centricon protein concentrator  (Millipore)


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    Structured Review

    Millipore centricon protein concentrator
    Centricon Protein Concentrator, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/centricon protein concentrator/product/Millipore
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    centricon protein concentrator - by Bioz Stars, 2020-04
    93/100 stars

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    Related Articles

    Transduction:

    Article Title: Independent and cooperative roles of tumor necrosis factor-?, nuclear factor-?B, and bone morphogenetic protein-2 in regulation of metastasis and osteomimicry of prostate cancer cells and differentiation and mineralization of MC3T3-E1 osteoblast-like cells
    Article Snippet: Prostate cancer cells (1 × 106 ) untreated or transduced with 20 MOI of adenovirus constructs (Adv-Null or Adv-dnIκBα) were stimulated for 2 h either with recombinant human TNF-α (10 ng/mL) or BMP-2 (200 ng/mL) alone or in combination with 3 μg/mL of the BMP-2 antagonist Noggin. .. The media were removed and cells were subsequently cultured in phenol-red-free RPMI medium containing 1% charcoal-stripped serum for 48 h. The PC cell conditioned media (CM) were centrifuged at 4°C to remove cellular debris and concentrated using a Centricon protein concentrator (Millipore, Billerica, MA, USA).

    Article Title: PI3K/Akt-Dependent Transcriptional Regulation and Activation of BMP-2-Smad Signaling by NF-?B in Metastatic Prostate Cancer Cells
    Article Snippet: The cell culture media were collected after 48 hr and were concentrated in the presence of protease inhibitors at 4°C using Centricon protein concentrator (Millipore, Billerica, MA). .. In another set of experiments, phosphorylation of Smad and Akt proteins was examined in C4-2B cells transduced with 20 MOIs of control vector prior to the addition of rhBMP-2 200 ng/ml for 1 hr.

    Centrifugation:

    Article Title: Modulation of microtubule dynamics by a TIR domain protein from the intracellular pathogen Brucella melitensis
    Article Snippet: The induced culture was then grown at 25°C for 5 h. Cells were collected by centrifugation at 5000 g for 10 min and resuspended in sonication buffer containing 50 mM Tris/HCl (pH 8.0), 1 M NaCl, 1 mM EDTA and protease inhibitor cocktail (Pierce). .. The eluted protein was then subjected to SP Sepharose (Sigma) ion-exchange chromatography to remove maltose followed by concentration using a Centricon protein concentrator (Millipore).

    Article Title: Active Evasion of CTL Mediated Killing and Low Quality Responding CD8+ T Cells Contribute to Persistence of Brucellosis
    Article Snippet: Cells were collected by centrifugation and resuspended in sonication buffer containing 50 mM Tris-HCI [pH 8.0], 1 M NaCI, 1 mM EDTA and 1X protease inhibitor cocktail (Pierce). .. The eluted protein was then subjected to Genenase I protease site to cleave TcpB from MBP followed by SP Sepharose (Sigma) ion exchange chromatography to remove maltose followed by concentration using a centricon protein concentrator (Millipore).

    Article Title: Substrates and Inhibitors of SAMHD1
    Article Snippet: Cell debris was removed by centrifugation at 49,000 × g for 15 min, and lysate was incubated overnight at 4°C with 1.5 mL of Glutathione Sepharose® 4B bead slurry (GE Healthcare). .. A Millipore Centricon protein concentrator (45 MWCO) was used to concentrate the protein and for buffer exchanges.

    Construct:

    Article Title: Independent and cooperative roles of tumor necrosis factor-?, nuclear factor-?B, and bone morphogenetic protein-2 in regulation of metastasis and osteomimicry of prostate cancer cells and differentiation and mineralization of MC3T3-E1 osteoblast-like cells
    Article Snippet: Prostate cancer cells (1 × 106 ) untreated or transduced with 20 MOI of adenovirus constructs (Adv-Null or Adv-dnIκBα) were stimulated for 2 h either with recombinant human TNF-α (10 ng/mL) or BMP-2 (200 ng/mL) alone or in combination with 3 μg/mL of the BMP-2 antagonist Noggin. .. The media were removed and cells were subsequently cultured in phenol-red-free RPMI medium containing 1% charcoal-stripped serum for 48 h. The PC cell conditioned media (CM) were centrifuged at 4°C to remove cellular debris and concentrated using a Centricon protein concentrator (Millipore, Billerica, MA, USA).

