Structured Review

Nacalai chemi lumi
Chemi Lumi, supplied by Nacalai, used in various techniques. Bioz Stars score: 99/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 40 article reviews
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chemi lumi - by Bioz Stars, 2020-01
99/100 stars

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Related Articles

Centrifugation:

Article Title: Human LYPD8 protein inhibits motility of flagellated bacteria
Article Snippet: Immunoreactivity was detected using Chemi-Lumi One (Nacalai Tesque). .. Immunoreactivity was detected using Chemi-Lumi One (Nacalai Tesque).

Polyacrylamide Gel Electrophoresis:

Article Title: F-fucoidan from Saccharina japonica is a novel inducer of galectin-9 and exhibits anti-allergic activity
Article Snippet: Samples were subjected to sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene fluoride membranes (General Electric, Fairfield, CT). .. Anti-mouse galectin-9 or anti-mouse IgE antibodies were reacted as the primary antibody at 4°C overnight, followed by anti-goat IgG-HRP antibody as the secondary antibody at 4°C for 1 h. All signals were detected by enhanced chemiluminescence using Chemi-Lumi One (Nacalai Tesque).

MTT Assay:

Article Title: Induction of Apoptosis in MCF-7 Cells via Oxidative Stress Generation, Mitochondria-Dependent and Caspase-Independent Pathway by Ethyl Acetate Extract of Dillenia suffruticosa and Its Chemical Profile
Article Snippet: 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT), cholera toxin, human insulin, epidermal growth factor and hydrocortisone were purchased from Sigma (St. Loius, USA). .. Phosphatase inhibitor cocktails, bovine serum albumin (BSA) and Chemi-Lumi One L were purchased from Nacalai Tesque (Kyoto, Japan).

Blocking Assay:

Article Title: Highly water pressurized brown rice improves cognitive dysfunction in senescence-accelerated mouse prone 8 and reduces amyloid beta in the brain
Article Snippet: After separation, the proteins were transferred onto a 0.45 μm polyvinylidene difluoride membrane (Merck Millipore), which was run at 15 V for 30 min. After blocking with 2.5% skim milk (Nacalai Tesque, Inc.) in TBS-T (Sigma-Aldrich Corp.) for 1 h, the blots were incubated with anti-APP (1:200 dilution; Immuno-Biological Laboratories Co., Ltd., Fujioka, Japan) or anti-actin (1:5000 dilution, Cell Signaling Technology, Inc. Danvers, MA, USA) primary antibodies at 4 °C overnight. .. The blots were washed thrice with TBS-T for 10 min before incubation with HRP-conjugated anti-rabbit immunoglobulin G secondary antibody (1:3000 dilution; GE Healthcare Little Chalfont, Buckinghamshire, UK) for 2 h at room temperature and washed thrice with TBS-T for 10 min. APP and action were detected using Chemi-Lumi One (Nacalai Tesque, Inc.), and the protein bands were analyzed with an ImageQuant LAS4000 (GE Healthcare) reader.

Article Title: Novel method for the high-throughput production of phosphorylation site-specific monoclonal antibodies
Article Snippet: Restriction enzymes and lambda protein phosphatase were purchased from New England BioLabs ( http://www.nebj ). .. Blocking One-P and Chemi-Lumi One Super were purchased from Nacalai Tesque ( http://www.nacalai.co.jp ). .. Synthetic peptides and the anti-pan-CHK2 antibody (DCS-273) were purchased from Medical & Biological Laboratories ( https://ruo.mbl.co.jp ).

Article Title: A Synthetic Analogue of Neopeltolide, 8,9-Dehydroneopeltolide, Is a Potent Anti-Austerity Agent against Starved Tumor Cells
Article Snippet: The membrane was blocked with Blocking One (Nacalai Tesque, Kyoto, Japan) at room temperature for 30 min, rinsed with T-TBS (×3), and probed with an appropriate primary antibody at room temperature for 1 h or at 4 °C overnight. .. The membrane was rinsed with T-TBS (×3) and probed with an appropriate HRP-conjugated secondary antibody at room temperature for 1 h. The membrane was rinsed with T-TBS (5 min × 3) and developed with Chemi-Lumi One (Nacalai Tesque, Kyoto, Japan).

Article Title: Chemical labelling for visualizing native AMPA receptors in live neurons
Article Snippet: The samples were applied to SDS-PAGE and electrotransferred onto immune-blot polyvinylidene fluoride (PVDF) membranes (Bio-Rad), followed by blocking with 5% nonfat dry milk in Tris-buffered saline (TBS) containing 0.05% Tween (Sigma-Aldrich). .. The signals were developed with Chemi-Lumi One (Nacalai tesque) or ECL Prime Western Blotting Detection Reagent (GE Healthcare) and detected with Imagequant LAS4000 (GE Healthcare).

Article Title: Chronic endometritis modifies decidualization in human endometrial stromal cells
Article Snippet: Membranes were incubated with 20% blocking reagent-N102 (NOF Co.) in 25 mM TBS containing 0.1% Tween-20 (TBST; pH 7.4) at room temperature for 1 h to block non-specific protein binding sites, followed by incubation with the IGFBP-1 antibody (1: 1000; Santa Cruz Biotechnology, Inc.) or GAPDH (1:5000; Enzo Life Science, Inc.) diluted by TBST at 4 °C overnight. .. Peroxidase labeling was detected by chemiluminescence using the Chemi-Lumi one super (Nacalai Tesque).

Article Title: Metformin treatment ameliorates diabetes‐associated decline in hippocampal neurogenesis and memory via phosphorylation of insulin receptor substrate 1
Article Snippet: Membranes were blocked using 4% block ace (Yukijirushi Co., Tokyo, Japan) at room temperature for 1 h, incubated with the indicated primary antibodies at 4 °C overnight, followed by incubation with horseradish peroxidase‐conjugated secondary antibodies at room temperature for 2 h. Primary antibodies were rabbit anti‐phospho‐IR β (Tyr1135/Tyr1136; 1 : 1000; Cell Signaling Technology, Danvers, MA, USA), rabbit anti‐IR β (1 : 1000; Cell Signaling Technology), rabbit anti‐phospho‐IRS1 [mouse Ser307/human Ser312 (mSer307/hSer312), mouse Ser612/human Ser616 (mSer612/hSer616), mouse Ser632/Ser635/human Ser636/Ser639 (mSer632/Ser635/hSer636/Ser639), mouse Ser1097/human Ser1101 (mSer1097/hSer1101)] (1 : 500; Cell Signaling Technology), rabbit anti‐IRS1 (1 : 1000; Cell Signaling Technology), rabbit anti‐phospho‐AMPK (Thr172; 1 : 1000; Cell Signaling Technology), rabbit anti‐AMPK (1 : 1000; Cell Signaling Technology), rabbit anti‐phospho‐aPKC ζ/λ (Thr410/Thr403; 1 : 500; Cell Signaling Technology), rabbit anti‐aPKC λ/ι (1 : 500; Santa Cruz Biotechnology), rabbit anti‐phospho‐mTOR (Ser2448; 1 : 1000; Cell Signaling Technology), rabbit anti‐mTOR (1 : 1000; Cell Signaling Technology), and rabbit anti‐β‐tubulin (1 : 1000; Cell Signaling Technology). .. The chemiluminescent signals were detected using Chemi‐Lumi One (Nacalai Tesque) or ImmunoStar LD (Wako).

