cd8 memory 130 094 412 t cells  (Miltenyi Biotec)

Journal: bioRxiv

Article Title: Distinct mechanisms are employed by T-cell-instructed myeloid cells for IL-1β production in humans and mice

doi: 10.1101/2025.02.26.640341

Figure Lengend Snippet: Isolated monocytes, autologous memory CD4+ T-cells or memory CD8+ T-cells from human PBMCs were cultured alone or co-cultured in the absence/presence of anti-CD3 antibodies for 16 hours. Immunoblotting analysis of pro-IL-1β, β-actin, and cleaved-IL-1β in the whole cell lysates or the supernatants.

Article Snippet: Primary human monocytes, memory CD8+ T cells and memory CD4+ T cells were isolated from PBMCs using CD14 MicroBeadsTM, memory CD8+ isolation kit and memory CD4+ isolation kit according to the manufacturer’s instructions (Miltenyi Biotec), respectively.

Techniques: Isolation, Cell Culture, Western Blot

Journal: STAR Protocols

Article Title: Protocol for assessing T cell receptor-mediated human T cell cytotoxicity

doi: 10.1016/j.xpro.2024.103541

Figure Lengend Snippet: Results of cytotoxicity assays using CD8 T cells following various stimulations (A) Comparison of cell death of L cells as measured by Annexin V following assay with unstimulated and stimulated CD8 T cells in the presence and absence of anti-CD3-incubated L cells. ○ depicts total CD8 T cells, □ depicts memory CD8 T cells. n = 6 (B) Comparison of cell death of L cells pre-incubated with anti-CD3 OKT3 clone vs. SK7 clone following incubation with CD8 T cells. n = 3 (C) Comparison of cell death by CD8+ T cells unstimulated or stimulated with IL15 or IFNβ in L cells pre-incubated with anti-CD3 OKT3 clone vs. SK7 clone. ∗∗ p ≤ 0.01, ∗ p ≤ 0.05 by ratio paired T test. n = 3 (D) Annexin V staining in L cells pre-incubated with anti-CD3 after incubation with unstimulated or anti-CD3/anti-CD28 stimulated naive (∗∗ p ≤ 0.01), memory (∗∗ p ≤ 0.01) or TEMRA CD8+ T cells. n = 4 (E) Annexin V staining of L cells after incubation with unstimulated or IFNβ stimulated naïve, memory, or TEMRA (∗ p ≤ 0.05) CD8 T cells. n = 5 (F) Memory CD8+ T cells cultured in complete media for 3 days in the presence and absence of anti-CD3/anti-CD28 were exposed to V-type H + -ATPase inhibitor concanamycin A (CMA) at 10 nM, then incubated with L cells pre-incubated with anti-CD3 antibody. Cytotoxicity of memory CD8+ T cells was inhibited by exposure to concanamycin A. n = 6. ○ depicts usage of SK7, □ depicts usage of OKT3 during assay. Each color represents a different healthy T cell donor.

Article Snippet: CD8+ Memory T Cell Isolation Kit, human , Miltenyi Biotec , 130-094-412.

Techniques: Comparison, Incubation, Staining, Cell Culture

Journal: STAR Protocols

Article Title: Protocol for assessing T cell receptor-mediated human T cell cytotoxicity

doi: 10.1016/j.xpro.2024.103541

Figure Lengend Snippet:

Article Snippet: CD8+ Memory T Cell Isolation Kit, human , Miltenyi Biotec , 130-094-412.

Techniques: Binding Assay, Recombinant, Cell Isolation, Expressing, Software, Sterility