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Polyplus Transfection cd21 cdna orf clone human n myc tag
Cd21 Cdna Orf Clone Human N Myc Tag, supplied by Polyplus Transfection, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd21 cdna orf clone human n myc tag/product/Polyplus Transfection
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
cd21 cdna orf clone human n myc tag - by Bioz Stars, 2021-06
86/100 stars

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Flow Cytometry:

Article Title: The Sez6 family inhibits complement at the level of the C3 convertase
Article Snippet: Background values were obtained from wells not coated with C4b but incubated with C2 and C1s-enzyme. .. Flow Cytometry CHO (Freestyle) cells were grown in serum-free Freestyle media (Gibco, 12651-014) and transfected with human cDNA expression plasmids with N-terminal Myc tags obtained from Sinobiological: M-SEZ6L2 (HG13969-NM; modified from NCBI RefSeq BC000567; similar to NM 001114100.2; Uniprot ACC: Q6UXD5-6;), M-SEZ6 (HG13436-NM; modified from NCBI RefSeq NM 178860.4; Uniprot ACC: Q53EL9-1;), M-SEZ6L (HG20982-NM; modified from NM 001184773.1 and contains the point mutation 1428A/C that does not alter the amino acid sequence; Uniprot ACC: Q9BYH1-6), M-MCP (HG12239-NM; modified from NCBI RefSeq BC030594 ), or M-CR2 (HG10811-NM; modified from NCBI RefSeq NM 001877.3 ) using FectoPro transfection reagent (Polyplus, 116-001). .. Alternatively cells were transfected with a GFP expression plasmid or co-transfected with GFP and M-SEZ6L2 or HIS-tagged DAF (H-DAF; Sinobiological HG10101-NH; modified from NCBI RefSeq NM 000574.3 ).

Article Title: The Sez6 Family Inhibits Complement by Facilitating Factor I Cleavage of C3b and Accelerating the Decay of C3 Convertases
Article Snippet: Background values were obtained from wells not coated with C4b but incubated with C2 and C1s-enzyme. .. Flow Cytometry CHO cells (a Chinese hamster ovary cell line) were grown in serum-free Freestyle media (Gibco, 12651-014) and transfected with human cDNA expression plasmids with N-terminal Myc-tags obtained from Sino Biological: M-SEZ6L2 (HG13969-NM; modified from NCBI RefSeq BC000567, similar to NM_001114100.2; Uniprot ACC: Q6UXD5-6), M-SEZ6 (HG13436-NM; modified from NCBI RefSeq NM_178860.4; Uniprot ACC: Q53EL9-1), M-SEZ6L (HG20982-NM; modified from NM_001184773.1; Uniprot ACC: Q9BYH1-6), M-MCP (HG12239-NM; modified from NCBI RefSeq BC030594), or M-CR2 (HG10811-NM; modified from NCBI RefSeq NM_001877.3) using FectoPro transfection reagent (Polyplus, 116-001). .. Alternatively, cells were transfected with a GFP expression plasmid or co-transfected with GFP and M-SEZ6L2 or HIS-tagged DAF (H-DAF; Sino Biological HG10101-NH; modified from NCBI RefSeq NM_000574.3).

Transfection:

Article Title: The Sez6 family inhibits complement at the level of the C3 convertase
Article Snippet: Background values were obtained from wells not coated with C4b but incubated with C2 and C1s-enzyme. .. Flow Cytometry CHO (Freestyle) cells were grown in serum-free Freestyle media (Gibco, 12651-014) and transfected with human cDNA expression plasmids with N-terminal Myc tags obtained from Sinobiological: M-SEZ6L2 (HG13969-NM; modified from NCBI RefSeq BC000567; similar to NM 001114100.2; Uniprot ACC: Q6UXD5-6;), M-SEZ6 (HG13436-NM; modified from NCBI RefSeq NM 178860.4; Uniprot ACC: Q53EL9-1;), M-SEZ6L (HG20982-NM; modified from NM 001184773.1 and contains the point mutation 1428A/C that does not alter the amino acid sequence; Uniprot ACC: Q9BYH1-6), M-MCP (HG12239-NM; modified from NCBI RefSeq BC030594 ), or M-CR2 (HG10811-NM; modified from NCBI RefSeq NM 001877.3 ) using FectoPro transfection reagent (Polyplus, 116-001). .. Alternatively cells were transfected with a GFP expression plasmid or co-transfected with GFP and M-SEZ6L2 or HIS-tagged DAF (H-DAF; Sinobiological HG10101-NH; modified from NCBI RefSeq NM 000574.3 ).