Enzyme-linked Immunosorbent Assay:

Article Title: Methylation and Acetylation Enhanced the Antidiabetic Activity of Some Selected Flavonoids: In Vitro, Molecular Modelling and Structure Activity Relationship-Based Study
Article Snippet: An insulin secretion enzyme-linked immunosorbent assay (ELISA) kit was purchased from Elabscience, Wuhan, China. .. Chemiluminescence, Chemi-Lumi One L and Tween-20 were bought from Nacalai-tesque, Kyoto, Japan.

Incubation:

Article Title: Human LYPD8 protein inhibits motility of flagellated bacteria
Article Snippet: The supernatant and pellet suspension were separated with SDS-PAGE and transferred to polyvinylidene fluoride membranes (Millipore) that were incubated with HRP-conjugated anti-FLAG M2 mAb (Sigma-Aldrich). .. Immunoreactivity was detected using Chemi-Lumi One (Nacalai Tesque).

Article Title: Highly water pressurized brown rice improves cognitive dysfunction in senescence-accelerated mouse prone 8 and reduces amyloid beta in the brain
Article Snippet: After separation, the proteins were transferred onto a 0.45 μm polyvinylidene difluoride membrane (Merck Millipore), which was run at 15 V for 30 min. After blocking with 2.5% skim milk (Nacalai Tesque, Inc.) in TBS-T (Sigma-Aldrich Corp.) for 1 h, the blots were incubated with anti-APP (1:200 dilution; Immuno-Biological Laboratories Co., Ltd., Fujioka, Japan) or anti-actin (1:5000 dilution, Cell Signaling Technology, Inc. Danvers, MA, USA) primary antibodies at 4 °C overnight. .. The blots were washed thrice with TBS-T for 10 min before incubation with HRP-conjugated anti-rabbit immunoglobulin G secondary antibody (1:3000 dilution; GE Healthcare Little Chalfont, Buckinghamshire, UK) for 2 h at room temperature and washed thrice with TBS-T for 10 min. APP and action were detected using Chemi-Lumi One (Nacalai Tesque, Inc.), and the protein bands were analyzed with an ImageQuant LAS4000 (GE Healthcare) reader. .. The band density of APP was corrected by comparing it with the band intensity of actin.

Article Title: F-fucoidan from Saccharina japonica is a novel inducer of galectin-9 and exhibits anti-allergic activity
Article Snippet: Anti-TNP IgE (50 ng) was added and left on ice for 15 min. Then, the mixtures were incubated with anti-mouse IgE antibodies bound to protein G Dynabeads (Life Technologies) at room temperature for 10 min. SDS sample buffer (62.5 mM Tris-HCl (pH 6.8), 1% SDS, 10% glycerol, 0.005% bromophenol blue, and 2-mercaptoethanol) was added to each of the supernatants and beads, then heated at 70°C for 10 min. .. Anti-mouse galectin-9 or anti-mouse IgE antibodies were reacted as the primary antibody at 4°C overnight, followed by anti-goat IgG-HRP antibody as the secondary antibody at 4°C for 1 h. All signals were detected by enhanced chemiluminescence using Chemi-Lumi One (Nacalai Tesque).

Article Title: Separate Cellular Localizations of Human T-Lymphotropic Virus 1 (HTLV-1) Env and Glucose Transporter Type 1 (GLUT1) Are Required for HTLV-1 Env-Mediated Fusion and Infection
Article Snippet: The membranes were incubated with anti-gp46 rat MAb (LAT-27), anti-FLAG mouse MAb (Wako, Osaka, Japan), anti-HA mouse MAb (Wako), anti-HIV-1 Gag mouse MAb (24-3) , anti-cyclophilin A (CypA) rabbit polyclonal antibody (Enzo Life Sciences, Farmingdale, NY, USA), or anti-β-actin mouse MAb (Sigma), followed by staining with horseradish peroxidase (HRP)-conjugated anti-mouse (Jackson ImmunoResearch, West Grove, PA, USA), anti-rat (Jackson ImmunoResearch), or anti-rabbit (Jackson ImmunoResearch) IgG. .. The signals were detected using Chemi-Lumi One (Nacalai Tesque).

Article Title: Cyclocarya paliurus extract activates insulin signaling via Sirtuin1 in C2C12 myotubes and decreases blood glucose level in mice with impaired insulin secretion
Article Snippet: The membrane was blocked, and the blot was incubated successively with the primary and secondary antibodies. .. Finally, detection was achieved using Chemi-Lumi one super (Nacalai Tesque, Kyoto).

Article Title: Learning and Memory Deficits in Male Adult Mice Treated with a Benzodiazepine Sleep-Inducing Drug during the Juvenile Period
Article Snippet: The membranes were then incubated with peroxidase-conjugated secondary antibodies for 2 h at room temperature. .. After several PBS-T washes, the membranes were incubated with chemi-lumi one L (Nacalai Tesque, Inc., Kyoto, Japan) for signal production. .. The signal was photographed using an LAS-3000 (Fujifilm Co., Ltd., Tokyo, Japan).

Article Title: Cellular and viral chromatin proteins are positive factors in the regulation of adenovirus gene expression
Article Snippet: After washing with TBST, the membranes were incubated with antimouse or rabbit IgG antibodies conjugated with horseradish peroxidase (GE healthcare) at room temperature, followed by washing. .. The blots were developed using Chemi-Lumi One (Nacalai tesque).

Article Title: Chronic endometritis modifies decidualization in human endometrial stromal cells
Article Snippet: Membranes were incubated with 20% blocking reagent-N102 (NOF Co.) in 25 mM TBS containing 0.1% Tween-20 (TBST; pH 7.4) at room temperature for 1 h to block non-specific protein binding sites, followed by incubation with the IGFBP-1 antibody (1: 1000; Santa Cruz Biotechnology, Inc.) or GAPDH (1:5000; Enzo Life Science, Inc.) diluted by TBST at 4 °C overnight. .. Peroxidase labeling was detected by chemiluminescence using the Chemi-Lumi one super (Nacalai Tesque).

