and 20 μg of total protein was used for western blot analyses of PPP2R2A (A), E2F2 (B), and STK40 (C). The intensity of each blot was normalized against that of β-tubulin. Bands were detected using the ChemiDoc™ XRS+ System. (* represents non-specific band).

Techniques Used: Expressing, Protein Concentration, BIA-KA, Western Blot

9) Product Images from "Tissue Localization and Extracellular Matrix Degradation by PI, PII and PIII Snake Venom Metalloproteinases: Clues on the Mechanisms of Venom-Induced Hemorrhage"

Article Title: Tissue Localization and Extracellular Matrix Degradation by PI, PII and PIII Snake Venom Metalloproteinases: Clues on the Mechanisms of Venom-Induced Hemorrhage

Journal: PLoS Neglected Tropical Diseases

doi: 10.1371/journal.pntd.0003731

Western blot analysis of basement membrane components in skin homogenates. Groups of five mice were injected by intradermal route in the ventral abdominal region with either BaP1 (PI, 75 μg), BlatH1 (PII, 1.5 μg), CsH1 (PIII, 35 μg) SVMPs or PBS (lane C). After 15 min, mice were sacrificed, their skin was removed, and an area of 12 mm diameter was dissected out. Tissues of the same group were homogenized and centrifuged, and the supernatant collected. Then, 10–20 μL of each skin homogenate sample were separated under reducing conditions on 4–15% Tris–HCl SDS-PAGE gradient gels, and transferred to nitrocellulose membranes. Immunodetection was performed with (A) anti-collagen type IV, (B) anti-collagen type VI, (C) anti-laminin, and (D) anti-nidogen 1. The anti-GAPDH antibody was used as loading control. The reaction was detected using an anti-rabbit peroxidase antibody and a chemiluminescent substrate. Images were obtained with the ChemiDoc XRS+ System (BioRad).
Figure Legend Snippet: Western blot analysis of basement membrane components in skin homogenates. Groups of five mice were injected by intradermal route in the ventral abdominal region with either BaP1 (PI, 75 μg), BlatH1 (PII, 1.5 μg), CsH1 (PIII, 35 μg) SVMPs or PBS (lane C). After 15 min, mice were sacrificed, their skin was removed, and an area of 12 mm diameter was dissected out. Tissues of the same group were homogenized and centrifuged, and the supernatant collected. Then, 10–20 μL of each skin homogenate sample were separated under reducing conditions on 4–15% Tris–HCl SDS-PAGE gradient gels, and transferred to nitrocellulose membranes. Immunodetection was performed with (A) anti-collagen type IV, (B) anti-collagen type VI, (C) anti-laminin, and (D) anti-nidogen 1. The anti-GAPDH antibody was used as loading control. The reaction was detected using an anti-rabbit peroxidase antibody and a chemiluminescent substrate. Images were obtained with the ChemiDoc XRS+ System (BioRad).

Techniques Used: Western Blot, Mouse Assay, Injection, SDS Page, Immunodetection

Western blot analysis of basement membrane components in exudates collected from the gastrocnemius. Groups of five mice were injected in the right gastrocnemius with either BaP1 (PI, 75 μg), BlatH1 (PII, 3 μg), or CsH1 (PIII, 50 μg) SVMPs. After 15 min, mice were sacrificed, a 5 mm incision was made in the skin overlying the injected muscle, and a heparinized capillary tube was introduced under the skin to collect the wound exudate fluid; exudate samples from a single treatment were then pooled. Afterwards, 100 μg of protein of each sample was separated under reducing conditions on 4–15% Tris–HCl SDS-PAGE, and transferred to nitrocellulose membranes. Immunodetection was performed with (A) anti-collagen type IV, (B) anti-collagen type VI, (C) anti-laminin, and (D) anti-nidogen 1. The reaction was detected using an anti-rabbit peroxidase antibody and a chemiluminescent substrate. Images were obtained with the ChemiDoc XRS+ System (BioRad).
Figure Legend Snippet: Western blot analysis of basement membrane components in exudates collected from the gastrocnemius. Groups of five mice were injected in the right gastrocnemius with either BaP1 (PI, 75 μg), BlatH1 (PII, 3 μg), or CsH1 (PIII, 50 μg) SVMPs. After 15 min, mice were sacrificed, a 5 mm incision was made in the skin overlying the injected muscle, and a heparinized capillary tube was introduced under the skin to collect the wound exudate fluid; exudate samples from a single treatment were then pooled. Afterwards, 100 μg of protein of each sample was separated under reducing conditions on 4–15% Tris–HCl SDS-PAGE, and transferred to nitrocellulose membranes. Immunodetection was performed with (A) anti-collagen type IV, (B) anti-collagen type VI, (C) anti-laminin, and (D) anti-nidogen 1. The reaction was detected using an anti-rabbit peroxidase antibody and a chemiluminescent substrate. Images were obtained with the ChemiDoc XRS+ System (BioRad).

Techniques Used: Western Blot, Mouse Assay, Injection, SDS Page, Immunodetection

10) Product Images from "Conditional U1 Gene Silencing in Toxoplasma gondii"

Article Title: Conditional U1 Gene Silencing in Toxoplasma gondii

Journal: PLoS ONE

doi: 10.1371/journal.pone.0130356

The U1 mediated knockdown strategy is not functional in P . falciparum . (A) Schematic showing the strategy used to replace the endogenous pfsub1 3’ UTR with a floxed heterologous PbDT 3’ UTR with a downstream sequence comprising 10 U1 recognition sequences. The single cross-over homologous recombination event also results in the fusion of a HA3 epitope tag to PfSUB1. The predicted results of DiCre mediated recombination induced by treatment with rapamycin are shown. The positions of hybridization of PCR primers designed to specifically amplify sequences from the intact and excised locus are indicated with red and blue arrows. (B) IFA of mature schizonts of integrant clone D3, probed with mAb NIMP.M7 (anti PfSUB1) and mAb 3F10 (anti HA3). (C) Efficient DiCre-mediated recombination between the integrated loxP sites, as shown by diagnostic PCR analysis of parasite genomic DNA extracted 44 h following rapamycin treatment of two independent parasite clones D3 and B11. The blue and red arrows correspond to primer pairs marked in (A). (D) Upper panel: Western blot analysis with mAb 3F10 shows no down-regulation of PfSUB1 protein expression protein 44 h or 6 days after rapamycin treatment. Lower panel: loading controls, showing total protein SDS PAGE gels detected by UV fluorescence on the BioRad Chemidoc XRS system prior to Western transfer.
Figure Legend Snippet: The U1 mediated knockdown strategy is not functional in P . falciparum . (A) Schematic showing the strategy used to replace the endogenous pfsub1 3’ UTR with a floxed heterologous PbDT 3’ UTR with a downstream sequence comprising 10 U1 recognition sequences. The single cross-over homologous recombination event also results in the fusion of a HA3 epitope tag to PfSUB1. The predicted results of DiCre mediated recombination induced by treatment with rapamycin are shown. The positions of hybridization of PCR primers designed to specifically amplify sequences from the intact and excised locus are indicated with red and blue arrows. (B) IFA of mature schizonts of integrant clone D3, probed with mAb NIMP.M7 (anti PfSUB1) and mAb 3F10 (anti HA3). (C) Efficient DiCre-mediated recombination between the integrated loxP sites, as shown by diagnostic PCR analysis of parasite genomic DNA extracted 44 h following rapamycin treatment of two independent parasite clones D3 and B11. The blue and red arrows correspond to primer pairs marked in (A). (D) Upper panel: Western blot analysis with mAb 3F10 shows no down-regulation of PfSUB1 protein expression protein 44 h or 6 days after rapamycin treatment. Lower panel: loading controls, showing total protein SDS PAGE gels detected by UV fluorescence on the BioRad Chemidoc XRS system prior to Western transfer.

