Journal: Glycobiology
Article Title: Quantitative analysis of chondroitin sulfate disaccharides from human and rodent fixed brain tissue by electrospray ionization-tandem mass spectrometry
doi: 10.1093/glycob/cwz060
Figure Lengend Snippet: LC-MS/MS + MRM profile of CS disaccharides isolated from fixed human brain tissue. LC separation and MS/MS-MRM analysis were used to determine the composition of chondroitin sulfates extracted from the primary human motor cortex. Extracted disaccharides are identified using the ion chromatograms with indicated channels. The percentage of each CS isomers is shown at the bottom with the mean of triplicates from a single human donor followed by the standard error in parenthesis. The Δ2S6S-CS isolated from fixed tissue was within the LOD (see Figure S7) but not within the LOQ, and thus denoted as N.Q., not quantifiable.
Article Snippet: The Δ2S6S-CS (CD006) disaccharide standard was purchased from Galen Laboratory Supplies (North Haven, CT).
Techniques: Liquid Chromatography with Mass Spectroscopy, Isolation, Tandem Mass Spectroscopy