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Journal: Advanced Science
Article Title: Tumor‐Intrinsic HEXB Drives Endothelial‐To‐Mesenchymal Transition and Confers Resistance to Anti‐Angiogenic Therapy in Glioblastoma
doi: 10.1002/advs.78208
Figure Lengend Snippet: Glioma‐intrinsic HEXB promoted Endo‐MT changes of ECs. (A) Survival of GBM patients received bevacizumab treatment with different HEXB expression in GSE79671 (log‐rank test). (B) Survival of GBM patients received bevacizumab treatment with different HEXB expression in GSE72951 (log‐rank test). (C) The rate of response patients was lower in GBM patients with high HEXB expression in GSE72951 (Patients with a survival time superior to the median survival of the standard treatment were defined as the responder group, whereas those with a shorter survival time were defined as the non‐responder group). (D) The analysis of migration ability of HBMECs treated with different CM (sh#1: shHEXB#1; sh#2: shHEXB#2). (E) Permeability changes of HBMECs treated with different CM (sh#1: shHEXB#1; sh#2: shHEXB#2). (F) Immunofluorescence staining image of HBMECs treated with different CM (sh#1: shHEXB#1; sh#2: shHEXB#2). (G) Western blot results of HBMECs treated with different CM (sh#1: shHEXB#1; sh#2: shHEXB#2). (H) PCR results of HBMECs treated with different CM (sh#1: shHEXB#1; sh#2: shHEXB#2). (I) Flow cytometry of CD31 + cells in tumors from GSC1‐bearing mice. (J) Flow cytometry of CD31 + cells in tumors from GSC21‐bearing mice. (Date were presented as means ± standard deviations, * indicates p ≤ 0.05, ** indicates p < 0.01, **** indicates p < 0.0001.).
Article Snippet: The
Techniques: Expressing, Analysis, Migration, Permeability, Immunofluorescence, Staining, Western Blot, Flow Cytometry