cd34  (Abcam)

 
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    Name:
    Anti CD34 antibody
    Description:

    Catalog Number:
    AB150060
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    Structured Review

    Abcam cd34
    Identification of bone marrow mesenchymal stem cells (BMSCs) (immunocytochemical staining). (A–D) The BMSCs were characterized by immunocytochemistry for CD44 (A), CD90 (B), CD29 (C) and <t>CD34</t> (D). BMSCs were positive for CD44 (green), CD90 (green), and CD29 staining (red), and nearly negative for CD34 staining. Magnification: 40×. (E) Quantification of markers for BMSCs.

    https://www.bioz.com/result/cd34/product/Abcam
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd34 - by Bioz Stars, 2021-06
    94/100 stars

    Images

    1) Product Images from "12 hours after cerebral ischemia is the optimal time for bone marrow mesenchymal stem cell transplantation"

    Article Title: 12 hours after cerebral ischemia is the optimal time for bone marrow mesenchymal stem cell transplantation

    Journal: Neural Regeneration Research

    doi: 10.4103/1673-5374.158354

    Identification of bone marrow mesenchymal stem cells (BMSCs) (immunocytochemical staining). (A–D) The BMSCs were characterized by immunocytochemistry for CD44 (A), CD90 (B), CD29 (C) and CD34 (D). BMSCs were positive for CD44 (green), CD90 (green), and CD29 staining (red), and nearly negative for CD34 staining. Magnification: 40×. (E) Quantification of markers for BMSCs.
    Figure Legend Snippet: Identification of bone marrow mesenchymal stem cells (BMSCs) (immunocytochemical staining). (A–D) The BMSCs were characterized by immunocytochemistry for CD44 (A), CD90 (B), CD29 (C) and CD34 (D). BMSCs were positive for CD44 (green), CD90 (green), and CD29 staining (red), and nearly negative for CD34 staining. Magnification: 40×. (E) Quantification of markers for BMSCs.

    Techniques Used: Staining, Immunocytochemistry

    Related Articles

    Blocking Assay:

    Article Title: Longitudinal MRI study after carbon ion and photon irradiation: shorter latency time for myelopathy is not associated with differential morphological changes
    Article Snippet: .. After blocking in 10% normal goat serum, sections were incubated with the primary antibodies monoclonal mouse anti-EBA SMI 71 (1:500, Biolegend, San Diego, Ca, US) and recombinant rabbit anti-CD34 antibody (1:1000, abcam, Cambridge, UK) in 3% bovine serum albumin in TBS-T at 4 °C over night. .. For detection, sections were incubated with the fluorescent-coupled secondary antibodies goat anti-rabbit Alexa Flour 555 (1:1000, Molecular Probes®, Invitrogen AG, Carlsbad, USA) and goat anti-mouse Alexa Fluor 488 (1:1000, Molecular Probes®) at room temperature for 30 min each.

    Incubation:

    Article Title: Longitudinal MRI study after carbon ion and photon irradiation: shorter latency time for myelopathy is not associated with differential morphological changes
    Article Snippet: .. After blocking in 10% normal goat serum, sections were incubated with the primary antibodies monoclonal mouse anti-EBA SMI 71 (1:500, Biolegend, San Diego, Ca, US) and recombinant rabbit anti-CD34 antibody (1:1000, abcam, Cambridge, UK) in 3% bovine serum albumin in TBS-T at 4 °C over night. .. For detection, sections were incubated with the fluorescent-coupled secondary antibodies goat anti-rabbit Alexa Flour 555 (1:1000, Molecular Probes®, Invitrogen AG, Carlsbad, USA) and goat anti-mouse Alexa Fluor 488 (1:1000, Molecular Probes®) at room temperature for 30 min each.

    Article Title: Cellular Factor XIII, a Transglutaminase in Human Corneal Keratocytes
    Article Snippet: For double labeling immunoreactions, slides were first incubated with rabbit anti-human FXIII-A antibody, then with FITC conjugated goat anti-rabbit IgG. .. CD34 antigen was detected by incubation with monoclonal anti-human CD34 antibody (Abcam, Cambridge, UK). .. The reaction was visualized by biotinylated horse anti-mouse IgG (Vector Labs) followed by Texas Red-labeled streptavidin (Vector Labs).

