cellytictm b  (Millipore)


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  • 99
    Name:
    CelLytic B Cell Lysis Reagent
    Description:

    Catalog Number:
    b7435
    Price:
    None
    Applications:
    A proprietary, non-denaturing formulation of zwitterionic detergents used for the lysis of bacterial cells and extraction of recombinant proteins.
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    Structured Review

    Millipore cellytictm b

    https://www.bioz.com/result/cellytictm b/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cellytictm b - by Bioz Stars, 2020-07
    99/100 stars

    Related Products / Commonly Used Together

    bg-11 /
    m-2 s-1
    od750

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    Related Articles

    Centrifugation:

    Article Title: Solid-State Nuclear Magnetic Resonance on the Static and Dynamic Domains of Huntingtin Exon-1 Fibrils
    Article Snippet: .. Cell pellets were collected by centrifugation after 4 hours of incubation and resuspended in 20 mM Tris-HCl, pH 8.0, 300 mM NaCl, and 10 mM imidazole containing 1 × CelLytic B Cell Lysis reagent (Sigma-Aldrich), and incubated for 20 min at room temperature on a shaker. .. Lysates were clarified by centrifugation at 21,000 g for 10 min and incubated with nickel-nitrilotriacetic acid-agarose beads (Qiagen) for 1 h at 4°C on a shaker.

    Article Title: Glutamate biosensor imaging reveals dysregulation of glutamatergic pathways in a model of developmental cortical malformation
    Article Snippet: .. Cells were harvested by centrifugation, resuspended in extraction buffer (50 mM Sodium Phosphate, 300 mM NaCl, pH7.2), disrupted by ultrasonication and further lysed with CelLytic B reagent (Sigma). .. The biosensor protein was purified by Talon His-affinity chromatography (Clontech) by binding to the resin column at 4°C, washing with extraction buffer, and then eluting with extraction buffer containing 150 mM imidazole.

    Article Title: A modified, hypoallergenic variant of the Ricinus communis Ric c1 protein retains biological activity
    Article Snippet: .. The cells were harvested by centrifugation (15 000× g for 10 min), re-suspended in phosphate buffer (50 mM sodium phosphate, pH 8.0; 300 mM NaCl) and lysed by adding CelLytic B, following the instruction manual (Sigma-Aldrich), in the presence of a cocktail of protease inhibitors (Sigma). .. The cell lysate was clarified by centrifugation (15 000× g for 10 min).

    Isolation:

    Article Title: ProTeOn and ProTeOff, new protein devices that inducibly activate bacterial gene expression
    Article Snippet: .. The total soluble protein was isolated from cultures with CelLyticB reagent (B7435 Sigma), separated by size on a 10% polyacrylamide gel and transferred to PVDF membrane. .. PROTEON was detected by primary mouse monoclonal anti-FLAG M2 antibody (F3165 Sigma), GFP by mouse monoclonal anti-GFP antibody [LGB-1] (ab291 Abcam), and loading control RNApol by mouse monoclonal anti-RNApol sigma 70 antibody [2G10] (ab12088).

    Cell Culture:

    Article Title: Genetic and nutrient modulation of acetyl-CoA levels in Synechocystis for n-butanol production
    Article Snippet: .. 14–16 mL cell culture was centrifuged and resuspended in CelLytic B Cell Lysis Reagent (Sigma-Aldrich) supplemented with 1 mM PMSF. .. Acid-washed glass beads, 425–600 μm (Sigma-Aldrich), were added and the cells were lysed by vortexing for 20 min at 4 °C, followed by centrifugation for 4 min at 13,000×g , 4 °C.

    Concentration Assay:

    Article Title: Microgels for Efficient Protein Purification
    Article Snippet: .. The cells were lysed using a 1X concentration of Sigma Cellytic B Cell Lysis reagent, 100 μL of 100 mg mL−1 lysozyme, and ≈250 units of Sigma Benzonase nuclease. .. The lysis solution was spun at 16 000 rpm for 20 min, resuspended in 20 m m Tris (Base), 6 m Urea, and spun again at 16 000 rpm for 20 min.

