Structured Review

Nacalai chemi lumi
Chemi Lumi, supplied by Nacalai, used in various techniques. Bioz Stars score: 91/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chemi lumi/product/Nacalai
Average 91 stars, based on 82 article reviews
Price from $9.99 to $1999.99
chemi lumi - by Bioz Stars, 2020-07
91/100 stars

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Related Articles

Modification:

Article Title: Copper diethyldithiocarbamate as an activator of Nrf2 in cultured vascular endothelial cells
Article Snippet: .. The following materials were purchased from the respective vendors: Dulbecco’s modified Eagle’s medium and calcium- and magnesium-free phosphate buffered saline from Nissui Pharmaceutical (Tokyo, Japan); fetal bovine serum from HyClone Laboratories (Waltham, MA, USA); biotin-PEAC5 -maleimide (BPM) from Dojindo (Kumamoto, Japan); rabbit polyclonal anti-actin antibody (A5060) and copper(II) diacetate (Cu04) from Sigma Aldrich Chemical (St. Louis, MO, USA); horseradish peroxidase-conjugated anti-rabbit IgG antibody (#7074) and anti-biotin, horseradish peroxidase-linked antibody (#7075) from Cell Signaling (Beverly, MA, USA); anti-NAD(P)H quinone oxidoreductase 1 antibody (ab2346) and donkey polyclonal antibody to goat IgG-horseradish peroxidase (ab6885) from Abcam (Tokyo, Japan); rabbit polyclonal anti Nrf2 antibody (H-300), rabbit polyclonal anti CTR1 antibody (FL-190), mouse polyclonal anti Keap1 antibody (H-190), and rabbit polyclonal anti γ-glutamylcysteine synthetase modifier subunit antibody (FL-274) from Santa Cruz Biotechnology (Santa Cruz, CA, USA); rabbit polyclonal anti-heme oxygenase-1 antibody (ADI-SPA-895) and ubiquitin monoclonal antibody (ADI-SPA-203), and MG-132 from Enzo Life Sciences (Farmingdale, NY, USA); nitric oxide, hydrogen peroxide, copper(II) bis(dimethyldithiocarbamate) (Cu17), and sodium diethyldithiocarbamate trihydrate (Na01) from Wako Pure Chemical Industries (Osaka, Japan); 3,5-diaminobenzoic acid, bis(hexafluoroactylacetonato)copper(II) (Cu01), bis(1,3-propanediamine)copper(II) dichloride (Cu07), copper(II) bis(2-hydroxyethyl)dithiocarbamate (Cu09), copper(II) bis(diethyldithiocarbamate) (Cu10), iron(II) phthalocyanine (Fe03), sodium iron(III) ethylenediaminetetraacetate (Fe04), iron(III) tris(diethyldithiocarbamate) (Fe05), nickel(II) bis(diethyldithiocarbamate) (Ni06), zinc(II) bis(diethyldithiocarbamate) (Zn01), zinc(II) bis(dibutyldithiocarbamate), and zinc(II) bis(dibenzyldithiocarbamate) from Tokyo Chemical Industry (Tokyo, Japan); iron(II) diacetate (Fe02) from Acros Organics (Thermo Fisher Scientific, Geel, Belgium); Chemi-Lumi One L and other reagents were from Nacalai Tesque (Kyoto, Japan). ..

Western Blot:

Article Title: Differential lactate and cholesterol synthetic activities in XY and XX Sertoli cells
Article Snippet: .. Bound antibodies were detected using the Chemi-Lumi One L Western Blotting Detection System (Nacalai Tesque, Kyoto, Japan). .. Chromatin immunoprecipitation-sequence (ChIP-seq) A total of 106 Sertoli cells fixed by formaldehyde (0.5%, 5 min at room temperature) were lysed with 600 μl Lysis Buffer (5 mM HEPES (pH 8.0), 200 mM KCl, 1 mM CaCl2 , 1.5 mM MgCl2 , 5% Sucrose, 0.5% Triton X-100), and then sonicated (5 × 15-s pulses with 59-s break intervals) using the Bioruptor plus sonication device (Diagenode, Denville, NJ, USA).

Article Title: Induction of cell cycle arrest and apoptosis in caspase-3 deficient MCF-7 cells by Dillenia suffruticosa root extract via multiple signalling pathways
Article Snippet: .. Western blot analysis Bovine serum albumen (BSA), phosphatase inhibitor cocktails and Chemi-Lumi One L were purchased from Nacalai Tesque (Kyoto, Japan). .. Phenylmethanesulfonyl fluoride (PMSF) and protease inhibitor cocktails were purchased from Calbiochem (San Diego, CA, USA).

Incubation:

Article Title: Crosslinking of a Peritrophic Matrix Protein Protects Gut Epithelia from Bacterial Exotoxins
Article Snippet: .. After blocking with 5% dry milk, the membrane was incubated at room temperature for 1 h with the anti-drosocrystallin antibody and then with the secondary antibody (horseradish peroxidase-conjugated goat anti-rabbit IgG; Bio-Rad Laboratories), followed by development with Chemi-Lumi One, Chemi-Lumi One-super (Nacalai), or WesternBright Sirius (Advansta). .. For detection of the His-tag, horseradish peroxidase-conjugated anti-6 × His tag antibody (MBL International) was used.

other:

Article Title: Chemical labelling for visualizing native AMPA receptors in live neurons
Article Snippet: The signals were developed with Chemi-Lumi One or ECL-Prime and detected with LAS4000.

Blocking Assay:

Article Title: Crosslinking of a Peritrophic Matrix Protein Protects Gut Epithelia from Bacterial Exotoxins
Article Snippet: .. After blocking with 5% dry milk, the membrane was incubated at room temperature for 1 h with the anti-drosocrystallin antibody and then with the secondary antibody (horseradish peroxidase-conjugated goat anti-rabbit IgG; Bio-Rad Laboratories), followed by development with Chemi-Lumi One, Chemi-Lumi One-super (Nacalai), or WesternBright Sirius (Advansta). .. For detection of the His-tag, horseradish peroxidase-conjugated anti-6 × His tag antibody (MBL International) was used.

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  • 91
    Nacalai chemi lumi
    Chemi Lumi, supplied by Nacalai, used in various techniques. Bioz Stars score: 91/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chemi lumi/product/Nacalai
    Average 91 stars, based on 82 article reviews
    Price from $9.99 to $1999.99
    chemi lumi - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    92
    Nacalai chemi lumi one super kit
    Chemi Lumi One Super Kit, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chemi lumi one super kit/product/Nacalai
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    chemi lumi one super kit - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Nacalai chemi lumi one super solution
    Chemi Lumi One Super Solution, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chemi lumi one super solution/product/Nacalai
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    chemi lumi one super solution - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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