Article Title: Metformin treatment ameliorates diabetes‐associated decline in hippocampal neurogenesis and memory via phosphorylation of insulin receptor substrate 1
Article Snippet: Membranes were blocked using 4% block ace (Yukijirushi Co., Tokyo, Japan) at room temperature for 1 h, incubated with the indicated primary antibodies at 4 °C overnight, followed by incubation with horseradish peroxidase‐conjugated secondary antibodies at room temperature for 2 h. Primary antibodies were rabbit anti‐phospho‐IR β (Tyr1135/Tyr1136; 1 : 1000; Cell Signaling Technology, Danvers, MA, USA), rabbit anti‐IR β (1 : 1000; Cell Signaling Technology), rabbit anti‐phospho‐IRS1 [mouse Ser307/human Ser312 (mSer307/hSer312), mouse Ser612/human Ser616 (mSer612/hSer616), mouse Ser632/Ser635/human Ser636/Ser639 (mSer632/Ser635/hSer636/Ser639), mouse Ser1097/human Ser1101 (mSer1097/hSer1101)] (1 : 500; Cell Signaling Technology), rabbit anti‐IRS1 (1 : 1000; Cell Signaling Technology), rabbit anti‐phospho‐AMPK (Thr172; 1 : 1000; Cell Signaling Technology), rabbit anti‐AMPK (1 : 1000; Cell Signaling Technology), rabbit anti‐phospho‐aPKC ζ/λ (Thr410/Thr403; 1 : 500; Cell Signaling Technology), rabbit anti‐aPKC λ/ι (1 : 500; Santa Cruz Biotechnology), rabbit anti‐phospho‐mTOR (Ser2448; 1 : 1000; Cell Signaling Technology), rabbit anti‐mTOR (1 : 1000; Cell Signaling Technology), and rabbit anti‐β‐tubulin (1 : 1000; Cell Signaling Technology). .. The chemiluminescent signals were detected using Chemi‐Lumi One (Nacalai Tesque) or ImmunoStar LD (Wako).

Expressing:

Article Title: Learning and Memory Deficits in Male Adult Mice Treated with a Benzodiazepine Sleep-Inducing Drug during the Juvenile Period
Article Snippet: After several PBS-T washes, the membranes were incubated with chemi-lumi one L (Nacalai Tesque, Inc., Kyoto, Japan) for signal production. .. After several PBS-T washes, the membranes were incubated with chemi-lumi one L (Nacalai Tesque, Inc., Kyoto, Japan) for signal production.

Article Title: Cellular and viral chromatin proteins are positive factors in the regulation of adenovirus gene expression
Article Snippet: For checking the expression level of TAF-I and β-actin, cell lysates were prepared using lysis buffer for ChIP assays. .. The blots were developed using Chemi-Lumi One (Nacalai tesque).

BIA-KA:

Article Title: Metformin treatment ameliorates diabetes‐associated decline in hippocampal neurogenesis and memory via phosphorylation of insulin receptor substrate 1
Article Snippet: Protein concentration was calculated using BCA protein assay kit (Pierce, Rockford, IL, USA). .. The chemiluminescent signals were detected using Chemi‐Lumi One (Nacalai Tesque) or ImmunoStar LD (Wako).

Acrylamide Gel Assay:

Article Title: Methylation and Acetylation Enhanced the Antidiabetic Activity of Some Selected Flavonoids: In Vitro, Molecular Modelling and Structure Activity Relationship-Based Study
Article Snippet: For Western blotting, 10% SDS TGX stain free™ FastCast acrylamide gel kits, Immun-Blot® polyvinylidene difluoride (PVDF), non-fat dried milk were purchased from Bio-Rad, Santa Rosa, CA, USA. .. Chemiluminescence, Chemi-Lumi One L and Tween-20 were bought from Nacalai-tesque, Kyoto, Japan.

Western Blot:

Article Title: Hypoxia‐inducible factor‐targeting prodrug TOP3 combined with gemcitabine or TS‐1 improves pancreatic cancer survival in an orthotopic model
Article Snippet: Paragraph title: Western blot analysis ... Binding was detected with Chemi‐Lumi One Ultra, a chemiluminescence detection reagent (Nacalai Tesque).

Article Title: Highly water pressurized brown rice improves cognitive dysfunction in senescence-accelerated mouse prone 8 and reduces amyloid beta in the brain
Article Snippet: The level of amyloid precursor protein (APP) in the brain was estimated western blotting. .. The blots were washed thrice with TBS-T for 10 min before incubation with HRP-conjugated anti-rabbit immunoglobulin G secondary antibody (1:3000 dilution; GE Healthcare Little Chalfont, Buckinghamshire, UK) for 2 h at room temperature and washed thrice with TBS-T for 10 min. APP and action were detected using Chemi-Lumi One (Nacalai Tesque, Inc.), and the protein bands were analyzed with an ImageQuant LAS4000 (GE Healthcare) reader.

Article Title: F-fucoidan from Saccharina japonica is a novel inducer of galectin-9 and exhibits anti-allergic activity
Article Snippet: Paragraph title: Immunoprecipitation, SDS-PAGE and western blotting ... Anti-mouse galectin-9 or anti-mouse IgE antibodies were reacted as the primary antibody at 4°C overnight, followed by anti-goat IgG-HRP antibody as the secondary antibody at 4°C for 1 h. All signals were detected by enhanced chemiluminescence using Chemi-Lumi One (Nacalai Tesque).

Article Title: Methylation and Acetylation Enhanced the Antidiabetic Activity of Some Selected Flavonoids: In Vitro, Molecular Modelling and Structure Activity Relationship-Based Study
Article Snippet: For Western blotting, 10% SDS TGX stain free™ FastCast acrylamide gel kits, Immun-Blot® polyvinylidene difluoride (PVDF), non-fat dried milk were purchased from Bio-Rad, Santa Rosa, CA, USA. .. Chemiluminescence, Chemi-Lumi One L and Tween-20 were bought from Nacalai-tesque, Kyoto, Japan.

Article Title: Separate Cellular Localizations of Human T-Lymphotropic Virus 1 (HTLV-1) Env and Glucose Transporter Type 1 (GLUT1) Are Required for HTLV-1 Env-Mediated Fusion and Infection
Article Snippet: Paragraph title: Immunoprecipitation and Western blotting. ... The signals were detected using Chemi-Lumi One (Nacalai Tesque).

Article Title: Differentiation potential of Pluripotent Stem Cells correlates to the level of CHD7
Article Snippet: Paragraph title: Western Blotting for detection of CHD7 ... The signal was detected with Chemi-Lumi One Super reagents (Nacalai Tesque).

Article Title: Cyclocarya paliurus extract activates insulin signaling via Sirtuin1 in C2C12 myotubes and decreases blood glucose level in mice with impaired insulin secretion
Article Snippet: Paragraph title: Western blotting ... Finally, detection was achieved using Chemi-Lumi one super (Nacalai Tesque, Kyoto).

