cellotetraose  (Millipore)


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    Name:
    Cellotetraose
    Description:

    Catalog Number:
    c8286
    Price:
    None
    Applications:
    Cellotetraose is a 4-glucose polymer cellulodextrin derived from cellulose degradation. Cellotetraose and other cellulodextrins, such as cellulobiose, cellulotriose and cellopentaose are used by cellulosic bacteria as sources of energy. Cellotetraose is used to help identify, differentiate and characterize oligosaccharide metabolizing enzymes.
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    Structured Review

    Millipore cellotetraose
    Cellotetraose

    https://www.bioz.com/result/cellotetraose/product/Millipore
    Average 96 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    cellotetraose - by Bioz Stars, 2020-09
    96/100 stars

    Images

    1) Product Images from "Combining free and aggregated cellulolytic systems in the cellulosome-producing bacterium Ruminiclostridium cellulolyticum"

    Article Title: Combining free and aggregated cellulolytic systems in the cellulosome-producing bacterium Ruminiclostridium cellulolyticum

    Journal: Biotechnology for Biofuels

    doi: 10.1186/s13068-015-0301-4

    In vitro combination of Cel9A and purified cellulosomes on Avicel 3.5 g/L. 9A designates purified Cel9A (from E. coli ) at 10.5 mg/L. Cell. corresponds to purified cellulosomes from R. cellulolyticum carrying p0 by gel filtration at 10.5 mg/L. Cell. + 9A designates a mixture of 10.5 mg/L of Cel9A and 10.5 mg/L of purified cellulosomes. The soluble sugars released after 1, 6 and 24 h of incubation at 37°C (incubation time indicated at the bottom) were identified and quantified by HPAEC-PAD. G1, G2, G3 and G4 designate glucose, cellobiose, cellotriose and cellotetraose, respectively. The data show the mean of two independent experiments and the bars indicate the standard deviation.
    Figure Legend Snippet: In vitro combination of Cel9A and purified cellulosomes on Avicel 3.5 g/L. 9A designates purified Cel9A (from E. coli ) at 10.5 mg/L. Cell. corresponds to purified cellulosomes from R. cellulolyticum carrying p0 by gel filtration at 10.5 mg/L. Cell. + 9A designates a mixture of 10.5 mg/L of Cel9A and 10.5 mg/L of purified cellulosomes. The soluble sugars released after 1, 6 and 24 h of incubation at 37°C (incubation time indicated at the bottom) were identified and quantified by HPAEC-PAD. G1, G2, G3 and G4 designate glucose, cellobiose, cellotriose and cellotetraose, respectively. The data show the mean of two independent experiments and the bars indicate the standard deviation.

    Techniques Used: In Vitro, Purification, Filtration, Incubation, Standard Deviation

    Avicelase activity of the purified cellulosomes produced by the various recombinant R. cellulolyticum strains. The purified cellulosomes were assayed at 8 mg/L on 3.5 g/L microcrystalline cellulose for 24 h at 37°C. Released soluble sugars were identified and quantified by HPAEC-PAD. The recombinant strains carrying either p0, pCel9A or pCel9Ac are indicated at the bottom of each bar. G1, G2, G3 and G4 designate glucose, cellobiose, cellotriose and cellotetraose, respectively. The data show the mean of three independent experiments and the bars indicate the standard deviation.
    Figure Legend Snippet: Avicelase activity of the purified cellulosomes produced by the various recombinant R. cellulolyticum strains. The purified cellulosomes were assayed at 8 mg/L on 3.5 g/L microcrystalline cellulose for 24 h at 37°C. Released soluble sugars were identified and quantified by HPAEC-PAD. The recombinant strains carrying either p0, pCel9A or pCel9Ac are indicated at the bottom of each bar. G1, G2, G3 and G4 designate glucose, cellobiose, cellotriose and cellotetraose, respectively. The data show the mean of three independent experiments and the bars indicate the standard deviation.