    Incubation:

    Article Title: Substrates and Inhibitors of SAMHD1
    Article Snippet: Cell debris was removed by centrifugation at 49,000 × g for 15 min, and lysate was incubated overnight at 4°C with 1.5 mL of Glutathione Sepharose® 4B bead slurry (GE Healthcare). .. A Millipore Centricon protein concentrator (45 MWCO) was used to concentrate the protein and for buffer exchanges.

    Expressing:

    Article Title: Independent and cooperative roles of tumor necrosis factor-?, nuclear factor-?B, and bone morphogenetic protein-2 in regulation of metastasis and osteomimicry of prostate cancer cells and differentiation and mineralization of MC3T3-E1 osteoblast-like cells
    Article Snippet: The media were removed and cells were subsequently cultured in phenol-red-free RPMI medium containing 1% charcoal-stripped serum for 48 h. The PC cell conditioned media (CM) were centrifuged at 4°C to remove cellular debris and concentrated using a Centricon protein concentrator (Millipore, Billerica, MA, USA). .. In addition to its effects on mineralization in preosteoblast MC 3T3-E1 cells, modulation of receptor activator of NF-κB ligand ( RANKL ) gene expression by CM was examined by RT-PCR in MC 3T3-E1 and the preosteoclast RAW 264.7 cells.

    Article Title: The Brucella effector protein TcpB induces degradation of inflammatory caspases and thereby subverts non-canonical inflammasome activation in macrophages
    Article Snippet: Paragraph title: Protein expression and purification ... The eluted protein was concentrated using a Centricon protein concentrator (Millipore) and dialyzed in a buffer containing 50 m m Tris-HCl, 100 m m NaCl, and 10% glycerol.

    BIA-KA:

    Article Title: PI3K/Akt-Dependent Transcriptional Regulation and Activation of BMP-2-Smad Signaling by NF-?B in Metastatic Prostate Cancer Cells
    Article Snippet: The cell culture media were collected after 48 hr and were concentrated in the presence of protease inhibitors at 4°C using Centricon protein concentrator (Millipore, Billerica, MA). .. Total protein concentrations of cell lysates were quantified using the BCA protein assay kit (Pierce), fractionated (20 µg/lane) onto 12% SDS–polyacrylamide gels, and subsequently electroblotted by semi-dry transfer technique onto 0.2 µm nitrocellulose membranes.

    Western Blot:

    Article Title: PI3K/Akt-Dependent Transcriptional Regulation and Activation of BMP-2-Smad Signaling by NF-?B in Metastatic Prostate Cancer Cells
    Article Snippet: Paragraph title: Western blot Analysis ... The cell culture media were collected after 48 hr and were concentrated in the presence of protease inhibitors at 4°C using Centricon protein concentrator (Millipore, Billerica, MA).

    Over Expression:

    Article Title: Modulation of microtubule dynamics by a TIR domain protein from the intracellular pathogen Brucella melitensis
    Article Snippet: Paragraph title: Overexpression and purification of TcpB ... The eluted protein was then subjected to SP Sepharose (Sigma) ion-exchange chromatography to remove maltose followed by concentration using a Centricon protein concentrator (Millipore).

    Transfection:

    Article Title: PI3K/Akt-Dependent Transcriptional Regulation and Activation of BMP-2-Smad Signaling by NF-?B in Metastatic Prostate Cancer Cells
    Article Snippet: The cell culture media were collected after 48 hr and were concentrated in the presence of protease inhibitors at 4°C using Centricon protein concentrator (Millipore, Billerica, MA). .. To determine if NF-κB can activate Smad proteins independent of the BMP receptors, C4-2B cells were transfected with pEGFP-dnBMPRII, pIκBαM, pcDNA-p65, or a control pcDNA in presence of BMP-2 200 ng/ml as shown above.

    Protease Inhibitor:

    Article Title: Modulation of microtubule dynamics by a TIR domain protein from the intracellular pathogen Brucella melitensis
    Article Snippet: The induced culture was then grown at 25°C for 5 h. Cells were collected by centrifugation at 5000 g for 10 min and resuspended in sonication buffer containing 50 mM Tris/HCl (pH 8.0), 1 M NaCl, 1 mM EDTA and protease inhibitor cocktail (Pierce). .. The eluted protein was then subjected to SP Sepharose (Sigma) ion-exchange chromatography to remove maltose followed by concentration using a Centricon protein concentrator (Millipore).