Techniques Used: Functional Assay, Sequencing, Homologous Recombination, Hybridization, Polymerase Chain Reaction, Immunofluorescence, Diagnostic Assay, Clone Assay, Western Blot, Expressing, SDS Page, Fluorescence

11) Product Images from "Conditional U1 Gene Silencing in Toxoplasma gondii"

Article Title: Conditional U1 Gene Silencing in Toxoplasma gondii

Journal: PLoS ONE

doi: 10.1371/journal.pone.0130356

The U1 mediated knockdown strategy is not functional in P . falciparum . (A) Schematic showing the strategy used to replace the endogenous pfsub1 3’ UTR with a floxed heterologous PbDT 3’ UTR with a downstream sequence comprising 10 U1 recognition sequences. The single cross-over homologous recombination event also results in the fusion of a HA3 epitope tag to PfSUB1. The predicted results of DiCre mediated recombination induced by treatment with rapamycin are shown. The positions of hybridization of PCR primers designed to specifically amplify sequences from the intact and excised locus are indicated with red and blue arrows. (B) IFA of mature schizonts of integrant clone D3, probed with mAb NIMP.M7 (anti PfSUB1) and mAb 3F10 (anti HA3). (C) Efficient DiCre-mediated recombination between the integrated loxP sites, as shown by diagnostic PCR analysis of parasite genomic DNA extracted 44 h following rapamycin treatment of two independent parasite clones D3 and B11. The blue and red arrows correspond to primer pairs marked in (A). (D) Upper panel: Western blot analysis with mAb 3F10 shows no down-regulation of PfSUB1 protein expression protein 44 h or 6 days after rapamycin treatment. Lower panel: loading controls, showing total protein SDS PAGE gels detected by UV fluorescence on the BioRad Chemidoc XRS system prior to Western transfer.
Figure Legend Snippet: The U1 mediated knockdown strategy is not functional in P . falciparum . (A) Schematic showing the strategy used to replace the endogenous pfsub1 3’ UTR with a floxed heterologous PbDT 3’ UTR with a downstream sequence comprising 10 U1 recognition sequences. The single cross-over homologous recombination event also results in the fusion of a HA3 epitope tag to PfSUB1. The predicted results of DiCre mediated recombination induced by treatment with rapamycin are shown. The positions of hybridization of PCR primers designed to specifically amplify sequences from the intact and excised locus are indicated with red and blue arrows. (B) IFA of mature schizonts of integrant clone D3, probed with mAb NIMP.M7 (anti PfSUB1) and mAb 3F10 (anti HA3). (C) Efficient DiCre-mediated recombination between the integrated loxP sites, as shown by diagnostic PCR analysis of parasite genomic DNA extracted 44 h following rapamycin treatment of two independent parasite clones D3 and B11. The blue and red arrows correspond to primer pairs marked in (A). (D) Upper panel: Western blot analysis with mAb 3F10 shows no down-regulation of PfSUB1 protein expression protein 44 h or 6 days after rapamycin treatment. Lower panel: loading controls, showing total protein SDS PAGE gels detected by UV fluorescence on the BioRad Chemidoc XRS system prior to Western transfer.

Techniques Used: Functional Assay, Sequencing, Homologous Recombination, Hybridization, Polymerase Chain Reaction, Immunofluorescence, Diagnostic Assay, Clone Assay, Western Blot, Expressing, SDS Page, Fluorescence

12) Product Images from "A novel bi-domain plant defensin MtDef5 with potent broad-spectrum antifungal activity binds to multiple phospholipids and forms oligomers"

Article Title: A novel bi-domain plant defensin MtDef5 with potent broad-spectrum antifungal activity binds to multiple phospholipids and forms oligomers

Journal: Scientific Reports

doi: 10.1038/s41598-017-16508-w

MtDef5 forms oligomers only in presence of PIP. ( A ) MtDef5 forms higher order oligomers in presence of PI3P, PI4P and PI5P as revealed by a protein cross-linking with BS 3 followed by SDS-PAGE and visualized using a Bio-Rad ChemiDoc XRS + system. ( B ) MtDef5 also forms oligomers in presence of PA and PI. The full length gel is presented in Supplementary Figure S5 .
Figure Legend Snippet: MtDef5 forms oligomers only in presence of PIP. ( A ) MtDef5 forms higher order oligomers in presence of PI3P, PI4P and PI5P as revealed by a protein cross-linking with BS 3 followed by SDS-PAGE and visualized using a Bio-Rad ChemiDoc XRS + system. ( B ) MtDef5 also forms oligomers in presence of PA and PI. The full length gel is presented in Supplementary Figure S5 .

Techniques Used: SDS Page

13) Product Images from "Giardia duodenalis Cathepsin B Proteases Degrade Intestinal Epithelial Interleukin-8 and Attenuate Interleukin-8-Induced Neutrophil Chemotaxis"

Article Title: Giardia duodenalis Cathepsin B Proteases Degrade Intestinal Epithelial Interleukin-8 and Attenuate Interleukin-8-Induced Neutrophil Chemotaxis

Journal: Infection and Immunity

doi: 10.1128/IAI.01771-14

Giardia duodenalis trophozoites secrete cysteine proteases that are inhibited by E-64d and Ca-074Me. Caco-2 monolayers were pretreated with 1 μM E-64d (A) or increasing concentrations of Ca-074Me (1, 10, or 50 μM) (B to E) and subsequently coincubated with G. duodenalis NF trophozoites (MOI, 10:1) for 6 h. Supernatan ts were incubated with the cathepsin B and L fluorogenic substrate ZFR-AMC (A and B) or the catB fluorogenic substrate ZRR-AMC (C) (200 μM, 5 min, 37°C, and pH 7.2). Proteolytic activity was determined by graphing the change in reflective light units over time. Caco-2 monolayers not containing G. duodenalis trophozoites were used as controls. Supernatants were collected, and the log total of supernatant trophozoites (D) and the ratio of motile to nonmotile trophozoites (E) were determined via enumeration on a hemocytometer. (F) Giardia duodenalis NF and GS/M trophozoite sonicates were run through an SDS-PAGE gel copolymerized with Z-Phe-Arg-AMC under nondenaturing conditions and visualized with a ChemiDoc XRS system. All data are representative of at least two independent experiments ( n = 2 or 3/group) and represented as means ± SEM. n.s., not significant. *, P
Figure Legend Snippet: Giardia duodenalis trophozoites secrete cysteine proteases that are inhibited by E-64d and Ca-074Me. Caco-2 monolayers were pretreated with 1 μM E-64d (A) or increasing concentrations of Ca-074Me (1, 10, or 50 μM) (B to E) and subsequently coincubated with G. duodenalis NF trophozoites (MOI, 10:1) for 6 h. Supernatan ts were incubated with the cathepsin B and L fluorogenic substrate ZFR-AMC (A and B) or the catB fluorogenic substrate ZRR-AMC (C) (200 μM, 5 min, 37°C, and pH 7.2). Proteolytic activity was determined by graphing the change in reflective light units over time. Caco-2 monolayers not containing G. duodenalis trophozoites were used as controls. Supernatants were collected, and the log total of supernatant trophozoites (D) and the ratio of motile to nonmotile trophozoites (E) were determined via enumeration on a hemocytometer. (F) Giardia duodenalis NF and GS/M trophozoite sonicates were run through an SDS-PAGE gel copolymerized with Z-Phe-Arg-AMC under nondenaturing conditions and visualized with a ChemiDoc XRS system. All data are representative of at least two independent experiments ( n = 2 or 3/group) and represented as means ± SEM. n.s., not significant. *, P

Techniques Used: Incubation, Activity Assay, SDS Page

14) Product Images from "Neuritin Activates Insulin Receptor Pathway to Up-regulate Kv4.2-mediated Transient Outward K+ Current in Rat Cerebellar Granule Neurons *"

Article Title: Neuritin Activates Insulin Receptor Pathway to Up-regulate Kv4.2-mediated Transient Outward K+ Current in Rat Cerebellar Granule Neurons *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M112.390260

Neuritin-induced I A densities and the increased expression of Kv4.2 α-subunit were dependent the Akt/mTOR signaling pathway. A , Western blot showing the levels of activated Akt (pAkt) and activated mTOR (p-mTOR) in CGNs incubated with 150 ng/ml neuritin for 15 min to 24 h. Chemiluminescent signals were detected with the ChemiDoc XRS System. B , effect of mTOR inhibitor, rapamycin, and Akt inhibitor LY294002 on neuritin-induced up-regulation of I A densities. C and D , effect of rapamycin and LY294002 on neuritin-induced up-regulation of Kv4.2 protein level. E , effect of rapamycin on neuritin-induced up-regulation of Kv4.2 mRNA expression measured by quantitative RT-PCR experiments. Data are shown as means ± S.E. ( error bars ). *, p
Figure Legend Snippet: Neuritin-induced I A densities and the increased expression of Kv4.2 α-subunit were dependent the Akt/mTOR signaling pathway. A , Western blot showing the levels of activated Akt (pAkt) and activated mTOR (p-mTOR) in CGNs incubated with 150 ng/ml neuritin for 15 min to 24 h. Chemiluminescent signals were detected with the ChemiDoc XRS System. B , effect of mTOR inhibitor, rapamycin, and Akt inhibitor LY294002 on neuritin-induced up-regulation of I A densities. C and D , effect of rapamycin and LY294002 on neuritin-induced up-regulation of Kv4.2 protein level. E , effect of rapamycin on neuritin-induced up-regulation of Kv4.2 mRNA expression measured by quantitative RT-PCR experiments. Data are shown as means ± S.E. ( error bars ). *, p

