chemi lumi one l western blotting detection reagents  (Nacalai)

 
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    Nacalai chemi lumi one l western blotting detection reagents
    Chemi Lumi One L Western Blotting Detection Reagents, supplied by Nacalai, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chemi lumi one l western blotting detection reagents/product/Nacalai
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chemi lumi one l western blotting detection reagents - by Bioz Stars, 2020-02
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: Interaction between a Domain of the Negative Regulator of the Ras-ERK Pathway, SPRED1 Protein, and the GTPase-activating Protein-related Domain of Neurofibromin Is Implicated in Legius Syndrome and Neurofibromatosis Type 1 *
    Article Snippet: Paragraph title: Cell Culture and Transfection ... The membranes were blotted with the indicated antibodies, and the bound antibodies were visualized using horseradish peroxidase-conjugated antibodies against goat, rabbit, or mouse IgG, and Chemi-Lumi One L Western blotting detection reagents (Nacalai Tesque).

    Mutagenesis:

    Article Title: TRAF6 and MEKK1 Play a Pivotal Role in the RIG-I-like Helicase Antiviral Pathway *
    Article Snippet: The T7-tagged MEKK3 expression vector T7-MEKK3, T7-Lys(391)-Met MEKK3 mutant (kn-MEKK3), and T7-Lys(432)-Met MEKK1 mutant (kn-MEKK1) were generated by PCR ( ). .. The membranes were immunoblotted with various antibodies, and the bound antibodies were visualized with horseradish peroxidase-conjugated antibodies against rabbit or mouse IgG (Jackson ImmunoResearch Laboratories), using Chemi-Lumi One L Western blotting detection reagents (Nacalai Tesque).

    Isolation:

    Article Title: Structural and functional insights into S-thiolation of human serum albumins
    Article Snippet: Immunoblot analysis of serum protein redHSA and oxHSA isolated from human sera were boiled with Laemmli sample buffer for 10 min at 80 °C. .. This procedure was followed by the addition of Chemi-Lumi One L western blotting detection reagents (Nacalai Tesque, Tokyo, Japan).

    Western Blot:

    Article Title: Structural and functional insights into S-thiolation of human serum albumins
    Article Snippet: .. This procedure was followed by the addition of Chemi-Lumi One L western blotting detection reagents (Nacalai Tesque, Tokyo, Japan). ..

    Article Title: TRAF6 and MEKK1 Play a Pivotal Role in the RIG-I-like Helicase Antiviral Pathway *
    Article Snippet: .. The membranes were immunoblotted with various antibodies, and the bound antibodies were visualized with horseradish peroxidase-conjugated antibodies against rabbit or mouse IgG (Jackson ImmunoResearch Laboratories), using Chemi-Lumi One L Western blotting detection reagents (Nacalai Tesque). .. For native PAGE assay, MEFs were seeded at 4 × 105 cells/well in 6-well plates.

    Article Title: Interaction between a Domain of the Negative Regulator of the Ras-ERK Pathway, SPRED1 Protein, and the GTPase-activating Protein-related Domain of Neurofibromin Is Implicated in Legius Syndrome and Neurofibromatosis Type 1 *
    Article Snippet: .. The membranes were blotted with the indicated antibodies, and the bound antibodies were visualized using horseradish peroxidase-conjugated antibodies against goat, rabbit, or mouse IgG, and Chemi-Lumi One L Western blotting detection reagents (Nacalai Tesque). .. The Elk-1 activation was measured by the GAL4 DNA-binding domain/Elk-1 fusion system according to the manufacturer's instructions (PathDetect in vivo signal transduction pathway trans-reporting system, Agilent Technologies) as described ( ).

