cellytic y plus  (Millipore)


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  • 95
    Name:
    CelLytic Y Plus Kit
    Description:

    Catalog Number:
    cyp1
    Price:
    None
    Applications:
    The kit provides a convenient method for a highly efficient spheroplast formation and protein extraction from Saccharomyces cerevisiae, Pichia Pastoris, and Schizosaccharomyces pombe yeast strains. The procedure employs spheroplast formation by enzymatic digestion of the yeast cell wall followed by cell protein extraction (with or without detergent), while avoiding protein degradation and interference with protein immuno­reactivity and biological activity. The cell extract is compatible with:. Reporter gene expression assays (e.g., beta-galactosidase, alkaline-phosphatase) . Immunoassays (Western blots, immunoprecipitation) . Affinity based purification (e.g. FLAG, glutathione S- transferase (GST) and histidine-tagged fusion proteins). DNA-protein interaction assay (e.g., gel-shift). Coomassie and silver stainingThe kit is suitable for spheroplast formation and protein extraction from 10-40 grams of yeast cell pellet.
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    Structured Review

    Millipore cellytic y plus

    https://www.bioz.com/result/cellytic y plus/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cellytic y plus - by Bioz Stars, 2021-01
    95/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Structural basis of interaction between dimeric cyclophilin 1 and Myb1 transcription factor in Trichomonas vaginalis
    Article Snippet: .. Expression and purification of Tv CyP1 and Tv CyP1 mutants Tv CyP1 gene was cloned into pET-28a vector (Novagen) and was expressed with an N-terminal His-tag followed by a TEV digestion recognition site in the Escherichia coli strain BL21(DE3). ..

    Flow Cytometry:

    Article Title: Structural basis of interaction between dimeric cyclophilin 1 and Myb1 transcription factor in Trichomonas vaginalis
    Article Snippet: .. The flow-through containing Tv CyP1 was buffer-exchanged in 20 mM NaH2 PO4 , 50 mM NaCl and 0.5 mM NaN3 (pH 6.0) by centrifugation with 10,000 Da MWC membrane ultrafiltration (Millipore). ..

    Positron Emission Tomography:

    Article Title: Structural basis of interaction between dimeric cyclophilin 1 and Myb1 transcription factor in Trichomonas vaginalis
    Article Snippet: .. Expression and purification of Tv CyP1 and Tv CyP1 mutants Tv CyP1 gene was cloned into pET-28a vector (Novagen) and was expressed with an N-terminal His-tag followed by a TEV digestion recognition site in the Escherichia coli strain BL21(DE3). ..

    Fluorescence:

    Article Title: Analysis of the AHR gene proximal promoter GGGGC-repeat polymorphism in lung, breast, and colon cancer
    Article Snippet: .. In brief, cells in their respective media were treated with 10 nM TCDD (Cambridge Isotope Laboratories, Andover, MA) or 0.1% dimethyl sulfoxide (DMSO) vehicle for 48 h. Conversion of the CYP1 substrate, ethoxyresorufin (Sigma), to resorufin (Sigma) was monitored after 30 min by measuring fluorescence at 590 nm elicited by excitation at 535 nm. .. EROD activities were normalized to total protein content as measured using the BCA protein assay (Thermo Fisher).

    Synthesized:

    Article Title: Induction patterns of new CYP1 genes in environmentally exposed rainbow trout
    Article Snippet: .. Amplification of cDNA was performed using the Advantage® 2 Polymerase PCR Kit (Clontech Laboratories Inc., Mountain View, CA, USA) with salmon and rainbow trout CYP1 gene-specific primers synthesized by Sigma-Aldrich (St. Louis, MO, USA) and Eurofins MWG GmbH, (Ebersberg, Germany). .. For determination of the mRNA 3’ and 5’ends the SMART™ RACE cDNA Amplification Kit (Clontech Laboratories Inc) was used.