Techniques Used: Expressing, Western Blot, Incubation, Quantitative RT-PCR

Neuritin increased the expression levels of Kv4.2 α-subunit rather than Kv4.3 or Kv1.1 in CGNs. A , Western blot showing effect of 150 ng/ml neuritin on different Kv channels expression levels in CGNs with different DIC. Left , representative Western blots. Right , statistical analysis. B , Western blot results of Kv4.2 α-subunit protein expression levels in CGNs, showing the effect of incubating with 150 ng/ml neuritin for 12–36 h. Chemiluminescent signals were detected using the ChemiDoc XRS System. *, p
Figure Legend Snippet: Neuritin increased the expression levels of Kv4.2 α-subunit rather than Kv4.3 or Kv1.1 in CGNs. A , Western blot showing effect of 150 ng/ml neuritin on different Kv channels expression levels in CGNs with different DIC. Left , representative Western blots. Right , statistical analysis. B , Western blot results of Kv4.2 α-subunit protein expression levels in CGNs, showing the effect of incubating with 150 ng/ml neuritin for 12–36 h. Chemiluminescent signals were detected using the ChemiDoc XRS System. *, p

Techniques Used: Expressing, Western Blot

15) Product Images from "Reprogramming of Yersinia from Virulent to Persistent Mode Revealed by Complex In Vivo RNA-seq Analysis"

Article Title: Reprogramming of Yersinia from Virulent to Persistent Mode Revealed by Complex In Vivo RNA-seq Analysis

Journal: PLoS Pathogens

doi: 10.1371/journal.ppat.1004600

Establishment of persistent infection requires arcA, fnr, frdA , and wrbA . (A) Infection profile 42 dpi for FVB/N mice infected orally with10 7 CFUs of wt Y. pseudotuberculosis (n = 20) and indicated mutant strains (each group n = 16). The infections were monitored by IVIS at certain intervals up to 42 dpi. (B) Heatmap showing differences in clearance (by p -value) between wt and indicated mutant strains at different time points during the 42 day infection period. Heatmap color scale, from green to yellow, was adjusted according to p -values from 1 to 0. p -values were calculated with 2×2 contingency table by Fisher’s Exact Test, see also S6 Table . (C) Motility profile of wt Y. pseudotuberculosis and indicated mutant strains under anaerobic conditions at 26°C. Images were captured by the ChemiDoc XRS System (Bio-Rad), showing the bioluminescent signal produced by Y. pseudotuberculosis YPIII/pIBX.
Figure Legend Snippet: Establishment of persistent infection requires arcA, fnr, frdA , and wrbA . (A) Infection profile 42 dpi for FVB/N mice infected orally with10 7 CFUs of wt Y. pseudotuberculosis (n = 20) and indicated mutant strains (each group n = 16). The infections were monitored by IVIS at certain intervals up to 42 dpi. (B) Heatmap showing differences in clearance (by p -value) between wt and indicated mutant strains at different time points during the 42 day infection period. Heatmap color scale, from green to yellow, was adjusted according to p -values from 1 to 0. p -values were calculated with 2×2 contingency table by Fisher’s Exact Test, see also S6 Table . (C) Motility profile of wt Y. pseudotuberculosis and indicated mutant strains under anaerobic conditions at 26°C. Images were captured by the ChemiDoc XRS System (Bio-Rad), showing the bioluminescent signal produced by Y. pseudotuberculosis YPIII/pIBX.

Techniques Used: Infection, Mouse Assay, Mutagenesis, Produced

16) Product Images from "A novel bi-domain plant defensin MtDef5 with potent broad-spectrum antifungal activity binds to multiple phospholipids and forms oligomers"

Article Title: A novel bi-domain plant defensin MtDef5 with potent broad-spectrum antifungal activity binds to multiple phospholipids and forms oligomers

Journal: Scientific Reports

doi: 10.1038/s41598-017-16508-w

MtDef5 forms oligomers only in presence of PIP. ( A ) MtDef5 forms higher order oligomers in presence of PI3P, PI4P and PI5P as revealed by a protein cross-linking with BS 3 followed by SDS-PAGE and visualized using a Bio-Rad ChemiDoc XRS + system. ( B ) MtDef5 also forms oligomers in presence of PA and PI. The full length gel is presented in Supplementary Figure S5 .
Figure Legend Snippet: MtDef5 forms oligomers only in presence of PIP. ( A ) MtDef5 forms higher order oligomers in presence of PI3P, PI4P and PI5P as revealed by a protein cross-linking with BS 3 followed by SDS-PAGE and visualized using a Bio-Rad ChemiDoc XRS + system. ( B ) MtDef5 also forms oligomers in presence of PA and PI. The full length gel is presented in Supplementary Figure S5 .

Techniques Used: SDS Page

Related Articles

Centrifugation:

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Article Snippet: The lysates were then clarified by centrifugation and normalised based on total protein concentration using the Pierce BCA Protein Assay kit (Thermo Fisher Scientific). .. ProQ diamond and Sypro ruby staining (Thermo Fisher Scientific ) of gels was visualised and quantified on a ChemiDoc imaging system (Bio-Rad).

DNA Synthesis:

Article Title: PCNA Damage Caused by Anti-Neoplastic Drugs
Article Snippet: The experiment was repeated, using a BioRad ChemiDoc™ XRS imaging system to quantitate the area of each lane corresponding to the 93 kDa band (not shown), and confirmed that m -AMSA and light exposure did not produce a detectable high molecular weight PCNA band. .. To test the possibility that these drugs might reach PCNA as a result of nuclear membrane breakdown during mitosis, the experiment was also done in SV40 infected CV-1 cells that are locked into DNA synthesis and unable to progress to mitosis [ – ].

Mass Spectrometry:

Article Title: Murine leukemia virus p12 tethers the capsid-containing pre-integration complex to chromatin by binding directly to host nucleosomes in mitosis
Article Snippet: ProQ diamond and Sypro ruby staining (Thermo Fisher Scientific ) of gels was visualised and quantified on a ChemiDoc imaging system (Bio-Rad). .. This analysis was performed on an LTQ-Orbitrap Velos mass spectrometer in line with a Dionex Ultimate 3000 nanoHPLC system as described in [ ].

Blocking Assay:

Article Title: Distribution of insulin in trigeminal nerve and brain after intranasal administration
Article Snippet: Membranes were incubated overnight at 4 °C in blocking buffer with rabbit anti-phospho(Y1185)-insulin receptor (1:1000). .. Bands were visualized using ECL on a Bio-Rad ChemiDoc imaging system.

Article Title: Association of cellular and molecular responses in the rat mammary gland to 17?-estradiol with susceptibility to mammary cancer
Article Snippet: The blots were incubated with Odyssey blocking buffer (LI-COR) for 1 h at room temperature; incubated overnight at 4°C with primary antibody diluted in Odyssey blocking buffer containing 0.1% Tween-20 as described in Additional file : Table S1; washed four times for 5 minutes each with 0.1% PBST; incubated with the appropriate IRDye-conjugated secondary antibody (Additional file : Table S1) for 1 h at room temperature in the dark; washed four times for 5 minutes each with 0.1% PBST; imaged and quantified. .. Cleaved caspase 3, Mmp7 and Mmp9 were quantified using the ChemiDoc XRS + imaging system (Bio-Rad).

Article Title: Morphine decreases the pro-angiogenic interaction between breast cancer cells and macrophages in vitro
Article Snippet: Mouse angiogenesis antibody array Membranes were placed in a 2-well plate in blocking buffer for 30 min at room temperature according to the manufacturer’s protocol. .. The images were captured using a ChemiDoc Touch Imaging System (Bio-Rad Laboratories Inc.).

Incubation:

Article Title: Distribution of insulin in trigeminal nerve and brain after intranasal administration
Article Snippet: Membranes were washed and incubated in HRP-conjugated anti-rabbit IgG secondary antibody (1:2000) for 1 h at room temperature. .. Bands were visualized using ECL on a Bio-Rad ChemiDoc imaging system.

Article Title: Association of cellular and molecular responses in the rat mammary gland to 17?-estradiol with susceptibility to mammary cancer
Article Snippet: The blots were incubated with Odyssey blocking buffer (LI-COR) for 1 h at room temperature; incubated overnight at 4°C with primary antibody diluted in Odyssey blocking buffer containing 0.1% Tween-20 as described in Additional file : Table S1; washed four times for 5 minutes each with 0.1% PBST; incubated with the appropriate IRDye-conjugated secondary antibody (Additional file : Table S1) for 1 h at room temperature in the dark; washed four times for 5 minutes each with 0.1% PBST; imaged and quantified. .. Cleaved caspase 3, Mmp7 and Mmp9 were quantified using the ChemiDoc XRS + imaging system (Bio-Rad).

Article Title: Improvement of Pharmacokinetic Profile of TRAIL via Trimer-Tag Enhances its Antitumor Activity in vivo
Article Snippet: After several washes in TBST, membranes were incubated for 1 hour at room temperature with HRP conjugated Trueblot anti-goat IgG (1:2000 dilution) (cat. 18-8814-33, Rockland, Pottstown, PA, USA). .. The images were acquired using the ChemiDoc Touch Imaging System (Bio-Rad) and the quantification of the amount of TRAIL proteins present in each sample was done using Image Lab software (Bio-Rad).