    Article Title: Active Evasion of CTL Mediated Killing and Low Quality Responding CD8+ T Cells Contribute to Persistence of Brucellosis
    Article Snippet: Cells were collected by centrifugation and resuspended in sonication buffer containing 50 mM Tris-HCI [pH 8.0], 1 M NaCI, 1 mM EDTA and 1X protease inhibitor cocktail (Pierce). .. The eluted protein was then subjected to Genenase I protease site to cleave TcpB from MBP followed by SP Sepharose (Sigma) ion exchange chromatography to remove maltose followed by concentration using a centricon protein concentrator (Millipore).

    Article Title: Substrates and Inhibitors of SAMHD1
    Article Snippet: Cells were harvested and lysed in lysis buffer (50 mM Tris-HCl [pH 7.5], 500 mM NaCl, 2 mM EDTA, 1 mg/ml chicken egg white lysozyme, and one tablet of Roche Applied Science Complete protease inhibitor mixture) for 4 h on ice. .. A Millipore Centricon protein concentrator (45 MWCO) was used to concentrate the protein and for buffer exchanges.

    Cell Culture:

    Article Title: Independent and cooperative roles of tumor necrosis factor-?, nuclear factor-?B, and bone morphogenetic protein-2 in regulation of metastasis and osteomimicry of prostate cancer cells and differentiation and mineralization of MC3T3-E1 osteoblast-like cells
    Article Snippet: .. The media were removed and cells were subsequently cultured in phenol-red-free RPMI medium containing 1% charcoal-stripped serum for 48 h. The PC cell conditioned media (CM) were centrifuged at 4°C to remove cellular debris and concentrated using a Centricon protein concentrator (Millipore, Billerica, MA, USA). ..

    Article Title: Modulation of microtubule dynamics by a TIR domain protein from the intracellular pathogen Brucella melitensis
    Article Snippet: Briefly, 1 litre of E. coli BL21 cell culture harbouring the pMALTcpB or pMALTcpBG158A plasmid was induced with IPTG (isopropyl β-D-thiogalactopyranoside) to a final concentration of 0.5 mM when the D 600 reached 0.6. .. The eluted protein was then subjected to SP Sepharose (Sigma) ion-exchange chromatography to remove maltose followed by concentration using a Centricon protein concentrator (Millipore).

    Article Title: PI3K/Akt-Dependent Transcriptional Regulation and Activation of BMP-2-Smad Signaling by NF-?B in Metastatic Prostate Cancer Cells
    Article Snippet: .. The cell culture media were collected after 48 hr and were concentrated in the presence of protease inhibitors at 4°C using Centricon protein concentrator (Millipore, Billerica, MA). .. In another set of experiments, phosphorylation of Smad and Akt proteins was examined in C4-2B cells transduced with 20 MOIs of control vector prior to the addition of rhBMP-2 200 ng/ml for 1 hr.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Independent and cooperative roles of tumor necrosis factor-?, nuclear factor-?B, and bone morphogenetic protein-2 in regulation of metastasis and osteomimicry of prostate cancer cells and differentiation and mineralization of MC3T3-E1 osteoblast-like cells
    Article Snippet: The media were removed and cells were subsequently cultured in phenol-red-free RPMI medium containing 1% charcoal-stripped serum for 48 h. The PC cell conditioned media (CM) were centrifuged at 4°C to remove cellular debris and concentrated using a Centricon protein concentrator (Millipore, Billerica, MA, USA). .. In addition to its effects on mineralization in preosteoblast MC 3T3-E1 cells, modulation of receptor activator of NF-κB ligand ( RANKL ) gene expression by CM was examined by RT-PCR in MC 3T3-E1 and the preosteoclast RAW 264.7 cells.

    Sonication:

    Article Title: Modulation of microtubule dynamics by a TIR domain protein from the intracellular pathogen Brucella melitensis
    Article Snippet: The column was then washed with the sonication buffer followed by the same buffer containing decreasing concentrations of NaCl (750, 500, 250 or 100 mM). .. The eluted protein was then subjected to SP Sepharose (Sigma) ion-exchange chromatography to remove maltose followed by concentration using a Centricon protein concentrator (Millipore).

    Article Title: Active Evasion of CTL Mediated Killing and Low Quality Responding CD8+ T Cells Contribute to Persistence of Brucellosis
    Article Snippet: The column was then washed with the sonication buffer followed by the same buffer containing decreasing concentrations of NaCI (750, 500, 250 and 100 mM). .. The eluted protein was then subjected to Genenase I protease site to cleave TcpB from MBP followed by SP Sepharose (Sigma) ion exchange chromatography to remove maltose followed by concentration using a centricon protein concentrator (Millipore).

