Journal: bioRxiv

Article Title: Fluid flow overcomes antimicrobial resistance by boosting delivery

doi: 10.1101/2024.05.08.591722

Figure Lengend Snippet: (A) H 2 O 2 is degraded by catalase into water and oxygen . (B) Gentamicin is modified by aminoglycoside-3-N-acetyltransferase that transfers the acetyl group from acetyl-CoA to the 3C amino group of gentamicin . (C) Carbenicillin is deactivated by β-lactamase that breaks the amide bond within the β-lactam ring .

Article Snippet: Carbenicillin stock solutions were prepared from carbenicillin disodium salt (Gold Biotechnology) dissolved in water.

Techniques: Modification

Journal: bioRxiv

Article Title: Fluid flow overcomes antimicrobial resistance by boosting delivery

doi: 10.1101/2024.05.08.591722

Figure Lengend Snippet: (A) Experiments measuring P. aeruginosa growth for 2 hours in M9 minimal media with 16 µM H 2 O 2 at various shear rates. (B) Experiments measuring P. aeruginosa growth for 4 hours in LB media with 100 µg/mL gentamicin at various shear rates. (C) Experiments measuring P. aeruginosa growth for 4 hours in LB media with 30 µg/mL carbenicillin at various shear rates. Higher shear rates increase effectiveness of H 2 O 2 , gentamicin, and carbenicillin against resistant P. aeruginosa strains. We interpret that growth gradients for gentamicin and carbenicillin have flatter slopes than H 2 O 2 because H 2 O 2 is smaller and has a higher diffusion coefficient. All experiments were performed in 27 cm long channels. For each biological replicate, up to 30 cells were chosen at random for quantification. Shaded regions show SD of 3 biological replicates.

Article Snippet: Carbenicillin stock solutions were prepared from carbenicillin disodium salt (Gold Biotechnology) dissolved in water.

Techniques: Shear, Diffusion-based Assay

Journal: bioRxiv

Article Title: Fluid flow overcomes antimicrobial resistance by boosting delivery

doi: 10.1101/2024.05.08.591722

Figure Lengend Snippet: P. aeruginosa cell growth over 4 hours in LB media supplemented with various concentrations of carbenicillin. Growth measurements with 800 sec -1 flow (orange) and without flow (grey) show cell growth is reduced in flow across all concentrations. For each biological replicate, 30 cells were chosen at random for quantification. Shaded regions show SD of 3 biological replicates.

Article Snippet: Carbenicillin stock solutions were prepared from carbenicillin disodium salt (Gold Biotechnology) dissolved in water.

Techniques:

Journal: bioRxiv

Article Title: Fluid flow overcomes antimicrobial resistance by boosting delivery

doi: 10.1101/2024.05.08.591722

Figure Lengend Snippet: (A) P. aeruginosa cells exposed to increasing concentrations of carbenicillin are longer. Scale bar is 5 µm. (B) Quantification of the relationship between P. aeruginosa cell length and carbenicillin concentration. For each biological replicate, 30 cells were chosen at random for quantification. Shaded regions show SD of 3 biological replicates.

Article Snippet: Carbenicillin stock solutions were prepared from carbenicillin disodium salt (Gold Biotechnology) dissolved in water.

Techniques: Concentration Assay