cellulase  (Gold Biotechnology Inc)

Journal: Scientific Reports

Article Title: Combinatorial optimization of the hybrid cellulase complex structure designed from modular libraries

doi: 10.1038/s41598-024-73541-2

Figure Lengend Snippet: Hybrid cellulase complex design in this study.

Article Snippet: Recently, we designed a novel artificial hybrid cellulase complex by using biotinylated CD (CD of endoglucanases) and CBD prepared separately and then reassembled in vitro on the surface of streptavidin-conjugated CdSe nanoparticles (quantum dot) via a biotin-avidin interaction – .

Techniques:

Journal: Scientific Reports

Article Title: Combinatorial optimization of the hybrid cellulase complex structure designed from modular libraries

doi: 10.1038/s41598-024-73541-2

Figure Lengend Snippet: Producing reducing sugar from phosphoric-acid swollen cellulose (PSC, 3.5 mg mL –1 ) in a 50 mM acetate buffer (pH 5.0, 200 mM NaCl) at 45 °C for 96 h in the presence of free CD, a CD and CBD mixture (CD + CBD), and a native modular enzymes (native) CD and CBD cluster on streptavidin-conjugated CdSe nanoparticles (hybrid cellulase complex), made from five module enzymes, ( a ) CD 5-6 , ( b ) CD 6-4 , ( c ) CD 7 , ( d ) CD 26 , and ( e ) CD 44 . Native CBDs used in the experiments were CBD 1-4 , CBD 2-3 , CBD 1-5 , CBD 11 and CBD 44 , respectively. All experiments were performed at a CD concentration of 40 nM.

Article Snippet: Recently, we designed a novel artificial hybrid cellulase complex by using biotinylated CD (CD of endoglucanases) and CBD prepared separately and then reassembled in vitro on the surface of streptavidin-conjugated CdSe nanoparticles (quantum dot) via a biotin-avidin interaction – .

Techniques: Concentration Assay

Journal: Scientific Reports

Article Title: Combinatorial optimization of the hybrid cellulase complex structure designed from modular libraries

doi: 10.1038/s41598-024-73541-2

Figure Lengend Snippet: Thermal stability of clustered enzymes (normal line) and free (dotted line) at 50 °C (blue) and 70 °C (red). The enzymes (2.5 μM) were maintained at both temperatures without the substrate for 3 days in acetate buffer at pH 5.0. The activity determined before incubation was at 100%. Produced reducing sugars were measured at an enzyme concentration of 40 nM. The actual reducing sugar concentrations produced by these hybrid cellulase complex solutions at 0 h were 0.57 mg ml-1 at 50 ℃ at without nanoparticles, 1.96 mg ml-1 at 50 ℃ with nanoparticles, 0.55 mg ml-1 at 70 ℃ without nanoparticles, and 1.87 mg ml-1 at 70 ℃ with nanoparticles.

Article Snippet: Recently, we designed a novel artificial hybrid cellulase complex by using biotinylated CD (CD of endoglucanases) and CBD prepared separately and then reassembled in vitro on the surface of streptavidin-conjugated CdSe nanoparticles (quantum dot) via a biotin-avidin interaction – .

Techniques: Activity Assay, Incubation, Produced, Concentration Assay