    Article Title: Shixiang Plaster, a Traditional Chinese Medicine, Promotes Healing in a Rat Model of Diabetic Ulcer Through the receptor for Advanced Glycation End Products (RAGE)/Nuclear Factor kappa B (NF-κB) and Vascular Endothelial Growth Factor (VEGF)/Vascular Cell Adhesion Molecule-1 (VCAM-1)/Endothelial Nitric Oxide Synthase (eNOS) Signaling Pathways
    Article Snippet: The tissue sections were incubated at 4°C overnight with the primary antibodies. .. The primary antibodies were incubated on the tissue sections overnight at 4°C and included rabbit anti-rat advanced glycosylation end products (AGEs) polyclonal antibody (1: 2000) (Cat. No. ab23722) (Abcam, Cambridge, MA, USA), rabbit anti-rat vascular endothelial growth factor (VEGF) polyclonal antibody (1: 2000) (Cat. No. ab53465) (Abcam, Cambridge, MA, USA), rabbit anti-rat CD34 monoclonal antibody (1: 3000) (Cat. No. ab185732) (Abcam, Cambridge, MA, USA). .. The tissue sections were washed with PBS and incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (1: 1000) (Cat. No. ab6721) (Abcam, Cambridge, MA, USA) at 37°C for 1 h. The tissues were then stained with DAB and hematoxylin for 3 min, differentiated with 0.1% alcohol hydrochloride for 3 min, and dehydrated with graded alcohols for 3 min. Tissue sections were counterstained with hematoxylin, mounted, and coverslipped.

    Recombinant:

    Article Title: Longitudinal MRI study after carbon ion and photon irradiation: shorter latency time for myelopathy is not associated with differential morphological changes
    Article Snippet: .. After blocking in 10% normal goat serum, sections were incubated with the primary antibodies monoclonal mouse anti-EBA SMI 71 (1:500, Biolegend, San Diego, Ca, US) and recombinant rabbit anti-CD34 antibody (1:1000, abcam, Cambridge, UK) in 3% bovine serum albumin in TBS-T at 4 °C over night. .. For detection, sections were incubated with the fluorescent-coupled secondary antibodies goat anti-rabbit Alexa Flour 555 (1:1000, Molecular Probes®, Invitrogen AG, Carlsbad, USA) and goat anti-mouse Alexa Fluor 488 (1:1000, Molecular Probes®) at room temperature for 30 min each.

    Staining:

    Article Title: Tie2 in Tumor Endothelial Signaling and Survival: Implications for Antiangiogenic Therapy
    Article Snippet: Staining for endothelial cell p-AKT, p-ERK, TUNEL, and Ki-67 expression were done as described previously ( , ). .. Paraffin-embedded tumor sections were stained with an anti-p-AKT (Ser473 ) antibody (736E11; Cell Signaling), anti-p-p44/42 mitogen-activated protein kinase (Thr202 /Tyr204 ) antibody (20G11; Cell Signaling), or anti-Ki-67 antibody (Vector Laboratories) and an anti-CD34 antibody (Abcam) to identify endothelial cell p-AKT, p-ERK, or Ki-67, respectively. ..

    Inverted Microscopy:

    Article Title: 12 hours after cerebral ischemia is the optimal time for bone marrow mesenchymal stem cell transplantation
    Article Snippet: .. Under the inverted microscope (Olympus, Tokyo, Japan), MSCs were processed immunocytochemically for CD44 (ab157107, 1:200; Abcam, Cambridge, MA, USA), CD90 (ab225, 1:1,000; Abcam), CD29 (04-1109, 1:250; Millipore, Billerica, MA, USA) and CD34 (ab185732, 1:200; Abcam) (Lindvall and Kokaia, 2006). .. Passage 4 MSCs (2.0 × 105 ) were diluted in 200 μL PBS and then injected into the rats via the tail vein.

