caspase 3 primary antibody  (Boster Bio)


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    Boster Bio caspase 3 primary antibody
    Immunohistochemical analysis of <t>caspase-3</t> expression of the apoptotic hepatocyte (magnification, ×400). (A) Untreated control mouse; (B) d -galactosamine ( d -GalN)/lipopolysaccharide (LPS)-treated mouse showing the positive expression of caspase-3 (brown stain).
    Caspase 3 Primary Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caspase 3 primary antibody/product/Boster Bio
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    caspase 3 primary antibody - by Bioz Stars, 2022-09
    88/100 stars

    Images

    1) Product Images from "Nature and mechanisms of hepatocyte apoptosis induced by d-galactosamine/lipopolysaccharide challenge in mice"

    Article Title: Nature and mechanisms of hepatocyte apoptosis induced by d-galactosamine/lipopolysaccharide challenge in mice

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2014.1730

    Immunohistochemical analysis of caspase-3 expression of the apoptotic hepatocyte (magnification, ×400). (A) Untreated control mouse; (B) d -galactosamine ( d -GalN)/lipopolysaccharide (LPS)-treated mouse showing the positive expression of caspase-3 (brown stain).
    Figure Legend Snippet: Immunohistochemical analysis of caspase-3 expression of the apoptotic hepatocyte (magnification, ×400). (A) Untreated control mouse; (B) d -galactosamine ( d -GalN)/lipopolysaccharide (LPS)-treated mouse showing the positive expression of caspase-3 (brown stain).

    Techniques Used: Immunohistochemistry, Expressing, Staining

    Western blot analysis of Fas, Fas ligand (FasL) and caspase-3 expression in liver tissues. The GAPDH antibody was used as internal control. Sample 1 from the untreated control mice; Samples 2–8 from the mice at 8 h after the d -galactosamine ( d -GalN)/lipopolysaccharide (LPS) injection.
    Figure Legend Snippet: Western blot analysis of Fas, Fas ligand (FasL) and caspase-3 expression in liver tissues. The GAPDH antibody was used as internal control. Sample 1 from the untreated control mice; Samples 2–8 from the mice at 8 h after the d -galactosamine ( d -GalN)/lipopolysaccharide (LPS) injection.

    Techniques Used: Western Blot, Expressing, Mouse Assay, Injection

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    Boster Bio caspase 3 primary antibody
    Immunohistochemical analysis of <t>caspase-3</t> expression of the apoptotic hepatocyte (magnification, ×400). (A) Untreated control mouse; (B) d -galactosamine ( d -GalN)/lipopolysaccharide (LPS)-treated mouse showing the positive expression of caspase-3 (brown stain).
    Caspase 3 Primary Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caspase 3 primary antibody/product/Boster Bio
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    caspase 3 primary antibody - by Bioz Stars, 2022-09
    88/100 stars
      Buy from Supplier

    94
    Boster Bio cleaved caspase 3
    Cell apoptosis was decreased by HBV-associated exosomes in a concentration-dependent manner. (A) Cells were treated with 1×10 10 , 2×10 10 or 4×10 10 HBV-associated exosomes, and the expression of cleaved <t>caspase-3</t> and Bcl-2 was assessed by western blotting. (B) Flow cytometry results indicated that cell apoptosis was negatively associated with the concentration of HBV-associated exosomes. HBV, hepatitis B virus; Bcl-2, B-cell lymphoma; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Exo, exosome; Oxa, oxaliplatin; UR, upper right; LR, lower right; Ctr, control; PBS, phosphate-buffered saline.
    Cleaved Caspase 3, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 3/product/Boster Bio
    Average 94 stars, based on 1 article reviews
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    92
    Boster Bio antibodies cdk1
    Effects of SNHG12 expression on the expression levels of miR-148a and <t>CDK1.</t> a QRT-PCR was used to determine miR-148a expression in CC tissues and adjacent normal tissues. b The expression of miR-148a in CC cells (SiHa and Hela) and Ect1/E6E7 cells was detected by qRT-PCR. c The protein level of CDK1 in CC cells (SiHa and Hela) and Ect1/E6E7 cells was measured by WB analysis. d , e The expression of miR-148a was determined by qRT-PCR in SiHa and Hela cells treated with SNHG12 overexpression plasmid and miR-148a mimic or si-SNHG12 and anti-miR-148a. f , g WB analysis was used to detect the protein level of CDK1 in SiHa and Hela cells treated with SNHG12 overexpression plasmid and miR-148a mimic or si-SNHG12 and anti-miR-148a. * P
    Antibodies Cdk1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies cdk1/product/Boster Bio
    Average 92 stars, based on 1 article reviews
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    Boster Bio anti caspase 3
    The expressions of <t>caspase-3</t> in the eyelid shown with immunochemical staining. A – Negative caspase-3 expression in basal cell carcinoma, B – grade 1 caspase-3 expression in basal cell carcinoma, C – grade 3 caspase-3 expression in controls
    Anti Caspase 3, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti caspase 3/product/Boster Bio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti caspase 3 - by Bioz Stars, 2022-09
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    Image Search Results


