cancer stem cell medium  (PromoCell)

Journal: Molecular Cancer Therapeutics

Article Title: 18 F-FLT PET, a Noninvasive Pharmacodynamic Biomarker of Tumor Cell Proliferation, Detected Differential Response to Various Cyclin-Dependent Kinase (CDK) Inhibitors

doi: 10.1158/1535-7163.MCT-24-0856

Figure Lengend Snippet: Tumor growth response of MCF7 and OVCAR-3 toward palbociclib and the CDK2-specific inhibitor PF-07104091 (compound 1). MCF7 and OVCAR-3 tumors were established on the subcutaneous flank of NOD/SCID gamma mice. Drug treatment was initiated when average tumor volume reached approximately 200 mm 3 . Both palbociclib and compound 1 were administered orally at the dose levels, frequency, and duration indicated in the graphs. Data shown are mean tumor volume ± SEM. TGI was determined on the last day of treatment. A, MCF7 tumors reached 74% TGI upon palbociclib treatment ( P < 0.05). B , OVCAR-3 tumors showed a mere 22% TGI upon palbociclib treatment (not statistically significant). C , MCF7 tumors showed 52%, 64%, and 82% TGI in response to compound 1 dose escalation ( P < 0.05 for all groups; all comparisons made against vehicle control). D , OVCAR-3 tumors reached 54%, 61%, and 85% TGI in response to compound 1 dose escalation ( P < 0.05 for all groups; all comparisons made against vehicle control). For A–D , N = 10/group. Statistical analysis was done by analysis of covariance.

Article Snippet: Exogenous estrogen supplementation is required to facilitate MCF7 tumor growth, which was provided by either E2 pellet (0.36 mg, 90-day release, Innovative Research of America) implantation 2 days before tumor inoculation or E2 water (8.5 μg/mL) provided 1 week before tumor implantation.

Techniques: Control

Journal: Molecular Cancer Therapeutics

Article Title: 18 F-FLT PET, a Noninvasive Pharmacodynamic Biomarker of Tumor Cell Proliferation, Detected Differential Response to Various Cyclin-Dependent Kinase (CDK) Inhibitors

doi: 10.1158/1535-7163.MCT-24-0856

Figure Lengend Snippet: 18 F-FLT PET imaging detected CDK and cell-cycle inhibition in the MCF7 xenograft model. A, Representative transverse multiplanar reformation (top) and coronal, whole-body maximal intensity projection images (bottom) of MCF7 tumor–bearing mice treated with the indicated CDK inhibitors. Red arrows: tumor; yellow arrows: gastrointestinal tract; and white arrows: gall bladder. All images are scaled equally. B, Region of interest (ROI) analysis quantitation (SUVmax) of tumor uptake of 18 F-FLT. C, Representative images of IHC staining against Ki67 in MCF7 tumors treated with palbociclib. D, Quantitation (H-score) of Ki67 staining. E, Representative images of IHC staining against pRb in the same experiment. F, Quantitation (H-score) of pRb IHC staining. Data are shown in mean ± SEM. N = 6 to 12/group. Statistical analysis was done by an unpaired t test. Scale bar, 25 µm.

Article Snippet: Exogenous estrogen supplementation is required to facilitate MCF7 tumor growth, which was provided by either E2 pellet (0.36 mg, 90-day release, Innovative Research of America) implantation 2 days before tumor inoculation or E2 water (8.5 μg/mL) provided 1 week before tumor implantation.

Techniques: Imaging, Inhibition, Quantitation Assay, Immunohistochemistry, Staining

Journal: International Journal of Nanomedicine

Article Title: Multifunctional Poly(Acrylic Acid)-Coated EuBiGd 2 O 3 Nanocomposite as an Effective Contrast Agent in Spectral Photon Counting CT, MRI, and Fluorescence Imaging

doi: 10.2147/IJN.S506187

Figure Lengend Snippet: ( a ) A micrographs of A549 cells without NPs treatment and after treatment with PAA@EuBiGd 2 O 3 -NPs with concentrations of ( b ) 32.5 µg/mL, ( c ) 250 µg/mL, and ( d ) 1000 µg/mL, for 24 hrs. ( e ) CellTiter-glo based in vitro A549 cells viability studies in the presence of PAA@EuBiGd 2 O 3 -NPs for 24 hrs and 48 hrs. ( f ) Evaluation of IC50 after 24 hrs (365.8 µg/mL) and 48 hrs (337.8 µg/mL) treatment. The fitting correlation, R 2 , values were 0.9735 and 0.9588 for 24 and 48 hrs incubation.

Article Snippet: Human lung cancer cell line A549 was purchased from American Type Culture Collection (ATCC) and cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Sigma) supplemented with 10% (v/v) of heat-inactivated fetal bovine serum (Sigma) and maintained at 37 °C in a fully humidified atmosphere containing 5 % CO 2 .

Techniques: In Vitro, Incubation

Journal: International Journal of Nanomedicine

Article Title: Multifunctional Poly(Acrylic Acid)-Coated EuBiGd 2 O 3 Nanocomposite as an Effective Contrast Agent in Spectral Photon Counting CT, MRI, and Fluorescence Imaging

doi: 10.2147/IJN.S506187

Figure Lengend Snippet: Confocal laser scanning microscopy images of ( a – c ) Luminescence of nanoparticles, DAPI-stained nucleus, and the merged image of untreated A549 cells. ( d – f ) Luminescence of nanoparticles, DAPI-stained nucleus, and the merged image of A549 cells treated with PAA@EuBiGd 2 O 3 NPs. ( g ) Magnified view of ( f ), highlighting nanoparticle localization.

Article Snippet: Human lung cancer cell line A549 was purchased from American Type Culture Collection (ATCC) and cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Sigma) supplemented with 10% (v/v) of heat-inactivated fetal bovine serum (Sigma) and maintained at 37 °C in a fully humidified atmosphere containing 5 % CO 2 .

Techniques: Confocal Laser Scanning Microscopy, Staining