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  • cd34  (Abcam)
    94
    Abcam cd34
    Identification of bone marrow mesenchymal stem cells (BMSCs) (immunocytochemical staining). (A–D) The BMSCs were characterized by immunocytochemistry for CD44 (A), CD90 (B), CD29 (C) and <t>CD34</t> (D). BMSCs were positive for CD44 (green), CD90 (green), and CD29 staining (red), and nearly negative for CD34 staining. Magnification: 40×. (E) Quantification of markers for BMSCs.
    Cd34, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd34/product/Abcam
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd34 - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

    98
    Abcam rabbit anti cd34
    Anoikis resistance in human osteosarcoma cells promotes angiogenesis in a nude mouse xenograft model. (A) Representative immunohistochemical staining of p-Src, VEGF-A and <t>CD34</t> inxenograft tumors. Scale bar, 30 µm. (B) p-Src and VEGF-A expression was higher in anoikis-resistant cells, as was MVD. Results are expressed as the mean ± SD. *P
    Rabbit Anti Cd34, supplied by Abcam, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti cd34/product/Abcam
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti cd34 - by Bioz Stars, 2021-06
    98/100 stars
      Buy from Supplier


    Image Search Results


    Identification of bone marrow mesenchymal stem cells (BMSCs) (immunocytochemical staining). (A–D) The BMSCs were characterized by immunocytochemistry for CD44 (A), CD90 (B), CD29 (C) and CD34 (D). BMSCs were positive for CD44 (green), CD90 (green), and CD29 staining (red), and nearly negative for CD34 staining. Magnification: 40×. (E) Quantification of markers for BMSCs.

    Journal: Neural Regeneration Research

    Article Title: 12 hours after cerebral ischemia is the optimal time for bone marrow mesenchymal stem cell transplantation

    doi: 10.4103/1673-5374.158354

    Figure Lengend Snippet: Identification of bone marrow mesenchymal stem cells (BMSCs) (immunocytochemical staining). (A–D) The BMSCs were characterized by immunocytochemistry for CD44 (A), CD90 (B), CD29 (C) and CD34 (D). BMSCs were positive for CD44 (green), CD90 (green), and CD29 staining (red), and nearly negative for CD34 staining. Magnification: 40×. (E) Quantification of markers for BMSCs.

    Article Snippet: Under the inverted microscope (Olympus, Tokyo, Japan), MSCs were processed immunocytochemically for CD44 (ab157107, 1:200; Abcam, Cambridge, MA, USA), CD90 (ab225, 1:1,000; Abcam), CD29 (04-1109, 1:250; Millipore, Billerica, MA, USA) and CD34 (ab185732, 1:200; Abcam) (Lindvall and Kokaia, 2006).

    Techniques: Staining, Immunocytochemistry

    Anoikis resistance in human osteosarcoma cells promotes angiogenesis in a nude mouse xenograft model. (A) Representative immunohistochemical staining of p-Src, VEGF-A and CD34 inxenograft tumors. Scale bar, 30 µm. (B) p-Src and VEGF-A expression was higher in anoikis-resistant cells, as was MVD. Results are expressed as the mean ± SD. *P

    Journal: Oncology Reports

    Article Title: Anoikis-resistant human osteosarcoma cells display significant angiogenesis by activating the Src kinase-mediated MAPK pathway

    doi: 10.3892/or.2018.6827

    Figure Lengend Snippet: Anoikis resistance in human osteosarcoma cells promotes angiogenesis in a nude mouse xenograft model. (A) Representative immunohistochemical staining of p-Src, VEGF-A and CD34 inxenograft tumors. Scale bar, 30 µm. (B) p-Src and VEGF-A expression was higher in anoikis-resistant cells, as was MVD. Results are expressed as the mean ± SD. *P

    Article Snippet: For mouse xenograft specimens, primary rabbit anti-p-Src (dilution 1:200; cat. no. 2105; Cell Signaling Technology, Inc.), mouse anti-VEGF (dilution 1:50; cat. no. sc-7269; Santa Cruz Biotechnology, Inc.) and rabbit anti-CD34 (dilution 1:100; cat. no. Ab81289; Abcam, Cambridge, MA, USA) were used.

    Techniques: Immunohistochemistry, Staining, Expressing