caspase 3 enzyme activity assay kit  (Beyotime)

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

Article Snippet: According to the instructions of the Caspase-3 Enzyme Activity Assay Kit (Beyotime, Shanghai, China), cells from each group were lysed for 15 min on ice using lysis buffer.

Techniques: Expressing

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

Article Snippet: According to the instructions of the Caspase-3 Enzyme Activity Assay Kit (Beyotime, Shanghai, China), cells from each group were lysed for 15 min on ice using lysis buffer.

Techniques: Activity Assay

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: Effect of Et MIC2 on E.tenella infection rate and Caspase 3 Activity through ITGAV.

Article Snippet: According to the instructions of the Caspase-3 Enzyme Activity Assay Kit (Beyotime, Shanghai, China), cells from each group were lysed for 15 min on ice using lysis buffer.

Techniques: Infection, Activity Assay

Journal: Dental materials : official publication of the Academy of Dental Materials

Article Title: Cytotoxicity effects and differentiation potential of ormocer-based and nanohybrid composite resins on human dental pulp stem cells.

doi: 10.1016/j.dental.2024.05.011

Figure Lengend Snippet: Fig. 1. Cell viability in growth medium of conventional composite Ceram.x (CX) (A) and the innovative technology Ormocer Admira® Fusion (AD) (B) evaluated with MTT assay after 96 h (n = 3); apoptosis evaluation tested with caspase 3/7 activity assay (n = 3) (C). * p < 0.05, * * p < 0.01, * ** p < 0.001, * ** * p < 0.0001.

Article Snippet: hDPSCs (80.000 cells/well in a 24 well-plate) were treated with the dilutions 1:1, 1:50 and 1:100 of CX and AD eluates in growth medium for 96 h. At the end of stimulation, caspase 3/7 enzyme activity (Elabscience, E-CK-A383) was calculated as enzymatic units/mg protein according to manufacturer’s protocols to evaluate the apoptosis.

Techniques: MTT Assay, Activity Assay

Journal: Heliyon

Article Title: Integrating network pharmacology and experimental verification strategies to reveal the active ingredients and molecular mechanism of Tenghuang Jiangu Capsule against osteoporosis

doi: 10.1016/j.heliyon.2023.e19812

Figure Lengend Snippet: Quantitative real-time PCR primer sequences.

Article Snippet: The pNA standard curve was determined according to the Caspase3 enzyme activity assay kit (C1157, Beyotime), and after preparing the sample system according to the kit, the OD value at 405 nm was measured using an enzyme marker.

Techniques: Real-time Polymerase Chain Reaction

Journal: Heliyon

Article Title: Integrating network pharmacology and experimental verification strategies to reveal the active ingredients and molecular mechanism of Tenghuang Jiangu Capsule against osteoporosis

doi: 10.1016/j.heliyon.2023.e19812

Figure Lengend Snippet: Molecular docking verification of the selected ingredients to the key targets. (A) Mode of action of Akt1, Caspase3, MAPK1, MAPK3, MAPK8 interacting with quercetin, luteolin, and kaempferol. (B) The detail binding energy score was displayed with a heatmap.

Article Snippet: The pNA standard curve was determined according to the Caspase3 enzyme activity assay kit (C1157, Beyotime), and after preparing the sample system according to the kit, the OD value at 405 nm was measured using an enzyme marker.

Techniques: Binding Assay

Journal: Heliyon

Article Title: Integrating network pharmacology and experimental verification strategies to reveal the active ingredients and molecular mechanism of Tenghuang Jiangu Capsule against osteoporosis

doi: 10.1016/j.heliyon.2023.e19812

Figure Lengend Snippet: THJGC inhibits H 2 O 2 -induced apoptosis and reactive oxygen species (ROS) production. (A) ROS production was detected with a DCFH-DA probe, and the relative fluorescence intensity of ROS was quantified in Figure (B). Scale bar = 100 μm. (C) Quantitative statistical plot of caspase3 enzyme activity assay. (E) Treatment with H 2 O 2 with or without THJGC intervention for 24hr, Annexin V-FITC/PI apoptosis staining and assayed by flow cytometry. Percentage of apoptosis is shown in Figure (E). (F) Expression of apoptosis-related genes, including Bax, Bcl-2, Caspase3. The sample size of all the above experiments was three (n = 3), and each experiment was repeated 3 times. All data was demonstrated as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: The pNA standard curve was determined according to the Caspase3 enzyme activity assay kit (C1157, Beyotime), and after preparing the sample system according to the kit, the OD value at 405 nm was measured using an enzyme marker.

Techniques: Fluorescence, Enzyme Activity Assay, Staining, Flow Cytometry, Expressing

Journal: Heliyon

Article Title: Integrating network pharmacology and experimental verification strategies to reveal the active ingredients and molecular mechanism of Tenghuang Jiangu Capsule against osteoporosis

doi: 10.1016/j.heliyon.2023.e19812

Figure Lengend Snippet: THJGC regulates OVX-induced osteogenic differentiation and apoptosis in mice. (A) The protein expression levels of NFATc1, RUNX2, Rankl, and cleaved Caspase/Caspase3 in the bone tissues of mice from three groups (sham-operated group, n = 3; model group, n = 3; and THJGC group, n = 3) were analyzed by Western blotting, and the quantitative statistical analyses are shown in Figure (B). (C) The protein expression levels of p -Akt1/Akt1, p -ERK1/2/ERK1/2, and p -JNK1/JNK1 in the bone tissues of mice from three groups (sham-operated group, n = 3; model group, n = 3; and THJGC group, n = 3) were analyzed by Western blotting, and the quantitative statistical analyses are shown in Figure (D). All data was demonstrated as mean ± SD, *P < 0.05, **P < 0.01.

Article Snippet: The pNA standard curve was determined according to the Caspase3 enzyme activity assay kit (C1157, Beyotime), and after preparing the sample system according to the kit, the OD value at 405 nm was measured using an enzyme marker.

Techniques: Expressing, Western Blot