    Immunohistochemical analysis of caspase-3 expression of the apoptotic hepatocyte (magnification, ×400). (A) Untreated control mouse; (B) d -galactosamine ( d -GalN)/lipopolysaccharide (LPS)-treated mouse showing the positive expression of caspase-3 (brown stain).

    Journal: International Journal of Molecular Medicine

    Article Title: Nature and mechanisms of hepatocyte apoptosis induced by d-galactosamine/lipopolysaccharide challenge in mice

    doi: 10.3892/ijmm.2014.1730

    Figure Lengend Snippet: Immunohistochemical analysis of caspase-3 expression of the apoptotic hepatocyte (magnification, ×400). (A) Untreated control mouse; (B) d -galactosamine ( d -GalN)/lipopolysaccharide (LPS)-treated mouse showing the positive expression of caspase-3 (brown stain).

    Article Snippet: Caspase-3 primary antibody, Fas/Fas ligand (FasL) primary antibodies, mouse tumor necrosis factor-α (TNF-α) enzyme immunoassay kit and mouse transforming growth factor-β1 (TGF-β) enzyme immunoassay kit were purchased from Boster Biological Technology, Ltd. (Wuhan, China).

    Techniques: Immunohistochemistry, Expressing, Staining

    Western blot analysis of Fas, Fas ligand (FasL) and caspase-3 expression in liver tissues. The GAPDH antibody was used as internal control. Sample 1 from the untreated control mice; Samples 2–8 from the mice at 8 h after the d -galactosamine ( d -GalN)/lipopolysaccharide (LPS) injection.

    Journal: International Journal of Molecular Medicine

    Article Title: Nature and mechanisms of hepatocyte apoptosis induced by d-galactosamine/lipopolysaccharide challenge in mice

    doi: 10.3892/ijmm.2014.1730

    Figure Lengend Snippet: Western blot analysis of Fas, Fas ligand (FasL) and caspase-3 expression in liver tissues. The GAPDH antibody was used as internal control. Sample 1 from the untreated control mice; Samples 2–8 from the mice at 8 h after the d -galactosamine ( d -GalN)/lipopolysaccharide (LPS) injection.

    Article Snippet: Caspase-3 primary antibody, Fas/Fas ligand (FasL) primary antibodies, mouse tumor necrosis factor-α (TNF-α) enzyme immunoassay kit and mouse transforming growth factor-β1 (TGF-β) enzyme immunoassay kit were purchased from Boster Biological Technology, Ltd. (Wuhan, China).

    Techniques: Western Blot, Expressing, Mouse Assay, Injection

    Cell apoptosis was decreased by HBV-associated exosomes in a concentration-dependent manner. (A) Cells were treated with 1×10 10 , 2×10 10 or 4×10 10 HBV-associated exosomes, and the expression of cleaved caspase-3 and Bcl-2 was assessed by western blotting. (B) Flow cytometry results indicated that cell apoptosis was negatively associated with the concentration of HBV-associated exosomes. HBV, hepatitis B virus; Bcl-2, B-cell lymphoma; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Exo, exosome; Oxa, oxaliplatin; UR, upper right; LR, lower right; Ctr, control; PBS, phosphate-buffered saline.