Article Title: Rapid labelling and covalent inhibition of intracellular native proteins using ligand-directed N-acyl-N-alkyl sulfonamide
Article Snippet: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting were carried out with a Bio-Rad Mini-Protean III electrophoresis apparatus. .. Fluorescence gel images and chemical luminescent signals using Chemi-Lumi one (Nacalai Tesque) or ECL Prime (GE Healthcare) were obtained by an imagequant LAS4000 (Fujifilm).

Article Title: Learning and Memory Deficits in Male Adult Mice Treated with a Benzodiazepine Sleep-Inducing Drug during the Juvenile Period
Article Snippet: Paragraph title: Western blotting ... After several PBS-T washes, the membranes were incubated with chemi-lumi one L (Nacalai Tesque, Inc., Kyoto, Japan) for signal production.

Article Title: Chemical labelling for visualizing native AMPA receptors in live neurons
Article Snippet: The immunodetection of GluA2 was performed with a rabbit anti-GluA2/3 antibody (Millipore, 07-598, × 3,000) and anti-rabbit IgG-HRP conjugate (Santa Cruz, sc-2004 × 3,000). .. The signals were developed with Chemi-Lumi One (Nacalai tesque) or ECL Prime Western Blotting Detection Reagent (GE Healthcare) and detected with Imagequant LAS4000 (GE Healthcare). .. Production, refolding and purification of a ligand binding domain of GluA2 (S1S2J) were carried out as previously described (The expression plasmid was kindly gifted from Professor Gouaux).

Article Title: Chemical labelling for visualizing native AMPA receptors in live neurons
Article Snippet: SDS–PAGE and western blotting were carried out using a Bio-Rad Mini-Protean III electrophoresis apparatus. .. Chemiluminescent signals generated with Chemi-Lumi One (nacalai tesque) or ECL Prime (GE Healthcare) were detected with an LAS4000 imaging system (Fuji Film).

Article Title: Cellular and viral chromatin proteins are positive factors in the regulation of adenovirus gene expression
Article Snippet: Paragraph title: Western blotting ... The blots were developed using Chemi-Lumi One (Nacalai tesque).

Article Title: Chronic endometritis modifies decidualization in human endometrial stromal cells
Article Snippet: Paragraph title: Protein extraction from cultured cells and western blotting ... Peroxidase labeling was detected by chemiluminescence using the Chemi-Lumi one super (Nacalai Tesque).

Article Title: Metformin treatment ameliorates diabetes‐associated decline in hippocampal neurogenesis and memory via phosphorylation of insulin receptor substrate 1
Article Snippet: Paragraph title: Western blotting ... The chemiluminescent signals were detected using Chemi‐Lumi One (Nacalai Tesque) or ImmunoStar LD (Wako).

High Performance Liquid Chromatography:

Article Title: Rapid labelling and covalent inhibition of intracellular native proteins using ligand-directed N-acyl-N-alkyl sulfonamide
Article Snippet: Fluorescence gel images and chemical luminescent signals using Chemi-Lumi one (Nacalai Tesque) or ECL Prime (GE Healthcare) were obtained by an imagequant LAS4000 (Fujifilm). .. UV-vis absorption spectra were obtained with a Shimadzu UV-2550 spectrophotometer.

Flow Cytometry:

Article Title: Rapid labelling and covalent inhibition of intracellular native proteins using ligand-directed N-acyl-N-alkyl sulfonamide
Article Snippet: Fluorescence gel images and chemical luminescent signals using Chemi-Lumi one (Nacalai Tesque) or ECL Prime (GE Healthcare) were obtained by an imagequant LAS4000 (Fujifilm). .. UV-vis absorption spectra were obtained with a Shimadzu UV-2550 spectrophotometer.

Protease Inhibitor:

Article Title: Separate Cellular Localizations of Human T-Lymphotropic Virus 1 (HTLV-1) Env and Glucose Transporter Type 1 (GLUT1) Are Required for HTLV-1 Env-Mediated Fusion and Infection
Article Snippet: The cells then were solubilized using 1% Brij O10 (Sigma-Aldrich) lysis buffer (1% Briji O10, 20 mM Tris-Cl, pH 8.0, 150 mM NaCl) with a protease inhibitor cocktail (Nakalai Tesque, Kyoto, Japan). .. The signals were detected using Chemi-Lumi One (Nacalai Tesque).

Article Title: Differentiation potential of Pluripotent Stem Cells correlates to the level of CHD7
Article Snippet: Proteins were extracted using Complete Lysis-M (Roche), supplemented with protease inhibitor cocktail tablets (Complete Mini; Roche). .. The signal was detected with Chemi-Lumi One Super reagents (Nacalai Tesque).

Article Title: Induction of Apoptosis in MCF-7 Cells via Oxidative Stress Generation, Mitochondria-Dependent and Caspase-Independent Pathway by Ethyl Acetate Extract of Dillenia suffruticosa and Its Chemical Profile
Article Snippet: Phenylmethanesulfonyl fluoride (PMSF) and protease inhibitor cocktails were purchased from Calbiochem (California, USA). .. Phosphatase inhibitor cocktails, bovine serum albumin (BSA) and Chemi-Lumi One L were purchased from Nacalai Tesque (Kyoto, Japan).

Article Title: Chemical labelling for visualizing native AMPA receptors in live neurons
Article Snippet: Fluorescence images were acquired using a 488 nm line of an argon laser for excitation of OG and a HeNe Green laser for excitation of mCherry-F. For western blot analysis, after chemical labelling, cells were washed 3 times with ice-cold HBS, lysed with radio immunoprecipitation assay (RIPA) buffer containing 1% protease inhibitor cocktail set III (Calbiochem), and mixed with a quarter volume of 5 × SDS–PAGE loading buffer containing 250 mM DTT. .. The signals were developed with Chemi-Lumi One (Nacalai tesque) or ECL Prime Western Blotting Detection Reagent (GE Healthcare) and detected with Imagequant LAS4000 (GE Healthcare).

Qubit Protein Assay:

Article Title: Learning and Memory Deficits in Male Adult Mice Treated with a Benzodiazepine Sleep-Inducing Drug during the Juvenile Period
Article Snippet: The amount of protein was quantified using Qubit protein assay kits (Life Technologies Co., California, USA). .. After several PBS-T washes, the membranes were incubated with chemi-lumi one L (Nacalai Tesque, Inc., Kyoto, Japan) for signal production.

Cell Culture:

Article Title: Hypoxia‐inducible factor‐targeting prodrug TOP3 combined with gemcitabine or TS‐1 improves pancreatic cancer survival in an orthotopic model
Article Snippet: Cells were seeded at 1 × 105 cells in 35‐mm dishes, pre‐incubated for 12 h in normoxia, and further cultured in hypoxia (1% O2 ) for the indicated time. .. Binding was detected with Chemi‐Lumi One Ultra, a chemiluminescence detection reagent (Nacalai Tesque).