    Centrifugation:

    Article Title: Structural basis of interaction between dimeric cyclophilin 1 and Myb1 transcription factor in Trichomonas vaginalis
    Article Snippet: .. The flow-through containing Tv CyP1 was buffer-exchanged in 20 mM NaH2 PO4 , 50 mM NaCl and 0.5 mM NaN3 (pH 6.0) by centrifugation with 10,000 Da MWC membrane ultrafiltration (Millipore). ..

    Purification:

    Article Title: Structural basis of interaction between dimeric cyclophilin 1 and Myb1 transcription factor in Trichomonas vaginalis
    Article Snippet: .. Expression and purification of Tv CyP1 and Tv CyP1 mutants Tv CyP1 gene was cloned into pET-28a vector (Novagen) and was expressed with an N-terminal His-tag followed by a TEV digestion recognition site in the Escherichia coli strain BL21(DE3). ..

    Amplification:

    Article Title: Induction patterns of new CYP1 genes in environmentally exposed rainbow trout
    Article Snippet: .. Amplification of cDNA was performed using the Advantage® 2 Polymerase PCR Kit (Clontech Laboratories Inc., Mountain View, CA, USA) with salmon and rainbow trout CYP1 gene-specific primers synthesized by Sigma-Aldrich (St. Louis, MO, USA) and Eurofins MWG GmbH, (Ebersberg, Germany). .. For determination of the mRNA 3’ and 5’ends the SMART™ RACE cDNA Amplification Kit (Clontech Laboratories Inc) was used.

    Activity Assay:

    Article Title: Expression Profile of CYP1A1 and CYP1B1 Enzymes in Colon and Bladder Tumors
    Article Snippet: .. To further substantiate that CYP1A1 is a minor contributor to CYP1 activity noted in tumors we performed incubations of tumor microsomes with diosmetin in the presence of a polyclonal antibody raised against human CYP1A1 purchased from Millipore (AB 1258, Massachusetts, US). .. Our analysis showed that co-incubation of CYP1A1 antibody reduced the activity albeit to levels that were lower to those observed in the case of the CYP1B1 antibody ( ).

    Expressing:

    Article Title: Expression of Pneumocystis jirovecii Major Surface Glycoprotein in Saccharomyces cerevisiae
    Article Snippet: .. Yeast cells expressing Msg on the surface were treated with β-1,3 glucanase (lyticase, CelLytic Y Plus Kit, Sigma-Aldrich) overnight at 37°C. .. The supernatant obtained was analyzed by Western blot, and the pellet was used for immunofluorescence analysis.

    Article Title: Structural basis of interaction between dimeric cyclophilin 1 and Myb1 transcription factor in Trichomonas vaginalis
    Article Snippet: .. Expression and purification of Tv CyP1 and Tv CyP1 mutants Tv CyP1 gene was cloned into pET-28a vector (Novagen) and was expressed with an N-terminal His-tag followed by a TEV digestion recognition site in the Escherichia coli strain BL21(DE3). ..

    Polymerase Chain Reaction:

    Article Title: Induction patterns of new CYP1 genes in environmentally exposed rainbow trout
    Article Snippet: .. Amplification of cDNA was performed using the Advantage® 2 Polymerase PCR Kit (Clontech Laboratories Inc., Mountain View, CA, USA) with salmon and rainbow trout CYP1 gene-specific primers synthesized by Sigma-Aldrich (St. Louis, MO, USA) and Eurofins MWG GmbH, (Ebersberg, Germany). .. For determination of the mRNA 3’ and 5’ends the SMART™ RACE cDNA Amplification Kit (Clontech Laboratories Inc) was used.

    Plasmid Preparation:

    Article Title: Structural basis of interaction between dimeric cyclophilin 1 and Myb1 transcription factor in Trichomonas vaginalis
    Article Snippet: .. Expression and purification of Tv CyP1 and Tv CyP1 mutants Tv CyP1 gene was cloned into pET-28a vector (Novagen) and was expressed with an N-terminal His-tag followed by a TEV digestion recognition site in the Escherichia coli strain BL21(DE3). ..

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  • 99
    Millipore mlb buffer
    Mlb Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mlb buffer/product/Millipore
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    mlb buffer - by Bioz Stars, 2021-01
    99/100 stars
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