Journal: Molecular Therapy Oncology

Article Title: Innovative photodynamic therapy using rose bengal for the treatment of human melanoma

doi: 10.1016/j.omton.2026.201241

Figure Lengend Snippet: Evaluation of immunologic response with PDT-RB (A–D) Proliferation of activated PBMCs (anti-CD3 – anti-CD28 cocktail) in co-culture with conditioned media derived from HBL (A and C) and LND (B and D) cell lines, respectively, after 72 h and 120 h of culture. Results are presented as mean ± SEM of 4 independent experiments, one-way ANOVA statistical test was performed with ∗ p < 0.05, ∗∗ p < 0.001 being considered statistically significant. NT, non-treated cells; Illu, illuminated alone without Rose Bengal; RB, Rose Bengal; PDT-RB, cells incubated with RB and illuminated.

Article Snippet: PBMCs were either stimulated or not stimulated with anti-CD3 (0.25 μg/mL; Miltenyi, Bergisch Glad bach, Germany) and anti-CD28 (0.25 μg/mL; Clinisciences, Montrouge, France).

Techniques: Co-Culture Assay, Derivative Assay, Incubation

Journal: Cancer research

Article Title: Targeting the αvβ5 Integrin Modifies the TGF-β-Rich Tumor Microenvironment of Pancreatic Cancer

doi: 10.1158/0008-5472.CAN-25-4223

Figure Lengend Snippet: A, Representative confocal images of Tregs in human PDAC and spleen specimens collected from an identical patient. The dotted lines outline cancer ducts. Red, CD3; white, Foxp3; green, αvβ5 integrin; blue, DAPI. Arrowheads, αvβ5 integrin + Tregs. The boxed area is magnified. Scale bars, 20 μm (large panels); 10 μm (small panels). The bar diagram summarizes the average number of αvβ5 + Tregs in the PDAC and spleen from 5 patients. The cells were counted in 5 randomly chosen HPFs per section. B, Flow cytometry analysis of naïve human CD4 + T cells before (left) and after (right) a 3-day-treatment with anti-CD3/CD28 Abs and TGF-β1. Note the induction of CD25 + Foxp3 + iTregs (red) that express αvβ5 integrin. CD25 − Foxp3 − non-Tregs (blue) remain negative for the integrin. Three biological replicates per group were analyzed. C, Representative microscopic images of αvβ5 integrin (blue) expressed in one of the five human PDAC specimens analyzed. Red box, with cancer ducts; green box, without cancer ducts. Black arrowhead, cancer duct; white arrowhead, stroma cell. Scale bars, 500 μm (left); 50 μm (middle); 20 μm (right). Statistical analysis, Student’s t test. Error bars, mean ± standard error.

Article Snippet: The CD4 + T cells were cultured for 3 days with or without KPC78 cells in the presence of low dose anti-CD3/CD28 beads (x40 dilution; Cat# 130-095-925; Miltenyi Biotec), recombinant mouse TGF-β1 (5 ng/ml; Cat# 7666-MB; R&D Systems, Minneapolis, MN), and recombinant mouse interleukin-2 (mIL-2, 100 U/ml; Cat# 11271164001; Roche, Basel, Switzerland), and subjected to flow cytometry as described elsewhere.

Techniques: Flow Cytometry