chargeswitch pcr clean up  (Thermo Fisher)


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    Name:
    ChargeSwitch PCR Clean Up Kit
    Description:
    • Sample size 25 50 µl• High recovery of DNA fragments• Rapid one tube 5 minute protocolChargeSwitch PCR Clean Up Kit is the fastest and most effective method of removing primers unincorporated dNTPs thermostable polymerases and salts Figure 1 This kit is scalable for use on liquid handling robots This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory Please see the Accessories Chapter Chapter 6 to select the best magnetic accessory for your needs
    Catalog Number:
    cs12000
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|DNA Extraction|PCR Product Clean-Up
    Category:
    Kits and Assays
    Buy from Supplier


    Structured Review

    Thermo Fisher chargeswitch pcr clean up
    • Sample size 25 50 µl• High recovery of DNA fragments• Rapid one tube 5 minute protocolChargeSwitch PCR Clean Up Kit is the fastest and most effective method of removing primers unincorporated dNTPs thermostable polymerases and salts Figure 1 This kit is scalable for use on liquid handling robots This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory Please see the Accessories Chapter Chapter 6 to select the best magnetic accessory for your needs
    https://www.bioz.com/result/chargeswitch pcr clean up/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    chargeswitch pcr clean up - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: A Set of Multiplex Polymerase Chain Reactions for Genomic Detection of Nine Edible Insect Species in Foods
    Article Snippet: .. Successively, amplicons were purified using a commercial purification kit (ChargeSwitch PCR Clean-Up Kit, Invitrogen, Grand Island, NY) and sequenced (ABI Prism 310 Genetic Analyser, Applied Biosystems, Foster City, CA) to confirm the identity of the insect species and of the PCR amplified products. ..

    Article Title: Multi-omics of the gut microbial ecosystem in inflammatory bowel diseases
    Article Snippet: .. After short molecule and random hexamer removal using ChargeSwitch (CS12000, Thermo Fisher), molecules were amplified and tagged with a BC12-V8A2 construct using AccuPrimeTM Taq polymerase and cleaned with ChargeSwitch kit. .. The resulting viral amplicons were normalized, pooled, and made into an Illumina library without shearing.

    Article Title: Multi-omics of the gut microbial ecosystem in inflammatory bowel diseases
    Article Snippet: .. After short molecule and random hexamer removal using ChargeSwitch (CS12000, Thermo Fisher), molecules were amplified and tagged with a BC12-V8A2 construct using AccuPrimeTM Taq polymerase and cleaned with ChargeSwitch kit.). .. Pools of barcoded RNAs were converted to Illumina cDNA libraries in two main steps: (i) reverse transcription of the RNA using a primer designed to the constant region of the barcoded adaptor with addition of an adaptor to the 3′ end of the cDNA by template switching using SMARTScribe (Clontech) as described ; (ii) PCR amplification using primers whose 5′ ends target the constant regions of the 3′ or 5′ adaptors and whose 3′ ends contain the full Illumina P5 or P7 sequences. cDNA libraries were sequenced on the Illumina HiSeq2500 platform to generate ~13 million paired end reads.

    Nick Translation:

    Article Title: Repurposing the CRISPR-Cas9 system for targeted DNA methylation
    Article Snippet: .. Fluorescent in situ hybridization The Cy3-labeled probe for the plasmid encoding dCas9-DNMT3A was prepared using Nick Translation Kit (Roche, Indianapolis, Indiana, USA) and subsequently purified with the ChargeSwitch PCR Clean-Up Kit (Invitrogen, Paisley, UK) according to the manufacturer's protocols. .. For detection of genomic DNA, the centromeric chromosome 7 enumeration probe was used (Vysis CEP7 SpectrumGreen; Abbott Molecular, Des Plaines, IL, USA).

    Infection:

    Article Title: Early Mechanisms of Pathobiology Are Revealed by Transcriptional Temporal Dynamics in Hippocampal CA1 Neurons of Prion Infected Mice
    Article Snippet: .. A total of 2 ng of total RNA from 4 infected and 4 control mouse samples for each time point were reverse transcribed separately using the High Capacity cDNA reverse transcription kit (Life Technologies Inc.). cDNA was purified using the ChargeSwitch PCR Clean-Up Kit (Life Technologies Inc.) as per manufacturer's recommendations. .. Concentration and rough quality was assessed using the Nanodrop ND-1000 Spectrophotometer (Thermo Scientific).