Article Title: Separate Cellular Localizations of Human T-Lymphotropic Virus 1 (HTLV-1) Env and Glucose Transporter Type 1 (GLUT1) Are Required for HTLV-1 Env-Mediated Fusion and Infection
Article Snippet: The 293T cells in six-well plates were transfected with 3.5 μg of pNL43ΔBglII, 1.5 μg of pcDNA-1E-RRE, 0.5 μg of pCMV-rev, and 0.5 μg of pAdvantage (Promega) using Lipofectamine 2000 (Invitrogen) and then cultured for 24 h at 37°C. .. The signals were detected using Chemi-Lumi One (Nacalai Tesque).

Article Title: Chronic endometritis modifies decidualization in human endometrial stromal cells
Article Snippet: Paragraph title: Protein extraction from cultured cells and western blotting ... Peroxidase labeling was detected by chemiluminescence using the Chemi-Lumi one super (Nacalai Tesque).

Generated:

Article Title: Chemical labelling for visualizing native AMPA receptors in live neurons
Article Snippet: SDS–PAGE and western blotting were carried out using a Bio-Rad Mini-Protean III electrophoresis apparatus. .. Chemiluminescent signals generated with Chemi-Lumi One (nacalai tesque) or ECL Prime (GE Healthcare) were detected with an LAS4000 imaging system (Fuji Film). .. All experiment procedures were performed in accordance with the National Institute of Health Guide for the Care and Use of Laboratory Animals and approved by the Institutional Animal Use Committees of Kyoto University or Keio University.

other:

Article Title: Programmed Cell Death Induced by (−)-8,9-Dehydroneopeltolide in Human Promyelocytic Leukemia HL-60 Cells under Energy Stress Conditions
Article Snippet: The membrane was then probed with an appropriate HRP-conjugated secondary antibody at room temperature for 1 h and then developed with Chemi-Lumi One (Nacalai Tesque, Kyoto, Japan).

Imaging:

Article Title: Chemical labelling for visualizing native AMPA receptors in live neurons
Article Snippet: Cell imaging was performed with a confocal microscopy (FV1000, IX81, Olympus) equipped with a 60 × , numerical aperture (NA)=1.35 oil objective. .. The signals were developed with Chemi-Lumi One (Nacalai tesque) or ECL Prime Western Blotting Detection Reagent (GE Healthcare) and detected with Imagequant LAS4000 (GE Healthcare).

Article Title: Chemical labelling for visualizing native AMPA receptors in live neurons
Article Snippet: SDS–PAGE and western blotting were carried out using a Bio-Rad Mini-Protean III electrophoresis apparatus. .. Chemiluminescent signals generated with Chemi-Lumi One (nacalai tesque) or ECL Prime (GE Healthcare) were detected with an LAS4000 imaging system (Fuji Film). .. All experiment procedures were performed in accordance with the National Institute of Health Guide for the Care and Use of Laboratory Animals and approved by the Institutional Animal Use Committees of Kyoto University or Keio University.

Protein Concentration:

Article Title: Metformin treatment ameliorates diabetes‐associated decline in hippocampal neurogenesis and memory via phosphorylation of insulin receptor substrate 1
Article Snippet: Protein concentration was calculated using BCA protein assay kit (Pierce, Rockford, IL, USA). .. The chemiluminescent signals were detected using Chemi‐Lumi One (Nacalai Tesque) or ImmunoStar LD (Wako).

Recombinant:

Article Title: Human LYPD8 protein inhibits motility of flagellated bacteria
Article Snippet: Bacterial bodies or flagella were mixed with the solution of FLAG-tagged recombinant LYPD8 (10 ng/μl) and incubated for 3 h at 4 °C. .. Immunoreactivity was detected using Chemi-Lumi One (Nacalai Tesque).

Article Title: Novel method for the high-throughput production of phosphorylation site-specific monoclonal antibodies
Article Snippet: Cell Lysis/Binding buffer, Dynabeads Oligo(dT)25 , recombinant CD38 and DyLight Fluor Labeling Reagents were purchased from Thermo Fisher Scientific ( http://www.thermoscientific.com ). .. Blocking One-P and Chemi-Lumi One Super were purchased from Nacalai Tesque ( http://www.nacalai.co.jp ).

Article Title: F-fucoidan from Saccharina japonica is a novel inducer of galectin-9 and exhibits anti-allergic activity
Article Snippet: Recombinant mouse galectin-9 (250 ng) and lactose (100 mM) were mixed and left on ice for 15 min. Sucrose (100 mM) was used as a negative control. .. Anti-mouse galectin-9 or anti-mouse IgE antibodies were reacted as the primary antibody at 4°C overnight, followed by anti-goat IgG-HRP antibody as the secondary antibody at 4°C for 1 h. All signals were detected by enhanced chemiluminescence using Chemi-Lumi One (Nacalai Tesque).

Nucleic Acid Electrophoresis:

Article Title: Rapid labelling and covalent inhibition of intracellular native proteins using ligand-directed N-acyl-N-alkyl sulfonamide
Article Snippet: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting were carried out with a Bio-Rad Mini-Protean III electrophoresis apparatus. .. Fluorescence gel images and chemical luminescent signals using Chemi-Lumi one (Nacalai Tesque) or ECL Prime (GE Healthcare) were obtained by an imagequant LAS4000 (Fujifilm).

Pull Down Assay:

Article Title: Human LYPD8 protein inhibits motility of flagellated bacteria
Article Snippet: Paragraph title: Pull-down assay for LYPD8 binding to flagella ... Immunoreactivity was detected using Chemi-Lumi One (Nacalai Tesque).

Fluorescence:

Article Title: Rapid labelling and covalent inhibition of intracellular native proteins using ligand-directed N-acyl-N-alkyl sulfonamide
Article Snippet: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting were carried out with a Bio-Rad Mini-Protean III electrophoresis apparatus. .. Fluorescence gel images and chemical luminescent signals using Chemi-Lumi one (Nacalai Tesque) or ECL Prime (GE Healthcare) were obtained by an imagequant LAS4000 (Fujifilm). .. Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) spectra were measured with an Autoflex III, Ultraflex III or UltrafleXtreme (Bruker Daltonics) using α-cyano-4-hydroxycinnamic acid (CHCA) or sinapic acid as the matrix.

Article Title: Chemical labelling for visualizing native AMPA receptors in live neurons
Article Snippet: Fluorescence images were acquired using a 488 nm line of an argon laser for excitation of OG and a HeNe Green laser for excitation of mCherry-F. For western blot analysis, after chemical labelling, cells were washed 3 times with ice-cold HBS, lysed with radio immunoprecipitation assay (RIPA) buffer containing 1% protease inhibitor cocktail set III (Calbiochem), and mixed with a quarter volume of 5 × SDS–PAGE loading buffer containing 250 mM DTT. .. The signals were developed with Chemi-Lumi One (Nacalai tesque) or ECL Prime Western Blotting Detection Reagent (GE Healthcare) and detected with Imagequant LAS4000 (GE Healthcare).

