cationic lipid transfection reagent transfast  (Promega)

 
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    Name:
    TransFast Transfection Reagent
    Description:
    Cationic lipid based high efficiency transfection of eukaryotic cells
    Catalog Number:
    e2431
    Price:
    None
    Category:
    Cell Biology Reporter Assays Transfection Transfection Reagents
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    Structured Review

    Promega cationic lipid transfection reagent transfast
    Cationic lipid based high efficiency transfection of eukaryotic cells
    https://www.bioz.com/result/cationic lipid transfection reagent transfast/product/Promega
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    cationic lipid transfection reagent transfast - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Electroporation:

    Article Title: Modulation of both activator protein-1 and nuclear factor-kappa B signal transduction of human T cells by amiodarone
    Article Snippet: .. We used the transfection reagent TransFast™ (Promega) to transfect plasmids into the cells instead of using electroporation. .. Briefly, on the day of the transfection, Jurkat T cells were adjusted to 1 × 106 mL−1 in serum-free media and evenly mixed with 2 mg of reporter plasmid (Stratagene, La Jolla, CA) and TransFast™ transfectant (6 mL) in triplicate.

    Transfection:

    Article Title: Modulation of both activator protein-1 and nuclear factor-kappa B signal transduction of human T cells by amiodarone
    Article Snippet: .. We used the transfection reagent TransFast™ (Promega) to transfect plasmids into the cells instead of using electroporation. .. Briefly, on the day of the transfection, Jurkat T cells were adjusted to 1 × 106 mL−1 in serum-free media and evenly mixed with 2 mg of reporter plasmid (Stratagene, La Jolla, CA) and TransFast™ transfectant (6 mL) in triplicate.

    Article Title: Transcriptional regulation of matrix metalloproteinase-1 and collagen 1A2 explains the anti-fibrotic effect exerted by proteasome inhibition in human dermal fibroblasts
    Article Snippet: .. Various combinations of plasmid DNA (1 μg total) and 3 μL of transfection reagent (Transfast from Promega Corporation) were added to 1 mL of medium containing 1% FCS and mixed vigorously. .. The transfection mix contained Renilla luciferase and firefly luciferase plasmids at a ratio of 1 to 10.

    Article Title: Charge-reversal Lipids, Peptide-based Lipids, and Nucleoside-based Lipids for Gene Delivery
    Article Snippet: .. The transfection efficiency of 1 was comparable to the commercial transfection reagent TransFast (Promega), a cationic lipid-based gene delivery vector. ..

    Article Title: Poly(amidoamine) Dendrimer Nanocarriers and Their Aerosol Formulations for siRNA Delivery to the Lung Epithelium
    Article Snippet: .. CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay (MTS reagent powder) and TransFast Transfection Reagent were purchased from Promega Corporation (Madison, WI, U.S.A.). .. Phenazine methosulfate (PMS, 95%) was purchased from MP Biomedicals (Santa Ana, CA, U.S.A.). eGFP lentiviral particles (LVP-340, 1 × 107 IFU × mL–1 , a puromycin gene under Rsv promoter allows the selection of transduced fluorescent positive cells) were purchased from GenTarget Inc. (San Diego, CA, U.S.A.).

    Article Title: Epidermal Growth Factor Triggers an Original, Caspase-independent Pituitary Cell Death with Heterogeneous Phenotype
    Article Snippet: .. The transient GH4C1 cell transfection was performed by using TransFast reagent (Promega, Madison, WI). ..

    Article Title: Charge-reversal Lipids, Peptide-based Lipids, and Nucleoside-based Lipids for Gene Delivery
    Article Snippet: .. O-ethyl-dioleylphosphatidylcholinium-uridine ( O-Et-DOUPC ) was tested for transfection efficiency using a β-gal reporter assay and was shown to be as effective as the commercial transfection reagent TransFast, and more efficient than DOTAP ( ). ..

    Article Title: Inhibition of Methyltransferases Results in Induction of G2/M Checkpoint and Programmed Cell Death in Human T-Lymphotropic Virus Type 1-Transformed Cells ▿
    Article Snippet: .. HTLV-1-transformed cells were transfected with 1 μg of reporter constructs HTLV-1LTR-Luc, PG13-Luc, and NF-κB-Luc (kindly provided by Warner Green) using Transfast transfection reagent (Promega) according to the manufacturer's protocol. .. All transfections included 0.5 μg Rous sarcoma virus (RSV) β-galactosidase (β-Gal) plasmid as a control for transfection efficiency.

