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canine osa cell line d17  (ATCC)


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    ATCC canine osa cell line d17
    Canine Osa Cell Line D17, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/canine osa cell line d17/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    canine osa cell line d17 - by Bioz Stars, 2024-11
    93/100 stars

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    ATCC canine osa cell line d17
    Canine Osa Cell Line D17, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC canine osa cell line d 17
    Canine Osa Cell Line D 17, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/canine osa cell line d 17/product/ATCC
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    ATCC canine d17 osa cell line
    Treatment with curcumin or FLLL32 decreased the proliferation of <t>OSA</t> cell lines . A) Canine (OSA8, OSA16, and <t>D17)</t> or human OSA cell lines (SJSA and U2OS) were treated with vehicle, curcumin, or FLLL32 for 72 hours. Proliferation was analyzed using the CyQUANT ® cell proliferation assay kit. Proliferation values are listed as a percentage of DMSO control. Experiments were performed in quadruplicate and repeated two times. Statistical analysis of cell proliferation was performed using the Student's t-test. P values of < 0.05 were considered statistically significant.* p < 0.05 B) Canine and human OSA cell lines were treated with DMSO or FLLL32 for 72 hours and analyzed with CyQUANT ® to determine proliferation as a percentage of DMSO control. IC 50 s for FLLL32 were calculated for each cell line using a log curve. Experiments were performed in quadruplicate and repeated two times.
    Canine D17 Osa Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/canine d17 osa cell line/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    canine d17 osa cell line - by Bioz Stars, 2024-11
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    Treatment with curcumin or FLLL32 decreased the proliferation of OSA cell lines . A) Canine (OSA8, OSA16, and D17) or human OSA cell lines (SJSA and U2OS) were treated with vehicle, curcumin, or FLLL32 for 72 hours. Proliferation was analyzed using the CyQUANT ® cell proliferation assay kit. Proliferation values are listed as a percentage of DMSO control. Experiments were performed in quadruplicate and repeated two times. Statistical analysis of cell proliferation was performed using the Student's t-test. P values of < 0.05 were considered statistically significant.* p < 0.05 B) Canine and human OSA cell lines were treated with DMSO or FLLL32 for 72 hours and analyzed with CyQUANT ® to determine proliferation as a percentage of DMSO control. IC 50 s for FLLL32 were calculated for each cell line using a log curve. Experiments were performed in quadruplicate and repeated two times.

    Journal: BMC Cancer

    Article Title: The novel curcumin analog FLLL32 decreases STAT3 DNA binding activity and expression, and induces apoptosis in osteosarcoma cell lines

    doi: 10.1186/1471-2407-11-112

    Figure Lengend Snippet: Treatment with curcumin or FLLL32 decreased the proliferation of OSA cell lines . A) Canine (OSA8, OSA16, and D17) or human OSA cell lines (SJSA and U2OS) were treated with vehicle, curcumin, or FLLL32 for 72 hours. Proliferation was analyzed using the CyQUANT ® cell proliferation assay kit. Proliferation values are listed as a percentage of DMSO control. Experiments were performed in quadruplicate and repeated two times. Statistical analysis of cell proliferation was performed using the Student's t-test. P values of < 0.05 were considered statistically significant.* p < 0.05 B) Canine and human OSA cell lines were treated with DMSO or FLLL32 for 72 hours and analyzed with CyQUANT ® to determine proliferation as a percentage of DMSO control. IC 50 s for FLLL32 were calculated for each cell line using a log curve. Experiments were performed in quadruplicate and repeated two times.

    Article Snippet: The canine D17 OSA cell line and human OSA cell lines U2OS and SJSA were purchased from American Type Cell Culture Collection (ATCC, Manassas, VA).

    Techniques: CyQUANT Assay, Proliferation Assay

    FLLL32 induced activation of caspase 3/7, PARP cleavage, and apoptosis of OSA cell lines . A) Canine (OSA8, OSA16, and D17) and human (SJSA and U2OS) OSA cell lines were treated with media, DMSO, curcumin, or FLLL32 for 24 hours. Apoptosis was assessed by measuring active caspase-3/7 using the SensoLyte ® Homogeneous AMC Caspase-3/7 Assay kit. Experiments were performed in triplicate and repeated two times. Statistical analysis of the caspase 3/7 activity was performed using the Student's t-test. P values of < 0.05 were considered statistically significant.* p < 0.05 B) Canine (OSA8, OSA16, and D17) or human (SJSA and U2OS) OSA cell lines were treated with DMSO, curcumin, or FLLL32 for 24 hours prior to collection. Protein lysates were generated and separated by SDS-PAGE and western blotting for PARP and β-actin was performed. Experiments were repeated two times.

    Journal: BMC Cancer

    Article Title: The novel curcumin analog FLLL32 decreases STAT3 DNA binding activity and expression, and induces apoptosis in osteosarcoma cell lines

    doi: 10.1186/1471-2407-11-112

    Figure Lengend Snippet: FLLL32 induced activation of caspase 3/7, PARP cleavage, and apoptosis of OSA cell lines . A) Canine (OSA8, OSA16, and D17) and human (SJSA and U2OS) OSA cell lines were treated with media, DMSO, curcumin, or FLLL32 for 24 hours. Apoptosis was assessed by measuring active caspase-3/7 using the SensoLyte ® Homogeneous AMC Caspase-3/7 Assay kit. Experiments were performed in triplicate and repeated two times. Statistical analysis of the caspase 3/7 activity was performed using the Student's t-test. P values of < 0.05 were considered statistically significant.* p < 0.05 B) Canine (OSA8, OSA16, and D17) or human (SJSA and U2OS) OSA cell lines were treated with DMSO, curcumin, or FLLL32 for 24 hours prior to collection. Protein lysates were generated and separated by SDS-PAGE and western blotting for PARP and β-actin was performed. Experiments were repeated two times.