Article Title: PCNA Damage Caused by Anti-Neoplastic Drugs
Article Snippet: Blocked membranes were incubated with primary antibody overnight at 4 °C, rinsed, then incubated with horseradish peroxidase-conjugated secondary antibody for 1 hr at room temperature. .. Protein was detected by SuperSignal West Pico Chemiluminescence Substrate (Pierce, Rockford, IL) using either X-ray film or the BioRad ChemiDoc™ XRS imaging system.

Article Title: Murine leukemia virus p12 tethers the capsid-containing pre-integration complex to chromatin by binding directly to host nucleosomes in mitosis
Article Snippet: 0.5 ml aliquots of lysates at 1.5–3 mg/ml were incubated with glutathione-sepharose beads (100 μl/reaction of a 50% slurry) (GE Healthcare) for 3 h at 4°C with end-over-end rotation. .. ProQ diamond and Sypro ruby staining (Thermo Fisher Scientific ) of gels was visualised and quantified on a ChemiDoc imaging system (Bio-Rad).

Article Title: A surface-exposed neuraminidase affects complement resistance and virulence of the oral spirochete Treponema denticola
Article Snippet: Briefly, the recombinant TDE0471, the whole cell lysates, or the supernatants from the T. denticola cultures were incubated with 2’-4-methylumbelliferyl-α-D- N -acetyl-neuraminic acid (4-MUNANA) (Sigma-Aldrich), a fluorogenic neuraminidase substrate. .. Fluorescence was measured using a ChemiDoc XRS imaging system (Bio-Rad) with an excitation wavelength of 302 nm and an emission wavelength of 548 nm.

Article Title: Proteolytic inactivation of nuclear alarmin high-mobility group box 1 by complement protease C1s during apoptosis
Article Snippet: After incubation for 1 h at 37 °C, the reactions were subjected to SDS-PAGE on 12.5% (w/v) gels and analyzed by western blotting with overnight incubation with rheumatological patients’ sera (1 : 5000 dilutions) and then horseradish peroxidase-conjugated goat anti-human IgG Fc (1 : 20 000 dilutions; Pierce, Rockford, IL, USA). .. Signals were visualized using the MPS ChemiDoc imaging system (Bio-Rad Lab., Hercules, CA, USA).

Article Title: PCNA Damage Caused by Anti-Neoplastic Drugs
Article Snippet: None of these drugs caused high molecular weight PCNA forms when incubated with the cells in the dark ( ). m -AMSA, chloroquine and noscapine did not produce detectable high molecular weight PCNA bands with exposure to light. .. The experiment was repeated, using a BioRad ChemiDoc™ XRS imaging system to quantitate the area of each lane corresponding to the 93 kDa band (not shown), and confirmed that m -AMSA and light exposure did not produce a detectable high molecular weight PCNA band.

Activity Assay:

Article Title: A surface-exposed neuraminidase affects complement resistance and virulence of the oral spirochete Treponema denticola
Article Snippet: A previously documented filter paper spot sialidase assay was used to detect the neuraminidase activity ( ; ). .. Fluorescence was measured using a ChemiDoc XRS imaging system (Bio-Rad) with an excitation wavelength of 302 nm and an emission wavelength of 548 nm.

Expressing:

Article Title: Murine leukemia virus p12 tethers the capsid-containing pre-integration complex to chromatin by binding directly to host nucleosomes in mitosis
Article Snippet: ProQ diamond and Sypro ruby staining (Thermo Fisher Scientific ) of gels was visualised and quantified on a ChemiDoc imaging system (Bio-Rad). .. For SILAC-MS experiments, 293T cells were grown is media containing light (R0K0, 12 C-Arginine and 12 C-Lysine), medium (R6K4, 13 C-Arginine and Lysine-4,4,5,5-d4) or heavy (R10K8, 13 C+15 N-Arginine and 13 C+15 N-Lysine) versions of L-Arginine and L-Lysine amino acids (Pierce) for a minimum of 6 doublings before transient expression of GST-p12 proteins.

BIA-KA:

Article Title: Immune-enhancing effects of anionic macromolecules extracted from Codium fragile on cyclophosphamide-treated mice
Article Snippet: Protein concentration was measured using the Pierce™ BCA Protein Assay kit (Thermo Scientific, USA). .. Imaging was performed using the ChemiDoc XRS+ imaging system (Bio-Rad) and protein band intensities were analyzed using the ImageLab software (version 4.1, Bio-Rad).

Article Title: Distribution of insulin in trigeminal nerve and brain after intranasal administration
Article Snippet: Equal amounts of protein (determined by BCA protein assay) from brain homogenates of rats treated with IN saline or IN insulin were loaded onto Any KD TGX gels and subjected to SDS-PAGE. .. Bands were visualized using ECL on a Bio-Rad ChemiDoc imaging system.

Article Title: Association of cellular and molecular responses in the rat mammary gland to 17?-estradiol with susceptibility to mammary cancer
Article Snippet: The lysates were centrifuged at 12,000g for 30 min, the supernatants were collected and protein concentration was determined using BCA Protein Assay Reagent (Thermo Fisher). .. Cleaved caspase 3, Mmp7 and Mmp9 were quantified using the ChemiDoc XRS + imaging system (Bio-Rad).

Article Title: Murine leukemia virus p12 tethers the capsid-containing pre-integration complex to chromatin by binding directly to host nucleosomes in mitosis
Article Snippet: The lysates were then clarified by centrifugation and normalised based on total protein concentration using the Pierce BCA Protein Assay kit (Thermo Fisher Scientific). .. ProQ diamond and Sypro ruby staining (Thermo Fisher Scientific ) of gels was visualised and quantified on a ChemiDoc imaging system (Bio-Rad).

Western Blot:

Article Title: Immune-enhancing effects of anionic macromolecules extracted from Codium fragile on cyclophosphamide-treated mice
Article Snippet: Paragraph title: Western blotting for analyzing the NF-κB and MAPK signaling pathways ... Imaging was performed using the ChemiDoc XRS+ imaging system (Bio-Rad) and protein band intensities were analyzed using the ImageLab software (version 4.1, Bio-Rad).

Article Title: Distribution of insulin in trigeminal nerve and brain after intranasal administration
Article Snippet: Paragraph title: Western blot analysis ... Bands were visualized using ECL on a Bio-Rad ChemiDoc imaging system.

Article Title: Association of cellular and molecular responses in the rat mammary gland to 17?-estradiol with susceptibility to mammary cancer
Article Snippet: Paragraph title: Quantitative western blotting ... Cleaved caspase 3, Mmp7 and Mmp9 were quantified using the ChemiDoc XRS + imaging system (Bio-Rad).

Article Title: Production of human vitronectin in Nicotiana benthamiana using the INPACT hyperexpression platform
Article Snippet: For yield estimation, Clarity Western ECL substrate (Bio‐Rad) was formulated according to the manufacturer and applied as a 1 : 10 dilution to the membrane. .. Signal strength was detected using a ChemiDoc imaging system (Bio‐Rad) and yield calculated from a hVN standard curve ranging from 50 to 500 ng.

Article Title: PCNA Damage Caused by Anti-Neoplastic Drugs
Article Snippet: Paragraph title: 2.4. Western blotting and immunoprecipitation ... Protein was detected by SuperSignal West Pico Chemiluminescence Substrate (Pierce, Rockford, IL) using either X-ray film or the BioRad ChemiDoc™ XRS imaging system.

Article Title: Proteolytic inactivation of nuclear alarmin high-mobility group box 1 by complement protease C1s during apoptosis
Article Snippet: After incubation for 1 h at 37 °C, the reactions were subjected to SDS-PAGE on 12.5% (w/v) gels and analyzed by western blotting with overnight incubation with rheumatological patients’ sera (1 : 5000 dilutions) and then horseradish peroxidase-conjugated goat anti-human IgG Fc (1 : 20 000 dilutions; Pierce, Rockford, IL, USA). .. Signals were visualized using the MPS ChemiDoc imaging system (Bio-Rad Lab., Hercules, CA, USA).

Immunoprecipitation:

Article Title: PCNA Damage Caused by Anti-Neoplastic Drugs
Article Snippet: Paragraph title: 2.4. Western blotting and immunoprecipitation ... Protein was detected by SuperSignal West Pico Chemiluminescence Substrate (Pierce, Rockford, IL) using either X-ray film or the BioRad ChemiDoc™ XRS imaging system.

Protease Inhibitor:

Article Title: PCNA Damage Caused by Anti-Neoplastic Drugs
Article Snippet: Whole cell protein extracts were prepared in SDS lysis buffer (62.5 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 1 mM PMSF, 10 µg/ml of each protease inhibitor, aprotinin, leupeptin, and pepstatin. .. Protein was detected by SuperSignal West Pico Chemiluminescence Substrate (Pierce, Rockford, IL) using either X-ray film or the BioRad ChemiDoc™ XRS imaging system.