    Amplification:

    Article Title: Patterned transgene expression in multiple channel bridges after spinal cord injury
    Article Snippet: .. Plasmids were amplified in Escherichia coli DH5α, purified with Qiagen (Santa Clara, CA) reagents, and complexed with Transfast (Promega, Madison, WI) in a w/v ratio of 1:1.5. .. After incubation for 15 minutes, 10 µL of fibronectin (2 µg/µL) (Sigma-Aldrich, St. Louis, MO) was added to the complexes and pipetted into the channels of the disks that were deposited in 6 well plates.

    Reporter Assay:

    Article Title: Charge-reversal Lipids, Peptide-based Lipids, and Nucleoside-based Lipids for Gene Delivery
    Article Snippet: .. O-ethyl-dioleylphosphatidylcholinium-uridine ( O-Et-DOUPC ) was tested for transfection efficiency using a β-gal reporter assay and was shown to be as effective as the commercial transfection reagent TransFast, and more efficient than DOTAP ( ). ..

    Construct:

    Article Title: Inhibition of Methyltransferases Results in Induction of G2/M Checkpoint and Programmed Cell Death in Human T-Lymphotropic Virus Type 1-Transformed Cells ▿
    Article Snippet: .. HTLV-1-transformed cells were transfected with 1 μg of reporter constructs HTLV-1LTR-Luc, PG13-Luc, and NF-κB-Luc (kindly provided by Warner Green) using Transfast transfection reagent (Promega) according to the manufacturer's protocol. .. All transfections included 0.5 μg Rous sarcoma virus (RSV) β-galactosidase (β-Gal) plasmid as a control for transfection efficiency.

    Purification:

    Article Title: Patterned transgene expression in multiple channel bridges after spinal cord injury
    Article Snippet: .. Plasmids were amplified in Escherichia coli DH5α, purified with Qiagen (Santa Clara, CA) reagents, and complexed with Transfast (Promega, Madison, WI) in a w/v ratio of 1:1.5. .. After incubation for 15 minutes, 10 µL of fibronectin (2 µg/µL) (Sigma-Aldrich, St. Louis, MO) was added to the complexes and pipetted into the channels of the disks that were deposited in 6 well plates.

    Proliferation Assay:

    Article Title: Poly(amidoamine) Dendrimer Nanocarriers and Their Aerosol Formulations for siRNA Delivery to the Lung Epithelium
    Article Snippet: .. CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay (MTS reagent powder) and TransFast Transfection Reagent were purchased from Promega Corporation (Madison, WI, U.S.A.). .. Phenazine methosulfate (PMS, 95%) was purchased from MP Biomedicals (Santa Ana, CA, U.S.A.). eGFP lentiviral particles (LVP-340, 1 × 107 IFU × mL–1 , a puromycin gene under Rsv promoter allows the selection of transduced fluorescent positive cells) were purchased from GenTarget Inc. (San Diego, CA, U.S.A.).

    Plasmid Preparation:

    Article Title: Transcriptional regulation of matrix metalloproteinase-1 and collagen 1A2 explains the anti-fibrotic effect exerted by proteasome inhibition in human dermal fibroblasts
    Article Snippet: .. Various combinations of plasmid DNA (1 μg total) and 3 μL of transfection reagent (Transfast from Promega Corporation) were added to 1 mL of medium containing 1% FCS and mixed vigorously. .. The transfection mix contained Renilla luciferase and firefly luciferase plasmids at a ratio of 1 to 10.

    Article Title: Charge-reversal Lipids, Peptide-based Lipids, and Nucleoside-based Lipids for Gene Delivery
    Article Snippet: .. The transfection efficiency of 1 was comparable to the commercial transfection reagent TransFast (Promega), a cationic lipid-based gene delivery vector. ..

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  • 99
    Promega cationic lipid transfection reagent transfast
    Cationic Lipid Transfection Reagent Transfast, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cationic lipid transfection reagent transfast/product/Promega
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    cationic lipid transfection reagent transfast - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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