    Article Snippet: The canine D17 OSA cell line and human OSA cell lines U2OS and SJSA were purchased from American Type Cell Culture Collection (ATCC, Manassas, VA).

    Techniques: Activation Assay, Activity Assay, Generated, SDS Page, Western Blot

    Downregulation of STAT3 via FLLL32 treatment decreased expression of VEGF, MMP2, and survivin . A) Canine (OSA8) or human (SJSA) OSA cell lines were treated with DMSO, 10 μM curcumin, or 10 μM FLLL32 for 12 or 24 hours. RNA was collected and RT-PCR was performed for VEGF, MMP2, and GAPDH. Experiments were repeated two times. B) Canine (OSA8, OSA16, and D17) or human (SJSA and U2OS) OSA cell lines were treated with DMSO, curcumin, or FLLL32 for 24 hours prior to collection. Protein lysates were generated and separated by SDS-PAGE and western blotting for survivin, VEGF, and β-actin was performed. Experiments were repeated two times.

    Journal: BMC Cancer

    Article Title: The novel curcumin analog FLLL32 decreases STAT3 DNA binding activity and expression, and induces apoptosis in osteosarcoma cell lines

    doi: 10.1186/1471-2407-11-112

    Figure Lengend Snippet: Downregulation of STAT3 via FLLL32 treatment decreased expression of VEGF, MMP2, and survivin . A) Canine (OSA8) or human (SJSA) OSA cell lines were treated with DMSO, 10 μM curcumin, or 10 μM FLLL32 for 12 or 24 hours. RNA was collected and RT-PCR was performed for VEGF, MMP2, and GAPDH. Experiments were repeated two times. B) Canine (OSA8, OSA16, and D17) or human (SJSA and U2OS) OSA cell lines were treated with DMSO, curcumin, or FLLL32 for 24 hours prior to collection. Protein lysates were generated and separated by SDS-PAGE and western blotting for survivin, VEGF, and β-actin was performed. Experiments were repeated two times.

    Article Snippet: The canine D17 OSA cell line and human OSA cell lines U2OS and SJSA were purchased from American Type Cell Culture Collection (ATCC, Manassas, VA).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Generated, SDS Page, Western Blot

    Treatment with FLLL32 decreased pSTAT3 and total STAT3 expression in canine and human OSA cell lines . A) Canine (OSA8, OSA16, and D17) or human (SJSA and U2OS) OSA cell lines were treated with DMSO, curcumin, or FLLL32 for 24 hours prior to collection. Protein lysates were generated and separated by SDS-PAGE and western blotting for pSTAT3 (Y705), total STAT3, and β-actin was performed. Experiments were repeated two times. B) Canine (OSA8 and 16) or human (SJSA) OSA cell lines were treated with DMSO, 10 μM curcumin, or 10 μM FLLL32 for 24 hours. RNA was collected and RT-PCR was performed for STAT3 and GAPDH. Experiments were repeated two times. C) Canine OSA cell line OSA8 was treated with DMSO, curcumin, or FLLL32 for 24 hours prior to collection. Protein lysates were generated and separated by SDS-PAGE and Western blotting for ubiquitin, STAT3, and β-actin was performed. Experiments were repeated two times. D) OSA8 was treated with DMSO or FLLL32 for 4 hours prior to collection. Protein lysates were generated and STAT3 was immunoprecipitated. Protein was separated by SDS-PAGE and western blotting for ubiquitin and STAT3 was performed. Experiments were repeated two times.

    Journal: BMC Cancer

    Article Title: The novel curcumin analog FLLL32 decreases STAT3 DNA binding activity and expression, and induces apoptosis in osteosarcoma cell lines

    doi: 10.1186/1471-2407-11-112

    Figure Lengend Snippet: Treatment with FLLL32 decreased pSTAT3 and total STAT3 expression in canine and human OSA cell lines . A) Canine (OSA8, OSA16, and D17) or human (SJSA and U2OS) OSA cell lines were treated with DMSO, curcumin, or FLLL32 for 24 hours prior to collection. Protein lysates were generated and separated by SDS-PAGE and western blotting for pSTAT3 (Y705), total STAT3, and β-actin was performed. Experiments were repeated two times. B) Canine (OSA8 and 16) or human (SJSA) OSA cell lines were treated with DMSO, 10 μM curcumin, or 10 μM FLLL32 for 24 hours. RNA was collected and RT-PCR was performed for STAT3 and GAPDH. Experiments were repeated two times. C) Canine OSA cell line OSA8 was treated with DMSO, curcumin, or FLLL32 for 24 hours prior to collection. Protein lysates were generated and separated by SDS-PAGE and Western blotting for ubiquitin, STAT3, and β-actin was performed. Experiments were repeated two times. D) OSA8 was treated with DMSO or FLLL32 for 4 hours prior to collection. Protein lysates were generated and STAT3 was immunoprecipitated. Protein was separated by SDS-PAGE and western blotting for ubiquitin and STAT3 was performed. Experiments were repeated two times.

    Article Snippet: The canine D17 OSA cell line and human OSA cell lines U2OS and SJSA were purchased from American Type Cell Culture Collection (ATCC, Manassas, VA).

    Techniques: Expressing, Generated, SDS Page, Western Blot, Reverse Transcription Polymerase Chain Reaction, Immunoprecipitation