Negative Control:

Article Title: F-fucoidan from Saccharina japonica is a novel inducer of galectin-9 and exhibits anti-allergic activity
Article Snippet: Recombinant mouse galectin-9 (250 ng) and lactose (100 mM) were mixed and left on ice for 15 min. Sucrose (100 mM) was used as a negative control. .. Anti-mouse galectin-9 or anti-mouse IgE antibodies were reacted as the primary antibody at 4°C overnight, followed by anti-goat IgG-HRP antibody as the secondary antibody at 4°C for 1 h. All signals were detected by enhanced chemiluminescence using Chemi-Lumi One (Nacalai Tesque).

Transfection:

Article Title: Separate Cellular Localizations of Human T-Lymphotropic Virus 1 (HTLV-1) Env and Glucose Transporter Type 1 (GLUT1) Are Required for HTLV-1 Env-Mediated Fusion and Infection
Article Snippet: For the preparation of VLPs, culture supernatants from transfected 293T cells were harvested after 24 h of culture and filtered through 0.45-μm-pore-size filters. .. The signals were detected using Chemi-Lumi One (Nacalai Tesque).

Labeling:

Article Title: Novel method for the high-throughput production of phosphorylation site-specific monoclonal antibodies
Article Snippet: Cell Lysis/Binding buffer, Dynabeads Oligo(dT)25 , recombinant CD38 and DyLight Fluor Labeling Reagents were purchased from Thermo Fisher Scientific ( http://www.thermoscientific.com ). .. Blocking One-P and Chemi-Lumi One Super were purchased from Nacalai Tesque ( http://www.nacalai.co.jp ).

Article Title: Chronic endometritis modifies decidualization in human endometrial stromal cells
Article Snippet: After washing with TBST for 20 min thrice, the membranes were reacted with a peroxidase-labeled anti-mouse IgG (1:50,000; GE Healthcare) for 1 h at room temperature. .. Peroxidase labeling was detected by chemiluminescence using the Chemi-Lumi one super (Nacalai Tesque). .. Total RNA was extracted from ESCs after culture for 13 days with RNeasy Micro kit (QIAGEN.) following the manufacturer’s instructions.

Purification:

Article Title: Novel method for the high-throughput production of phosphorylation site-specific monoclonal antibodies
Article Snippet: Blocking One-P and Chemi-Lumi One Super were purchased from Nacalai Tesque ( http://www.nacalai.co.jp ). .. Blocking One-P and Chemi-Lumi One Super were purchased from Nacalai Tesque ( http://www.nacalai.co.jp ).

Article Title: Rapid labelling and covalent inhibition of intracellular native proteins using ligand-directed N-acyl-N-alkyl sulfonamide
Article Snippet: All biological reagents were purchased from Sigma-Aldrich, Tokyo Chemical Industry (TCI), Wako Pure Chemical Industries, Sasaki Chemical, Bio-Rad, Thermo Fisher Scientific, Nacalai Tesque or Watanabe Chemical Industries, and used without further purification, unless otherwise noted. .. Fluorescence gel images and chemical luminescent signals using Chemi-Lumi one (Nacalai Tesque) or ECL Prime (GE Healthcare) were obtained by an imagequant LAS4000 (Fujifilm).

Protein Extraction:

Article Title: Highly water pressurized brown rice improves cognitive dysfunction in senescence-accelerated mouse prone 8 and reduces amyloid beta in the brain
Article Snippet: Paragraph title: Protein extraction from the brain tissue and measure amyloid-β ... The blots were washed thrice with TBS-T for 10 min before incubation with HRP-conjugated anti-rabbit immunoglobulin G secondary antibody (1:3000 dilution; GE Healthcare Little Chalfont, Buckinghamshire, UK) for 2 h at room temperature and washed thrice with TBS-T for 10 min. APP and action were detected using Chemi-Lumi One (Nacalai Tesque, Inc.), and the protein bands were analyzed with an ImageQuant LAS4000 (GE Healthcare) reader.

Article Title: Chronic endometritis modifies decidualization in human endometrial stromal cells
Article Snippet: Paragraph title: Protein extraction from cultured cells and western blotting ... Peroxidase labeling was detected by chemiluminescence using the Chemi-Lumi one super (Nacalai Tesque).

Confocal Microscopy:

Article Title: Chemical labelling for visualizing native AMPA receptors in live neurons
Article Snippet: Cell imaging was performed with a confocal microscopy (FV1000, IX81, Olympus) equipped with a 60 × , numerical aperture (NA)=1.35 oil objective. .. The signals were developed with Chemi-Lumi One (Nacalai tesque) or ECL Prime Western Blotting Detection Reagent (GE Healthcare) and detected with Imagequant LAS4000 (GE Healthcare).

Lysis:

Article Title: Novel method for the high-throughput production of phosphorylation site-specific monoclonal antibodies
Article Snippet: Cell Lysis/Binding buffer, Dynabeads Oligo(dT)25 , recombinant CD38 and DyLight Fluor Labeling Reagents were purchased from Thermo Fisher Scientific ( http://www.thermoscientific.com ). .. Blocking One-P and Chemi-Lumi One Super were purchased from Nacalai Tesque ( http://www.nacalai.co.jp ).

Article Title: Separate Cellular Localizations of Human T-Lymphotropic Virus 1 (HTLV-1) Env and Glucose Transporter Type 1 (GLUT1) Are Required for HTLV-1 Env-Mediated Fusion and Infection
Article Snippet: The cells then were solubilized using 1% Brij O10 (Sigma-Aldrich) lysis buffer (1% Briji O10, 20 mM Tris-Cl, pH 8.0, 150 mM NaCl) with a protease inhibitor cocktail (Nakalai Tesque, Kyoto, Japan). .. The signals were detected using Chemi-Lumi One (Nacalai Tesque).

Article Title: Differentiation potential of Pluripotent Stem Cells correlates to the level of CHD7
Article Snippet: Proteins were extracted using Complete Lysis-M (Roche), supplemented with protease inhibitor cocktail tablets (Complete Mini; Roche). .. The signal was detected with Chemi-Lumi One Super reagents (Nacalai Tesque).

Article Title: Cellular and viral chromatin proteins are positive factors in the regulation of adenovirus gene expression
Article Snippet: For checking the expression level of TAF-I and β-actin, cell lysates were prepared using lysis buffer for ChIP assays. .. The blots were developed using Chemi-Lumi One (Nacalai tesque).