    Journal: Oncology Letters

    Article Title: Exosomes derived from HBV-associated liver cancer promote chemoresistance by upregulating chaperone-mediated autophagy

    doi: 10.3892/ol.2018.9584

    Figure Lengend Snippet: Cell apoptosis was decreased by HBV-associated exosomes in a concentration-dependent manner. (A) Cells were treated with 1×10 10 , 2×10 10 or 4×10 10 HBV-associated exosomes, and the expression of cleaved caspase-3 and Bcl-2 was assessed by western blotting. (B) Flow cytometry results indicated that cell apoptosis was negatively associated with the concentration of HBV-associated exosomes. HBV, hepatitis B virus; Bcl-2, B-cell lymphoma; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Exo, exosome; Oxa, oxaliplatin; UR, upper right; LR, lower right; Ctr, control; PBS, phosphate-buffered saline.

    Article Snippet: The primary antibody against cleaved caspase-3 (dilution, 1:500; catalog no. PB0183) was obtained from Wuhan Boster Biological Technology, Ltd. (Wuhan, China), the anti-B-cell lymphoma-2 (Bcl-2) antibody (dilution, 1:500; catalog no. WL01556) was obtained from Wanleibio Co., Ltd. (Shanghai, China) and the antibody against GAPDH (dilution, 1:5,000; catalog no. 5174S) was obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA).

    Techniques: Concentration Assay, Expressing, Western Blot, Flow Cytometry, Cytometry

    Effects of SNHG12 expression on the expression levels of miR-148a and CDK1. a QRT-PCR was used to determine miR-148a expression in CC tissues and adjacent normal tissues. b The expression of miR-148a in CC cells (SiHa and Hela) and Ect1/E6E7 cells was detected by qRT-PCR. c The protein level of CDK1 in CC cells (SiHa and Hela) and Ect1/E6E7 cells was measured by WB analysis. d , e The expression of miR-148a was determined by qRT-PCR in SiHa and Hela cells treated with SNHG12 overexpression plasmid and miR-148a mimic or si-SNHG12 and anti-miR-148a. f , g WB analysis was used to detect the protein level of CDK1 in SiHa and Hela cells treated with SNHG12 overexpression plasmid and miR-148a mimic or si-SNHG12 and anti-miR-148a. * P

    Journal: Cancer Cell International

    Article Title: LncRNA SNHG12 regulates the radiosensitivity of cervical cancer through the miR-148a/CDK1 pathway

    doi: 10.1186/s12935-020-01654-5

    Figure Lengend Snippet: Effects of SNHG12 expression on the expression levels of miR-148a and CDK1. a QRT-PCR was used to determine miR-148a expression in CC tissues and adjacent normal tissues. b The expression of miR-148a in CC cells (SiHa and Hela) and Ect1/E6E7 cells was detected by qRT-PCR. c The protein level of CDK1 in CC cells (SiHa and Hela) and Ect1/E6E7 cells was measured by WB analysis. d , e The expression of miR-148a was determined by qRT-PCR in SiHa and Hela cells treated with SNHG12 overexpression plasmid and miR-148a mimic or si-SNHG12 and anti-miR-148a. f , g WB analysis was used to detect the protein level of CDK1 in SiHa and Hela cells treated with SNHG12 overexpression plasmid and miR-148a mimic or si-SNHG12 and anti-miR-148a. * P

    Article Snippet: After blocking with non-fat milk for 2 h, the membranes were incubated with primary antibodies CDK1 (1:500, BA0027-2, Boster, Wuhan, China), CCND1 (1:2000, PB0403, Boster), γ-H2AX (1:1000, AF5836, Beyotime) and β-actin (1:5000, BA2305, Boster) overnight at 4 °C, followed by incubating with secondary antibody (1:10,000, BA1056, Boster) for 1 h. Immunoreactive bands were visualized using enhanced chemiluminescence reagent (Millipore).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, Over Expression, Plasmid Preparation