    Purification:

    Article Title: A Set of Multiplex Polymerase Chain Reactions for Genomic Detection of Nine Edible Insect Species in Foods
    Article Snippet: .. Successively, amplicons were purified using a commercial purification kit (ChargeSwitch PCR Clean-Up Kit, Invitrogen, Grand Island, NY) and sequenced (ABI Prism 310 Genetic Analyser, Applied Biosystems, Foster City, CA) to confirm the identity of the insect species and of the PCR amplified products. ..

    Article Title: Biosynthesis of the Escherichia coli K1 group 2 polysialic acid capsule occurs within a protected cytoplasmic compartment
    Article Snippet: .. PCR products were purified using the ChargeSwitch PCR Clean-Up Kit (Invitrogen) followed by restriction digestion. ..

    Article Title: Shifts in the bacterial community composition along deep soil profiles in monospecific and mixed stands of Eucalyptus grandis and Acacia mangium
    Article Snippet: .. PCR products were purified with the ChargeSwitch® PCR Clean-Up kit (Life Technologies) and subsequently sequenced by the Ion Torrent Personal Genome Machine System (PGM) available at the Microbiology Laboratory of the National Centre for Environmental Research, Assessment and Impact Evaluation—EMBRAPA (Jaguariúna, São Paulo, Brazil). .. Sequence analysis was conducted using QIIME (Quantitative Insights into Microbial Ecology) software [ ].

    Article Title: Early Mechanisms of Pathobiology Are Revealed by Transcriptional Temporal Dynamics in Hippocampal CA1 Neurons of Prion Infected Mice
    Article Snippet: .. A total of 2 ng of total RNA from 4 infected and 4 control mouse samples for each time point were reverse transcribed separately using the High Capacity cDNA reverse transcription kit (Life Technologies Inc.). cDNA was purified using the ChargeSwitch PCR Clean-Up Kit (Life Technologies Inc.) as per manufacturer's recommendations. .. Concentration and rough quality was assessed using the Nanodrop ND-1000 Spectrophotometer (Thermo Scientific).

    Article Title: Repurposing the CRISPR-Cas9 system for targeted DNA methylation
    Article Snippet: .. Fluorescent in situ hybridization The Cy3-labeled probe for the plasmid encoding dCas9-DNMT3A was prepared using Nick Translation Kit (Roche, Indianapolis, Indiana, USA) and subsequently purified with the ChargeSwitch PCR Clean-Up Kit (Invitrogen, Paisley, UK) according to the manufacturer's protocols. .. For detection of genomic DNA, the centromeric chromosome 7 enumeration probe was used (Vysis CEP7 SpectrumGreen; Abbott Molecular, Des Plaines, IL, USA).

    Article Title: Severe perinatal hydrops fetalis in genome edited pigs with a biallelic five base pair deletion of the Marfan syndrome gene, FBN1
    Article Snippet: .. The PCR settings were as followed: 95°C for 30 s, 30 cycles of 95°C for 20 s, 60°C for 1 min and 68°C for 30 s, and a further 68°C for 5 min. PCR products were purified using the Chargeswitch PCR Clean-up kit (Thermo Fisher Scientific, Waltham, MA USA), before quantifying with a NanoDrop spectrophotometer (Thermo Fisher Scientific) and sequencing. .. Samples were sent for chain termination sequencing by Edinburgh Genomics (University of Edinburgh, UK).

    Polymerase Chain Reaction:

    Article Title: A Set of Multiplex Polymerase Chain Reactions for Genomic Detection of Nine Edible Insect Species in Foods
    Article Snippet: .. Successively, amplicons were purified using a commercial purification kit (ChargeSwitch PCR Clean-Up Kit, Invitrogen, Grand Island, NY) and sequenced (ABI Prism 310 Genetic Analyser, Applied Biosystems, Foster City, CA) to confirm the identity of the insect species and of the PCR amplified products. ..

    Article Title: Biosynthesis of the Escherichia coli K1 group 2 polysialic acid capsule occurs within a protected cytoplasmic compartment
    Article Snippet: .. PCR products were purified using the ChargeSwitch PCR Clean-Up Kit (Invitrogen) followed by restriction digestion. ..