Infection:

Article Title: PCNA Damage Caused by Anti-Neoplastic Drugs
Article Snippet: The experiment was repeated, using a BioRad ChemiDoc™ XRS imaging system to quantitate the area of each lane corresponding to the 93 kDa band (not shown), and confirmed that m -AMSA and light exposure did not produce a detectable high molecular weight PCNA band. .. To test the possibility that these drugs might reach PCNA as a result of nuclear membrane breakdown during mitosis, the experiment was also done in SV40 infected CV-1 cells that are locked into DNA synthesis and unable to progress to mitosis [ – ].

SDS Page:

Article Title: Immune-enhancing effects of anionic macromolecules extracted from Codium fragile on cyclophosphamide-treated mice
Article Snippet: After resolving the proteins using SDS-polyacrylamide gel electrophoresis (SDS-PAGE), they were transferred on to a polyvinylidene fluoride (PVDF) membrane. .. Imaging was performed using the ChemiDoc XRS+ imaging system (Bio-Rad) and protein band intensities were analyzed using the ImageLab software (version 4.1, Bio-Rad).

Article Title: Distribution of insulin in trigeminal nerve and brain after intranasal administration
Article Snippet: Equal amounts of protein (determined by BCA protein assay) from brain homogenates of rats treated with IN saline or IN insulin were loaded onto Any KD TGX gels and subjected to SDS-PAGE. .. Bands were visualized using ECL on a Bio-Rad ChemiDoc imaging system.

Article Title: Association of cellular and molecular responses in the rat mammary gland to 17?-estradiol with susceptibility to mammary cancer
Article Snippet: Briefly, mammary proteins were separated by SDS-PAGE and transferred to Immobilon-FL PVDF membranes (Millipore, Billerica, MA). .. Cleaved caspase 3, Mmp7 and Mmp9 were quantified using the ChemiDoc XRS + imaging system (Bio-Rad).

Article Title: Production of human vitronectin in Nicotiana benthamiana using the INPACT hyperexpression platform
Article Snippet: For amino‐terminal sequencing, 3 μg of plant‐made hVN was subjected to SDS‐PAGE, transferred to PVDF membrane and stained with Ponceau dye. .. Signal strength was detected using a ChemiDoc imaging system (Bio‐Rad) and yield calculated from a hVN standard curve ranging from 50 to 500 ng.

Article Title: PCNA Damage Caused by Anti-Neoplastic Drugs
Article Snippet: Proteins (20–40 µg) were separated by SDS-PAGE (polyacrylamide gel electrophoresis) and transferred to a nitrocellulose membrane (Schleicher & Schuell, Perkin Elmer, Shelton, CT) using a BioRad (Hercules, CA) semi-dry transfer system. .. Protein was detected by SuperSignal West Pico Chemiluminescence Substrate (Pierce, Rockford, IL) using either X-ray film or the BioRad ChemiDoc™ XRS imaging system.

Article Title: Murine leukemia virus p12 tethers the capsid-containing pre-integration complex to chromatin by binding directly to host nucleosomes in mitosis
Article Snippet: The beads were then washed three times in 20 mM Tris pH 8.0, 300 mM NaCl, 1 mM EDTA, 10% Glycerol, 0.1% Triton X-100, protease inhibitors and re-suspended in 50 μl of x2 protein loading dye for SDS-PAGE and immunoblotting. .. ProQ diamond and Sypro ruby staining (Thermo Fisher Scientific ) of gels was visualised and quantified on a ChemiDoc imaging system (Bio-Rad).

Article Title: Proteolytic inactivation of nuclear alarmin high-mobility group box 1 by complement protease C1s during apoptosis
Article Snippet: After incubation for 1 h at 37 °C, the reactions were subjected to SDS-PAGE on 12.5% (w/v) gels and analyzed by western blotting with overnight incubation with rheumatological patients’ sera (1 : 5000 dilutions) and then horseradish peroxidase-conjugated goat anti-human IgG Fc (1 : 20 000 dilutions; Pierce, Rockford, IL, USA). .. Signals were visualized using the MPS ChemiDoc imaging system (Bio-Rad Lab., Hercules, CA, USA).

Imaging:

Article Title: Immune-enhancing effects of anionic macromolecules extracted from Codium fragile on cyclophosphamide-treated mice
Article Snippet: .. Imaging was performed using the ChemiDoc XRS+ imaging system (Bio-Rad) and protein band intensities were analyzed using the ImageLab software (version 4.1, Bio-Rad). .. Effect of NF-κB and MAPK activation inhibitors on TNF-α expression In order to evaluate the effects of NF-κB and MAPK activation inhibitors (such as ERK, JNK, and p38) on TNF-α expression, RAW264.7 cells were used.

Article Title: Distribution of insulin in trigeminal nerve and brain after intranasal administration
Article Snippet: .. Bands were visualized using ECL on a Bio-Rad ChemiDoc imaging system. .. Because insulin has previously been shown to alter common housekeeping genes used as loading controls such as actin, tubulin, and GAPDH, the optical density of the bands was quantified using FIJI and normalized to a total protein stain according to previously published methods – .

Article Title: Association of cellular and molecular responses in the rat mammary gland to 17?-estradiol with susceptibility to mammary cancer
Article Snippet: .. Cleaved caspase 3, Mmp7 and Mmp9 were quantified using the ChemiDoc XRS + imaging system (Bio-Rad). ..

Article Title: Production of human vitronectin in Nicotiana benthamiana using the INPACT hyperexpression platform
Article Snippet: .. Signal strength was detected using a ChemiDoc imaging system (Bio‐Rad) and yield calculated from a hVN standard curve ranging from 50 to 500 ng. ..

Article Title: Improvement of Pharmacokinetic Profile of TRAIL via Trimer-Tag Enhances its Antitumor Activity in vivo
Article Snippet: .. The images were acquired using the ChemiDoc Touch Imaging System (Bio-Rad) and the quantification of the amount of TRAIL proteins present in each sample was done using Image Lab software (Bio-Rad). .. COLO205 tumor xenograft experiments in nude mice Female nude mice were purchased from Beijing HFK Bioscience Co, Ltd. (Beijing, China) and kept under standard pathogen-free conditions in the animal care center at Sichuan University and received human care.

Article Title: PCNA Damage Caused by Anti-Neoplastic Drugs
Article Snippet: .. Protein was detected by SuperSignal West Pico Chemiluminescence Substrate (Pierce, Rockford, IL) using either X-ray film or the BioRad ChemiDoc™ XRS imaging system. .. Primary antibodies were mouse PC10 or goat C20 (Santa Cruz Biotech, Santa Cruz, CA) directed against PCNA, mouse anti-HA tag (Roche, Indianapolis, IN), mouse anti-SV40 large T antigen (Lab Vision, Fremont, CA), goat anti-lamin B (Santa Cruz), goat anti-triose phosphate isomerase (Novus, Littleton, CO), and mouse monoclonal anti-ubiquitin (Biomol, Exeter, UK).

Article Title: Murine leukemia virus p12 tethers the capsid-containing pre-integration complex to chromatin by binding directly to host nucleosomes in mitosis
Article Snippet: .. ProQ diamond and Sypro ruby staining (Thermo Fisher Scientific ) of gels was visualised and quantified on a ChemiDoc imaging system (Bio-Rad). .. For SILAC-MS experiments, 293T cells were grown is media containing light (R0K0, 12 C-Arginine and 12 C-Lysine), medium (R6K4, 13 C-Arginine and Lysine-4,4,5,5-d4) or heavy (R10K8, 13 C+15 N-Arginine and 13 C+15 N-Lysine) versions of L-Arginine and L-Lysine amino acids (Pierce) for a minimum of 6 doublings before transient expression of GST-p12 proteins.

Article Title: A surface-exposed neuraminidase affects complement resistance and virulence of the oral spirochete Treponema denticola
Article Snippet: .. Fluorescence was measured using a ChemiDoc XRS imaging system (Bio-Rad) with an excitation wavelength of 302 nm and an emission wavelength of 548 nm. ..

Article Title: Secretory phospholipase A2 modified HDL rapidly and potently suppresses platelet activation
Article Snippet: .. The membranes were imaged using ChemiDoc Touch Imaging System and ECL Blotting Substrate (both Bio-Rad, Vienna, Austria). .. The average signal (pixel density) analysis of duplicate spots representing each protein was performed using Image Lab 5.2 software (Bio-Rad).

Article Title: Proteolytic inactivation of nuclear alarmin high-mobility group box 1 by complement protease C1s during apoptosis
Article Snippet: .. Signals were visualized using the MPS ChemiDoc imaging system (Bio-Rad Lab., Hercules, CA, USA). .. SDS-PAGE and western blotting Samples were reduced by heating in the presence of dithiothreitol (10 mM) and separated on 12.5% (w/v) gels.

