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Millipore caveoli
Caveoli, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/caveoli/product/Millipore
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
caveoli - by Bioz Stars, 2020-04
85/100 stars

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clathrin
inhibitors against dynamin dynasore

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Recombinant:

Article Title: Immunomodulatory Glycan Lacto-N-Fucopentaose III Requires Clathrin-Mediated Endocytosis To Induce Alternative Activation of Antigen-Presenting Cells
Article Snippet: Inhibitors against dynamin (dynasore), clathrin (chlorpromazine and monodansylcadaverine), and caveoli (filipin and methyl-β-cyclodextrin) were purchased from Sigma-Aldrich, St. Louis, MO, USA. .. Recombinant IL-4 was purchased from R & D systems (Minneapolis, MN, USA).

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  • 99
    Millipore caveolin 1 scaffolding domain peptide
    Effects of genistein, okadaic acid, and <t>caveolin-1</t> peptide on TFV infection. (A) Genistein effect on TFV infection as measured by immunofluorescence staining analysis. Cells were pretreated for 2 h with various concentrations of genistein, as indicated,
    Caveolin 1 Scaffolding Domain Peptide, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caveolin 1 scaffolding domain peptide/product/Millipore
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    caveolin 1 scaffolding domain peptide - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    91
    Millipore caveolin mediated endocytosis
    Clathrin and <t>caveolin-1</t> are expressed in cultured podocytes. A : Western blot analysis shows both clathrin and caveolin-1 are expressed in cultured podocytes. B : in podocytes fixed after 10 min of incubation in FITC-albumin (green), immunostaining of clathrin
    Caveolin Mediated Endocytosis, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caveolin mediated endocytosis/product/Millipore
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    caveolin mediated endocytosis - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    90
    Millipore phospho caveolin 1
    Lipid raft preparations demonstrating increased the presence of p-PKC α , phosphorylated and total <t>caveolin-1</t> (p-Cav-1 and t-Cav-1, respectively) and phosphorylated cSrc (p-cSrc) in the same raft fractions 1 h after viral infection (V) as compared to mock infection (M), and the effect of chemical inhibition of cSrc by PP2 (A) and (B), and of PKC α by calphostin C (Cal.C) in (C). (A) Western blot for p-PKC α shows increased signal upon viral infection, with slight reduction upon PP2 treatment (10 µ M, or DMSO control). (B) Western blot for p-Cav-1, with or without pretreatment by PP2 (or DMSO control), stripped and reprobed for p-cSrc and then stripped and reprobed for t-Cav-1, shows increased p-Cav-1 and p-cSrc in the same lipid raft fractions with a reduction in phosphorylation for both in cells pretreated with PP2. t-Cav-1 in the lipid raft fractions was also slightly reduced by PP2 pretreatment. (C) Western blots on cells pretreated with either DMSO control or calphostin C (1 µ M) for 3 h prior to mock or virus infection, and stripped and reprobed as in (B), show a dramatic reduction in p-Cav-1 but not t-Cav-1, and complete abrogation of p-cSrc expression by calphostin C in the same lipid raft fractions.
    Phospho Caveolin 1, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho caveolin 1/product/Millipore
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho caveolin 1 - by Bioz Stars, 2020-04
    90/100 stars
      Buy from Supplier

    Image Search Results


    Effects of genistein, okadaic acid, and caveolin-1 peptide on TFV infection. (A) Genistein effect on TFV infection as measured by immunofluorescence staining analysis. Cells were pretreated for 2 h with various concentrations of genistein, as indicated,

    Journal: Journal of Virology

    Article Title: Entry of Tiger Frog Virus (an Iridovirus) into HepG2 Cells via a pH-Dependent, Atypical, Caveola-Mediated Endocytosis Pathway ▿

    doi: 10.1128/JVI.01500-10

    Figure Lengend Snippet: Effects of genistein, okadaic acid, and caveolin-1 peptide on TFV infection. (A) Genistein effect on TFV infection as measured by immunofluorescence staining analysis. Cells were pretreated for 2 h with various concentrations of genistein, as indicated,

    Article Snippet: Caveolin-1 scaffolding domain peptide (caveolin-1 peptide), corresponding to full-length caveolin-1 (amino acids 82 to 101) and synthesized as a fusion peptide to the C terminus of the Antennapedia internalization sequence, was purchased from Calbiochem (San Diego, CA).

