cell impermeable sulfosuccinimidyl 6  (Thermo Fisher)


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    Name:
    EZ Link Sulfo NHS SS Biotin
    Description:
    Thermo Scientific EZ Link Sulfo NHS SS Biotin is a water soluble NHS ester biotinylation reagent whose spacer arm includes a cleavable disulfide bond for reversible labeling of proteins and cell surface primary amines Features of EZ Link Sulfo NHS SS Biotin • Protein labeling biotinylate antibodies to facilitate immobilization purification or detection • Cell surface labeling biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes • Amine reactive reacts with primary amines NH2 such as lysine side chains or the amino termini of polypeptides • Cleavable disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT only a small sulfhydryl group remains attached to the molecule • Soluble charged sulfo NHS group increases reagent water solubility compared to ordinary NHS ester compounds • Medium length spacer arm total length added to target is 24 3 angstroms it consists of the native biotin valeric acid group extended by a 7 atom chain Sulfo NHS SS Biotin is a thiol cleavable amine reactive biotinylation reagent that contains an extended spacer arm to reduce steric hindrances associated with avidin binding This reagent is water soluble enabling biotinylation to be performed in the absence of organic solvents such as DMSO or DMF for applications that cannot tolerate solvents or are complicated by their inclusion The compound is particularly useful for labeling and purifying cell surface proteins because a its sulfonate group prevents it from permeating cell membranes and b its cleavable spacer arm enables initially biotinylated proteins to be released from streptavidin affinity columns We manufacture biotin reagents to ensure the highest possible overall product integrity consistency and performance for the intended research applications N Hydroxysulfosuccinimide NHS esters of biotin are the most popular type of biotinylation reagent NHS activated biotins react efficiently with primary amino groups NH2 in alkaline buffers to form stable amide bonds Proteins e g antibodies typically have several primary amines that are available as targets for labeling including the side chain of lysine K residues and the N terminus of each polypeptide Varieties of biotin NHS ester reagents differ in length solubility cell permeability and cleavability Non sulfonated NHS biotins are cell permeable but must be dissolved in organic solvent such as DMSO or DMF Sulfo NHS biotins and those with pegylated spacers are directly water soluble but not membrane permeable Varieties containing disulfide bonds can be cleaved using reducing agents enabling the biotin group to be disconnected from the labeled protein Related Products EZ Link Sulfo NHS SS Biotinylation Kit EZ Link Micro Sulfo NHS SS Biotinylation Kit Pierce Premium Grade Sulfo NHS SS Biotin
    Catalog Number:
    21331
    Price:
    None
    Applications:
    Protein Biology|Protein Labeling|Protein Labeling & Crosslinking
    Category:
    Labeling Detection Products
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    Structured Review

    Thermo Fisher cell impermeable sulfosuccinimidyl 6
    Thermo Scientific EZ Link Sulfo NHS SS Biotin is a water soluble NHS ester biotinylation reagent whose spacer arm includes a cleavable disulfide bond for reversible labeling of proteins and cell surface primary amines Features of EZ Link Sulfo NHS SS Biotin • Protein labeling biotinylate antibodies to facilitate immobilization purification or detection • Cell surface labeling biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes • Amine reactive reacts with primary amines NH2 such as lysine side chains or the amino termini of polypeptides • Cleavable disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT only a small sulfhydryl group remains attached to the molecule • Soluble charged sulfo NHS group increases reagent water solubility compared to ordinary NHS ester compounds • Medium length spacer arm total length added to target is 24 3 angstroms it consists of the native biotin valeric acid group extended by a 7 atom chain Sulfo NHS SS Biotin is a thiol cleavable amine reactive biotinylation reagent that contains an extended spacer arm to reduce steric hindrances associated with avidin binding This reagent is water soluble enabling biotinylation to be performed in the absence of organic solvents such as DMSO or DMF for applications that cannot tolerate solvents or are complicated by their inclusion The compound is particularly useful for labeling and purifying cell surface proteins because a its sulfonate group prevents it from permeating cell membranes and b its cleavable spacer arm enables initially biotinylated proteins to be released from streptavidin affinity columns We manufacture biotin reagents to ensure the highest possible overall product integrity consistency and performance for the intended research applications N Hydroxysulfosuccinimide NHS esters of biotin are the most popular type of biotinylation reagent NHS activated biotins react efficiently with primary amino groups NH2 in alkaline buffers to form stable amide bonds Proteins e g antibodies typically have several primary amines that are available as targets for labeling including the side chain of lysine K residues and the N terminus of each polypeptide Varieties of biotin NHS ester reagents differ in length solubility cell permeability and cleavability Non sulfonated NHS biotins are cell permeable but must be dissolved in organic solvent such as DMSO or DMF Sulfo NHS biotins and those with pegylated spacers are directly water soluble but not membrane permeable Varieties containing disulfide bonds can be cleaved using reducing agents enabling the biotin group to be disconnected from the labeled protein Related Products EZ Link Sulfo NHS SS Biotinylation Kit EZ Link Micro Sulfo NHS SS Biotinylation Kit Pierce Premium Grade Sulfo NHS SS Biotin
    https://www.bioz.com/result/cell impermeable sulfosuccinimidyl 6/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cell impermeable sulfosuccinimidyl 6 - by Bioz Stars, 2020-10
    99/100 stars