    Effects of miR-148a inhibitor and CDK1 overexpression on radiosensitivity, apoptosis and cell cycle in CC cells. SiHa and Hela cells were transfected with si-SNHG12 and anti-miR-148a or CDK1 overexpression plasmid. a , b The survival fractions of SiHa and Hela cells were assessed by clonogenic assay. c , d Flow cytometry was used to determine the apoptosis of SiHa and Hela cells treated with 2 Gy radiation. e , f Caspase-3 Activity Assay Kit was used to detect the caspase-3 activity of SiHa and Hela cells treated with 2 Gy radiation. g , h The cell cycle distribution in SiHa and Hela cells treated with 2 Gy radiation was determined using flow cytometry. i , j The protein expression of CCND1 in SiHa and Hela cells treated with 2 Gy radiation was tested by WB analysis. * P

    Journal: Cancer Cell International

    Article Title: LncRNA SNHG12 regulates the radiosensitivity of cervical cancer through the miR-148a/CDK1 pathway

    doi: 10.1186/s12935-020-01654-5

    Figure Lengend Snippet: Effects of miR-148a inhibitor and CDK1 overexpression on radiosensitivity, apoptosis and cell cycle in CC cells. SiHa and Hela cells were transfected with si-SNHG12 and anti-miR-148a or CDK1 overexpression plasmid. a , b The survival fractions of SiHa and Hela cells were assessed by clonogenic assay. c , d Flow cytometry was used to determine the apoptosis of SiHa and Hela cells treated with 2 Gy radiation. e , f Caspase-3 Activity Assay Kit was used to detect the caspase-3 activity of SiHa and Hela cells treated with 2 Gy radiation. g , h The cell cycle distribution in SiHa and Hela cells treated with 2 Gy radiation was determined using flow cytometry. i , j The protein expression of CCND1 in SiHa and Hela cells treated with 2 Gy radiation was tested by WB analysis. * P

    Article Snippet: After blocking with non-fat milk for 2 h, the membranes were incubated with primary antibodies CDK1 (1:500, BA0027-2, Boster, Wuhan, China), CCND1 (1:2000, PB0403, Boster), γ-H2AX (1:1000, AF5836, Beyotime) and β-actin (1:5000, BA2305, Boster) overnight at 4 °C, followed by incubating with secondary antibody (1:10,000, BA1056, Boster) for 1 h. Immunoreactive bands were visualized using enhanced chemiluminescence reagent (Millipore).

    Techniques: Over Expression, Transfection, Plasmid Preparation, Clonogenic Assay, Flow Cytometry, Caspase-3 Activity Assay, Activity Assay, Expressing, Western Blot

    Effects of SNHG12 knockdown on the tumor growth of CC in vivo. The CC mice xenograft models were constructed by injecting sh-con or sh-SNHG12 transfected Hela cells and un-transfected Hela cells (Empty) into nude mice. a Tumor volume was calculated at the indicated time point. b Tumor weight was measured in mice. c , d The expression levels of SNHG12 and miR-148a were detected by qRT-PCR. e The protein level of CDK1 was evaluated by WB analysis. f The results of Ki-67 and TUNEL staining were presented. g The pictures of HE staining was exhibited.* P

    Journal: Cancer Cell International

    Article Title: LncRNA SNHG12 regulates the radiosensitivity of cervical cancer through the miR-148a/CDK1 pathway

    doi: 10.1186/s12935-020-01654-5

    Figure Lengend Snippet: Effects of SNHG12 knockdown on the tumor growth of CC in vivo. The CC mice xenograft models were constructed by injecting sh-con or sh-SNHG12 transfected Hela cells and un-transfected Hela cells (Empty) into nude mice. a Tumor volume was calculated at the indicated time point. b Tumor weight was measured in mice. c , d The expression levels of SNHG12 and miR-148a were detected by qRT-PCR. e The protein level of CDK1 was evaluated by WB analysis. f The results of Ki-67 and TUNEL staining were presented. g The pictures of HE staining was exhibited.* P