    Techniques: Infection, Immunofluorescence, Staining

    Immunofluorescence staining analysis. (A) Effects of caveolin-1 peptide on transferrin and CTxB uptake. Cells were untreated (as a positive control) or pretreated with 5 μM caveolin-1 peptide for 2 h at 27°C and washed with PBS. The cells

    Journal: Journal of Virology

    Article Title: Entry of Tiger Frog Virus (an Iridovirus) into HepG2 Cells via a pH-Dependent, Atypical, Caveola-Mediated Endocytosis Pathway ▿

    doi: 10.1128/JVI.01500-10

    Figure Lengend Snippet: Immunofluorescence staining analysis. (A) Effects of caveolin-1 peptide on transferrin and CTxB uptake. Cells were untreated (as a positive control) or pretreated with 5 μM caveolin-1 peptide for 2 h at 27°C and washed with PBS. The cells

    Article Snippet: Caveolin-1 scaffolding domain peptide (caveolin-1 peptide), corresponding to full-length caveolin-1 (amino acids 82 to 101) and synthesized as a fusion peptide to the C terminus of the Antennapedia internalization sequence, was purchased from Calbiochem (San Diego, CA).

    Techniques: Immunofluorescence, Staining, Positive Control

    The caveolin-1 scaffolding domain peptide has a minimal effect on calcium-induced PLD activation. Keratinocytes were preincubated for 24 hours with SFKM containing [ 3 H]oleate and vehicle (0.1% DMS0), 3 μM caveolin-1 scaffolding domain peptide (CSDP) or the negative control (Neg) in medium containing 25 µM calcium (Con) or 125 µM calcium (Ca 2+ ) as indicated. [ 3 H]Phosphatidylethanol levels were then measured as described in Materials and Methods. Values are expressed as the percent control and represent the means ± SEM of 5 separate experiments performed in duplicate; **p

    Journal: PLoS ONE

    Article Title: The Caveolin-1 Scaffolding Domain Peptide Decreases Phosphatidylglycerol Levels and Inhibits Calcium-Induced Differentiation in Mouse Keratinocytes

    doi: 10.1371/journal.pone.0080946

    Figure Lengend Snippet: The caveolin-1 scaffolding domain peptide has a minimal effect on calcium-induced PLD activation. Keratinocytes were preincubated for 24 hours with SFKM containing [ 3 H]oleate and vehicle (0.1% DMS0), 3 μM caveolin-1 scaffolding domain peptide (CSDP) or the negative control (Neg) in medium containing 25 µM calcium (Con) or 125 µM calcium (Ca 2+ ) as indicated. [ 3 H]Phosphatidylethanol levels were then measured as described in Materials and Methods. Values are expressed as the percent control and represent the means ± SEM of 5 separate experiments performed in duplicate; **p

    Article Snippet: Measurement of DNA Synthesis Near-confluent cultures were refed with SFKM containing 0.1% DMSO as the control (Con), 3 µM cell-permeable caveolin-1 scaffolding domain peptide (Calbiochem, Darmstadt, Germany), 3 µM cell-permeable caveolin-1 scaffolding domain peptide negative control (Neg, a scrambled version of the caveolin-1 scaffolding domain peptide), 125 µM CaCl2 [+ 0.1% DMSO (Ca)], 125 µM CaCl2 + 3 µM cell-permeable caveolin-1 scaffolding domain peptide (Ca + CSDP), and 125 µM CaCl2 + 3 µM cell-permeable caveolin-1 scaffolding domain peptide negative control (Ca + Neg).