    Related Products / Commonly Used Together

    biotinylation

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    Related Articles

    Transfection:

    Article Title: Effect of C-Terminal S-Palmitoylation on D2 Dopamine Receptor Trafficking and Stability
    Article Snippet: .. Cell surface biotinylation assay HEK-293T cells transfected with FLAG-tagged WT-D2R or ΔC443-D2R, or HEK-D2L cells treated with DMSO or 15-HDYA were washed with PBS and treated with 300 μg/mL EZ-Link Sulfo-NHS-SS-Biotin (Thermo Scientific, Rockford, IL) in PBS for 30 minutes at 4°C. .. Biotin solution was removed, cells were washed with 50 mM Tris, pH 7.4,150 mM NaCl and lysed in sodium phosphate lysis buffer as described above.

    Labeling:

    Article Title: Detection of Active Matriptase Using a Biotinylated Chloromethyl Ketone Peptide
    Article Snippet: .. For labeling of surface proteins, cells were biotinylated from the basolateral side with 1 mg/ml EZ-link™ Sulfo-NHS-SS-Biotin (Pierce) dissolved in PBS++ for 30 min at 4°C as previously described . ..

    Cell Surface Biotinylation Assay:

    Article Title: Effect of C-Terminal S-Palmitoylation on D2 Dopamine Receptor Trafficking and Stability
    Article Snippet: .. Cell surface biotinylation assay HEK-293T cells transfected with FLAG-tagged WT-D2R or ΔC443-D2R, or HEK-D2L cells treated with DMSO or 15-HDYA were washed with PBS and treated with 300 μg/mL EZ-Link Sulfo-NHS-SS-Biotin (Thermo Scientific, Rockford, IL) in PBS for 30 minutes at 4°C. .. Biotin solution was removed, cells were washed with 50 mM Tris, pH 7.4,150 mM NaCl and lysed in sodium phosphate lysis buffer as described above.

    Incubation:

    Article Title: Alteration of EGFR Spatiotemporal Dynamics Suppresses Signal Transduction
    Article Snippet: .. Biotinylation To assess cell surface EGFR and receptor internalization, cells were treated with fatty acids, incubated with low serum media and surface biotinylated using thiol-cleavable EZ-Link Sulfo-NHS-SS-Biotin (Pierce), 0.5 mg/mL, dissolved in PBS for 30 min on ice. .. Labeled cells were then rinsed two times with ice-cold PBS, and excess biotin was quenched with 60 mM iodoacetamide in PBS buffer for 5 min at 4°C.

    Article Title: ZIP8 Is an Iron and Zinc Transporter Whose Cell-surface Expression Is Up-regulated by Cellular Iron Loading *
    Article Snippet: .. Briefly, H4IIE cells were incubated with EZ-Link Sulfo-NHS-SS-Biotin to biotinylate cell-surface proteins, which were subsequently affinity-purified by using NeutrAvidin-agarose resin (Thermo Scientific). .. Bound proteins were released by incubating with SDS-PAGE buffer containing 50 m m DTT.

    other:

    Article Title: ?-AP-2 Directs Myosin VI-dependent Endocytosis of Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channels in the Intestine *
    Article Snippet: EZ-link sulfo-NHS-SS-biotin and immunopure immobilized streptavidin-agarose beads were obtained from Pierce.

    Article Title: Clathrin-independent carriers form a high capacity endocytic sorting system at the leading edge of migrating cells
    Article Snippet: CTxB biotinylation CTxB-555 was biotinylated with 2 mg.ml−1 EZ-link Sulfo-NHS-SS-biotin (Thermo Fisher Scientific) for 10 min at 37°C and biotin quenched with 1:1 volume of 200 mM glycine for 5 min. CTxB-biotin was added to Cav1−/− MEFs either treated with 30 µM Dyngo4a or left untreated for the times indicated.

    Affinity Purification:

    Article Title: ZIP8 Is an Iron and Zinc Transporter Whose Cell-surface Expression Is Up-regulated by Cellular Iron Loading *
    Article Snippet: .. Briefly, H4IIE cells were incubated with EZ-Link Sulfo-NHS-SS-Biotin to biotinylate cell-surface proteins, which were subsequently affinity-purified by using NeutrAvidin-agarose resin (Thermo Scientific). .. Bound proteins were released by incubating with SDS-PAGE buffer containing 50 m m DTT.

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  • 99
    Thermo Fisher cell impermeable sulfosuccinimidyl 6
    Cell Impermeable Sulfosuccinimidyl 6, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell impermeable sulfosuccinimidyl 6/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cell impermeable sulfosuccinimidyl 6 - by Bioz Stars, 2020-10
    99/100 stars
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