    Centrifugation:

    Article Title: Interaction between a Domain of the Negative Regulator of the Ras-ERK Pathway, SPRED1 Protein, and the GTPase-activating Protein-related Domain of Neurofibromin Is Implicated in Legius Syndrome and Neurofibromatosis Type 1 *
    Article Snippet: After lysis, cellular debris was removed by centrifugation at 15,000 × g for 10 min. .. The membranes were blotted with the indicated antibodies, and the bound antibodies were visualized using horseradish peroxidase-conjugated antibodies against goat, rabbit, or mouse IgG, and Chemi-Lumi One L Western blotting detection reagents (Nacalai Tesque).

    Blocking Assay:

    Article Title: Structural and functional insights into S-thiolation of human serum albumins
    Article Snippet: After electrophoresis, the gel was stained with Coomassie Brilliant Blue or transblotted onto a PVDF membrane and incubated with 0.25% polyvinylpyrrolidone (Sigma, St. Louis, MO, USA) in TBS/T (Tris-buffered saline containing 10% Tween 20) for blocking, washed, and treated with the primary antibodies at 4 °C. .. This procedure was followed by the addition of Chemi-Lumi One L western blotting detection reagents (Nacalai Tesque, Tokyo, Japan).

    Electrophoresis:

    Article Title: Structural and functional insights into S-thiolation of human serum albumins
    Article Snippet: After electrophoresis, the gel was stained with Coomassie Brilliant Blue or transblotted onto a PVDF membrane and incubated with 0.25% polyvinylpyrrolidone (Sigma, St. Louis, MO, USA) in TBS/T (Tris-buffered saline containing 10% Tween 20) for blocking, washed, and treated with the primary antibodies at 4 °C. .. This procedure was followed by the addition of Chemi-Lumi One L western blotting detection reagents (Nacalai Tesque, Tokyo, Japan).

    Immunoprecipitation:

    Article Title: Interaction between a Domain of the Negative Regulator of the Ras-ERK Pathway, SPRED1 Protein, and the GTPase-activating Protein-related Domain of Neurofibromin Is Implicated in Legius Syndrome and Neurofibromatosis Type 1 *
    Article Snippet: Protein from cell lysates was precipitated using 2 μg of antibody and 25 μl of TrueBlot® anti-mouse Ig immunoprecipitation beads or protein G-Sepharose (GE Healthcare) for 2 h at 4 °C. .. The membranes were blotted with the indicated antibodies, and the bound antibodies were visualized using horseradish peroxidase-conjugated antibodies against goat, rabbit, or mouse IgG, and Chemi-Lumi One L Western blotting detection reagents (Nacalai Tesque).

    Incubation:

    Article Title: Structural and functional insights into S-thiolation of human serum albumins
    Article Snippet: After washing with TBS/T, the blots were further incubated for 1 h at room temperature with the HRP-linked secondary anti-IgG antibody. .. This procedure was followed by the addition of Chemi-Lumi One L western blotting detection reagents (Nacalai Tesque, Tokyo, Japan).

    Cell Culture:

    Article Title: Interaction between a Domain of the Negative Regulator of the Ras-ERK Pathway, SPRED1 Protein, and the GTPase-activating Protein-related Domain of Neurofibromin Is Implicated in Legius Syndrome and Neurofibromatosis Type 1 *
    Article Snippet: Paragraph title: Cell Culture and Transfection ... The membranes were blotted with the indicated antibodies, and the bound antibodies were visualized using horseradish peroxidase-conjugated antibodies against goat, rabbit, or mouse IgG, and Chemi-Lumi One L Western blotting detection reagents (Nacalai Tesque).

    Expressing:

    Article Title: TRAF6 and MEKK1 Play a Pivotal Role in the RIG-I-like Helicase Antiviral Pathway *
    Article Snippet: The expression vectors for FLAG-TRAF, HA-TAK1, HA-TAB-1, FLAG-IRF3, and NF-κB-Luc have been described previously ( , ). .. The membranes were immunoblotted with various antibodies, and the bound antibodies were visualized with horseradish peroxidase-conjugated antibodies against rabbit or mouse IgG (Jackson ImmunoResearch Laboratories), using Chemi-Lumi One L Western blotting detection reagents (Nacalai Tesque).