    Incubation:

    Article Title: Homeostasis of Second Messenger Cyclic-di-AMP Is Critical for Cyanobacterial Fitness and Acclimation to Abiotic Stress
    Article Snippet: .. Cell Lysis Assay The cell lysis assay was conducted by pelleting 7 mL of cells that had been grown in BG-11/HEPES medium under WL at 15 μmol m-2 s-1 as described above and diluted to an OD750 of 0.4 and incubated for an additional 24 h. Pelleted cells were then resuspended in 4 mL of CelLyticTM B (Millipore Sigma, St. Louis, MO, United States) containing 10 μg/mL (w/v) lysozyme and shaken on a vortexer for 30 min. After this period, samples were centrifuged at 10,000 ×g at room temperature for 5 min. ..

    Article Title: The Small Protein HemP Is a Transcriptional Activator for the Hemin Uptake Operon in Burkholderia multivorans ATCC 17616
    Article Snippet: .. After additional incubation for 8 h at 25°C, the cells were collected and disrupted using CelLytic B reagent (Sigma-Aldrich). .. The IPTG-induced HemP or AhpD derivative tagged with six histidine residues at the C terminus was purified using Talon metal affinity resins (TaKaRa), according to the manufacture's protocol.

    Article Title: Solid-State Nuclear Magnetic Resonance on the Static and Dynamic Domains of Huntingtin Exon-1 Fibrils
    Article Snippet: .. Cell pellets were collected by centrifugation after 4 hours of incubation and resuspended in 20 mM Tris-HCl, pH 8.0, 300 mM NaCl, and 10 mM imidazole containing 1 × CelLytic B Cell Lysis reagent (Sigma-Aldrich), and incubated for 20 min at room temperature on a shaker. .. Lysates were clarified by centrifugation at 21,000 g for 10 min and incubated with nickel-nitrilotriacetic acid-agarose beads (Qiagen) for 1 h at 4°C on a shaker.

    Lysis:

    Article Title: Genetic and nutrient modulation of acetyl-CoA levels in Synechocystis for n-butanol production
    Article Snippet: .. 14–16 mL cell culture was centrifuged and resuspended in CelLytic B Cell Lysis Reagent (Sigma-Aldrich) supplemented with 1 mM PMSF. .. Acid-washed glass beads, 425–600 μm (Sigma-Aldrich), were added and the cells were lysed by vortexing for 20 min at 4 °C, followed by centrifugation for 4 min at 13,000×g , 4 °C.

    Article Title: Homeostasis of Second Messenger Cyclic-di-AMP Is Critical for Cyanobacterial Fitness and Acclimation to Abiotic Stress
    Article Snippet: .. Cell Lysis Assay The cell lysis assay was conducted by pelleting 7 mL of cells that had been grown in BG-11/HEPES medium under WL at 15 μmol m-2 s-1 as described above and diluted to an OD750 of 0.4 and incubated for an additional 24 h. Pelleted cells were then resuspended in 4 mL of CelLyticTM B (Millipore Sigma, St. Louis, MO, United States) containing 10 μg/mL (w/v) lysozyme and shaken on a vortexer for 30 min. After this period, samples were centrifuged at 10,000 ×g at room temperature for 5 min. ..

    Article Title: Solid-State Nuclear Magnetic Resonance on the Static and Dynamic Domains of Huntingtin Exon-1 Fibrils
    Article Snippet: .. Cell pellets were collected by centrifugation after 4 hours of incubation and resuspended in 20 mM Tris-HCl, pH 8.0, 300 mM NaCl, and 10 mM imidazole containing 1 × CelLytic B Cell Lysis reagent (Sigma-Aldrich), and incubated for 20 min at room temperature on a shaker. .. Lysates were clarified by centrifugation at 21,000 g for 10 min and incubated with nickel-nitrilotriacetic acid-agarose beads (Qiagen) for 1 h at 4°C on a shaker.

    Article Title: Microgels for Efficient Protein Purification
    Article Snippet: .. The cells were lysed using a 1X concentration of Sigma Cellytic B Cell Lysis reagent, 100 μL of 100 mg mL−1 lysozyme, and ≈250 units of Sigma Benzonase nuclease. .. The lysis solution was spun at 16 000 rpm for 20 min, resuspended in 20 m m Tris (Base), 6 m Urea, and spun again at 16 000 rpm for 20 min.

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  • 99
    Millipore cellytictm b
    Cellytictm B, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cellytictm b/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cellytictm b - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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