    Techniques: Scaffolding, Activation Assay, Negative Control

    The caveolin-1 scaffolding domain peptide decreases calcium-increased phosphatidylglycerol levels. Keratinocytes were treated for 24 hours with SFKM containing vehicle (0.1% DMSO) or 3 μM caveolin-1 scaffolding domain peptide (CSDP) or the negative control (Neg) in medium containing 25 μM calcium (Con) or 125 μM calcium (Ca 2+ ) as indicated.. [ 14 C]Phosphatidylglycerol levels were then measured as described in Materials and Methods. Values are expressed as the percent control and represent the means ± SEM of 3 separate experiments performed in duplicate; **p

    Journal: PLoS ONE

    Article Title: The Caveolin-1 Scaffolding Domain Peptide Decreases Phosphatidylglycerol Levels and Inhibits Calcium-Induced Differentiation in Mouse Keratinocytes

    doi: 10.1371/journal.pone.0080946

    Figure Lengend Snippet: The caveolin-1 scaffolding domain peptide decreases calcium-increased phosphatidylglycerol levels. Keratinocytes were treated for 24 hours with SFKM containing vehicle (0.1% DMSO) or 3 μM caveolin-1 scaffolding domain peptide (CSDP) or the negative control (Neg) in medium containing 25 μM calcium (Con) or 125 μM calcium (Ca 2+ ) as indicated.. [ 14 C]Phosphatidylglycerol levels were then measured as described in Materials and Methods. Values are expressed as the percent control and represent the means ± SEM of 3 separate experiments performed in duplicate; **p

    Article Snippet: Measurement of DNA Synthesis Near-confluent cultures were refed with SFKM containing 0.1% DMSO as the control (Con), 3 µM cell-permeable caveolin-1 scaffolding domain peptide (Calbiochem, Darmstadt, Germany), 3 µM cell-permeable caveolin-1 scaffolding domain peptide negative control (Neg, a scrambled version of the caveolin-1 scaffolding domain peptide), 125 µM CaCl2 [+ 0.1% DMSO (Ca)], 125 µM CaCl2 + 3 µM cell-permeable caveolin-1 scaffolding domain peptide (Ca + CSDP), and 125 µM CaCl2 + 3 µM cell-permeable caveolin-1 scaffolding domain peptide negative control (Ca + Neg).

    Techniques: Scaffolding, Negative Control

    The caveolin-1 scaffolding domain peptide prevents the calcium-induced inhibition of DNA synthesis. Keratinocytes were treated for 24 hours with medium containing vehicle (0.1% DMS0) or 3 µM caveolin-1 scaffolding domain peptide (CSDP) or the negative control (Neg) in medium containing 25 µM calcium (Con) or 125 µM calcium (Ca 2+ ), as indicated. [ 3 H]Thymidine incorporation into DNA was measured as described in the Materials and Methods. Values are expressed as the percent control and represent the means ± SEM of 12 separate experiments performed in duplicate; *p

    Journal: PLoS ONE

    Article Title: The Caveolin-1 Scaffolding Domain Peptide Decreases Phosphatidylglycerol Levels and Inhibits Calcium-Induced Differentiation in Mouse Keratinocytes

    doi: 10.1371/journal.pone.0080946

    Figure Lengend Snippet: The caveolin-1 scaffolding domain peptide prevents the calcium-induced inhibition of DNA synthesis. Keratinocytes were treated for 24 hours with medium containing vehicle (0.1% DMS0) or 3 µM caveolin-1 scaffolding domain peptide (CSDP) or the negative control (Neg) in medium containing 25 µM calcium (Con) or 125 µM calcium (Ca 2+ ), as indicated. [ 3 H]Thymidine incorporation into DNA was measured as described in the Materials and Methods. Values are expressed as the percent control and represent the means ± SEM of 12 separate experiments performed in duplicate; *p

    Article Snippet: Measurement of DNA Synthesis Near-confluent cultures were refed with SFKM containing 0.1% DMSO as the control (Con), 3 µM cell-permeable caveolin-1 scaffolding domain peptide (Calbiochem, Darmstadt, Germany), 3 µM cell-permeable caveolin-1 scaffolding domain peptide negative control (Neg, a scrambled version of the caveolin-1 scaffolding domain peptide), 125 µM CaCl2 [+ 0.1% DMSO (Ca)], 125 µM CaCl2 + 3 µM cell-permeable caveolin-1 scaffolding domain peptide (Ca + CSDP), and 125 µM CaCl2 + 3 µM cell-permeable caveolin-1 scaffolding domain peptide negative control (Ca + Neg).