    Article Snippet: After blocking with non-fat milk for 2 h, the membranes were incubated with primary antibodies CDK1 (1:500, BA0027-2, Boster, Wuhan, China), CCND1 (1:2000, PB0403, Boster), γ-H2AX (1:1000, AF5836, Beyotime) and β-actin (1:5000, BA2305, Boster) overnight at 4 °C, followed by incubating with secondary antibody (1:10,000, BA1056, Boster) for 1 h. Immunoreactive bands were visualized using enhanced chemiluminescence reagent (Millipore).

    Techniques: In Vivo, Mouse Assay, Construct, Transfection, Expressing, Quantitative RT-PCR, Western Blot, TUNEL Assay, Staining

    CDK1 was targeted by miR-148a. a The sequences of miR-148a containing the CDK1 3′UTR binding sites and mutant binding sites were shown. b , c The binding of miR-148a in 3′UTR of CDK1 was verified by dual-luciferase reporter assay. d , e The enrichments of CDK1 and miR-148a in Anti-IgG, Anti-Ago2 and Input were detected by RIP assay. f , g WB analysis was used to detect the effect of miR-148a expression on the protein level of CDK1 in SiHa and Hela cells. * P

    Journal: Cancer Cell International

    Article Title: LncRNA SNHG12 regulates the radiosensitivity of cervical cancer through the miR-148a/CDK1 pathway

    doi: 10.1186/s12935-020-01654-5

    Figure Lengend Snippet: CDK1 was targeted by miR-148a. a The sequences of miR-148a containing the CDK1 3′UTR binding sites and mutant binding sites were shown. b , c The binding of miR-148a in 3′UTR of CDK1 was verified by dual-luciferase reporter assay. d , e The enrichments of CDK1 and miR-148a in Anti-IgG, Anti-Ago2 and Input were detected by RIP assay. f , g WB analysis was used to detect the effect of miR-148a expression on the protein level of CDK1 in SiHa and Hela cells. * P

    Article Snippet: After blocking with non-fat milk for 2 h, the membranes were incubated with primary antibodies CDK1 (1:500, BA0027-2, Boster, Wuhan, China), CCND1 (1:2000, PB0403, Boster), γ-H2AX (1:1000, AF5836, Beyotime) and β-actin (1:5000, BA2305, Boster) overnight at 4 °C, followed by incubating with secondary antibody (1:10,000, BA1056, Boster) for 1 h. Immunoreactive bands were visualized using enhanced chemiluminescence reagent (Millipore).

    Techniques: Binding Assay, Mutagenesis, Luciferase, Reporter Assay, Western Blot, Expressing

    The expressions of caspase-3 in the eyelid shown with immunochemical staining. A – Negative caspase-3 expression in basal cell carcinoma, B – grade 1 caspase-3 expression in basal cell carcinoma, C – grade 3 caspase-3 expression in controls

    Journal: Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii

    Article Title: Caspase-3, p53 and Bcl-2 expression in basal cell carcinoma of the eyelid

    doi: 10.5114/ada.2020.98285

    Figure Lengend Snippet: The expressions of caspase-3 in the eyelid shown with immunochemical staining. A – Negative caspase-3 expression in basal cell carcinoma, B – grade 1 caspase-3 expression in basal cell carcinoma, C – grade 3 caspase-3 expression in controls

    Article Snippet: The sections were then covered with the primary antibodies diluted 1 : 500 for anti-caspase-3, 1 : 200 for anti-p53, 1 : 500 for anti-bcl-2 in TBS at 4°C overnight (Anti-caspase-3 (PA1302-1); anti-p53 (MA1078); anti-bcl-2 (MA1004) were from Boster Biological Technology Co. Ltd., USA).

    Techniques: Staining, Expressing