Chromatin Immunoprecipitation:

Article Title: Cellular and viral chromatin proteins are positive factors in the regulation of adenovirus gene expression
Article Snippet: For checking the expression level of TAF-I and β-actin, cell lysates were prepared using lysis buffer for ChIP assays. .. The blots were developed using Chemi-Lumi One (Nacalai tesque).

SDS Page:

Article Title: Human LYPD8 protein inhibits motility of flagellated bacteria
Article Snippet: The supernatant and pellet suspension were separated with SDS-PAGE and transferred to polyvinylidene fluoride membranes (Millipore) that were incubated with HRP-conjugated anti-FLAG M2 mAb (Sigma-Aldrich). .. Immunoreactivity was detected using Chemi-Lumi One (Nacalai Tesque).

Article Title: F-fucoidan from Saccharina japonica is a novel inducer of galectin-9 and exhibits anti-allergic activity
Article Snippet: Paragraph title: Immunoprecipitation, SDS-PAGE and western blotting ... Anti-mouse galectin-9 or anti-mouse IgE antibodies were reacted as the primary antibody at 4°C overnight, followed by anti-goat IgG-HRP antibody as the secondary antibody at 4°C for 1 h. All signals were detected by enhanced chemiluminescence using Chemi-Lumi One (Nacalai Tesque).

Article Title: Separate Cellular Localizations of Human T-Lymphotropic Virus 1 (HTLV-1) Env and Glucose Transporter Type 1 (GLUT1) Are Required for HTLV-1 Env-Mediated Fusion and Infection
Article Snippet: The cell lysates or VLPs then were separated by SDS-PAGE and blotted onto PVDF (polyvinylidene difluoride; Immobilon-P; Millipore, Billerica, MA, USA) membranes. .. The signals were detected using Chemi-Lumi One (Nacalai Tesque).

Article Title: Rapid labelling and covalent inhibition of intracellular native proteins using ligand-directed N-acyl-N-alkyl sulfonamide
Article Snippet: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting were carried out with a Bio-Rad Mini-Protean III electrophoresis apparatus. .. Fluorescence gel images and chemical luminescent signals using Chemi-Lumi one (Nacalai Tesque) or ECL Prime (GE Healthcare) were obtained by an imagequant LAS4000 (Fujifilm).

Article Title: Learning and Memory Deficits in Male Adult Mice Treated with a Benzodiazepine Sleep-Inducing Drug during the Juvenile Period
Article Snippet: The protein samples (30 μg/well) were subjected to SDS-PAGE (7.5% polyacrylamide), and transferred to a nitrocellulose membrane. .. After several PBS-T washes, the membranes were incubated with chemi-lumi one L (Nacalai Tesque, Inc., Kyoto, Japan) for signal production.

Article Title: Chemical labelling for visualizing native AMPA receptors in live neurons
Article Snippet: The samples were applied to SDS-PAGE and electrotransferred onto immune-blot polyvinylidene fluoride (PVDF) membranes (Bio-Rad), followed by blocking with 5% nonfat dry milk in Tris-buffered saline (TBS) containing 0.05% Tween (Sigma-Aldrich). .. The signals were developed with Chemi-Lumi One (Nacalai tesque) or ECL Prime Western Blotting Detection Reagent (GE Healthcare) and detected with Imagequant LAS4000 (GE Healthcare).

Article Title: Chemical labelling for visualizing native AMPA receptors in live neurons
Article Snippet: SDS–PAGE and western blotting were carried out using a Bio-Rad Mini-Protean III electrophoresis apparatus. .. Chemiluminescent signals generated with Chemi-Lumi One (nacalai tesque) or ECL Prime (GE Healthcare) were detected with an LAS4000 imaging system (Fuji Film).

Article Title: Cellular and viral chromatin proteins are positive factors in the regulation of adenovirus gene expression
Article Snippet: Cell lysates or nuclear/cytoplasmic fractions were subjected to SDS–PAGE, and then proteins were transferred to a PVDF membrane (Millipore). .. The blots were developed using Chemi-Lumi One (Nacalai tesque).

Article Title: Metformin treatment ameliorates diabetes‐associated decline in hippocampal neurogenesis and memory via phosphorylation of insulin receptor substrate 1
Article Snippet: A total of 15 μg of each SDS protein sample was loaded per lane, separated by 7.5% SDS/PAGE, and transferred to nitrocellulose membranes. .. The chemiluminescent signals were detected using Chemi‐Lumi One (Nacalai Tesque) or ImmunoStar LD (Wako).

Software:

Article Title: A Synthetic Analogue of Neopeltolide, 8,9-Dehydroneopeltolide, Is a Potent Anti-Austerity Agent against Starved Tumor Cells
Article Snippet: The membrane was rinsed with T-TBS (×3) and probed with an appropriate HRP-conjugated secondary antibody at room temperature for 1 h. The membrane was rinsed with T-TBS (5 min × 3) and developed with Chemi-Lumi One (Nacalai Tesque, Kyoto, Japan). .. Chemiluminescence was detected on a ChemiDoc XRS + Imaging System (Bio-Rad, Hercules, CA, USA).

Electrophoresis:

Article Title: Rapid labelling and covalent inhibition of intracellular native proteins using ligand-directed N-acyl-N-alkyl sulfonamide
Article Snippet: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting were carried out with a Bio-Rad Mini-Protean III electrophoresis apparatus. .. Fluorescence gel images and chemical luminescent signals using Chemi-Lumi one (Nacalai Tesque) or ECL Prime (GE Healthcare) were obtained by an imagequant LAS4000 (Fujifilm).

Article Title: Chemical labelling for visualizing native AMPA receptors in live neurons
Article Snippet: SDS–PAGE and western blotting were carried out using a Bio-Rad Mini-Protean III electrophoresis apparatus. .. Chemiluminescent signals generated with Chemi-Lumi One (nacalai tesque) or ECL Prime (GE Healthcare) were detected with an LAS4000 imaging system (Fuji Film).

RNA Extraction:

Article Title: Induction of Apoptosis in MCF-7 Cells via Oxidative Stress Generation, Mitochondria-Dependent and Caspase-Independent Pathway by Ethyl Acetate Extract of Dillenia suffruticosa and Its Chemical Profile
Article Snippet: Real Genomics Total RNA Extraction Kit (RBC Biosciences, Taiwan) and GenomeLab GeXP Start Kit (Beckman Coulter, USA) were also purchased. .. Phosphatase inhibitor cocktails, bovine serum albumin (BSA) and Chemi-Lumi One L were purchased from Nacalai Tesque (Kyoto, Japan).

Binding Assay:

Article Title: Human LYPD8 protein inhibits motility of flagellated bacteria
Article Snippet: Paragraph title: Pull-down assay for LYPD8 binding to flagella ... Immunoreactivity was detected using Chemi-Lumi One (Nacalai Tesque).