    Protease Inhibitor:

    Article Title: TRAF6 and MEKK1 Play a Pivotal Role in the RIG-I-like Helicase Antiviral Pathway *
    Article Snippet: Western Blotting —Cells were lysed in 0.5% Triton X-100 extraction buffer containing 20 m m HEPES (pH 7.4), 150 m m NaCl, 1.5 m m MgCl2 , 2 m m dithiothreitol supplemented with phosphatase and protease inhibitor mixture (Nacalai Tesque). .. The membranes were immunoblotted with various antibodies, and the bound antibodies were visualized with horseradish peroxidase-conjugated antibodies against rabbit or mouse IgG (Jackson ImmunoResearch Laboratories), using Chemi-Lumi One L Western blotting detection reagents (Nacalai Tesque).

    Article Title: Interaction between a Domain of the Negative Regulator of the Ras-ERK Pathway, SPRED1 Protein, and the GTPase-activating Protein-related Domain of Neurofibromin Is Implicated in Legius Syndrome and Neurofibromatosis Type 1 *
    Article Snippet: Cells were lysed in 0.5 ml of TNE lysis buffer containing 150 m m Tris-HCl (pH 7.6), 50 m m NaCl, 1 m m EDTA, and 0.5% Nonidet P-40 supplemented with protease inhibitor mixture (Nacalai Tesque) ( ). .. The membranes were blotted with the indicated antibodies, and the bound antibodies were visualized using horseradish peroxidase-conjugated antibodies against goat, rabbit, or mouse IgG, and Chemi-Lumi One L Western blotting detection reagents (Nacalai Tesque).

    Polymerase Chain Reaction:

    Article Title: TRAF6 and MEKK1 Play a Pivotal Role in the RIG-I-like Helicase Antiviral Pathway *
    Article Snippet: The T7-tagged MEKK3 expression vector T7-MEKK3, T7-Lys(391)-Met MEKK3 mutant (kn-MEKK3), and T7-Lys(432)-Met MEKK1 mutant (kn-MEKK1) were generated by PCR ( ). .. The membranes were immunoblotted with various antibodies, and the bound antibodies were visualized with horseradish peroxidase-conjugated antibodies against rabbit or mouse IgG (Jackson ImmunoResearch Laboratories), using Chemi-Lumi One L Western blotting detection reagents (Nacalai Tesque).

    Staining:

    Article Title: Structural and functional insights into S-thiolation of human serum albumins
    Article Snippet: After electrophoresis, the gel was stained with Coomassie Brilliant Blue or transblotted onto a PVDF membrane and incubated with 0.25% polyvinylpyrrolidone (Sigma, St. Louis, MO, USA) in TBS/T (Tris-buffered saline containing 10% Tween 20) for blocking, washed, and treated with the primary antibodies at 4 °C. .. This procedure was followed by the addition of Chemi-Lumi One L western blotting detection reagents (Nacalai Tesque, Tokyo, Japan).

    Lysis:

    Article Title: Interaction between a Domain of the Negative Regulator of the Ras-ERK Pathway, SPRED1 Protein, and the GTPase-activating Protein-related Domain of Neurofibromin Is Implicated in Legius Syndrome and Neurofibromatosis Type 1 *
    Article Snippet: After lysis, cellular debris was removed by centrifugation at 15,000 × g for 10 min. .. The membranes were blotted with the indicated antibodies, and the bound antibodies were visualized using horseradish peroxidase-conjugated antibodies against goat, rabbit, or mouse IgG, and Chemi-Lumi One L Western blotting detection reagents (Nacalai Tesque).

    Clear Native PAGE:

    Article Title: Structural and functional insights into S-thiolation of human serum albumins
    Article Snippet: The samples were electrophoresed through a reduced and non-reduced SDS-PAGE (10% polyacrylamide gel) and native-PAGE gel. .. This procedure was followed by the addition of Chemi-Lumi One L western blotting detection reagents (Nacalai Tesque, Tokyo, Japan).