    Techniques: Scaffolding, Inhibition, DNA Synthesis, Negative Control

    The caveolin-1 scaffolding domain peptide prevents the calcium-induced stimulation of transglutaminase activity. Keratinocytes were treated for 24 hours with SFKM containing vehicle (0.1% DMSO) or 3 µM caveolin-1 scaffolding domain peptide (CSDP) or the negative control (Neg) in medium containing 25 µM calcium (Con) or 125 µM calcium (Ca 2+ ), as indicated. Transglutaminase activity was then measured as described in Materials and Methods. Values are expressed as the percent control and represent the means ± SEM of 6 separate experiments performed in duplicate; *p

    Journal: PLoS ONE

    Article Title: The Caveolin-1 Scaffolding Domain Peptide Decreases Phosphatidylglycerol Levels and Inhibits Calcium-Induced Differentiation in Mouse Keratinocytes

    doi: 10.1371/journal.pone.0080946

    Figure Lengend Snippet: The caveolin-1 scaffolding domain peptide prevents the calcium-induced stimulation of transglutaminase activity. Keratinocytes were treated for 24 hours with SFKM containing vehicle (0.1% DMSO) or 3 µM caveolin-1 scaffolding domain peptide (CSDP) or the negative control (Neg) in medium containing 25 µM calcium (Con) or 125 µM calcium (Ca 2+ ), as indicated. Transglutaminase activity was then measured as described in Materials and Methods. Values are expressed as the percent control and represent the means ± SEM of 6 separate experiments performed in duplicate; *p

    Article Snippet: Measurement of DNA Synthesis Near-confluent cultures were refed with SFKM containing 0.1% DMSO as the control (Con), 3 µM cell-permeable caveolin-1 scaffolding domain peptide (Calbiochem, Darmstadt, Germany), 3 µM cell-permeable caveolin-1 scaffolding domain peptide negative control (Neg, a scrambled version of the caveolin-1 scaffolding domain peptide), 125 µM CaCl2 [+ 0.1% DMSO (Ca)], 125 µM CaCl2 + 3 µM cell-permeable caveolin-1 scaffolding domain peptide (Ca + CSDP), and 125 µM CaCl2 + 3 µM cell-permeable caveolin-1 scaffolding domain peptide negative control (Ca + Neg).

    Techniques: Scaffolding, Activity Assay, Negative Control

    Caveolin-1 scaffolding domain peptide pretreatment has no effect on calcium-induced IP 3 production. Keratinocytes were pretreated for 24 hours with SFKM containing vehicle (0.1% DMS0) or 3 µM caveolin-1 scaffolding domain peptide (CSDP). The cells were then treated for 10 minutes with control (25 µM calcium-containing) medium or 1 mM calcium-containing medium (to trigger immediate and maximal calcium-sensing receptor activation), and inositol 1,4,5-trisphosphate levels were measured with a radioreceptor assay as described in Materials and Methods. Values are expressed as the percent control and represent the means ± SEM of 4 separate experiments performed in duplicate; ***p