Article Title: Hypoxia‐inducible factor‐targeting prodrug TOP3 combined with gemcitabine or TS‐1 improves pancreatic cancer survival in an orthotopic model
Article Snippet: Actin, HIF‐1α, and HIF‐2α on the filter were probed with the following antibodies: mouse anti‐actin (A4700; Sigma‐Aldrich), rabbit anti‐HIF‐1α (NB100‐134) and rabbit anti‐HIF‐2α (NB100‐122) (Novus Biologicals, Littleton, CO, USA), anti‐mouse IgG conjugated with HRP (#7076) and anti‐rabbit IgG conjugated with HRP (#7074) (Cell Signaling Technology, Danvers, MA, USA). .. Binding was detected with Chemi‐Lumi One Ultra, a chemiluminescence detection reagent (Nacalai Tesque). .. Cells were seeded at a density of 2 × 103 cells/well in 96‐well plates and pre‐incubated for 12 h in normoxia.

Radio Immunoprecipitation:

Article Title: Chemical labelling for visualizing native AMPA receptors in live neurons
Article Snippet: Fluorescence images were acquired using a 488 nm line of an argon laser for excitation of OG and a HeNe Green laser for excitation of mCherry-F. For western blot analysis, after chemical labelling, cells were washed 3 times with ice-cold HBS, lysed with radio immunoprecipitation assay (RIPA) buffer containing 1% protease inhibitor cocktail set III (Calbiochem), and mixed with a quarter volume of 5 × SDS–PAGE loading buffer containing 250 mM DTT. .. The signals were developed with Chemi-Lumi One (Nacalai tesque) or ECL Prime Western Blotting Detection Reagent (GE Healthcare) and detected with Imagequant LAS4000 (GE Healthcare).

Homogenization:

Article Title: Cyclocarya paliurus extract activates insulin signaling via Sirtuin1 in C2C12 myotubes and decreases blood glucose level in mice with impaired insulin secretion
Article Snippet: The protein was extracted via homogenization in an ice-cold RIPA buffer. .. Finally, detection was achieved using Chemi-Lumi one super (Nacalai Tesque, Kyoto).

Protein Binding:

Article Title: Chronic endometritis modifies decidualization in human endometrial stromal cells
Article Snippet: Membranes were incubated with 20% blocking reagent-N102 (NOF Co.) in 25 mM TBS containing 0.1% Tween-20 (TBST; pH 7.4) at room temperature for 1 h to block non-specific protein binding sites, followed by incubation with the IGFBP-1 antibody (1: 1000; Santa Cruz Biotechnology, Inc.) or GAPDH (1:5000; Enzo Life Science, Inc.) diluted by TBST at 4 °C overnight. .. Peroxidase labeling was detected by chemiluminescence using the Chemi-Lumi one super (Nacalai Tesque).

Immunodetection:

Article Title: Chemical labelling for visualizing native AMPA receptors in live neurons
Article Snippet: The immunodetection of GluA2 was performed with a rabbit anti-GluA2/3 antibody (Millipore, 07-598, × 3,000) and anti-rabbit IgG-HRP conjugate (Santa Cruz, sc-2004 × 3,000). .. The signals were developed with Chemi-Lumi One (Nacalai tesque) or ECL Prime Western Blotting Detection Reagent (GE Healthcare) and detected with Imagequant LAS4000 (GE Healthcare).

Spectrophotometry:

Article Title: Rapid labelling and covalent inhibition of intracellular native proteins using ligand-directed N-acyl-N-alkyl sulfonamide
Article Snippet: Fluorescence gel images and chemical luminescent signals using Chemi-Lumi one (Nacalai Tesque) or ECL Prime (GE Healthcare) were obtained by an imagequant LAS4000 (Fujifilm). .. Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) spectra were measured with an Autoflex III, Ultraflex III or UltrafleXtreme (Bruker Daltonics) using α-cyano-4-hydroxycinnamic acid (CHCA) or sinapic acid as the matrix.

Immunoprecipitation:

Article Title: F-fucoidan from Saccharina japonica is a novel inducer of galectin-9 and exhibits anti-allergic activity
Article Snippet: Paragraph title: Immunoprecipitation, SDS-PAGE and western blotting ... Anti-mouse galectin-9 or anti-mouse IgE antibodies were reacted as the primary antibody at 4°C overnight, followed by anti-goat IgG-HRP antibody as the secondary antibody at 4°C for 1 h. All signals were detected by enhanced chemiluminescence using Chemi-Lumi One (Nacalai Tesque).

Article Title: Separate Cellular Localizations of Human T-Lymphotropic Virus 1 (HTLV-1) Env and Glucose Transporter Type 1 (GLUT1) Are Required for HTLV-1 Env-Mediated Fusion and Infection
Article Snippet: Paragraph title: Immunoprecipitation and Western blotting. ... The signals were detected using Chemi-Lumi One (Nacalai Tesque).

Staining:

Article Title: Methylation and Acetylation Enhanced the Antidiabetic Activity of Some Selected Flavonoids: In Vitro, Molecular Modelling and Structure Activity Relationship-Based Study
Article Snippet: For Western blotting, 10% SDS TGX stain free™ FastCast acrylamide gel kits, Immun-Blot® polyvinylidene difluoride (PVDF), non-fat dried milk were purchased from Bio-Rad, Santa Rosa, CA, USA. .. Chemiluminescence, Chemi-Lumi One L and Tween-20 were bought from Nacalai-tesque, Kyoto, Japan.

Article Title: Separate Cellular Localizations of Human T-Lymphotropic Virus 1 (HTLV-1) Env and Glucose Transporter Type 1 (GLUT1) Are Required for HTLV-1 Env-Mediated Fusion and Infection
Article Snippet: The membranes were incubated with anti-gp46 rat MAb (LAT-27), anti-FLAG mouse MAb (Wako, Osaka, Japan), anti-HA mouse MAb (Wako), anti-HIV-1 Gag mouse MAb (24-3) , anti-cyclophilin A (CypA) rabbit polyclonal antibody (Enzo Life Sciences, Farmingdale, NY, USA), or anti-β-actin mouse MAb (Sigma), followed by staining with horseradish peroxidase (HRP)-conjugated anti-mouse (Jackson ImmunoResearch, West Grove, PA, USA), anti-rat (Jackson ImmunoResearch), or anti-rabbit (Jackson ImmunoResearch) IgG. .. The signals were detected using Chemi-Lumi One (Nacalai Tesque).

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    Nacalai chemi lumi one l western blotting detection reagents
    Chemi Lumi One L Western Blotting Detection Reagents, supplied by Nacalai, used in various techniques. Bioz Stars score: 91/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nacalai chemi lumi one hrp chemiluminescence substrate
    Chemi Lumi One Hrp Chemiluminescence Substrate, supplied by Nacalai, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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