    Techniques Used: Activity Assay, Purification, Produced, Recombinant, Standard Deviation

    Activity on microcrystalline cellulose of bi-functional hybrid cellulosomes containing Cel9At and Cel48F. The amount of released soluble cellodextrins and their proportion by 0.1 µM of complexes or Cel9At and Cel48F bound to the corresponding free cohesins after 24 h of incubation at 37°C with 3.5 g/L Avicel are shown. Soluble sugars were identified and quantified by HPAEC-PAD. G1, G2, G3 and G4 designate glucose, cellobiose, cellotriose and cellotetraose, respectively. The data show the mean of three independent experiments and bars indicate the standard deviation.
    Figure Legend Snippet: Activity on microcrystalline cellulose of bi-functional hybrid cellulosomes containing Cel9At and Cel48F. The amount of released soluble cellodextrins and their proportion by 0.1 µM of complexes or Cel9At and Cel48F bound to the corresponding free cohesins after 24 h of incubation at 37°C with 3.5 g/L Avicel are shown. Soluble sugars were identified and quantified by HPAEC-PAD. G1, G2, G3 and G4 designate glucose, cellobiose, cellotriose and cellotetraose, respectively. The data show the mean of three independent experiments and bars indicate the standard deviation.

    Techniques Used: Activity Assay, Functional Assay, Incubation, Standard Deviation

    2) Product Images from "Synergistic Hydrolysis of Carboxymethyl Cellulose and Acid-Swollen Cellulose by Two Endoglucanases (CelZ and CelY) from Erwinia chrysanthemi †"

    Article Title: Synergistic Hydrolysis of Carboxymethyl Cellulose and Acid-Swollen Cellulose by Two Endoglucanases (CelZ and CelY) from Erwinia chrysanthemi †

    Journal: Journal of Bacteriology

    doi:

    Thin-layer chromatography analysis of the hydrolysis products from acid-swollen cellulose and Avicel. Abbreviations for y axis: G1, glucose; G2, cellobiose; G3, cellotriose; G4, cellotetraose; and G5, cellopentaose. Lanes: S, mixed-cellooligosaccharide standard; C, control lacking enzyme; Z, CelZ; Y, CelY; and Y + Z, CelY plus CelZ. (A) Acid-swollen cellulose (6-h incubation, 1-μl loading); (B) acid-swollen cellulose (6-h incubation, 2-μl loading); (C) Avicel (48-h incubation, 10-μl loading).
    Figure Legend Snippet: Thin-layer chromatography analysis of the hydrolysis products from acid-swollen cellulose and Avicel. Abbreviations for y axis: G1, glucose; G2, cellobiose; G3, cellotriose; G4, cellotetraose; and G5, cellopentaose. Lanes: S, mixed-cellooligosaccharide standard; C, control lacking enzyme; Z, CelZ; Y, CelY; and Y + Z, CelY plus CelZ. (A) Acid-swollen cellulose (6-h incubation, 1-μl loading); (B) acid-swollen cellulose (6-h incubation, 2-μl loading); (C) Avicel (48-h incubation, 10-μl loading).

    Techniques Used: Thin Layer Chromatography, Incubation

    Hydrolysis of cellooligosaccharides by CelZ and CelY. Each test contained approximately 0.07 IU of CMCase per ml (2-h incubation, 35°C). Abbreviations: S, mixed-cellooligosaccharide standard; G1, glucose; G2, cellobiose; G3, cellotriose; G4, cellotetraose; and G5, cellopentaose. (A) Before hydrolysis; (B) after incubation with CelY; (C) after incubation with CelZ; (D) CelZ hydrolysis of cellopentaose after different periods of incubation (0, 5, 10, and 25 min).
    Figure Legend Snippet: Hydrolysis of cellooligosaccharides by CelZ and CelY. Each test contained approximately 0.07 IU of CMCase per ml (2-h incubation, 35°C). Abbreviations: S, mixed-cellooligosaccharide standard; G1, glucose; G2, cellobiose; G3, cellotriose; G4, cellotetraose; and G5, cellopentaose. (A) Before hydrolysis; (B) after incubation with CelY; (C) after incubation with CelZ; (D) CelZ hydrolysis of cellopentaose after different periods of incubation (0, 5, 10, and 25 min).