Article Title: The Sez6 Family Inhibits Complement by Facilitating Factor I Cleavage of C3b and Accelerating the Decay of C3 Convertases
Article Snippet: Background values were obtained from wells not coated with C4b but incubated with C2 and C1s-enzyme. .. Flow Cytometry CHO cells (a Chinese hamster ovary cell line) were grown in serum-free Freestyle media (Gibco, 12651-014) and transfected with human cDNA expression plasmids with N-terminal Myc-tags obtained from Sino Biological: M-SEZ6L2 (HG13969-NM; modified from NCBI RefSeq BC000567, similar to NM_001114100.2; Uniprot ACC: Q6UXD5-6), M-SEZ6 (HG13436-NM; modified from NCBI RefSeq NM_178860.4; Uniprot ACC: Q53EL9-1), M-SEZ6L (HG20982-NM; modified from NM_001184773.1; Uniprot ACC: Q9BYH1-6), M-MCP (HG12239-NM; modified from NCBI RefSeq BC030594), or M-CR2 (HG10811-NM; modified from NCBI RefSeq NM_001877.3) using FectoPro transfection reagent (Polyplus, 116-001). .. Alternatively, cells were transfected with a GFP expression plasmid or co-transfected with GFP and M-SEZ6L2 or HIS-tagged DAF (H-DAF; Sino Biological HG10101-NH; modified from NCBI RefSeq NM_000574.3).

Expressing:

Article Title: The Sez6 family inhibits complement at the level of the C3 convertase
Article Snippet: Background values were obtained from wells not coated with C4b but incubated with C2 and C1s-enzyme. .. Flow Cytometry CHO (Freestyle) cells were grown in serum-free Freestyle media (Gibco, 12651-014) and transfected with human cDNA expression plasmids with N-terminal Myc tags obtained from Sinobiological: M-SEZ6L2 (HG13969-NM; modified from NCBI RefSeq BC000567; similar to NM 001114100.2; Uniprot ACC: Q6UXD5-6;), M-SEZ6 (HG13436-NM; modified from NCBI RefSeq NM 178860.4; Uniprot ACC: Q53EL9-1;), M-SEZ6L (HG20982-NM; modified from NM 001184773.1 and contains the point mutation 1428A/C that does not alter the amino acid sequence; Uniprot ACC: Q9BYH1-6), M-MCP (HG12239-NM; modified from NCBI RefSeq BC030594 ), or M-CR2 (HG10811-NM; modified from NCBI RefSeq NM 001877.3 ) using FectoPro transfection reagent (Polyplus, 116-001). .. Alternatively cells were transfected with a GFP expression plasmid or co-transfected with GFP and M-SEZ6L2 or HIS-tagged DAF (H-DAF; Sinobiological HG10101-NH; modified from NCBI RefSeq NM 000574.3 ).

Article Title: The Sez6 Family Inhibits Complement by Facilitating Factor I Cleavage of C3b and Accelerating the Decay of C3 Convertases
Article Snippet: Background values were obtained from wells not coated with C4b but incubated with C2 and C1s-enzyme. .. Flow Cytometry CHO cells (a Chinese hamster ovary cell line) were grown in serum-free Freestyle media (Gibco, 12651-014) and transfected with human cDNA expression plasmids with N-terminal Myc-tags obtained from Sino Biological: M-SEZ6L2 (HG13969-NM; modified from NCBI RefSeq BC000567, similar to NM_001114100.2; Uniprot ACC: Q6UXD5-6), M-SEZ6 (HG13436-NM; modified from NCBI RefSeq NM_178860.4; Uniprot ACC: Q53EL9-1), M-SEZ6L (HG20982-NM; modified from NM_001184773.1; Uniprot ACC: Q9BYH1-6), M-MCP (HG12239-NM; modified from NCBI RefSeq BC030594), or M-CR2 (HG10811-NM; modified from NCBI RefSeq NM_001877.3) using FectoPro transfection reagent (Polyplus, 116-001). .. Alternatively, cells were transfected with a GFP expression plasmid or co-transfected with GFP and M-SEZ6L2 or HIS-tagged DAF (H-DAF; Sino Biological HG10101-NH; modified from NCBI RefSeq NM_000574.3).

Modification:

Article Title: The Sez6 family inhibits complement at the level of the C3 convertase
Article Snippet: Background values were obtained from wells not coated with C4b but incubated with C2 and C1s-enzyme. .. Flow Cytometry CHO (Freestyle) cells were grown in serum-free Freestyle media (Gibco, 12651-014) and transfected with human cDNA expression plasmids with N-terminal Myc tags obtained from Sinobiological: M-SEZ6L2 (HG13969-NM; modified from NCBI RefSeq BC000567; similar to NM 001114100.2; Uniprot ACC: Q6UXD5-6;), M-SEZ6 (HG13436-NM; modified from NCBI RefSeq NM 178860.4; Uniprot ACC: Q53EL9-1;), M-SEZ6L (HG20982-NM; modified from NM 001184773.1 and contains the point mutation 1428A/C that does not alter the amino acid sequence; Uniprot ACC: Q9BYH1-6), M-MCP (HG12239-NM; modified from NCBI RefSeq BC030594 ), or M-CR2 (HG10811-NM; modified from NCBI RefSeq NM 001877.3 ) using FectoPro transfection reagent (Polyplus, 116-001). .. Alternatively cells were transfected with a GFP expression plasmid or co-transfected with GFP and M-SEZ6L2 or HIS-tagged DAF (H-DAF; Sinobiological HG10101-NH; modified from NCBI RefSeq NM 000574.3 ).