    Article Title: Shifts in the bacterial community composition along deep soil profiles in monospecific and mixed stands of Eucalyptus grandis and Acacia mangium
    Article Snippet: .. PCR products were purified with the ChargeSwitch® PCR Clean-Up kit (Life Technologies) and subsequently sequenced by the Ion Torrent Personal Genome Machine System (PGM) available at the Microbiology Laboratory of the National Centre for Environmental Research, Assessment and Impact Evaluation—EMBRAPA (Jaguariúna, São Paulo, Brazil). .. Sequence analysis was conducted using QIIME (Quantitative Insights into Microbial Ecology) software [ ].

    Article Title: Early Mechanisms of Pathobiology Are Revealed by Transcriptional Temporal Dynamics in Hippocampal CA1 Neurons of Prion Infected Mice
    Article Snippet: .. A total of 2 ng of total RNA from 4 infected and 4 control mouse samples for each time point were reverse transcribed separately using the High Capacity cDNA reverse transcription kit (Life Technologies Inc.). cDNA was purified using the ChargeSwitch PCR Clean-Up Kit (Life Technologies Inc.) as per manufacturer's recommendations. .. Concentration and rough quality was assessed using the Nanodrop ND-1000 Spectrophotometer (Thermo Scientific).

    Article Title: Repurposing the CRISPR-Cas9 system for targeted DNA methylation
    Article Snippet: .. Fluorescent in situ hybridization The Cy3-labeled probe for the plasmid encoding dCas9-DNMT3A was prepared using Nick Translation Kit (Roche, Indianapolis, Indiana, USA) and subsequently purified with the ChargeSwitch PCR Clean-Up Kit (Invitrogen, Paisley, UK) according to the manufacturer's protocols. .. For detection of genomic DNA, the centromeric chromosome 7 enumeration probe was used (Vysis CEP7 SpectrumGreen; Abbott Molecular, Des Plaines, IL, USA).

    Article Title: Severe perinatal hydrops fetalis in genome edited pigs with a biallelic five base pair deletion of the Marfan syndrome gene, FBN1
    Article Snippet: .. The PCR settings were as followed: 95°C for 30 s, 30 cycles of 95°C for 20 s, 60°C for 1 min and 68°C for 30 s, and a further 68°C for 5 min. PCR products were purified using the Chargeswitch PCR Clean-up kit (Thermo Fisher Scientific, Waltham, MA USA), before quantifying with a NanoDrop spectrophotometer (Thermo Fisher Scientific) and sequencing. .. Samples were sent for chain termination sequencing by Edinburgh Genomics (University of Edinburgh, UK).

    Random Hexamer Labeling:

    Article Title: Multi-omics of the gut microbial ecosystem in inflammatory bowel diseases
    Article Snippet: .. After short molecule and random hexamer removal using ChargeSwitch (CS12000, Thermo Fisher), molecules were amplified and tagged with a BC12-V8A2 construct using AccuPrimeTM Taq polymerase and cleaned with ChargeSwitch kit. .. The resulting viral amplicons were normalized, pooled, and made into an Illumina library without shearing.

    Article Title: Multi-omics of the gut microbial ecosystem in inflammatory bowel diseases
    Article Snippet: .. After short molecule and random hexamer removal using ChargeSwitch (CS12000, Thermo Fisher), molecules were amplified and tagged with a BC12-V8A2 construct using AccuPrimeTM Taq polymerase and cleaned with ChargeSwitch kit.). .. Pools of barcoded RNAs were converted to Illumina cDNA libraries in two main steps: (i) reverse transcription of the RNA using a primer designed to the constant region of the barcoded adaptor with addition of an adaptor to the 3′ end of the cDNA by template switching using SMARTScribe (Clontech) as described ; (ii) PCR amplification using primers whose 5′ ends target the constant regions of the 3′ or 5′ adaptors and whose 3′ ends contain the full Illumina P5 or P7 sequences. cDNA libraries were sequenced on the Illumina HiSeq2500 platform to generate ~13 million paired end reads.

    Construct:

    Article Title: Multi-omics of the gut microbial ecosystem in inflammatory bowel diseases
    Article Snippet: .. After short molecule and random hexamer removal using ChargeSwitch (CS12000, Thermo Fisher), molecules were amplified and tagged with a BC12-V8A2 construct using AccuPrimeTM Taq polymerase and cleaned with ChargeSwitch kit. .. The resulting viral amplicons were normalized, pooled, and made into an Illumina library without shearing.