    Article Title: Biochemical and functional analysis of TIR domain containing protein from Brucella melitensis
    Article Snippet: The column was then washed with the sonication buffer followed by the same buffer containing decreasing concentrations of NaCI (750, 500, 250 and 100 mM). .. The eluted protein was concentrated using centricon protein concentrator (Millipore) and dialyzed in a buffer containing 50 mM Tris-HCI, 100 mM NaCI and 1mM EDTA.

    Article Title: The Brucella effector protein TcpB induces degradation of inflammatory caspases and thereby subverts non-canonical inflammasome activation in macrophages
    Article Snippet: The column was then washed with sonication buffer followed by the same buffer containing decreasing concentrations of NaCl (750, 500, 250, and 100 m m ). .. The eluted protein was concentrated using a Centricon protein concentrator (Millipore) and dialyzed in a buffer containing 50 m m Tris-HCl, 100 m m NaCl, and 10% glycerol.

    Recombinant:

    Article Title: Independent and cooperative roles of tumor necrosis factor-?, nuclear factor-?B, and bone morphogenetic protein-2 in regulation of metastasis and osteomimicry of prostate cancer cells and differentiation and mineralization of MC3T3-E1 osteoblast-like cells
    Article Snippet: Prostate cancer cells (1 × 106 ) untreated or transduced with 20 MOI of adenovirus constructs (Adv-Null or Adv-dnIκBα) were stimulated for 2 h either with recombinant human TNF-α (10 ng/mL) or BMP-2 (200 ng/mL) alone or in combination with 3 μg/mL of the BMP-2 antagonist Noggin. .. The media were removed and cells were subsequently cultured in phenol-red-free RPMI medium containing 1% charcoal-stripped serum for 48 h. The PC cell conditioned media (CM) were centrifuged at 4°C to remove cellular debris and concentrated using a Centricon protein concentrator (Millipore, Billerica, MA, USA).

    Article Title: Substrates and Inhibitors of SAMHD1
    Article Snippet: Paragraph title: Recombinant SAMHD1-GST purification ... A Millipore Centricon protein concentrator (45 MWCO) was used to concentrate the protein and for buffer exchanges.

    Ion Exchange Chromatography:

    Article Title: Modulation of microtubule dynamics by a TIR domain protein from the intracellular pathogen Brucella melitensis
    Article Snippet: .. The eluted protein was then subjected to SP Sepharose (Sigma) ion-exchange chromatography to remove maltose followed by concentration using a Centricon protein concentrator (Millipore). ..

    Article Title: Active Evasion of CTL Mediated Killing and Low Quality Responding CD8+ T Cells Contribute to Persistence of Brucellosis
    Article Snippet: .. The eluted protein was then subjected to Genenase I protease site to cleave TcpB from MBP followed by SP Sepharose (Sigma) ion exchange chromatography to remove maltose followed by concentration using a centricon protein concentrator (Millipore). ..

    Purification:

    Article Title: Modulation of microtubule dynamics by a TIR domain protein from the intracellular pathogen Brucella melitensis
    Article Snippet: Paragraph title: Overexpression and purification of TcpB ... The eluted protein was then subjected to SP Sepharose (Sigma) ion-exchange chromatography to remove maltose followed by concentration using a Centricon protein concentrator (Millipore).

    Article Title: Biochemical and functional analysis of TIR domain containing protein from Brucella melitensis
    Article Snippet: Paragraph title: Purification of MBP-TcpB ... The eluted protein was concentrated using centricon protein concentrator (Millipore) and dialyzed in a buffer containing 50 mM Tris-HCI, 100 mM NaCI and 1mM EDTA.

    Article Title: Substrates and Inhibitors of SAMHD1
    Article Snippet: Paragraph title: Recombinant SAMHD1-GST purification ... A Millipore Centricon protein concentrator (45 MWCO) was used to concentrate the protein and for buffer exchanges.

    Article Title: The Brucella effector protein TcpB induces degradation of inflammatory caspases and thereby subverts non-canonical inflammasome activation in macrophages
    Article Snippet: Paragraph title: Protein expression and purification ... The eluted protein was concentrated using a Centricon protein concentrator (Millipore) and dialyzed in a buffer containing 50 m m Tris-HCl, 100 m m NaCl, and 10% glycerol.