Article Title: Morphine decreases the pro-angiogenic interaction between breast cancer cells and macrophages in vitro
Article Snippet: .. The images were captured using a ChemiDoc Touch Imaging System (Bio-Rad Laboratories Inc.). ..

Article Title: PCNA Damage Caused by Anti-Neoplastic Drugs
Article Snippet: .. The experiment was repeated, using a BioRad ChemiDoc™ XRS imaging system to quantitate the area of each lane corresponding to the 93 kDa band (not shown), and confirmed that m -AMSA and light exposure did not produce a detectable high molecular weight PCNA band. .. Hypericin, an endoplasmic reticulum localizing drug [ ], was a very effective photodynamic producer of the 93 and 154 kDa bands, as well as the minor high molecular weight PCNA bands produced by proflavine ( ).

Protein Concentration:

Article Title: Immune-enhancing effects of anionic macromolecules extracted from Codium fragile on cyclophosphamide-treated mice
Article Snippet: Protein concentration was measured using the Pierce™ BCA Protein Assay kit (Thermo Scientific, USA). .. Imaging was performed using the ChemiDoc XRS+ imaging system (Bio-Rad) and protein band intensities were analyzed using the ImageLab software (version 4.1, Bio-Rad).

Article Title: Association of cellular and molecular responses in the rat mammary gland to 17?-estradiol with susceptibility to mammary cancer
Article Snippet: The lysates were centrifuged at 12,000g for 30 min, the supernatants were collected and protein concentration was determined using BCA Protein Assay Reagent (Thermo Fisher). .. Cleaved caspase 3, Mmp7 and Mmp9 were quantified using the ChemiDoc XRS + imaging system (Bio-Rad).

Article Title: Murine leukemia virus p12 tethers the capsid-containing pre-integration complex to chromatin by binding directly to host nucleosomes in mitosis
Article Snippet: The lysates were then clarified by centrifugation and normalised based on total protein concentration using the Pierce BCA Protein Assay kit (Thermo Fisher Scientific). .. ProQ diamond and Sypro ruby staining (Thermo Fisher Scientific ) of gels was visualised and quantified on a ChemiDoc imaging system (Bio-Rad).

Sequencing:

Article Title: Production of human vitronectin in Nicotiana benthamiana using the INPACT hyperexpression platform
Article Snippet: Paragraph title: PAGE, immunoblotting, N‐terminus sequencing and yield estimation ... Signal strength was detected using a ChemiDoc imaging system (Bio‐Rad) and yield calculated from a hVN standard curve ranging from 50 to 500 ng.

Sonication:

Article Title: Murine leukemia virus p12 tethers the capsid-containing pre-integration complex to chromatin by binding directly to host nucleosomes in mitosis
Article Snippet: After sonication, the lysates were supplemented with 4 mM MgCl2 and Pierce universal nuclease (Thermo Fisher Scientific) and incubated for a further 1 h at 4°C. .. ProQ diamond and Sypro ruby staining (Thermo Fisher Scientific ) of gels was visualised and quantified on a ChemiDoc imaging system (Bio-Rad).

Recombinant:

Article Title: A surface-exposed neuraminidase affects complement resistance and virulence of the oral spirochete Treponema denticola
Article Snippet: Briefly, the recombinant TDE0471, the whole cell lysates, or the supernatants from the T. denticola cultures were incubated with 2’-4-methylumbelliferyl-α-D- N -acetyl-neuraminic acid (4-MUNANA) (Sigma-Aldrich), a fluorogenic neuraminidase substrate. .. Fluorescence was measured using a ChemiDoc XRS imaging system (Bio-Rad) with an excitation wavelength of 302 nm and an emission wavelength of 548 nm.

Molecular Weight:

Article Title: PCNA Damage Caused by Anti-Neoplastic Drugs
Article Snippet: Protein was detected by SuperSignal West Pico Chemiluminescence Substrate (Pierce, Rockford, IL) using either X-ray film or the BioRad ChemiDoc™ XRS imaging system. .. The apparent molecular weight of each PCNA form induced by photodynamic damage was estimated by comparison to commercial marker sets: Precision Plus Protein™ Dual color standards (BioRad) and EZ-Run™ pre-stained Rec Protein ladder (Fisher Scientific, Pittsburgh, PA).

Article Title: PCNA Damage Caused by Anti-Neoplastic Drugs
Article Snippet: .. The experiment was repeated, using a BioRad ChemiDoc™ XRS imaging system to quantitate the area of each lane corresponding to the 93 kDa band (not shown), and confirmed that m -AMSA and light exposure did not produce a detectable high molecular weight PCNA band. .. Hypericin, an endoplasmic reticulum localizing drug [ ], was a very effective photodynamic producer of the 93 and 154 kDa bands, as well as the minor high molecular weight PCNA bands produced by proflavine ( ).

Nucleic Acid Electrophoresis:

Article Title: Immune-enhancing effects of anionic macromolecules extracted from Codium fragile on cyclophosphamide-treated mice
Article Snippet: After resolving the proteins using SDS-polyacrylamide gel electrophoresis (SDS-PAGE), they were transferred on to a polyvinylidene fluoride (PVDF) membrane. .. Imaging was performed using the ChemiDoc XRS+ imaging system (Bio-Rad) and protein band intensities were analyzed using the ImageLab software (version 4.1, Bio-Rad).

Fluorescence:

Article Title: A surface-exposed neuraminidase affects complement resistance and virulence of the oral spirochete Treponema denticola
Article Snippet: .. Fluorescence was measured using a ChemiDoc XRS imaging system (Bio-Rad) with an excitation wavelength of 302 nm and an emission wavelength of 548 nm. ..

Mouse Assay:

Article Title: Improvement of Pharmacokinetic Profile of TRAIL via Trimer-Tag Enhances its Antitumor Activity in vivo
Article Snippet: Paragraph title: Pharmacokinetics study and analysis in nude mice ... The images were acquired using the ChemiDoc Touch Imaging System (Bio-Rad) and the quantification of the amount of TRAIL proteins present in each sample was done using Image Lab software (Bio-Rad).

Co-Culture Assay:

Article Title: Morphine decreases the pro-angiogenic interaction between breast cancer cells and macrophages in vitro
Article Snippet: Co-culture conditioned medium used in each experiment was a mix of three separate experiments, and the array assay was performed three times (so that a total of 9 separate co-culture conditioned media were employed). .. The images were captured using a ChemiDoc Touch Imaging System (Bio-Rad Laboratories Inc.).

Polyacrylamide Gel Electrophoresis:

Article Title: Production of human vitronectin in Nicotiana benthamiana using the INPACT hyperexpression platform
Article Snippet: Paragraph title: PAGE, immunoblotting, N‐terminus sequencing and yield estimation ... Signal strength was detected using a ChemiDoc imaging system (Bio‐Rad) and yield calculated from a hVN standard curve ranging from 50 to 500 ng.

Article Title: PCNA Damage Caused by Anti-Neoplastic Drugs
Article Snippet: Proteins (20–40 µg) were separated by SDS-PAGE (polyacrylamide gel electrophoresis) and transferred to a nitrocellulose membrane (Schleicher & Schuell, Perkin Elmer, Shelton, CT) using a BioRad (Hercules, CA) semi-dry transfer system. .. Protein was detected by SuperSignal West Pico Chemiluminescence Substrate (Pierce, Rockford, IL) using either X-ray film or the BioRad ChemiDoc™ XRS imaging system.

Staining:

Article Title: Distribution of insulin in trigeminal nerve and brain after intranasal administration
Article Snippet: Bands were visualized using ECL on a Bio-Rad ChemiDoc imaging system. .. Because insulin has previously been shown to alter common housekeeping genes used as loading controls such as actin, tubulin, and GAPDH, the optical density of the bands was quantified using FIJI and normalized to a total protein stain according to previously published methods – .

Article Title: Production of human vitronectin in Nicotiana benthamiana using the INPACT hyperexpression platform
Article Snippet: For amino‐terminal sequencing, 3 μg of plant‐made hVN was subjected to SDS‐PAGE, transferred to PVDF membrane and stained with Ponceau dye. .. Signal strength was detected using a ChemiDoc imaging system (Bio‐Rad) and yield calculated from a hVN standard curve ranging from 50 to 500 ng.

Article Title: Murine leukemia virus p12 tethers the capsid-containing pre-integration complex to chromatin by binding directly to host nucleosomes in mitosis
Article Snippet: .. ProQ diamond and Sypro ruby staining (Thermo Fisher Scientific ) of gels was visualised and quantified on a ChemiDoc imaging system (Bio-Rad). .. For SILAC-MS experiments, 293T cells were grown is media containing light (R0K0, 12 C-Arginine and 12 C-Lysine), medium (R6K4, 13 C-Arginine and Lysine-4,4,5,5-d4) or heavy (R10K8, 13 C+15 N-Arginine and 13 C+15 N-Lysine) versions of L-Arginine and L-Lysine amino acids (Pierce) for a minimum of 6 doublings before transient expression of GST-p12 proteins.