    Article Title: Multi-omics of the gut microbial ecosystem in inflammatory bowel diseases
    Article Snippet: .. After short molecule and random hexamer removal using ChargeSwitch (CS12000, Thermo Fisher), molecules were amplified and tagged with a BC12-V8A2 construct using AccuPrimeTM Taq polymerase and cleaned with ChargeSwitch kit.). .. Pools of barcoded RNAs were converted to Illumina cDNA libraries in two main steps: (i) reverse transcription of the RNA using a primer designed to the constant region of the barcoded adaptor with addition of an adaptor to the 3′ end of the cDNA by template switching using SMARTScribe (Clontech) as described ; (ii) PCR amplification using primers whose 5′ ends target the constant regions of the 3′ or 5′ adaptors and whose 3′ ends contain the full Illumina P5 or P7 sequences. cDNA libraries were sequenced on the Illumina HiSeq2500 platform to generate ~13 million paired end reads.

    Sequencing:

    Article Title: Severe perinatal hydrops fetalis in genome edited pigs with a biallelic five base pair deletion of the Marfan syndrome gene, FBN1
    Article Snippet: .. The PCR settings were as followed: 95°C for 30 s, 30 cycles of 95°C for 20 s, 60°C for 1 min and 68°C for 30 s, and a further 68°C for 5 min. PCR products were purified using the Chargeswitch PCR Clean-up kit (Thermo Fisher Scientific, Waltham, MA USA), before quantifying with a NanoDrop spectrophotometer (Thermo Fisher Scientific) and sequencing. .. Samples were sent for chain termination sequencing by Edinburgh Genomics (University of Edinburgh, UK).

    Spectrophotometry:

    Article Title: Severe perinatal hydrops fetalis in genome edited pigs with a biallelic five base pair deletion of the Marfan syndrome gene, FBN1
    Article Snippet: .. The PCR settings were as followed: 95°C for 30 s, 30 cycles of 95°C for 20 s, 60°C for 1 min and 68°C for 30 s, and a further 68°C for 5 min. PCR products were purified using the Chargeswitch PCR Clean-up kit (Thermo Fisher Scientific, Waltham, MA USA), before quantifying with a NanoDrop spectrophotometer (Thermo Fisher Scientific) and sequencing. .. Samples were sent for chain termination sequencing by Edinburgh Genomics (University of Edinburgh, UK).

    In Situ Hybridization:

    Article Title: Repurposing the CRISPR-Cas9 system for targeted DNA methylation
    Article Snippet: .. Fluorescent in situ hybridization The Cy3-labeled probe for the plasmid encoding dCas9-DNMT3A was prepared using Nick Translation Kit (Roche, Indianapolis, Indiana, USA) and subsequently purified with the ChargeSwitch PCR Clean-Up Kit (Invitrogen, Paisley, UK) according to the manufacturer's protocols. .. For detection of genomic DNA, the centromeric chromosome 7 enumeration probe was used (Vysis CEP7 SpectrumGreen; Abbott Molecular, Des Plaines, IL, USA).

    Plasmid Preparation:

    Article Title: Repurposing the CRISPR-Cas9 system for targeted DNA methylation
    Article Snippet: .. Fluorescent in situ hybridization The Cy3-labeled probe for the plasmid encoding dCas9-DNMT3A was prepared using Nick Translation Kit (Roche, Indianapolis, Indiana, USA) and subsequently purified with the ChargeSwitch PCR Clean-Up Kit (Invitrogen, Paisley, UK) according to the manufacturer's protocols. .. For detection of genomic DNA, the centromeric chromosome 7 enumeration probe was used (Vysis CEP7 SpectrumGreen; Abbott Molecular, Des Plaines, IL, USA).

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  • 99
    Thermo Fisher chargeswitch pcr clean up kit
    Chargeswitch Pcr Clean Up Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chargeswitch pcr clean up kit/product/Thermo Fisher
    Average 99 stars, based on 53 article reviews
    Price from $9.99 to $1999.99
    chargeswitch pcr clean up kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    88
    Thermo Fisher chargeswitch pro pcr clean up kit
    Chargeswitch Pro Pcr Clean Up Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chargeswitch pro pcr clean up kit/product/Thermo Fisher
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    chargeswitch pro pcr clean up kit - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

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