    Protein Purification:

    Article Title: Active Evasion of CTL Mediated Killing and Low Quality Responding CD8+ T Cells Contribute to Persistence of Brucellosis
    Article Snippet: Paragraph title: Protein purification ... The eluted protein was then subjected to Genenase I protease site to cleave TcpB from MBP followed by SP Sepharose (Sigma) ion exchange chromatography to remove maltose followed by concentration using a centricon protein concentrator (Millipore).

    Plasmid Preparation:

    Article Title: Modulation of microtubule dynamics by a TIR domain protein from the intracellular pathogen Brucella melitensis
    Article Snippet: Briefly, 1 litre of E. coli BL21 cell culture harbouring the pMALTcpB or pMALTcpBG158A plasmid was induced with IPTG (isopropyl β-D-thiogalactopyranoside) to a final concentration of 0.5 mM when the D 600 reached 0.6. .. The eluted protein was then subjected to SP Sepharose (Sigma) ion-exchange chromatography to remove maltose followed by concentration using a Centricon protein concentrator (Millipore).

    Article Title: PI3K/Akt-Dependent Transcriptional Regulation and Activation of BMP-2-Smad Signaling by NF-?B in Metastatic Prostate Cancer Cells
    Article Snippet: The cell culture media were collected after 48 hr and were concentrated in the presence of protease inhibitors at 4°C using Centricon protein concentrator (Millipore, Billerica, MA). .. In another set of experiments, phosphorylation of Smad and Akt proteins was examined in C4-2B cells transduced with 20 MOIs of control vector prior to the addition of rhBMP-2 200 ng/ml for 1 hr.

    Negative Control:

    Article Title: Active Evasion of CTL Mediated Killing and Low Quality Responding CD8+ T Cells Contribute to Persistence of Brucellosis
    Article Snippet: The eluted protein was then subjected to Genenase I protease site to cleave TcpB from MBP followed by SP Sepharose (Sigma) ion exchange chromatography to remove maltose followed by concentration using a centricon protein concentrator (Millipore). .. MBP was used as a negative control.

    Affinity Chromatography:

    Article Title: The Brucella effector protein TcpB induces degradation of inflammatory caspases and thereby subverts non-canonical inflammasome activation in macrophages
    Article Snippet: After the induction, cells were grown at 25 °C for 5 h. Amylose affinity chromatography was used for purification. .. The eluted protein was concentrated using a Centricon protein concentrator (Millipore) and dialyzed in a buffer containing 50 m m Tris-HCl, 100 m m NaCl, and 10% glycerol.

    Concentration Assay:

    Article Title: Modulation of microtubule dynamics by a TIR domain protein from the intracellular pathogen Brucella melitensis
    Article Snippet: .. The eluted protein was then subjected to SP Sepharose (Sigma) ion-exchange chromatography to remove maltose followed by concentration using a Centricon protein concentrator (Millipore). ..

    Article Title: Active Evasion of CTL Mediated Killing and Low Quality Responding CD8+ T Cells Contribute to Persistence of Brucellosis
    Article Snippet: .. The eluted protein was then subjected to Genenase I protease site to cleave TcpB from MBP followed by SP Sepharose (Sigma) ion exchange chromatography to remove maltose followed by concentration using a centricon protein concentrator (Millipore). ..

    Article Title: The Brucella effector protein TcpB induces degradation of inflammatory caspases and thereby subverts non-canonical inflammasome activation in macrophages
    Article Snippet: The eluted protein was concentrated using a Centricon protein concentrator (Millipore) and dialyzed in a buffer containing 50 m m Tris-HCl, 100 m m NaCl, and 10% glycerol. .. The concentration of MBP-TcpB or MBP-TcpB(G158A) was estimated using Bradford reagent (Sigma).

    Lysis:

    Article Title: Substrates and Inhibitors of SAMHD1
    Article Snippet: Cells were harvested and lysed in lysis buffer (50 mM Tris-HCl [pH 7.5], 500 mM NaCl, 2 mM EDTA, 1 mg/ml chicken egg white lysozyme, and one tablet of Roche Applied Science Complete protease inhibitor mixture) for 4 h on ice. .. A Millipore Centricon protein concentrator (45 MWCO) was used to concentrate the protein and for buffer exchanges.

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  • 86
    Millipore dalton mw cutoff
    Dalton Mw Cutoff, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dalton mw cutoff/product/Millipore
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    Millipore centricon protein concentrator
    Centricon Protein Concentrator, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/centricon protein concentrator/product/Millipore
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    centricon protein concentrator - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

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