    Journal: PLoS ONE

    Article Title: The Caveolin-1 Scaffolding Domain Peptide Decreases Phosphatidylglycerol Levels and Inhibits Calcium-Induced Differentiation in Mouse Keratinocytes

    doi: 10.1371/journal.pone.0080946

    Figure Lengend Snippet: Caveolin-1 scaffolding domain peptide pretreatment has no effect on calcium-induced IP 3 production. Keratinocytes were pretreated for 24 hours with SFKM containing vehicle (0.1% DMS0) or 3 µM caveolin-1 scaffolding domain peptide (CSDP). The cells were then treated for 10 minutes with control (25 µM calcium-containing) medium or 1 mM calcium-containing medium (to trigger immediate and maximal calcium-sensing receptor activation), and inositol 1,4,5-trisphosphate levels were measured with a radioreceptor assay as described in Materials and Methods. Values are expressed as the percent control and represent the means ± SEM of 4 separate experiments performed in duplicate; ***p

    Article Snippet: Measurement of DNA Synthesis Near-confluent cultures were refed with SFKM containing 0.1% DMSO as the control (Con), 3 µM cell-permeable caveolin-1 scaffolding domain peptide (Calbiochem, Darmstadt, Germany), 3 µM cell-permeable caveolin-1 scaffolding domain peptide negative control (Neg, a scrambled version of the caveolin-1 scaffolding domain peptide), 125 µM CaCl2 [+ 0.1% DMSO (Ca)], 125 µM CaCl2 + 3 µM cell-permeable caveolin-1 scaffolding domain peptide (Ca + CSDP), and 125 µM CaCl2 + 3 µM cell-permeable caveolin-1 scaffolding domain peptide negative control (Ca + Neg).

    Techniques: Scaffolding, Activation Assay

    The caveolin-1 scaffolding domain peptide has a minimal effect on calcium-induced glycerol uptake. Keratinocytes were treated for 24 hours with SFKM containing vehicle (0.1% DMS0) or 3 µM caveolin-1 scaffolding domain peptide (CSDP) or the negative control (Neg) in medium containing 25 µM calcium (Con) or 125 µM calcium (Ca 2+ ) as indicated. [ 3 H]Glycerol uptake was then measured as described in Materials and Methods. Values are expressed as the percent control and represent the means ± SEM of 4 separate experiments performed in duplicate; **p

    Journal: PLoS ONE

    Article Title: The Caveolin-1 Scaffolding Domain Peptide Decreases Phosphatidylglycerol Levels and Inhibits Calcium-Induced Differentiation in Mouse Keratinocytes

    doi: 10.1371/journal.pone.0080946

    Figure Lengend Snippet: The caveolin-1 scaffolding domain peptide has a minimal effect on calcium-induced glycerol uptake. Keratinocytes were treated for 24 hours with SFKM containing vehicle (0.1% DMS0) or 3 µM caveolin-1 scaffolding domain peptide (CSDP) or the negative control (Neg) in medium containing 25 µM calcium (Con) or 125 µM calcium (Ca 2+ ) as indicated. [ 3 H]Glycerol uptake was then measured as described in Materials and Methods. Values are expressed as the percent control and represent the means ± SEM of 4 separate experiments performed in duplicate; **p

    Article Snippet: Measurement of DNA Synthesis Near-confluent cultures were refed with SFKM containing 0.1% DMSO as the control (Con), 3 µM cell-permeable caveolin-1 scaffolding domain peptide (Calbiochem, Darmstadt, Germany), 3 µM cell-permeable caveolin-1 scaffolding domain peptide negative control (Neg, a scrambled version of the caveolin-1 scaffolding domain peptide), 125 µM CaCl2 [+ 0.1% DMSO (Ca)], 125 µM CaCl2 + 3 µM cell-permeable caveolin-1 scaffolding domain peptide (Ca + CSDP), and 125 µM CaCl2 + 3 µM cell-permeable caveolin-1 scaffolding domain peptide negative control (Ca + Neg).

