centrifuge protein concentrator  (Millipore)


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    Structured Review

    Millipore centrifuge protein concentrator
    Centrifuge Protein Concentrator, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/centrifuge protein concentrator/product/Millipore
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    centrifuge protein concentrator - by Bioz Stars, 2020-04
    88/100 stars

    Related Products / Commonly Used Together

    kindlin-3
    tris-hcl
    nacl

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    Related Articles

    Centrifugation:

    Article Title: Biophysical Analysis of Kindlin-3 Reveals an Elongated Conformation and Maps Integrin Binding to the Membrane-distal ?-Subunit NPXY Motif
    Article Snippet: The recombinant protein was purified using three chromatographic steps after the lysate was clarified by centrifugation at 48,000 × g for 1 h at 4 °C. .. The eluant containing Kindlin-3 was pooled and buffer-exchanged into 20 mm Tris-HCl, pH 7.5, 200 mm NaCl via a series of dilutions into buffer, and the sample was concentrated using a centrifuge protein concentrator (Millipore) with a 50-kDa molecular weight cut-off (MWCO).

    Filtration:

    Article Title: Biophysical Analysis of Kindlin-3 Reveals an Elongated Conformation and Maps Integrin Binding to the Membrane-distal ?-Subunit NPXY Motif
    Article Snippet: .. The protein was concentrated using a centrifuge protein concentrator (Millipore) with a 50-kDa MWCO to ∼15 mg/ml in the gel filtration buffer, flash-frozen in liquid nitrogen, and stored at −80 °C until used. .. The protein concentration was assessed spectroscopically using a calculated extinction coefficient (ϵ) of 109,320 m −1 cm−1 (assuming all Cys residues were reduced).

    Purification:

    Article Title: Biophysical Analysis of Kindlin-3 Reveals an Elongated Conformation and Maps Integrin Binding to the Membrane-distal ?-Subunit NPXY Motif
    Article Snippet: Paragraph title: Recombinant Kindlin-3 Purification for Biophysical Characterization ... The protein composition of the fractionated eluant was assessed by SDS-PAGE and Western blotting, and those containing Kindlin-3 were pooled and concentrated using a centrifuge protein concentrator (Millipore) with a 50-kDa MWCO to 0.5 ml-2 ml sample size.

    Incubation:

    Article Title: Biophysical Analysis of Kindlin-3 Reveals an Elongated Conformation and Maps Integrin Binding to the Membrane-distal ?-Subunit NPXY Motif
    Article Snippet: The supernatant was incubated with precharged and equilibrated nickel-Sepharose for 1–2 h at 4 °C. .. The eluant containing Kindlin-3 was pooled and buffer-exchanged into 20 mm Tris-HCl, pH 7.5, 200 mm NaCl via a series of dilutions into buffer, and the sample was concentrated using a centrifuge protein concentrator (Millipore) with a 50-kDa molecular weight cut-off (MWCO).

    Protein Concentration:

    Article Title: Biophysical Analysis of Kindlin-3 Reveals an Elongated Conformation and Maps Integrin Binding to the Membrane-distal ?-Subunit NPXY Motif
    Article Snippet: The protein was concentrated using a centrifuge protein concentrator (Millipore) with a 50-kDa MWCO to ∼15 mg/ml in the gel filtration buffer, flash-frozen in liquid nitrogen, and stored at −80 °C until used. .. The protein concentration was assessed spectroscopically using a calculated extinction coefficient (ϵ) of 109,320 m −1 cm−1 (assuming all Cys residues were reduced).

    Western Blot:

    Article Title: Biophysical Analysis of Kindlin-3 Reveals an Elongated Conformation and Maps Integrin Binding to the Membrane-distal ?-Subunit NPXY Motif
    Article Snippet: .. The protein composition of the fractionated eluant was assessed by SDS-PAGE and Western blotting, and those containing Kindlin-3 were pooled and concentrated using a centrifuge protein concentrator (Millipore) with a 50-kDa MWCO to 0.5 ml-2 ml sample size. ..

    Article Title: Biophysical Analysis of Kindlin-3 Reveals an Elongated Conformation and Maps Integrin Binding to the Membrane-distal ?-Subunit NPXY Motif
    Article Snippet: The protein composition of the eluant was assessed by SDS-PAGE and, in the first instance, Western blotting using an anti-His5 antibody. .. The eluant containing Kindlin-3 was pooled and buffer-exchanged into 20 mm Tris-HCl, pH 7.5, 200 mm NaCl via a series of dilutions into buffer, and the sample was concentrated using a centrifuge protein concentrator (Millipore) with a 50-kDa molecular weight cut-off (MWCO).