Article Title: The Sez6 Family Inhibits Complement by Facilitating Factor I Cleavage of C3b and Accelerating the Decay of C3 Convertases
Article Snippet: Background values were obtained from wells not coated with C4b but incubated with C2 and C1s-enzyme. .. Flow Cytometry CHO cells (a Chinese hamster ovary cell line) were grown in serum-free Freestyle media (Gibco, 12651-014) and transfected with human cDNA expression plasmids with N-terminal Myc-tags obtained from Sino Biological: M-SEZ6L2 (HG13969-NM; modified from NCBI RefSeq BC000567, similar to NM_001114100.2; Uniprot ACC: Q6UXD5-6), M-SEZ6 (HG13436-NM; modified from NCBI RefSeq NM_178860.4; Uniprot ACC: Q53EL9-1), M-SEZ6L (HG20982-NM; modified from NM_001184773.1; Uniprot ACC: Q9BYH1-6), M-MCP (HG12239-NM; modified from NCBI RefSeq BC030594), or M-CR2 (HG10811-NM; modified from NCBI RefSeq NM_001877.3) using FectoPro transfection reagent (Polyplus, 116-001). .. Alternatively, cells were transfected with a GFP expression plasmid or co-transfected with GFP and M-SEZ6L2 or HIS-tagged DAF (H-DAF; Sino Biological HG10101-NH; modified from NCBI RefSeq NM_000574.3).

Mutagenesis:

Article Title: The Sez6 family inhibits complement at the level of the C3 convertase
Article Snippet: Background values were obtained from wells not coated with C4b but incubated with C2 and C1s-enzyme. .. Flow Cytometry CHO (Freestyle) cells were grown in serum-free Freestyle media (Gibco, 12651-014) and transfected with human cDNA expression plasmids with N-terminal Myc tags obtained from Sinobiological: M-SEZ6L2 (HG13969-NM; modified from NCBI RefSeq BC000567; similar to NM 001114100.2; Uniprot ACC: Q6UXD5-6;), M-SEZ6 (HG13436-NM; modified from NCBI RefSeq NM 178860.4; Uniprot ACC: Q53EL9-1;), M-SEZ6L (HG20982-NM; modified from NM 001184773.1 and contains the point mutation 1428A/C that does not alter the amino acid sequence; Uniprot ACC: Q9BYH1-6), M-MCP (HG12239-NM; modified from NCBI RefSeq BC030594 ), or M-CR2 (HG10811-NM; modified from NCBI RefSeq NM 001877.3 ) using FectoPro transfection reagent (Polyplus, 116-001). .. Alternatively cells were transfected with a GFP expression plasmid or co-transfected with GFP and M-SEZ6L2 or HIS-tagged DAF (H-DAF; Sinobiological HG10101-NH; modified from NCBI RefSeq NM 000574.3 ).

Sequencing:

Article Title: The Sez6 family inhibits complement at the level of the C3 convertase
Article Snippet: Background values were obtained from wells not coated with C4b but incubated with C2 and C1s-enzyme. .. Flow Cytometry CHO (Freestyle) cells were grown in serum-free Freestyle media (Gibco, 12651-014) and transfected with human cDNA expression plasmids with N-terminal Myc tags obtained from Sinobiological: M-SEZ6L2 (HG13969-NM; modified from NCBI RefSeq BC000567; similar to NM 001114100.2; Uniprot ACC: Q6UXD5-6;), M-SEZ6 (HG13436-NM; modified from NCBI RefSeq NM 178860.4; Uniprot ACC: Q53EL9-1;), M-SEZ6L (HG20982-NM; modified from NM 001184773.1 and contains the point mutation 1428A/C that does not alter the amino acid sequence; Uniprot ACC: Q9BYH1-6), M-MCP (HG12239-NM; modified from NCBI RefSeq BC030594 ), or M-CR2 (HG10811-NM; modified from NCBI RefSeq NM 001877.3 ) using FectoPro transfection reagent (Polyplus, 116-001). .. Alternatively cells were transfected with a GFP expression plasmid or co-transfected with GFP and M-SEZ6L2 or HIS-tagged DAF (H-DAF; Sinobiological HG10101-NH; modified from NCBI RefSeq NM 000574.3 ).

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    Polyplus Transfection cd21 cdna orf clone human n myc tag
    Cd21 Cdna Orf Clone Human N Myc Tag, supplied by Polyplus Transfection, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd21 cdna orf clone human n myc tag/product/Polyplus Transfection
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd21 cdna orf clone human n myc tag - by Bioz Stars, 2021-06
    86/100 stars
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