    Techniques: Scaffolding, Negative Control

    The caveolin-1 scaffolding domain peptide decreases phosphatidylglycerol levels in calcium-pretreated keratinocytes. Keratinocytes were pretreated for 24 hours with SFKM containing 125 µM calcium prior to treatment for the indicated times with vehicle (DMSO) or 3µM caveolin-1 scaffolding domain peptide (CSDP) or the negative control (Neg) as indicated. [ 14 C]Phosphatidylglycerol levels were then measured as described in the Materials and Methods. Values are expressed as the percent vehicle and represent the means ± SEM of 3 separate experiments performed in duplicate; **p

    Journal: PLoS ONE

    Article Title: The Caveolin-1 Scaffolding Domain Peptide Decreases Phosphatidylglycerol Levels and Inhibits Calcium-Induced Differentiation in Mouse Keratinocytes

    doi: 10.1371/journal.pone.0080946

    Figure Lengend Snippet: The caveolin-1 scaffolding domain peptide decreases phosphatidylglycerol levels in calcium-pretreated keratinocytes. Keratinocytes were pretreated for 24 hours with SFKM containing 125 µM calcium prior to treatment for the indicated times with vehicle (DMSO) or 3µM caveolin-1 scaffolding domain peptide (CSDP) or the negative control (Neg) as indicated. [ 14 C]Phosphatidylglycerol levels were then measured as described in the Materials and Methods. Values are expressed as the percent vehicle and represent the means ± SEM of 3 separate experiments performed in duplicate; **p

    Article Snippet: Measurement of DNA Synthesis Near-confluent cultures were refed with SFKM containing 0.1% DMSO as the control (Con), 3 µM cell-permeable caveolin-1 scaffolding domain peptide (Calbiochem, Darmstadt, Germany), 3 µM cell-permeable caveolin-1 scaffolding domain peptide negative control (Neg, a scrambled version of the caveolin-1 scaffolding domain peptide), 125 µM CaCl2 [+ 0.1% DMSO (Ca)], 125 µM CaCl2 + 3 µM cell-permeable caveolin-1 scaffolding domain peptide (Ca + CSDP), and 125 µM CaCl2 + 3 µM cell-permeable caveolin-1 scaffolding domain peptide negative control (Ca + Neg).

    Techniques: Scaffolding, Negative Control

    Clathrin and caveolin-1 are expressed in cultured podocytes. A : Western blot analysis shows both clathrin and caveolin-1 are expressed in cultured podocytes. B : in podocytes fixed after 10 min of incubation in FITC-albumin (green), immunostaining of clathrin

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Human podocytes perform polarized, caveolae-dependent albumin endocytosis

    doi: 10.1152/ajprenal.00532.2013

    Figure Lengend Snippet: Clathrin and caveolin-1 are expressed in cultured podocytes. A : Western blot analysis shows both clathrin and caveolin-1 are expressed in cultured podocytes. B : in podocytes fixed after 10 min of incubation in FITC-albumin (green), immunostaining of clathrin

    Article Snippet: Caveolin-mediated endocytosis was inhibited by treating cells with 20 μg/ml nystatin (Calbiochem, Gibbstown, NJ; Ref. ).

    Techniques: Cell Culture, Western Blot, Incubation, Immunostaining

    Podocytes in vivo express clathrin and caveolin-1. A : immunostaining of clathrin (blue) in rat renal glomeruli shows specific staining in podocytes. Podocin staining (green) highlights podocytes, and phalloidin staining of F-actin (red) highlights the

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Human podocytes perform polarized, caveolae-dependent albumin endocytosis

    doi: 10.1152/ajprenal.00532.2013

    Figure Lengend Snippet: Podocytes in vivo express clathrin and caveolin-1. A : immunostaining of clathrin (blue) in rat renal glomeruli shows specific staining in podocytes. Podocin staining (green) highlights podocytes, and phalloidin staining of F-actin (red) highlights the

    Article Snippet: Caveolin-mediated endocytosis was inhibited by treating cells with 20 μg/ml nystatin (Calbiochem, Gibbstown, NJ; Ref. ).

