Journal: Molecular Therapy Oncology

Article Title: Engineered Salmonella -mediated c-di-AMP delivery activates STING to remodel the tumor microenvironment

doi: 10.1016/j.omton.2026.201185

Figure Lengend Snippet: Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis of F4/80 and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.

Article Snippet: The following antibodies were used: FITC anti-mouse F4/80 (clone CI: A3-1), APC anti-mouse CD86 (clone GL-1), FITC anti-mouse CD3 (clone 17A2), APC anti-mouse CD4 (clone GK1.5), and APC anti-mouse CD8 (clone YTS-169), all purchased from Elabscience Biotechnology Co., Ltd.

Techniques: Flow Cytometry

Journal: Cell Reports Methods

Article Title: Multiscale domain identification for spatial transcriptomics via persistent homology

doi: 10.1016/j.crmeth.2026.101376

Figure Lengend Snippet: PHD-MS analyzes tumor microenvironment morphology (A) Expert annotated tumors from immunofluorescent image, with anti-CD3 intensity in pink and DAPI intensity in green. IDC is outlined in yellow, carcinoma in situ is outlined in blue, benign hyperplasia is outlined in red, and unclassified tumor is in green. (B) Prominent multiscale domains identified by PHD-MS, selected from the most persistent domains. Regions are numbered to correspond with the labels in (A). (C) PHD-MS heterogeneity map highlights highly heterogeneous regions. The heterogeneity score approximates the number of persistent domains that contain each spot. (D) Mean normalized immune expression from a set of immune genes (PTPRC, CD4, CD8A, CD14, CD68, and IGHG3). (E) Normalized expression of key oncogenes APOE, PGK1, and MALAT1 in the lower IDC. From DGE analysis, APOE is overexpressed in region 7 relative to 8 and 9, PGK1 is overexpressed in 8, and MALAT1 is overexpressed in 9.

Article Snippet: Visium Invasive Ductal Carcinoma Stained With Fluorescent CD3 Antibody , Zhao et al. , https://www.10xgenomics.com/datasets/invasive-ductal-carcinoma-stained-with-fluorescent-cd-3-antibody-1-standard-1-2-0.

Techniques: In Situ, Expressing

Journal: bioRxiv

Article Title: Early-life mucosal T cells direct intestinal stem cell fate via a coordinated developmental program

doi: 10.64898/2026.05.08.723752

Figure Lengend Snippet: (A) Co-culture strategy (created with Biorender.com ). Small intestine (SI) was cryopreserved, and stable organoid lines were generated. T cells were isolated from SI tissue (B-E, H-K) or cord blood (F-G) by positive selection. CD3+ cells were co-cultured with established SI organoid lines generated from fetal (B-I) , neonatal (D-E) , or adult (I-K) donors. (B, D, F, H) Representative images from day 6 of co-culture. Scale bar is 530 µm. (C) Data were generated from one organoid and T cell donor per fetal age group and analyzed by one-way ANOVA with Tukey’s post-hoc test. Only significant comparisons are denoted. Data are shown as means ± SEM. (E, G, I) Data were generated from 2-4 organoid and T cell donors each. Data were analyzed via Mann-Whitney and are shown as medians ± IQR. (K) Data were generated from 3 organoid donors and 4 T cell donors and are normalized to the number of organoids generated in mock (no T cell) conditions, represented as fold change. Each dot represents the average of three technical replicate wells (C) or one well of organoids (E, G, I, K) . Data were analyzed via Welch’s t-test and are shown as means ± SEM. Each experiment was repeated 2-3 independent times. Each dot represents one well of organoids. Significance is indicated as follows: * p<0.05; ** p<0.01; *** p<0.001; **** p<0.0001; ns, not significant (p>0.05).

Article Snippet: T cells were isolated via CD3 microbead positive selection (Miltenyi, cat#130-050-101 or 130-097-043) via MS or LS columns (Miltenyi, cat#130-042-201 or 130-042-401) per manufacturer’s instructions (Miltenyi).

Techniques: Co-Culture Assay, Generated, Isolation, Selection, Cell Culture, MANN-WHITNEY