    Article Title: TRAF6 and MEKK1 Play a Pivotal Role in the RIG-I-like Helicase Antiviral Pathway *
    Article Snippet: The membranes were immunoblotted with various antibodies, and the bound antibodies were visualized with horseradish peroxidase-conjugated antibodies against rabbit or mouse IgG (Jackson ImmunoResearch Laboratories), using Chemi-Lumi One L Western blotting detection reagents (Nacalai Tesque). .. For native PAGE assay, MEFs were seeded at 4 × 105 cells/well in 6-well plates.

    SDS Page:

    Article Title: Structural and functional insights into S-thiolation of human serum albumins
    Article Snippet: The samples were electrophoresed through a reduced and non-reduced SDS-PAGE (10% polyacrylamide gel) and native-PAGE gel. .. This procedure was followed by the addition of Chemi-Lumi One L western blotting detection reagents (Nacalai Tesque, Tokyo, Japan).

    Article Title: TRAF6 and MEKK1 Play a Pivotal Role in the RIG-I-like Helicase Antiviral Pathway *
    Article Snippet: The cell lysates were resolved by SDS-PAGE and transferred to Immobilon-P membranes (Millipore). .. The membranes were immunoblotted with various antibodies, and the bound antibodies were visualized with horseradish peroxidase-conjugated antibodies against rabbit or mouse IgG (Jackson ImmunoResearch Laboratories), using Chemi-Lumi One L Western blotting detection reagents (Nacalai Tesque).

    Article Title: Interaction between a Domain of the Negative Regulator of the Ras-ERK Pathway, SPRED1 Protein, and the GTPase-activating Protein-related Domain of Neurofibromin Is Implicated in Legius Syndrome and Neurofibromatosis Type 1 *
    Article Snippet: For Western blotting, the immunoprecipitates or whole cell lysates were resolved using SDS-PAGE and transferred to Immobilon-P membranes (Millipore). .. The membranes were blotted with the indicated antibodies, and the bound antibodies were visualized using horseradish peroxidase-conjugated antibodies against goat, rabbit, or mouse IgG, and Chemi-Lumi One L Western blotting detection reagents (Nacalai Tesque).

    Plasmid Preparation:

    Article Title: TRAF6 and MEKK1 Play a Pivotal Role in the RIG-I-like Helicase Antiviral Pathway *
    Article Snippet: The T7-tagged MEKK3 expression vector T7-MEKK3, T7-Lys(391)-Met MEKK3 mutant (kn-MEKK3), and T7-Lys(432)-Met MEKK1 mutant (kn-MEKK1) were generated by PCR ( ). .. The membranes were immunoblotted with various antibodies, and the bound antibodies were visualized with horseradish peroxidase-conjugated antibodies against rabbit or mouse IgG (Jackson ImmunoResearch Laboratories), using Chemi-Lumi One L Western blotting detection reagents (Nacalai Tesque).

    Generated:

    Article Title: TRAF6 and MEKK1 Play a Pivotal Role in the RIG-I-like Helicase Antiviral Pathway *
    Article Snippet: The T7-tagged MEKK3 expression vector T7-MEKK3, T7-Lys(391)-Met MEKK3 mutant (kn-MEKK3), and T7-Lys(432)-Met MEKK1 mutant (kn-MEKK1) were generated by PCR ( ). .. The membranes were immunoblotted with various antibodies, and the bound antibodies were visualized with horseradish peroxidase-conjugated antibodies against rabbit or mouse IgG (Jackson ImmunoResearch Laboratories), using Chemi-Lumi One L Western blotting detection reagents (Nacalai Tesque).

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  • News
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  • Team
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  • Partners
  • Contact
  • Bioz Stars
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  • 99
    Nacalai chemi lumi one l western blotting detection reagents
    Chemi Lumi One L Western Blotting Detection Reagents, supplied by Nacalai, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chemi lumi one l western blotting detection reagents/product/Nacalai
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chemi lumi one l western blotting detection reagents - by Bioz Stars, 2020-02
    99/100 stars
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