    Techniques: In Vivo, Immunostaining, Staining

    Lipid raft preparations demonstrating increased the presence of p-PKC α , phosphorylated and total caveolin-1 (p-Cav-1 and t-Cav-1, respectively) and phosphorylated cSrc (p-cSrc) in the same raft fractions 1 h after viral infection (V) as compared to mock infection (M), and the effect of chemical inhibition of cSrc by PP2 (A) and (B), and of PKC α by calphostin C (Cal.C) in (C). (A) Western blot for p-PKC α shows increased signal upon viral infection, with slight reduction upon PP2 treatment (10 µ M, or DMSO control). (B) Western blot for p-Cav-1, with or without pretreatment by PP2 (or DMSO control), stripped and reprobed for p-cSrc and then stripped and reprobed for t-Cav-1, shows increased p-Cav-1 and p-cSrc in the same lipid raft fractions with a reduction in phosphorylation for both in cells pretreated with PP2. t-Cav-1 in the lipid raft fractions was also slightly reduced by PP2 pretreatment. (C) Western blots on cells pretreated with either DMSO control or calphostin C (1 µ M) for 3 h prior to mock or virus infection, and stripped and reprobed as in (B), show a dramatic reduction in p-Cav-1 but not t-Cav-1, and complete abrogation of p-cSrc expression by calphostin C in the same lipid raft fractions.

    Journal: Biochemistry

    Article Title: Protein Kinase C Signaling in Adenoviral Infection

    doi: 10.1021/acs.biochem.6b00858

    Figure Lengend Snippet: Lipid raft preparations demonstrating increased the presence of p-PKC α , phosphorylated and total caveolin-1 (p-Cav-1 and t-Cav-1, respectively) and phosphorylated cSrc (p-cSrc) in the same raft fractions 1 h after viral infection (V) as compared to mock infection (M), and the effect of chemical inhibition of cSrc by PP2 (A) and (B), and of PKC α by calphostin C (Cal.C) in (C). (A) Western blot for p-PKC α shows increased signal upon viral infection, with slight reduction upon PP2 treatment (10 µ M, or DMSO control). (B) Western blot for p-Cav-1, with or without pretreatment by PP2 (or DMSO control), stripped and reprobed for p-cSrc and then stripped and reprobed for t-Cav-1, shows increased p-Cav-1 and p-cSrc in the same lipid raft fractions with a reduction in phosphorylation for both in cells pretreated with PP2. t-Cav-1 in the lipid raft fractions was also slightly reduced by PP2 pretreatment. (C) Western blots on cells pretreated with either DMSO control or calphostin C (1 µ M) for 3 h prior to mock or virus infection, and stripped and reprobed as in (B), show a dramatic reduction in p-Cav-1 but not t-Cav-1, and complete abrogation of p-cSrc expression by calphostin C in the same lipid raft fractions.

    Article Snippet: Antibodies to total cSrc and phospho-caveolin-1 were purchased from EMD Millipore (Billerica, MA) and BD Bioscience (San Jose, CA).

    Techniques: Infection, Inhibition, Western Blot, Expressing

    Virus infection induces physical association of phosphorylated PKC α (p-PKC α ) with caveolin-1 (Cav-1) in whole corneal fibroblast lysates by immunoprecipitation assays (A) and (B), and confocal microscopy (C). Immunoprecipitation (IP) with anticaveolin-1 (A), or anti-p-PKC α (B) followed by immunoblotting (IB) shows increased p-PKC α in caveolin-1 immunoprecipitates and increased caveolin-1 in p-PKC α immunoprecipitates, respectively, at 30 min after viral infection (V), as compared to mock infection (M) with virus-free dialysis buffer. Antirabbit and antimouse IgG isotype controls did not immunoprecipitate either protein. Immunoconfocal microscopy (C) at 30 min post infection demonstrates mostly membrane bound p-PKC α (Alexa Fluor 488: green) and caveolin-1 (Cav-1, Alexa Fluor 568: red) in mock infected cells (M). Virus infected cells (V) show intracellular colocalization of p-PKC α and caveolin-1 in intracytoplasmic vesicles. Cell nuclei are stained with DAPI (blue). Original magnification: 63×.

