breast cancer cell line mcf  (ATCC)

Journal: Molecular Medicine Reports

Article Title: Exploring the therapeutic potential of rabdoternin E in lung cancer treatment: Targeting the ROS/p38 MAPK/JNK signaling pathway

doi: 10.3892/mmr.2024.13330

Figure Lengend Snippet: Proliferation inhibition of rabdoternin E on A549 cells in vitro . (A) Chemical structure of rabdoternin E. (B) Time-dependent cytotoxicity of rabdoternin E on A549 cells. (C) Effect of rabdoternin E and cisplatin treatment for 24 h on A549 cell viability. (D) Cytotoxicity of rabdoternin E on human embryonic lung fibroblast MRC-5 cells. (E) Effect of rabdoternin E on the clonogenic ability of A549 cells. (F) Effect of rabdoternin E on clonogenic ability of A549 cells (x – ±s; n=6) *P<0.05, **P<0.01 vs. Con group. Con, control.

Article Snippet: The lung cancer cell line A549 was cultured in Dulbecco's Modified Eagle's Medium (Beijing Solarbio Science & Technology Co., Ltd.) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.) at 37°C in a 5% CO 2 environment.

Techniques: Inhibition, In Vitro, Control

Journal: Molecular Medicine Reports

Article Title: Exploring the therapeutic potential of rabdoternin E in lung cancer treatment: Targeting the ROS/p38 MAPK/JNK signaling pathway

doi: 10.3892/mmr.2024.13330

Figure Lengend Snippet: Rabdoternin E arrests the cell cycle in the S phase and induces apoptosis. (A) Effect of rabdoternin E on the cell cycle of A549 cells. (B) Effect of rabdoternin E on apoptosis of A549 cells. (C) With a rising concentration of rabdoternin E, the percentage of cells in S phase increased. (D) With increasing rabdoternin E concentration, the proportion of apoptotic cells gradually increased. (E) Effect of rabdoternin E on the expression of Cyclin A2, CDK2, Bcl-2 and Bax proteins in A549 cells. (F) Protein Cyclin A2 was dose-dependently downregulated (G) Protein CDK2 is dose-dependently downregulated. (H) The anti-apoptotic protein Bcl-2 was dose-dependently downregulated. (I) Pro-apoptotic protein Bax was dose-dependently upregulated. (J) Effect of co-culture using rabdoternin E and Z-VAD-FMK on cell viability. (x – ±s; n=6) *P<0.05, **P<0.01, vs. Con group; ## P<0.01, vs. apoptosis inhibitor only group. PI, propidium iodide; Con, control.

Article Snippet: The lung cancer cell line A549 was cultured in Dulbecco's Modified Eagle's Medium (Beijing Solarbio Science & Technology Co., Ltd.) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.) at 37°C in a 5% CO 2 environment.

Techniques: Concentration Assay, Expressing, Co-Culture Assay, Control

Journal: Molecular Medicine Reports

Article Title: Exploring the therapeutic potential of rabdoternin E in lung cancer treatment: Targeting the ROS/p38 MAPK/JNK signaling pathway

doi: 10.3892/mmr.2024.13330

Figure Lengend Snippet: Effect of Rab E on ferroptosis of A549 cells. (A) Effects of different concentrations (5, 10 and 15 µM) of Rab E on intracellular ROS levels in A549 cells and (B) quantitative analysis. Effect of Rab E treatment for 24 h on the expression of (C) GSH and (D) MDA as indicators associated with ferroptosis. (E) Protein expression of GPX4, FTH and SLC7A11 in A549 cells treated with Rab E. (F-H) Effect of Rab E on the protein expression of (F) GPX4, (G) FTH and (H) SLC7A11 in A549 cells. (I) Co-culture using ferroptosis-inhibiting Fer-1 and Rab E significantly reversed cell viability in A549 cells. (x – ±s; n=6) *P<0.05, **P<0.01, vs. Con group; ## P<0.01, vs. Fer-1 group with ferroptosis inhibitor only. ROS, reactive oxygen species; GSH, glutathione; MDA, malondialdehyde; GPX4, glutathione peroxidase; FTH, ferritin heavy; SLC7A11, solute carrier family 7 member 11; Rab E, rabdoternin E; Fer-1, ferrostatin-1; Con, control.

