cba isothermal assembly mastermix  (New England Biolabs)


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    New England Biolabs cba isothermal assembly mastermix
    Cba Isothermal Assembly Mastermix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cba isothermal assembly mastermix/product/New England Biolabs
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cba isothermal assembly mastermix - by Bioz Stars, 2020-07
    88/100 stars

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    Crocin Bleaching Assay:

    Article Title: Farnesylated and methylated KRAS4b: high yield production of protein suitable for biophysical studies of prenylated protein-lipid interactions
    Article Snippet: .. To make the linear cassette, 25 fmoles of each fragment were combined in a 20 ul isothermal assembly reaction with 2× CBA isothermal assembly mastermix (320 μl of 5 × ISO Reaction Buffer [25% polyethylene glycol 8000, 500 mM tris-HCl (pH 8.0), 50 mM MgCl2 , 50 mM dithiothreitol (DTT), 1.0 μM each dNTPs, 5 μM nicotinamide adenine dinucleotide, 6.4 μl of T5 Exonuclease (1 U/ml) (Epicentre), 20 μl of Phusion polymerase (2 U/ml) [New England Biolabs (NEB)], 80 μl of Taq ligase (40 U/ml) (NEB), and 374 μl of deionized H2 O . .. The cassette was amplified using 1ul of the isothermal assembly reaction, with primers 15165 and 15169 (0.4 μM) in a PCR reaction with 2× Phusion HF mastermix, (100 ul, 25 cycle, 60 °C melting temperature, 6 minute extension) and purified using the Qiagen Qiaquick PCR purification kit.

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    New England Biolabs cba isothermal assembly mastermix
    Cba Isothermal Assembly Mastermix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cba isothermal assembly mastermix/product/New England Biolabs
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cba isothermal assembly mastermix - by Bioz Stars, 2020-07
    88/100 stars
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