    Journal: Biochemistry

    Article Title: Protein Kinase C Signaling in Adenoviral Infection

    doi: 10.1021/acs.biochem.6b00858

    Figure Lengend Snippet: Virus infection induces physical association of phosphorylated PKC α (p-PKC α ) with caveolin-1 (Cav-1) in whole corneal fibroblast lysates by immunoprecipitation assays (A) and (B), and confocal microscopy (C). Immunoprecipitation (IP) with anticaveolin-1 (A), or anti-p-PKC α (B) followed by immunoblotting (IB) shows increased p-PKC α in caveolin-1 immunoprecipitates and increased caveolin-1 in p-PKC α immunoprecipitates, respectively, at 30 min after viral infection (V), as compared to mock infection (M) with virus-free dialysis buffer. Antirabbit and antimouse IgG isotype controls did not immunoprecipitate either protein. Immunoconfocal microscopy (C) at 30 min post infection demonstrates mostly membrane bound p-PKC α (Alexa Fluor 488: green) and caveolin-1 (Cav-1, Alexa Fluor 568: red) in mock infected cells (M). Virus infected cells (V) show intracellular colocalization of p-PKC α and caveolin-1 in intracytoplasmic vesicles. Cell nuclei are stained with DAPI (blue). Original magnification: 63×.

    Article Snippet: Antibodies to total cSrc and phospho-caveolin-1 were purchased from EMD Millipore (Billerica, MA) and BD Bioscience (San Jose, CA).

    Techniques: Infection, Immunoprecipitation, Confocal Microscopy, Microscopy, Staining

    Activation of cSrc and caveolin-1 in endosomes occurs downstream of PKC α . (A) Western blot analysis of endosomal preparations of serum starved corneal fibroblasts mock infected (M), DMSO (V), or calphostin C pretreated and virus infected (Cal.C+V) for two hr. Postnuclear extracts were subjected to endosome purification using sucrose gradient centrifugation. After SDS-PAGE, membranes were blotted with antibody to p-PKC α , then stripped and reprobed with antibody against phosphorylated cSrc (p-cSrc), and then stripped and reprobed with antibody to phosphorylated caveolin-1 (p-Cav-1). Virus infection increased expression of all three proteins, and all were markedly reduced by pretreatment with calphostin C. (B) cSrc kinase assay performed at 2 h post infection on pooled endosomal preparation fractions 7 through 10 shows increased cSrc activity in virus infection (V) than in mock infection (M) or in virus infected cells pretreated with calphostin C (Cal.C + V, * p

    Journal: Biochemistry

    Article Title: Protein Kinase C Signaling in Adenoviral Infection

    doi: 10.1021/acs.biochem.6b00858

    Figure Lengend Snippet: Activation of cSrc and caveolin-1 in endosomes occurs downstream of PKC α . (A) Western blot analysis of endosomal preparations of serum starved corneal fibroblasts mock infected (M), DMSO (V), or calphostin C pretreated and virus infected (Cal.C+V) for two hr. Postnuclear extracts were subjected to endosome purification using sucrose gradient centrifugation. After SDS-PAGE, membranes were blotted with antibody to p-PKC α , then stripped and reprobed with antibody against phosphorylated cSrc (p-cSrc), and then stripped and reprobed with antibody to phosphorylated caveolin-1 (p-Cav-1). Virus infection increased expression of all three proteins, and all were markedly reduced by pretreatment with calphostin C. (B) cSrc kinase assay performed at 2 h post infection on pooled endosomal preparation fractions 7 through 10 shows increased cSrc activity in virus infection (V) than in mock infection (M) or in virus infected cells pretreated with calphostin C (Cal.C + V, * p

    Article Snippet: Antibodies to total cSrc and phospho-caveolin-1 were purchased from EMD Millipore (Billerica, MA) and BD Bioscience (San Jose, CA).

    Techniques: Activation Assay, Western Blot, Infection, Purification, Gradient Centrifugation, SDS Page, Expressing, Kinase Assay, Activity Assay