Silver Staining:

Article Title: Murine leukemia virus p12 tethers the capsid-containing pre-integration complex to chromatin by binding directly to host nucleosomes in mitosis
Article Snippet: Silver-staining was performed using the SilverQuest kit (Thermo Fisher Scientific). .. ProQ diamond and Sypro ruby staining (Thermo Fisher Scientific ) of gels was visualised and quantified on a ChemiDoc imaging system (Bio-Rad).

Spot Test:

Article Title: A surface-exposed neuraminidase affects complement resistance and virulence of the oral spirochete Treponema denticola
Article Snippet: Fluorescence was measured using a ChemiDoc XRS imaging system (Bio-Rad) with an excitation wavelength of 302 nm and an emission wavelength of 548 nm. .. To determine the optimal pH for the enzymatic activity, the filter paper spot assay was carried out at various pH values (ranging from 3.0 to 9.0).

Software:

Article Title: Immune-enhancing effects of anionic macromolecules extracted from Codium fragile on cyclophosphamide-treated mice
Article Snippet: .. Imaging was performed using the ChemiDoc XRS+ imaging system (Bio-Rad) and protein band intensities were analyzed using the ImageLab software (version 4.1, Bio-Rad). .. Effect of NF-κB and MAPK activation inhibitors on TNF-α expression In order to evaluate the effects of NF-κB and MAPK activation inhibitors (such as ERK, JNK, and p38) on TNF-α expression, RAW264.7 cells were used.

Article Title: Association of cellular and molecular responses in the rat mammary gland to 17?-estradiol with susceptibility to mammary cancer
Article Snippet: Cleaved caspase 3, Mmp7 and Mmp9 were quantified using the ChemiDoc XRS + imaging system (Bio-Rad). .. The proteins were visualized using SuperSignal West Dura Chemiluminescent Substrate (Thermo Fisher), imaged and quantified using Image Lab 4.0.1 software (Bio-Rad).

Article Title: Improvement of Pharmacokinetic Profile of TRAIL via Trimer-Tag Enhances its Antitumor Activity in vivo
Article Snippet: .. The images were acquired using the ChemiDoc Touch Imaging System (Bio-Rad) and the quantification of the amount of TRAIL proteins present in each sample was done using Image Lab software (Bio-Rad). .. COLO205 tumor xenograft experiments in nude mice Female nude mice were purchased from Beijing HFK Bioscience Co, Ltd. (Beijing, China) and kept under standard pathogen-free conditions in the animal care center at Sichuan University and received human care.

Article Title: Secretory phospholipase A2 modified HDL rapidly and potently suppresses platelet activation
Article Snippet: The membranes were imaged using ChemiDoc Touch Imaging System and ECL Blotting Substrate (both Bio-Rad, Vienna, Austria). .. The average signal (pixel density) analysis of duplicate spots representing each protein was performed using Image Lab 5.2 software (Bio-Rad).

Irradiation:

Article Title: PCNA Damage Caused by Anti-Neoplastic Drugs
Article Snippet: Camptothecin, doxorubicin, berberine, nitidine, and ethidium bromide produced weak 93 kDa bands when irradiated with fluorescent light. .. The experiment was repeated, using a BioRad ChemiDoc™ XRS imaging system to quantitate the area of each lane corresponding to the 93 kDa band (not shown), and confirmed that m -AMSA and light exposure did not produce a detectable high molecular weight PCNA band.

Negative Control:

Article Title: PCNA Damage Caused by Anti-Neoplastic Drugs
Article Snippet: Topoisomerase poisons and other drugs were also studied for their ability to photodamage PCNA, with ethidium bromide and proflavine as positive controls and noscapine as a negative control ( ). .. The experiment was repeated, using a BioRad ChemiDoc™ XRS imaging system to quantitate the area of each lane corresponding to the 93 kDa band (not shown), and confirmed that m -AMSA and light exposure did not produce a detectable high molecular weight PCNA band.

Produced:

Article Title: PCNA Damage Caused by Anti-Neoplastic Drugs
Article Snippet: Paragraph title: 3.1. High molecular weight PCNA forms produced by photodynamic damage ... The experiment was repeated, using a BioRad ChemiDoc™ XRS imaging system to quantitate the area of each lane corresponding to the 93 kDa band (not shown), and confirmed that m -AMSA and light exposure did not produce a detectable high molecular weight PCNA band.

Concentration Assay:

Article Title: PCNA Damage Caused by Anti-Neoplastic Drugs
Article Snippet: At the same drug concentration and radiant exposure, proflavine and acridine orange produced similar levels of the 93 kDa PCNA form, but very different levels of the 154 kDa form. .. The experiment was repeated, using a BioRad ChemiDoc™ XRS imaging system to quantitate the area of each lane corresponding to the 93 kDa band (not shown), and confirmed that m -AMSA and light exposure did not produce a detectable high molecular weight PCNA band.

Marker:

Article Title: PCNA Damage Caused by Anti-Neoplastic Drugs
Article Snippet: Protein was detected by SuperSignal West Pico Chemiluminescence Substrate (Pierce, Rockford, IL) using either X-ray film or the BioRad ChemiDoc™ XRS imaging system. .. The apparent molecular weight of each PCNA form induced by photodynamic damage was estimated by comparison to commercial marker sets: Precision Plus Protein™ Dual color standards (BioRad) and EZ-Run™ pre-stained Rec Protein ladder (Fisher Scientific, Pittsburgh, PA).

Lysis:

Article Title: Association of cellular and molecular responses in the rat mammary gland to 17?-estradiol with susceptibility to mammary cancer
Article Snippet: Quantitative western blotting Frozen mammary tissues were homogenized with PowerGen Model 35 Handheld Homogenizer (Thermo Fisher Scientific, Waltham, MA) in lysis buffer containing 25 mM HEPES (pH 7.4), 300 mM NaCl, 1.5 mM MgCl2 , 1 mM EGTA, 0.2 mM Na3 VO4 , 50 mM glycerophosphate, 0.5% Triton X-100 and 1% Halt Proteinase and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific). .. Cleaved caspase 3, Mmp7 and Mmp9 were quantified using the ChemiDoc XRS + imaging system (Bio-Rad).

Article Title: PCNA Damage Caused by Anti-Neoplastic Drugs
Article Snippet: Whole cell protein extracts were prepared in SDS lysis buffer (62.5 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 1 mM PMSF, 10 µg/ml of each protease inhibitor, aprotinin, leupeptin, and pepstatin. .. Protein was detected by SuperSignal West Pico Chemiluminescence Substrate (Pierce, Rockford, IL) using either X-ray film or the BioRad ChemiDoc™ XRS imaging system.

Ab Array:

Article Title: Morphine decreases the pro-angiogenic interaction between breast cancer cells and macrophages in vitro
Article Snippet: Paragraph title: Mouse angiogenesis antibody array ... The images were captured using a ChemiDoc Touch Imaging System (Bio-Rad Laboratories Inc.).

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  • 99
    Bio-Rad chemidoc xrs system
    Selective labeling of sfGFPs that contained site-specifically incorporated NCAAs with strained alkene/alkyne functionalities with (A) FITC-TZ and (B) HZCL. In A and B, the top panels show denaturing SDS-PAGE analysis of proteins stained with Coomassie blue and the bottom panels are from fluorescent imaging of the same gels before Coomassie blue staining. In A, the fluorescent image was captured by a digital camera and displayed was real color of the emitting light. In B, the fluorescent image was captured by a Bio-Rad <t>ChemiDoc</t> <t>XRS</t> system.
    Chemidoc Xrs System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chemidoc xrs system/product/Bio-Rad
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    chemidoc xrs system - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad chemidoc xrs imaging system
    Western blot analysis of placenta homogenates using anti-ALDH3B2 and anti-GAPDH antibodies revealed bands corresponding to the molecular weight of 53 kDa (long isoform of ALDH3B2) and bands corresponding to molecular weight of 37 kDa (GAPDH). Proteins in homogenates were applied onto the polyacrylamide gel. After separation, they were transferred onto the PVDF membrane. Immunodetection of ALDH3B2 protein was performed using anti-ALDH3B2 antibody. After membrane stripping, the immunodetection was repeated with anti-GAPDH antibody. GAPDH detection was used as a loading control, as GAPDH gene is constitutively expressed at high levels in many tissues. The image was taken using <t>ChemiDoc</t> <t>XRS+</t> (Bio-Rad). Additionally, the image of the whole membrane was added into the Supplementary Materials (Supplementary Figure S1) .
    Chemidoc Xrs Imaging System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1097 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chemidoc xrs imaging system/product/Bio-Rad
    Average 99 stars, based on 1097 article reviews
    Price from $9.99 to $1999.99
    chemidoc xrs imaging system - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Selective labeling of sfGFPs that contained site-specifically incorporated NCAAs with strained alkene/alkyne functionalities with (A) FITC-TZ and (B) HZCL. In A and B, the top panels show denaturing SDS-PAGE analysis of proteins stained with Coomassie blue and the bottom panels are from fluorescent imaging of the same gels before Coomassie blue staining. In A, the fluorescent image was captured by a digital camera and displayed was real color of the emitting light. In B, the fluorescent image was captured by a Bio-Rad ChemiDoc XRS system.