Article Snippet: The lung cancer cell line A549 was cultured in Dulbecco's Modified Eagle's Medium (Beijing Solarbio Science & Technology Co., Ltd.) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.) at 37°C in a 5% CO 2 environment.

Techniques: Expressing, Co-Culture Assay, Control

Journal: Molecular Medicine Reports

Article Title: Exploring the therapeutic potential of rabdoternin E in lung cancer treatment: Targeting the ROS/p38 MAPK/JNK signaling pathway

doi: 10.3892/mmr.2024.13330

Figure Lengend Snippet: Effect of Rab E on ROS-dependent p38 MAPK/JNK Pathway. (A) Effect of Rab E on p-p38 MAPK and JNK protein expression in A549 cells. Effect of different concentrations (5, 10 and 15 µmol/l) of Rab E on (B) p38 MAPK and (C) JNK protein expression in A549 cells. (D) Expression levels of p38 MAPK and JNK after co-cultured treatment with the ROS inhibitor NAC and different concentrations of Rab E. (E and F) Effect of co-culture of rabdoternin E and inhibitor NAC on (E) JNK and (F) p38 MAPK protein expression in A549 cells. (G) Survival of A549 cells after 24 h of co-culture of NAC and Rab E. (x – ±s; n=6) **P<0.01, vs. Con group; # P<0.05, ## P<0.01, vs. the group with only the ROS inhibitor NAC. Rab E, rabdoternin E; ROS, reactive oxygen species; NAC, N-acetylcysteine; p-, phosphorylated; Con, control.

Article Snippet: The lung cancer cell line A549 was cultured in Dulbecco's Modified Eagle's Medium (Beijing Solarbio Science & Technology Co., Ltd.) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.) at 37°C in a 5% CO 2 environment.

Techniques: Expressing, Cell Culture, Co-Culture Assay, Control

Journal: Molecular Medicine Reports

Article Title: Exploring the therapeutic potential of rabdoternin E in lung cancer treatment: Targeting the ROS/p38 MAPK/JNK signaling pathway

doi: 10.3892/mmr.2024.13330

Figure Lengend Snippet: Effect of rabdoternin E on the proliferation of subcutaneous transplanted tumors in nude mice with human lung cancer A549 cells. Plots of (A) tumor appearance, (B) tumor size and (C) body weight of mice in each group. (D) Tumor weight. (E) H&E staining results of tumor tissues in each group. Magnification, ×200 and ×400. (F) Protein bands of each experimental group. Protein expression of (G) Bcl-2, (H) Bax, (I) Ki67, (J) GPX4, (K) SLC7A11 and (L) transferrin of each experimental group. (x – ±s; n=6) *P<0.05, **P<0.01, vs. Mod group. GPX4, glutathione peroxidase; SLC7A11, solute carrier family 7 member 11; Mod, model group.

Article Snippet: The lung cancer cell line A549 was cultured in Dulbecco's Modified Eagle's Medium (Beijing Solarbio Science & Technology Co., Ltd.) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.) at 37°C in a 5% CO 2 environment.

Techniques: Staining, Expressing

Journal: Molecular Medicine Reports

Article Title: Exploring the therapeutic potential of rabdoternin E in lung cancer treatment: Targeting the ROS/p38 MAPK/JNK signaling pathway

doi: 10.3892/mmr.2024.13330

Figure Lengend Snippet: Mechanism of rabdoternin E inhibiting proliferation of A549 cells. p-, phosphorylated; GPX4, glutathione peroxidase; SLC7A11, solute carrier family 7 member 11.

Article Snippet: The lung cancer cell line A549 was cultured in Dulbecco's Modified Eagle's Medium (Beijing Solarbio Science & Technology Co., Ltd.) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.) at 37°C in a 5% CO 2 environment.

Techniques: