mouse anti cep55  (Santa Cruz Biotechnology)

Journal: Scientific Reports

Article Title: CBX2 promoted oral squamous cell carcinoma via increasing CEP55/NF-κB/METTL3/SHP2 signaling induced metastasis/proliferation and angiogenesis

doi: 10.1038/s41598-025-31475-3

Figure Lengend Snippet: Bioinformatics analysis reveals the crucial role of the CBX2-NF-κB-METTL3-SHP2 pathway in oral squamous cell carcinoma. ( A ) Boxplot of sample correction; ( B ) PCA plot of differential samples; ( C ) Volcano plot of differential genes; ( D ) Ordered plot of differential genes; ( E ) Bar chart of GO enrichment analysis; ( F ) Bubble plot of KEGG enrichment analysis; ( G ) Lollipop chart of KEGG enrichment analysis; ( H ) Scatter plot of CBX2 and CEP55 correlation; ( I ) Scatter plot of CEP55 and NF-κB correlation; ( J ) Scatter plot of NF-κB and METTL3 correlation; ( K ) Scatter plot of METTL3 and SHP2 (PTPN11) correlation; ( L ) CBX2 expression levels and patient survival analysis.

Article Snippet: To investigate the interaction between CBX2 and CEP55, anti-CBX2 antibody (Abcam ab235305) and anti-CEP55 antibody (CST #81693) were used as primary antibodies.

Techniques: Expressing

Journal: Scientific Reports

Article Title: CBX2 promoted oral squamous cell carcinoma via increasing CEP55/NF-κB/METTL3/SHP2 signaling induced metastasis/proliferation and angiogenesis

doi: 10.1038/s41598-025-31475-3

Figure Lengend Snippet: Western blot and Co-IP results indicate that knockdown of CBX2, activation of NF-κB, inhibition of METTL3, overexpression of METTL3, and Inhibition of SHP2 all upregulate or downregulate downstream signaling molecules. ( A ) Western Blot analysis of protein bands for CBX2, CEP55, p-NF-κB, METTL3, TGFβ1, and p-SHP2 in the NC group, CBX2-KD group, CBX2-KD-E1351 group, CBX2-KD-E1351-STM2457 group, CBX2-KD-E1351-STM2457-METTL3-OE group, and CBX2-KD-E1351-STM2457-METTL3-OE-PHPS1 group, as well as statistical analysis graphs of relative protein expression levels. GAPDH was as a control protein; ( B ) Western Blot analysis of protein bands for p-PI3K, Slug, and Snail, along with statistical analysis graphs of relative protein expression levels. GAPDH was as the control protein; ( C ) Statistical analysis chart of the Co-IP experiment analyzing the interaction between endogenous CBX2 and CEP55 in SCC-25 cells. N = 6; Data are expressed as mean ± standard deviation; * P < 0.05; ** P < 0.01; ns P > 0.05.

Article Snippet: To investigate the interaction between CBX2 and CEP55, anti-CBX2 antibody (Abcam ab235305) and anti-CEP55 antibody (CST #81693) were used as primary antibodies.

Techniques: Western Blot, Co-Immunoprecipitation Assay, Knockdown, Activation Assay, Inhibition, Over Expression, Expressing, Control, Standard Deviation

Journal: Scientific Reports

Article Title: CBX2 promoted oral squamous cell carcinoma via increasing CEP55/NF-κB/METTL3/SHP2 signaling induced metastasis/proliferation and angiogenesis

doi: 10.1038/s41598-025-31475-3

Figure Lengend Snippet: Schematic illustration of CBX2 promoting oral squamous cell carcinoma via increasing CEP55/NF-κB/METTL3/SHP2 signaling, inducing metastasis/proliferation, and angiogenesis.

Article Snippet: To investigate the interaction between CBX2 and CEP55, anti-CBX2 antibody (Abcam ab235305) and anti-CEP55 antibody (CST #81693) were used as primary antibodies.

Techniques:

Journal: bioRxiv

Article Title: Genetic Ablation and Multi-Omics Profiling Reveal CEP55 as a Key Driver of Tumorigenesis in Diverse Cancer Models

doi: 10.1101/2025.10.28.685219

Figure Lengend Snippet:

Article Snippet: Tissues and cells were lysed in RIPA or urea buffers, protein quantified via BCA assay, electrophoresed via SDS-PAGE, transferred to nitrocellulose membranes, and probed with antibodies listed: CEP55 CST (D1L4H) Rabbit mAb #81693 1:1000; Cep55 Santa Cruz biotechnology, sc-374051, 1:500; Vinculin Cell Signaling Technology #13901, 1:2000; β-Actin BD Pharmingen#612656, 1:2000; β-Catenin Cell Signaling Technology 9582, 1:1000; pERK1/2 (T202/Y204) Cell Signaling Technology #4370, 1:1000; ERK1/2Cell Signaling Technology #4695, 1:1000; pAKTS473 Cell Signaling Technology #4060, 1:1000; AKT Cell Signaling Technology #9271, 1:1000; MYC (Y69) Abcam #ab32072, 1:1000; Rabbit Secondary (peroxidase) Sigma Aldrich #A0545, 1:5000; Mouse Secondary (peroxidase) Sigma Aldrich #A9044, 1:5000.

Techniques: Expressing

Journal: bioRxiv

Article Title: Genetic Ablation and Multi-Omics Profiling Reveal CEP55 as a Key Driver of Tumorigenesis in Diverse Cancer Models

doi: 10.1101/2025.10.28.685219

Figure Lengend Snippet:

Article Snippet: Tissues and cells were lysed in RIPA or urea buffers, protein quantified via BCA assay, electrophoresed via SDS-PAGE, transferred to nitrocellulose membranes, and probed with antibodies listed: CEP55 CST (D1L4H) Rabbit mAb #81693 1:1000; Cep55 Santa Cruz biotechnology, sc-374051, 1:500; Vinculin Cell Signaling Technology #13901, 1:2000; β-Actin BD Pharmingen#612656, 1:2000; β-Catenin Cell Signaling Technology 9582, 1:1000; pERK1/2 (T202/Y204) Cell Signaling Technology #4370, 1:1000; ERK1/2Cell Signaling Technology #4695, 1:1000; pAKTS473 Cell Signaling Technology #4060, 1:1000; AKT Cell Signaling Technology #9271, 1:1000; MYC (Y69) Abcam #ab32072, 1:1000; Rabbit Secondary (peroxidase) Sigma Aldrich #A0545, 1:5000; Mouse Secondary (peroxidase) Sigma Aldrich #A9044, 1:5000.

Techniques: Expressing