Journal: Advanced Healthcare Materials

Article Title: Genetically Engineered Macrophages Co‐Loaded with CD47 Inhibitors Synergistically Reconstruct Efferocytosis and Improve Cardiac Remodeling Post Myocardial Ischemia Reperfusion Injury

doi: 10.1002/adhm.202303267

Figure Lengend Snippet: Schematic illustration of MAC CCR2+MerTK CR ‐Lipo PEP‐20 as a potential candidate to reconstruct efferocytosis post‐MI/R injury. A) Fabrication of MAC CCR2+MerTK CR ‐Lipo PEP‐20 . B) After intravenous injection, MAC CCR2+MerTK CR ‐Lipo PEP‐20 selectively migrates to the site of cardiac injury through the overexpressed CCR2. The overexpressed MerTK CR on MAC CCR2+MerTK CR ‐Lipo PEP‐20 remains intact and recognizes phosphatidylserine through Gas6 (growth arrest specific 6) or ProS (protein S) bridging, thereby executing efferocytosis function. The anchored PEP‐20 on MAC CCR2+MerTK CR ‐Lipo PEP‐20 is explosively released in response to ROS stimulation, which further enhances the efferocytosis capacity of adoptive macrophages and resident macrophages by antagonizing CD47 on apoptotic cells. C) The cardiac benefits of improved efferocytosis are achieved through inhibiting initiation and promoting active resolution of inflammation.

Article Snippet: FITC‐labeled CD47 protein (CD7‐HF3H3, ACRO Biosystems, China) or isotype control (BSA‐FITC, WH0092607, Weihua Bio, China) was used for the detection of PEP‐20.

Techniques: Injection

Journal: Advanced Healthcare Materials

Article Title: Genetically Engineered Macrophages Co‐Loaded with CD47 Inhibitors Synergistically Reconstruct Efferocytosis and Improve Cardiac Remodeling Post Myocardial Ischemia Reperfusion Injury

doi: 10.1002/adhm.202303267

Figure Lengend Snippet: The functional synergy of engineered macrophage and coupled liposomes. A) The CD47‐FITC binding capacity of MAC CCR2+MerTK CR ‐Lipo PEP‐20 and various control groups under the long‐short arm designs was validated by flow cytometry ( n = 3). B) The SIRPα expression level of MAC CCR2+MerTK CR ‐Lipo PEP‐20 and various control groups under the long‐short arm designs was detected by flow cytometry ( n = 3). C) Flow cytometry analysis of the ROS‐responsive release of liposomes anchored on engineered macrophages ( n = 3). D) Flow cytometry analysis of the ROS‐responsive release of PEP‐20 loaded on MAC CCR2+MerTK CR ‐Lipo PEP‐20 ( n = 3). E) Confocal imaging and F) flow cytometry analysis of the optimization of synergically loaded PEP‐20 on engineered macrophage's efferocytosis ( n = 3). Results are presented as mean ± SD, ns p > 0.05, * p < 0.05, * p < 0.01, * p < 0.001.

Article Snippet: FITC‐labeled CD47 protein (CD7‐HF3H3, ACRO Biosystems, China) or isotype control (BSA‐FITC, WH0092607, Weihua Bio, China) was used for the detection of PEP‐20.

Techniques: Functional Assay, Liposomes, Binding Assay, Control, Flow Cytometry, Expressing, Imaging