chemical competent top10 cells  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 87

    Structured Review

    Thermo Fisher chemical competent top10 cells
    Chemical Competent Top10 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chemical competent top10 cells/product/Thermo Fisher
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chemical competent top10 cells - by Bioz Stars, 2020-04
    87/100 stars

    Images

    Related Articles

    Electroporation:

    Article Title: Construction of Gene Targeting Vectors by Recombineering
    Article Snippet: .. Add 5 μl of ligation products to chemical competent TOP10 cells (Invitrogen) and incubate on ice for 30 min (can use electroporation for the transformation). .. Heat shock at 42°C without shaking for 30 sec. Add 250 μl of SOC and incubate transformation mixture at 32°C for 1 hour.

    Agarose Gel Electrophoresis:

    Article Title: Construction of Gene Targeting Vectors by Recombineering
    Article Snippet: Run the restriction digestion reaction of PL613 through a 1.0% agarose gel, purify the lacZ reporter cassette (the 7 kb band) using QIAquick Gel Extraction kit (Qiagen). .. Add 5 μl of ligation products to chemical competent TOP10 cells (Invitrogen) and incubate on ice for 30 min (can use electroporation for the transformation).

    Ligation:

    Article Title: Construction of Gene Targeting Vectors by Recombineering
    Article Snippet: .. Add 5 μl of ligation products to chemical competent TOP10 cells (Invitrogen) and incubate on ice for 30 min (can use electroporation for the transformation). .. Heat shock at 42°C without shaking for 30 sec. Add 250 μl of SOC and incubate transformation mixture at 32°C for 1 hour.

    Size-exclusion Chromatography:

    Article Title: Construction of Gene Targeting Vectors by Recombineering
    Article Snippet: Add 5 μl of ligation products to chemical competent TOP10 cells (Invitrogen) and incubate on ice for 30 min (can use electroporation for the transformation). .. Heat shock at 42°C without shaking for 30 sec. Add 250 μl of SOC and incubate transformation mixture at 32°C for 1 hour.

    Purification:

    Article Title: Construction of Gene Targeting Vectors by Recombineering
    Article Snippet: Set up ligation reaction of purified digestion products using 12 μl of lacZ reporter cassette (600 ng), 10 μl of purified retrieved plasmid (50 ng), 2.5 μl of T4 DNA ligase buffer with 1.0 μl of T4 DNA ligase (New England Biolabs) and incubate at room temperature for 2 hours ( see ). .. Add 5 μl of ligation products to chemical competent TOP10 cells (Invitrogen) and incubate on ice for 30 min (can use electroporation for the transformation).

    Polymerase Chain Reaction:

    Article Title: Construction of Gene Targeting Vectors by Recombineering
    Article Snippet: Purify the restriction digested retrieved plasmid using Qiaprep Mini-preparation columns (Qiagen) and elute DNA in 30 μl of PCR-grade water ( See ). .. Add 5 μl of ligation products to chemical competent TOP10 cells (Invitrogen) and incubate on ice for 30 min (can use electroporation for the transformation).

    Gel Extraction:

    Article Title: Construction of Gene Targeting Vectors by Recombineering
    Article Snippet: Run the restriction digestion reaction of PL613 through a 1.0% agarose gel, purify the lacZ reporter cassette (the 7 kb band) using QIAquick Gel Extraction kit (Qiagen). .. Add 5 μl of ligation products to chemical competent TOP10 cells (Invitrogen) and incubate on ice for 30 min (can use electroporation for the transformation).

    Transformation Assay:

    Article Title: Construction of Gene Targeting Vectors by Recombineering
    Article Snippet: .. Add 5 μl of ligation products to chemical competent TOP10 cells (Invitrogen) and incubate on ice for 30 min (can use electroporation for the transformation). .. Heat shock at 42°C without shaking for 30 sec. Add 250 μl of SOC and incubate transformation mixture at 32°C for 1 hour.

    Plasmid Preparation:

    Article Title: Construction of Gene Targeting Vectors by Recombineering
    Article Snippet: Set up ligation reaction of purified digestion products using 12 μl of lacZ reporter cassette (600 ng), 10 μl of purified retrieved plasmid (50 ng), 2.5 μl of T4 DNA ligase buffer with 1.0 μl of T4 DNA ligase (New England Biolabs) and incubate at room temperature for 2 hours ( see ). .. Add 5 μl of ligation products to chemical competent TOP10 cells (Invitrogen) and incubate on ice for 30 min (can use electroporation for the transformation).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher chemical competent escherichia coli cells
    Chemical Competent Escherichia Coli Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chemical competent escherichia coli cells/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chemical competent escherichia coli cells - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results