Journal: Veterinary Medicine and Science

Article Title: Enhanced expression of cyclooxygenase‐2 related multi‐drug resistance gene in melanoma and osteosarcoma cell lines by TSG‐6 secreted from canine adipose‐derived mesenchymal stem/stromal cells

doi: 10.1002/vms3.442

Figure Lengend Snippet: cADSCs increased COX‐2 expression in cancer cell lines. Co‐culture with cADSCs increased COX‐2 protein levels of (a) canine melanoma (LMeC) and (b) osteosarcoma (D17) cell lines. The COX‐2 protein level increased as the proportion of cADSC increased. Experiments were performed in triplicate and repeated three times with similar results. Results are shown as mean ± SD (* p < .05, ** p < .01, *** p < .001 by one‐way ANOVA analysis)

Article Snippet: Canine malignant melanoma cell line LMeC (RRID: CVCL_L361) was provided by Professor Nobuo Sasaki, University of Tokyo. (Inoue et al., ( )) Canine osteosarcoma cell line D17 [American Type Culture Collection (ATCC) CCL‐183] was obtained from the Laboratory of Veterinary Pharmacology, Seoul National University.

Techniques: Expressing, Co-Culture Assay

Journal: Veterinary Medicine and Science

Article Title: Enhanced expression of cyclooxygenase‐2 related multi‐drug resistance gene in melanoma and osteosarcoma cell lines by TSG‐6 secreted from canine adipose‐derived mesenchymal stem/stromal cells

doi: 10.1002/vms3.442

Figure Lengend Snippet: Drug resistance gene expression in cancer cell lines increased in the presence of cADSCs, and decreased with interference of COX‐2. COX‐2 protein levels in LMeC (a) and D17 (b) cancer cell lines. mRNA expression levels of COX‐2, MRP1, P‐gp in LMeC (c) and D17 (d) cancer cell lines. COX‐2 and drug resistance gene MRP1, P‐gp expression of cancer cell lines increased significantly with the presence of cADSCs . When COX‐2 was down regulated with COX‐2 targeted siRNA (siCOX‐2) in cancer cell lines, MRP1 and P‐gp expression in LMeC, D17 cells was decreased even when cultured with cADSCs. Experiments were performed in triplicate and repeated three times with similar results. Results are shown as mean ± standard deviation (* p < .05, ** p < .01, *** p < .001 by one‐way ANOVA analysis)

Article Snippet: Canine malignant melanoma cell line LMeC (RRID: CVCL_L361) was provided by Professor Nobuo Sasaki, University of Tokyo. (Inoue et al., ( )) Canine osteosarcoma cell line D17 [American Type Culture Collection (ATCC) CCL‐183] was obtained from the Laboratory of Veterinary Pharmacology, Seoul National University.

Techniques: Expressing, Cell Culture, Standard Deviation

Journal: Veterinary Medicine and Science

Article Title: Enhanced expression of cyclooxygenase‐2 related multi‐drug resistance gene in melanoma and osteosarcoma cell lines by TSG‐6 secreted from canine adipose‐derived mesenchymal stem/stromal cells

doi: 10.1002/vms3.442

Figure Lengend Snippet: TSG‐6 secreted from cADSC elevated the expression of COX‐2 in cancer cell lines. mRNA expression levels of COX‐2 in LMeC (a), and D17 (b) cell lines. Protein levels of COX‐2 in LMeC (c) and D17 (d) cell lines. COX‐2 mRNA and protein expression in cancer cell lines decreased significantly when they were co‐cultured with TSG‐6 targeted siRNA (siTSG‐6) transfected cADSCs. Experiments were performed in triplicate and repeated three times with similar results. Results are shown as mean ± SD (* p < .05, ** p < .01, *** p < .001 by one‐way ANOVA analysis)

Article Snippet: Canine malignant melanoma cell line LMeC (RRID: CVCL_L361) was provided by Professor Nobuo Sasaki, University of Tokyo. (Inoue et al., ( )) Canine osteosarcoma cell line D17 [American Type Culture Collection (ATCC) CCL‐183] was obtained from the Laboratory of Veterinary Pharmacology, Seoul National University.

Techniques: Expressing, Cell Culture, Transfection

Journal: Veterinary Medicine and Science

Article Title: Enhanced expression of cyclooxygenase‐2 related multi‐drug resistance gene in melanoma and osteosarcoma cell lines by TSG‐6 secreted from canine adipose‐derived mesenchymal stem/stromal cells

doi: 10.1002/vms3.442

Figure Lengend Snippet: TSG‐6 secreted from cADSCs affected drug resistance genes in cancer cell lines. mRNA expression levels of MRP1 and P‐gp in LMeC (a) and D17 (b) cell lines. Experiments were performed in triplicate and repeated three times with similar results. Results are shown as mean ± standard deviation (* p < .05, ** p < .01, *** p < .001 by one‐way ANOVA analysis)

Article Snippet: Canine malignant melanoma cell line LMeC (RRID: CVCL_L361) was provided by Professor Nobuo Sasaki, University of Tokyo. (Inoue et al., ( )) Canine osteosarcoma cell line D17 [American Type Culture Collection (ATCC) CCL‐183] was obtained from the Laboratory of Veterinary Pharmacology, Seoul National University.

Techniques: Expressing, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Serum Starvation-Induced Voltage-Gated Potassium Channel Kv7.5 Expression and Its Regulation by Sp1 in Canine Osteosarcoma Cells

doi: 10.3390/ijms15010977

Figure Lengend Snippet: RT-PCR analysis of the KCNQ gene family. PCR products using cDNA from the CCL-183 cell line ( A ) and dog cerebral cortex ( B ) were electrophoresed on a 2% agarose gel. M, DNA ladder.

Article Snippet: The canine osteosarcoma cell line CCL-183 was obtained from the American Type Culture Collection (Rockville, MD, USA).

Techniques: Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis

Journal: International Journal of Molecular Sciences

Article Title: Serum Starvation-Induced Voltage-Gated Potassium Channel Kv7.5 Expression and Its Regulation by Sp1 in Canine Osteosarcoma Cells

doi: 10.3390/ijms15010977

Figure Lengend Snippet: Kv7.5 knockdown by siRNA transfection induces proliferation of CCL-183 cells. The effect of transient knockdown of Kv7.5 in Kv7.5 mRNA ( A ) and protein ( B ) expression in CCL-183 cells was analyzed. The values are the mean ± SEM of four independent transfections; ( C ) An MTT cell proliferation assay was performed on the cells transfected with siRNA, and increased proliferation in Kv7.5-knockdown cells was observed compared to the cells transfected with NT siRNA. The values are the mean ± SEM of 11 independent MTT assays. * p < 0.05; ** p < 0.01.

Article Snippet: The canine osteosarcoma cell line CCL-183 was obtained from the American Type Culture Collection (Rockville, MD, USA).

Techniques: Transfection, Expressing, MTT Cell Proliferation