    Techniques Used: Incubation

    Model illustrating the utilization of amorphous cellulose by E. chrysanthemi . Three glucosidases are used for the catabolism of amorphous cellulose. Two of these, CelY and CelZ, are extracellular endoglucanases which function together in a synergistic fashion. CelY requires large substrate molecules and hydrolyzes these into shorter, insoluble fragments. CelY does not hydrolyze soluble cellooligosaccharides (2 to 5 glucosyl residues). CelZ readily hydrolyzes soluble cellooligosaccharides (cellopentaose and cellotetraose) and amorphous fragments of intermediate lengths to produce cellobiose and cellotriose. Cellobiose (G-G) and cellotriose (G-G-G) are phosphorylated (P) during cellular uptake by a phosphoenolpyruvate-dependent phosphotransferase system (PTS). Hydrolysis is completed intracellularly by a third enzyme, phospho-β-glucosidase. Resulting monomeric products (glucose and glucose-6-phosphate) are metabolized by glycolysis.
    Figure Legend Snippet: Model illustrating the utilization of amorphous cellulose by E. chrysanthemi . Three glucosidases are used for the catabolism of amorphous cellulose. Two of these, CelY and CelZ, are extracellular endoglucanases which function together in a synergistic fashion. CelY requires large substrate molecules and hydrolyzes these into shorter, insoluble fragments. CelY does not hydrolyze soluble cellooligosaccharides (2 to 5 glucosyl residues). CelZ readily hydrolyzes soluble cellooligosaccharides (cellopentaose and cellotetraose) and amorphous fragments of intermediate lengths to produce cellobiose and cellotriose. Cellobiose (G-G) and cellotriose (G-G-G) are phosphorylated (P) during cellular uptake by a phosphoenolpyruvate-dependent phosphotransferase system (PTS). Hydrolysis is completed intracellularly by a third enzyme, phospho-β-glucosidase. Resulting monomeric products (glucose and glucose-6-phosphate) are metabolized by glycolysis.

    Techniques Used:

    Related Articles

    Co-Elution Assay:

    Article Title: Insoluble (1 → 3), (1 → 4)-β-D-glucan is a component of cell walls in brown algae (Phaeophyceae) and is masked by alginates in tissues
    Article Snippet: .. In order to eliminate possible co-elution between the MLG trisaccharide, cellotriose or maltotriose, appropriate controls consisting of MLG (Glucagel) (DKSH, Italy), maltotriose, maltotetraose, cellotriose, cellotetraose (all Sigma, USA) were treated as described above. .. No co-elution could be observed between the MLG trisaccharide and any of the standards (Supplementary Information Fig. ).

    other:

    Article Title: Synergistic Hydrolysis of Carboxymethyl Cellulose and Acid-Swollen Cellulose by Two Endoglucanases (CelZ and CelY) from Erwinia chrysanthemi †
    Article Snippet: Antibiotics, low-viscosity CMC, cellobiose, cellotriose, and cellotetraose were obtained from the Sigma Chemical Co. (St. Louis, Mo.).

    Glucose Assay:

    Article Title: The putative β-glucosidase BGL3I regulates cellulase induction in Trichoderma reesei
    Article Snippet: .. The specific activities of β-glucosidases to cellobiose (G2: Sangon, Shanghai, China), cellotriose (G3: Sigma, Saint Louis, MO, USA), cellotetraose (G4: Sigma, Saint Louis, MO, USA), cellopentaose (G5: Sigma, Saint Louis, MO, USA), lactose (Sinopharm, Shanghai, China), and sophorose (Sigma, Saint Louis, MO, USA) were assayed using the Glucose Assay Kit (Sigma, Saint Louis, MO, USA). .. Mycelia were suspended in 50 mM sodium acetate buffer (pH 7.0) with protease inhibitor and homogenized by the FastPrep® -24 (MP Biomedicals, Solon, HO, USA) instrument.

    Injection:

    Article Title: Microglia are Indispensable for Synaptic Plasticity in the Spinal Dorsal Horn and Chronic Pain
    Article Snippet: .. Then two doses of diphtheria toxin (DT, Sigma, C8286, 50 μg/kg, 2.5 μg/ml in PBS) or vehicle (corn oil) were i.p. injected 3 weeks later to induce microglial ablation. .. To test the role of microglial ablation or repopulation or the effect of TM only, HFS-induced synaptic plasticity and chronic pain, HFS was delivered to the sciatic nerve on the 3rd or 21st day after the first DT injection, or the 7th day before the first DT injection, respectively.

    Article Title: Combining free and aggregated cellulolytic systems in the cellulosome-producing bacterium Ruminiclostridium cellulolyticum
    Article Snippet: .. Injection of samples containing glucose, cellobiose, cellotriose, cellotetraose, cellopentaose (Sigma-Aldrich) and cellohexaose (Seikagaku, Tokyo, Japan) at known concentrations (ranging from 5 to 100 µM) was use d to identify and quantify the released sugars. ..

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  • 96
    Millipore cellotetraose
    Cellotetraose, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cellotetraose/product/Millipore
    Average 96 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    cellotetraose - by Bioz Stars, 2020-09
    96/100 stars
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