chemi lumi one hrp chemiluminescence substrate  (Nacalai)

 
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    Structured Review

    Nacalai chemi lumi one hrp chemiluminescence substrate
    Chemi Lumi One Hrp Chemiluminescence Substrate, supplied by Nacalai, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chemi lumi one hrp chemiluminescence substrate/product/Nacalai
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chemi lumi one hrp chemiluminescence substrate - by Bioz Stars, 2020-02
    89/100 stars

    Related Products / Commonly Used Together

    tbst
    antibody incubation
    horseradish peroxidase hrp -

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    Related Articles

    Incubation:

    Article Title: Differences in Gene Expression Profiles and Phenotypes of Differentiated SH-SY5Y Neurons Stably Overexpressing Mitochondrial Ferritin
    Article Snippet: .. After antibody incubation, membranes were sequentially washed 3 × 10 min in TBST, and incubated in the appropriate horseradish peroxidase (HRP)-conjugated anti-immunoglobulin for 2 h. Membranes were imaged using Chemi-Lumi-One HRP chemiluminescence substrate (Nacalai-Tesque) with a LAS4000 Imaging system (GE Biosciences, U.S.A.). .. Some membranes were reprobed with different antibodies after treatment with Stripping Agent-Strong (Nacalai-Tesque).

    Stripping Membranes:

    Article Title: Differences in Gene Expression Profiles and Phenotypes of Differentiated SH-SY5Y Neurons Stably Overexpressing Mitochondrial Ferritin
    Article Snippet: After antibody incubation, membranes were sequentially washed 3 × 10 min in TBST, and incubated in the appropriate horseradish peroxidase (HRP)-conjugated anti-immunoglobulin for 2 h. Membranes were imaged using Chemi-Lumi-One HRP chemiluminescence substrate (Nacalai-Tesque) with a LAS4000 Imaging system (GE Biosciences, U.S.A.). .. Some membranes were reprobed with different antibodies after treatment with Stripping Agent-Strong (Nacalai-Tesque).

    Imaging:

    Article Title: Differences in Gene Expression Profiles and Phenotypes of Differentiated SH-SY5Y Neurons Stably Overexpressing Mitochondrial Ferritin
    Article Snippet: .. After antibody incubation, membranes were sequentially washed 3 × 10 min in TBST, and incubated in the appropriate horseradish peroxidase (HRP)-conjugated anti-immunoglobulin for 2 h. Membranes were imaged using Chemi-Lumi-One HRP chemiluminescence substrate (Nacalai-Tesque) with a LAS4000 Imaging system (GE Biosciences, U.S.A.). .. Some membranes were reprobed with different antibodies after treatment with Stripping Agent-Strong (Nacalai-Tesque).

    Expressing:

    Article Title: Differences in Gene Expression Profiles and Phenotypes of Differentiated SH-SY5Y Neurons Stably Overexpressing Mitochondrial Ferritin
    Article Snippet: After antibody incubation, membranes were sequentially washed 3 × 10 min in TBST, and incubated in the appropriate horseradish peroxidase (HRP)-conjugated anti-immunoglobulin for 2 h. Membranes were imaged using Chemi-Lumi-One HRP chemiluminescence substrate (Nacalai-Tesque) with a LAS4000 Imaging system (GE Biosciences, U.S.A.). .. Results are calculated as relative expression after normalization for levels of β-actin.

    Western Blot:

    Article Title: Differences in Gene Expression Profiles and Phenotypes of Differentiated SH-SY5Y Neurons Stably Overexpressing Mitochondrial Ferritin
    Article Snippet: Paragraph title: Western Blot Detection and Quantification ... After antibody incubation, membranes were sequentially washed 3 × 10 min in TBST, and incubated in the appropriate horseradish peroxidase (HRP)-conjugated anti-immunoglobulin for 2 h. Membranes were imaged using Chemi-Lumi-One HRP chemiluminescence substrate (Nacalai-Tesque) with a LAS4000 Imaging system (GE Biosciences, U.S.A.).

    Software:

    Article Title: Differences in Gene Expression Profiles and Phenotypes of Differentiated SH-SY5Y Neurons Stably Overexpressing Mitochondrial Ferritin
    Article Snippet: After antibody incubation, membranes were sequentially washed 3 × 10 min in TBST, and incubated in the appropriate horseradish peroxidase (HRP)-conjugated anti-immunoglobulin for 2 h. Membranes were imaged using Chemi-Lumi-One HRP chemiluminescence substrate (Nacalai-Tesque) with a LAS4000 Imaging system (GE Biosciences, U.S.A.). .. For normalization and loading control, membranes were subsequently incubated with an HRP-conjugated antibody to β-actin (FujiFilm/Wako, Japan) at 1:15,000 for 1 h. All images were quantified using Image Studio Lite software (Licor, NE, USA).

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  • News
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  • Bioz Stars
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  • 89
    Nacalai chemi lumi one hrp chemiluminescence substrate
    Chemi Lumi One Hrp Chemiluminescence Substrate, supplied by Nacalai, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chemi lumi one hrp chemiluminescence substrate/product/Nacalai
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chemi lumi one hrp chemiluminescence substrate - by Bioz Stars, 2020-02
    89/100 stars
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