    Journal: Bioconjugate Chemistry

    Article Title: Two Rapid Catalyst-Free Click Reactions for In Vivo Protein Labeling of Genetically Encoded Strained Alkene/Alkyne Functionalities

    doi: 10.1021/bc500361d

    Figure Lengend Snippet: Selective labeling of sfGFPs that contained site-specifically incorporated NCAAs with strained alkene/alkyne functionalities with (A) FITC-TZ and (B) HZCL. In A and B, the top panels show denaturing SDS-PAGE analysis of proteins stained with Coomassie blue and the bottom panels are from fluorescent imaging of the same gels before Coomassie blue staining. In A, the fluorescent image was captured by a digital camera and displayed was real color of the emitting light. In B, the fluorescent image was captured by a Bio-Rad ChemiDoc XRS system.

    Article Snippet: The fluorescence was detected in a Bio-Rad ChemiDoc XRS+ system under UV irradiation.

    Techniques: Labeling, SDS Page, Staining, Imaging

    Pseudotyped particle protein density analysis. Quantification of intensities of HA, NA and VSV M protein by Western Blot ( A ). The HA 1 ( B ) and NA ( C ) band intensities were directly detected by Chemidoc XRS+ imager and normalized to corresponding M band intensity to obtain the relative HA and NA expression levels on different VSV pseudotyped particles. Each error bar represents the mean ± SD of three independent experiments. NA protein was also detected except for HA only particles.

    Journal: Scientific Reports

    Article Title: Viral fusion efficacy of specific H3N2 influenza virus reassortant combinations at single-particle level

    doi: 10.1038/srep35537

    Figure Lengend Snippet: Pseudotyped particle protein density analysis. Quantification of intensities of HA, NA and VSV M protein by Western Blot ( A ). The HA 1 ( B ) and NA ( C ) band intensities were directly detected by Chemidoc XRS+ imager and normalized to corresponding M band intensity to obtain the relative HA and NA expression levels on different VSV pseudotyped particles. Each error bar represents the mean ± SD of three independent experiments. NA protein was also detected except for HA only particles.

    Article Snippet: All Western blots were visualized and analyzed using a Chemidoc XRS+ system with Image Lab image capture software (BioRad).

    Techniques: Western Blot, Expressing

    Western blot analysis of extracellular matrix components in wound exudates collected from mice after injection of B . asper venom. Groups of five mice were injected in the gastrocnemius with 50 μg of B . asper venom. After 1, 6 and 24 h of injection mice were sacrificed and samples of exudates were collected, pooled and lyophilized. Afterwards, 100 μg of protein of each sample were separated under reducing conditions on 4–15% Tris–HCl SDS-PAGE, and transferred to nitrocellulose membranes. Immunodetection was performed with (A) anti-type IV collagen (Col IV), (B) anti-laminin, (C) anti-nidogen 1, (D) anti-type I collagen (Col I), (E) anti-type VI collagen (Col VI), and (F) anti-fibronectin. The reaction was detected using an anti-rabbit peroxidase antibody and a chemiluminescent substrate. Images were obtained with the ChemiDoc XRS+ System (BioRad).

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Muscle Tissue Damage Induced by the Venom of Bothrops asper: Identification of Early and Late Pathological Events through Proteomic Analysis

    doi: 10.1371/journal.pntd.0004599

    Figure Lengend Snippet: Western blot analysis of extracellular matrix components in wound exudates collected from mice after injection of B . asper venom. Groups of five mice were injected in the gastrocnemius with 50 μg of B . asper venom. After 1, 6 and 24 h of injection mice were sacrificed and samples of exudates were collected, pooled and lyophilized. Afterwards, 100 μg of protein of each sample were separated under reducing conditions on 4–15% Tris–HCl SDS-PAGE, and transferred to nitrocellulose membranes. Immunodetection was performed with (A) anti-type IV collagen (Col IV), (B) anti-laminin, (C) anti-nidogen 1, (D) anti-type I collagen (Col I), (E) anti-type VI collagen (Col VI), and (F) anti-fibronectin. The reaction was detected using an anti-rabbit peroxidase antibody and a chemiluminescent substrate. Images were obtained with the ChemiDoc XRS+ System (BioRad).

    Article Snippet: Images were captured with the ChemiDoc XRS+ System (BioRad) and the analysis was performed with the ImageLab software (BioRad).

    Techniques: Western Blot, Mouse Assay, Injection, SDS Page, Immunodetection

    Western blot analysis of placenta homogenates using anti-ALDH3B2 and anti-GAPDH antibodies revealed bands corresponding to the molecular weight of 53 kDa (long isoform of ALDH3B2) and bands corresponding to molecular weight of 37 kDa (GAPDH). Proteins in homogenates were applied onto the polyacrylamide gel. After separation, they were transferred onto the PVDF membrane. Immunodetection of ALDH3B2 protein was performed using anti-ALDH3B2 antibody. After membrane stripping, the immunodetection was repeated with anti-GAPDH antibody. GAPDH detection was used as a loading control, as GAPDH gene is constitutively expressed at high levels in many tissues. The image was taken using ChemiDoc XRS+ (Bio-Rad). Additionally, the image of the whole membrane was added into the Supplementary Materials (Supplementary Figure S1) .

    Journal: International Journal of Molecular Sciences

    Article Title: Detection of ALDH3B2 in Human Placenta

    doi: 10.3390/ijms20246292

    Figure Lengend Snippet: Western blot analysis of placenta homogenates using anti-ALDH3B2 and anti-GAPDH antibodies revealed bands corresponding to the molecular weight of 53 kDa (long isoform of ALDH3B2) and bands corresponding to molecular weight of 37 kDa (GAPDH). Proteins in homogenates were applied onto the polyacrylamide gel. After separation, they were transferred onto the PVDF membrane. Immunodetection of ALDH3B2 protein was performed using anti-ALDH3B2 antibody. After membrane stripping, the immunodetection was repeated with anti-GAPDH antibody. GAPDH detection was used as a loading control, as GAPDH gene is constitutively expressed at high levels in many tissues. The image was taken using ChemiDoc XRS+ (Bio-Rad). Additionally, the image of the whole membrane was added into the Supplementary Materials (Supplementary Figure S1) .

    Article Snippet: Human ALDH3B2 protein was detected using goat anti-human ALDH3B2 antibody (1:200, v/v ) (Santa Cruz Biotechnology, Inc., Dallas, TX, USA) by incubation at 4 °C for 12 h. After being washed three times, membranes were incubated with horseradish peroxidase-conjugated (HRP) anti-goat antibody (1:40,000) (Sigma-Aldrich) for 1 h. Finally, proteins bound with antibody were visualized using chemiluminescence (Pierce ECL Western Blotting Substrate, Thermo Scientific, Waltham, MA, USA) and ChemiDoc XRS+ imaging system (Bio-Rad).

    Techniques: Western Blot, Molecular Weight, Immunodetection, Stripping Membranes

    Western blot analysis using anti-ALDH3B2 antibody confirmed the expression of both short and long isoforms of recombinant ALDH3B2 protein in E. coli . Purified recombinant proteins were applied onto the polyacrylamide gel. After separation, they were transferred onto the PVDF membrane. Their immunodetection was performed using anti-ALDH3B2 antibody. The image was taken using ChemiDoc XRS+ (Bio-Rad).

    Journal: International Journal of Molecular Sciences

    Article Title: Detection of ALDH3B2 in Human Placenta

    doi: 10.3390/ijms20246292

    Figure Lengend Snippet: Western blot analysis using anti-ALDH3B2 antibody confirmed the expression of both short and long isoforms of recombinant ALDH3B2 protein in E. coli . Purified recombinant proteins were applied onto the polyacrylamide gel. After separation, they were transferred onto the PVDF membrane. Their immunodetection was performed using anti-ALDH3B2 antibody. The image was taken using ChemiDoc XRS+ (Bio-Rad).

    Article Snippet: Human ALDH3B2 protein was detected using goat anti-human ALDH3B2 antibody (1:200, v/v ) (Santa Cruz Biotechnology, Inc., Dallas, TX, USA) by incubation at 4 °C for 12 h. After being washed three times, membranes were incubated with horseradish peroxidase-conjugated (HRP) anti-goat antibody (1:40,000) (Sigma-Aldrich) for 1 h. Finally, proteins bound with antibody were visualized using chemiluminescence (Pierce ECL Western Blotting Substrate, Thermo Scientific, Waltham, MA, USA) and ChemiDoc XRS+ imaging system (Bio-Rad).

    Techniques: Western Blot, Expressing, Recombinant, Purification, Immunodetection