    Injection:

    Article Title: Biophysical Analysis of Kindlin-3 Reveals an Elongated Conformation and Maps Integrin Binding to the Membrane-distal ?-Subunit NPXY Motif
    Article Snippet: The protein composition of the fractionated eluant was assessed by SDS-PAGE and Western blotting, and those containing Kindlin-3 were pooled and concentrated using a centrifuge protein concentrator (Millipore) with a 50-kDa MWCO to 0.5 ml-2 ml sample size. .. The protein was injected onto a pre-equilibrated Superdex S200 (16/60) or (10/30) (GE Healthcare) in 20 mm Tris-HCl, pH 7.5, 250 mm NaCl, and 1 mm DTT at a rate of 1 ml/min.

    Recombinant:

    Article Title: Biophysical Analysis of Kindlin-3 Reveals an Elongated Conformation and Maps Integrin Binding to the Membrane-distal ?-Subunit NPXY Motif
    Article Snippet: Paragraph title: Recombinant Kindlin-3 Purification for Biophysical Characterization ... The protein composition of the fractionated eluant was assessed by SDS-PAGE and Western blotting, and those containing Kindlin-3 were pooled and concentrated using a centrifuge protein concentrator (Millipore) with a 50-kDa MWCO to 0.5 ml-2 ml sample size.

    SDS Page:

    Article Title: Biophysical Analysis of Kindlin-3 Reveals an Elongated Conformation and Maps Integrin Binding to the Membrane-distal ?-Subunit NPXY Motif
    Article Snippet: .. The protein composition of the fractionated eluant was assessed by SDS-PAGE and Western blotting, and those containing Kindlin-3 were pooled and concentrated using a centrifuge protein concentrator (Millipore) with a 50-kDa MWCO to 0.5 ml-2 ml sample size. ..

    Article Title: Biophysical Analysis of Kindlin-3 Reveals an Elongated Conformation and Maps Integrin Binding to the Membrane-distal ?-Subunit NPXY Motif
    Article Snippet: The protein composition of the eluant was assessed by SDS-PAGE and, in the first instance, Western blotting using an anti-His5 antibody. .. The eluant containing Kindlin-3 was pooled and buffer-exchanged into 20 mm Tris-HCl, pH 7.5, 200 mm NaCl via a series of dilutions into buffer, and the sample was concentrated using a centrifuge protein concentrator (Millipore) with a 50-kDa molecular weight cut-off (MWCO).

    Molecular Weight:

    Article Title: Biophysical Analysis of Kindlin-3 Reveals an Elongated Conformation and Maps Integrin Binding to the Membrane-distal ?-Subunit NPXY Motif
    Article Snippet: The eluant containing Kindlin-3 was pooled and buffer-exchanged into 20 mm Tris-HCl, pH 7.5, 200 mm NaCl via a series of dilutions into buffer, and the sample was concentrated using a centrifuge protein concentrator (Millipore) with a 50-kDa molecular weight cut-off (MWCO). .. The protein composition of the fractionated eluant was assessed by SDS-PAGE and Western blotting, and those containing Kindlin-3 were pooled and concentrated using a centrifuge protein concentrator (Millipore) with a 50-kDa MWCO to 0.5 ml-2 ml sample size.

    Article Title: Biophysical Analysis of Kindlin-3 Reveals an Elongated Conformation and Maps Integrin Binding to the Membrane-distal ?-Subunit NPXY Motif
    Article Snippet: .. The eluant containing Kindlin-3 was pooled and buffer-exchanged into 20 mm Tris-HCl, pH 7.5, 200 mm NaCl via a series of dilutions into buffer, and the sample was concentrated using a centrifuge protein concentrator (Millipore) with a 50-kDa molecular weight cut-off (MWCO). .. The buffer-exchanged protein solution was applied onto a pre-equilibrated HiTrap heparin HP column (5 ml column volume, GE Healthcare).

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  • 88
    Millipore centrifuge protein concentrator
    Centrifuge Protein Concentrator, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/centrifuge protein concentrator/product/Millipore
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    centrifuge protein concentrator - by Bioz Stars, 2020-04
    88/100 stars
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